The Isolation and Characterization of Phosphate Solubilizing Bacteria from the Onion Rhizosphere and Their Effect on Onion Growth

İdris Bektaş; Mustafa Küsek

doi:10.18016/ksutarimdoga.vi.811324

TR EN

Soğan (Allium cepa L.) Rizosferindeki Fosfat Çözen Bakterilerin İzolasyonu, Karakterisasyonu ve Soğan Gelişimi Üzerine Etkisi

Abstract

Bu çalışmada 269 fosfat çözen bakteri(PSB) izolatı soğan rizosfer bölgesinden izole edilmiştir. İzolatlar arasından en fazla fosfat çözen 15 bakteri seçilmiştir. BIOLOG test profiline ve LOPAT test sistemine göre seçilen izolatlar Enterobacter cloacae(7), Yersinia intermedia, Pantoea agglomerans, Bacillus subtilis(2), Bacillus pumilius, Lysinibacillus sphaericus ve Bacillus atrophaeus(2) olarak tanılanmıştır. Bu izolatların soğan gelişi üzerine etkisi sera koşullarında incelenmiştir. Seçilen bu izolatlar kontrole göre, bitki boyunu %11.7-38.8, yaprak sayısını %3-70, yumru çapını %6.4-64.5, ortalama kök uzunluğunu %2.6-44.7, soğan yumru yaş ağırlığını %2.9-34.1 ve soğan yumru kuru ağırlığını %1.5-40.6 arasında artırdıkları belirlenmiştir. P çözen izolatlar arasındaki E. cloacae EB-14 ve E. cloacae OB-169 tüm paramertreleri ticari bir bitki aktivatörü ürün olan ISR 2000 den istatistiki olarak daha fazla artırarak en etkili izolatlar olmuşlardır. Bu sonuçlar göstermiştir ki PSB’ler soğan gelişimi için potansiyel bir inokulant olarak kullanılabilir.

Keywords

The Isolation and Characterization of Phosphate Solubilizing Bacteria from the Onion Rhizosphere and Their Effect on Onion Growth

Abstract

In this study 269 phosphate solubilizing bacteria (PSB) strains were isolated from onions rhizosphere. The 15 most efficient PSB were selected. Based on BIOLOG profiling and the LOPAT test system, the strains were identiﬁed as Enterobacter cloacae (7), Yersinia intermedia, Pantoea agglomerans, Bacillus subtilis(2), Bacillus pumilius, Lysinibacillus sphaericus and Bacillus atrophaeus(2). The PSB effect on onion growth was investigated under greenhouse conditions. These PSB increased plant height (11.7 to 38.8%), the number of leaves (3 to 70%), the bulb diameter (6.4 to 64.5%), the average root length (2.6 to 44.7%), the fresh weight of the bulb (2.9 to 34.1%) and the dry weight of the bulb (1.5 to 40.6%) when compared to the negative control. Among these strains the most effective inoculants werethe highest P solubizing E.cloacae EB-14 and E. cloacae OB-169 strains, which significantly increased all parameters compared to the commercial plant activator ISR 2000. These results demonstrated the potential use of these PSB as inoculants for onion growth.

Keywords

Supporting Institution

Kahramanmaras Sutcu Imam University

Project Number

2014/3-28 D

Thanks

This research was supported by the project's financial support of Kahramanmaras Sutcu Imam University Scientific Research Projects (grant no. 2014/3-28 D) is gratefully acknowledged. Ph.D. thesis of İdris BEKTAŞ is partially used to produce this manuscript.

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Details

Primary Language

English

Subjects

Agricultural, Veterinary and Food Sciences

Journal Section

Research Article

Authors

İdris Bektaş ^*
0000-0001-7409-4837
Türkiye

Mustafa Küsek
0000-0002-6320-5869
Türkiye

Publication Date

October 31, 2021

Submission Date

October 16, 2020

Acceptance Date

January 7, 2021

Published in Issue

Year 2021 Volume: 24 Number: 5

DOI

https://doi.org/10.18016/ksutarimdoga.vi.811324

IZ

https://izlik.org/JA67XR73SA

Cite

RIS / Bibtex

APA

Bektaş, İ., & Küsek, M. (2021). The Isolation and Characterization of Phosphate Solubilizing Bacteria from the Onion Rhizosphere and Their Effect on Onion Growth. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 24(5), 1084-1092. https://doi.org/10.18016/ksutarimdoga.vi.811324

AMA

1.Bektaş İ, Küsek M. The Isolation and Characterization of Phosphate Solubilizing Bacteria from the Onion Rhizosphere and Their Effect on Onion Growth. KSU J. Agric Nat. 2021;24(5):1084-1092. doi:10.18016/ksutarimdoga.vi.811324

Chicago

Bektaş, İdris, and Mustafa Küsek. 2021. “The Isolation and Characterization of Phosphate Solubilizing Bacteria from the Onion Rhizosphere and Their Effect on Onion Growth”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi 24 (5): 1084-92. https://doi.org/10.18016/ksutarimdoga.vi.811324.

EndNote

Bektaş İ, Küsek M (October 1, 2021) The Isolation and Characterization of Phosphate Solubilizing Bacteria from the Onion Rhizosphere and Their Effect on Onion Growth. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 24 5 1084–1092.

IEEE

[1]İ. Bektaş and M. Küsek, “The Isolation and Characterization of Phosphate Solubilizing Bacteria from the Onion Rhizosphere and Their Effect on Onion Growth”, KSU J. Agric Nat., vol. 24, no. 5, pp. 1084–1092, Oct. 2021, doi: 10.18016/ksutarimdoga.vi.811324.

ISNAD

Bektaş, İdris - Küsek, Mustafa. “The Isolation and Characterization of Phosphate Solubilizing Bacteria from the Onion Rhizosphere and Their Effect on Onion Growth”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 24/5 (October 1, 2021): 1084-1092. https://doi.org/10.18016/ksutarimdoga.vi.811324.

JAMA

1.Bektaş İ, Küsek M. The Isolation and Characterization of Phosphate Solubilizing Bacteria from the Onion Rhizosphere and Their Effect on Onion Growth. KSU J. Agric Nat. 2021;24:1084–1092.

MLA

Bektaş, İdris, and Mustafa Küsek. “The Isolation and Characterization of Phosphate Solubilizing Bacteria from the Onion Rhizosphere and Their Effect on Onion Growth”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, vol. 24, no. 5, Oct. 2021, pp. 1084-92, doi:10.18016/ksutarimdoga.vi.811324.

Vancouver

1.İdris Bektaş, Mustafa Küsek. The Isolation and Characterization of Phosphate Solubilizing Bacteria from the Onion Rhizosphere and Their Effect on Onion Growth. KSU J. Agric Nat. 2021 Oct. 1;24(5):1084-92. doi:10.18016/ksutarimdoga.vi.811324

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