Partial Characterization of Hydrolytic Enzymes Produced by Bacillus Strains Isolated from Balıklıgöl, Turkey

Ebru Uyar; Cengiz Çorbacı

doi:10.18016/ksutarimdoga.vi.809131

TR EN

Balıklıgöl, Türkiye’den İzole Edilen Bacillus Suşları Tarafından Üretilen Hidrolitik Enzimlerin Kısmi Karakterizasyonu

Öz

Bu çalışmada, Balıklıgöl, Şanlıurfa’dan elde edilmiş ve daha önceden çalışılmamış toprak örneklerinden kırk beş bakteriyel izolat elde edilmiştir. Enzim üretim kapasitelerine göre bu izolatlar arasından BGL-22, BGL-26, BGL-27, BGL-37, BGL-38 ve BGL-39 olarak isimlendirilen altı bakteriyal izolat ileriki denemeler için seçilmiştir. Konvansiyonel ve moleküler tanılama sonuçları, bu bakterilerin Bacillus cinsine dahil olduğunu göstermiştir. Bu suşlar arasından Bacillus sp. BGL-37, amilaz (11.44 U mL-1), lipaz (1.12 U mL-1) ve proteaz (2.61 U mL-1) açısından en yüksek aktiviteyi göstermiştir. Enzimatik karakterizasyon çalışmaları, alkali proteazın 40ºC’ye kadar yaklaşık %90 aktivitesini koruduğunu, asit-kararlı amilaz ve alkali-kararlı lipazın ise 50ºC’ye kadar aktivitelerinde herhangi bir düşüş yaşanmadan aktivite gösterdiklerini ortaya koymuştur. Elde edilen bulgular, nispeten ılımlı koşullar altında çevresel olarak uyumlu süreçlerin gerçekleşmesini sağlayan bu enzimlerin, gıdaların işlenmesi ve deterjan endüstrisi gibi çeşitli alanlarda kullanılma potansiyeline sahip olduğunu göstermiştir.

Anahtar Kelimeler

Partial Characterization of Hydrolytic Enzymes Produced by Bacillus Strains Isolated from Balıklıgöl, Turkey

Abstract

In the present study, forty-five bacterial isolates were obtained from previously unstudied soil samples in Balıklıgöl, Şanlıurfa. Based on their enzim production capacities, six bacterial isolates designated as BGL-22, BGL-26, BGL-27, BGL-37, BGL-38 and BGL-39 were selected for further studies. Conventional and molecular identification results showed that the bacteria belonged to Bacillus genus. Among these strains, the highest activities for amylase (11.44 U mL-1), lipase (1.12 U mL-1) and protease (2.61 U mL-1) were determined for Bacillus sp. BGL-37. Enzymatic characterization studies demonstrated that the activities of acid-stable amylase and alkaline-stable lipase remained unchanged up to 50℃, while alkaline-protease was retained about 90% of its activity up to 40℃. The findings suggested that these enzymes providing environmentally compatible processes under relatively mild conditions have potential to be used in several fields such as food processing and detergent industry.

Keywords

Supporting Institution

the Scientific Research Unit of Harran University

Project Number

Project No: HUBAK-12007

Thanks

This study was supported by the Scientific Research Unit of Harran University (Project No: HUBAK-12007). We also thank MedSanTek for performing sequence analysis of PCR products.

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Ebru Uyar ^*
0000-0002-4022-3845
Türkiye

Cengiz Çorbacı
0000-0001-8697-0945
Türkiye

Publication Date

August 31, 2021

Submission Date

October 12, 2020

Acceptance Date

December 3, 2020

Published in Issue

Year 2021 Volume: 24 Number: 4

DOI

https://doi.org/10.18016/ksutarimdoga.vi.809131

IZ

https://izlik.org/JA78WX73GB

Cite

RIS / Bibtex

APA

Uyar, E., & Çorbacı, C. (2021). Partial Characterization of Hydrolytic Enzymes Produced by Bacillus Strains Isolated from Balıklıgöl, Turkey. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 24(4), 707-714. https://doi.org/10.18016/ksutarimdoga.vi.809131