The Isolation and Characterization of Phosphate Solubilizing Bacteria from the Onion Rhizosphere and Their Effect on Onion Growth

İdris Bektaş; Mustafa Küsek

doi:10.18016/ksutarimdoga.vi.811324

TR EN

Soğan (Allium cepa L.) Rizosferindeki Fosfat Çözen Bakterilerin İzolasyonu, Karakterisasyonu ve Soğan Gelişimi Üzerine Etkisi

Öz

Bu çalışmada 269 fosfat çözen bakteri(PSB) izolatı soğan rizosfer bölgesinden izole edilmiştir. İzolatlar arasından en fazla fosfat çözen 15 bakteri seçilmiştir. BIOLOG test profiline ve LOPAT test sistemine göre seçilen izolatlar Enterobacter cloacae(7), Yersinia intermedia, Pantoea agglomerans, Bacillus subtilis(2), Bacillus pumilius, Lysinibacillus sphaericus ve Bacillus atrophaeus(2) olarak tanılanmıştır. Bu izolatların soğan gelişi üzerine etkisi sera koşullarında incelenmiştir. Seçilen bu izolatlar kontrole göre, bitki boyunu %11.7-38.8, yaprak sayısını %3-70, yumru çapını %6.4-64.5, ortalama kök uzunluğunu %2.6-44.7, soğan yumru yaş ağırlığını %2.9-34.1 ve soğan yumru kuru ağırlığını %1.5-40.6 arasında artırdıkları belirlenmiştir. P çözen izolatlar arasındaki E. cloacae EB-14 ve E. cloacae OB-169 tüm paramertreleri ticari bir bitki aktivatörü ürün olan ISR 2000 den istatistiki olarak daha fazla artırarak en etkili izolatlar olmuşlardır. Bu sonuçlar göstermiştir ki PSB’ler soğan gelişimi için potansiyel bir inokulant olarak kullanılabilir.

Anahtar Kelimeler

The Isolation and Characterization of Phosphate Solubilizing Bacteria from the Onion Rhizosphere and Their Effect on Onion Growth

Abstract

In this study 269 phosphate solubilizing bacteria (PSB) strains were isolated from onions rhizosphere. The 15 most efficient PSB were selected. Based on BIOLOG profiling and the LOPAT test system, the strains were identiﬁed as Enterobacter cloacae (7), Yersinia intermedia, Pantoea agglomerans, Bacillus subtilis(2), Bacillus pumilius, Lysinibacillus sphaericus and Bacillus atrophaeus(2). The PSB effect on onion growth was investigated under greenhouse conditions. These PSB increased plant height (11.7 to 38.8%), the number of leaves (3 to 70%), the bulb diameter (6.4 to 64.5%), the average root length (2.6 to 44.7%), the fresh weight of the bulb (2.9 to 34.1%) and the dry weight of the bulb (1.5 to 40.6%) when compared to the negative control. Among these strains the most effective inoculants werethe highest P solubizing E.cloacae EB-14 and E. cloacae OB-169 strains, which significantly increased all parameters compared to the commercial plant activator ISR 2000. These results demonstrated the potential use of these PSB as inoculants for onion growth.

Keywords

Supporting Institution

Kahramanmaras Sutcu Imam University

Project Number

2014/3-28 D

Thanks

This research was supported by the project's financial support of Kahramanmaras Sutcu Imam University Scientific Research Projects (grant no. 2014/3-28 D) is gratefully acknowledged. Ph.D. thesis of İdris BEKTAŞ is partially used to produce this manuscript.

References

Aktan ZC, Soylu S 2020. Diyarbakir İlinde Yetişen Badem Ağaçlarindan Endofit ve Epifit Bakteri Türlerinin Izolasyonu ve Bitki Gelişimini Teşvik Eden Mekanizmalarinin Karakterizasyonu. KSÜ Tarım ve Doğa Dergisi 23(3): 641-654.
Alia AA, Shahida N, Bushra J, Saeed A 2013. Phosphate Solubilizing Bacteria Associated with Vegetables Roots in Different Ecologies. Pak J Bot 45: 535-544.
Aktan ZC, Soylu S 2020. Diyarbakır İlinde Yetişen Badem Ağaçlarından Endofit ve Epifit Bakteri Türlerinin İzolasyonu ve Bitki Gelişimini Teşvik Eden Mekanizmalarının Karakterizasyonu. KSÜ Tarım ve Doğa Derg 23(3): 641-654.
Audipudi AV, Kumar NP, Sudhir A 2012. Phosphate Solubilizing Microorganisms Associated with Chollangi Mangrove Soil in East Coast of India. International Journal of Scientific & Engineering Research 3(11): 2229-5518.
Barton CJ 1948. Photometric Analysis of Phosphate Rock. Analytical Chemistry 20(11): 1068-1073.
Bhat SA, Singh J, Vig AP 2017. Earthworms as Organic Waste Managers and Biofertilizer Producers. Waste and Biomass Valorization 9(7): 1073-1086.
Biswas JK, Banerjee, A, Rai M, Naidu R, Biswas B, Vithanage M, Dash MC, Sarkar SK, Meers E 2018. Potential Application of Selected Metal Resistant Phosphate Solubilizing Bacteria Isolated from the Gut of Earthworm (Metaphire Posthuma) in Plant Growth Promotion. Geoderma 330: 117-124.
Cruz-Paredes C, López-García Á, Rubæk GH, Hovmand MF, Sørensen P, Kjøller R 2017. Risk Assessment of Replacing Conventional P Fertilizers with Biomass Ash: Residual Effects on Plant Yield, Nutrition, Cadmium Accumulation and Mycorrhizal Status. Science of the Total Environment 575: 1168-1176.

Duman K, Soylu S 2019. Characterization of Plant Growth-Promoting Traits and Antagonistic Potentials of Endophytic Bacteria from Bean Plants Against Pseudomonas syringae pv. phaseolicola. Bitki Koruma Bülteni 59(3): 59-69.
Gupta M, Kiran S, Gulati A, Singh B, Tewari R 2012. Isolation and Identification of Phosphate Solubilizing Bacteria able to Enhance the Growth and Aloin-A Biosynthesis of Aloe Barbadensis Miller. Microbiological Research 167(6): 358-363.
Dadaşoğlu F, Kotan R 2017. Bazı Sebze ve Meyvelerde Yumuşak Çürüklük Oluşturan Pektolitik Bakterilerin Tanı ve Karakterizasyonu. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 7(1): 155-161.
Hameeda B, Harini G, Rupela O, Wani S, Reddy G 2008. Growth Promotion of Maize by Phosphate-Solubilizing Bacteria Isolated from Composts and Macrofauna. Microbiological Research 163(2): 234-242.
Khan MS, Zaidi A, Ahemad M, Oves M, Wani PA 2010. Plant Growth Promotion by Phosphate Solubilizing Fungi–Current Perspective. Archives of Agronomy and Soil Science 56(1): 73-98.
Kumar A, Maurya B, Raghuwanshi R 2014. Isolation and Characterization of PGPR and their Effect on Growth, Yield and Nutrient Content In Wheat (Triticum aestivum L.).Biocatalysis and Agricultural Biotechnology 3(4): 121-128.
Kumar A, Singh R, Chhillar R 2001. Influence of Nitrogen and Potassium Application on Growth, Yield and Nutrient Uptake by Onion (Allium cepa). Indian Journal of Agronomy 46(4): 742-746.
Lompo F, Bationo A, Sedogo MP, Bado VB, Hien V, Ouattara B 2018. Role of Local Agro-minerals in Mineral Fertilizer Recommandations for Crops: Examples of Some West Africa Phosphate Rocks, Improving the Profitability, Sustainability and Efficiency of Nutrients Through Site Specific Fertilizer Recommendations in West Africa Agro-Ecosystems. Springer, pp. 157-180.
Ludueña LM, Anzuay MS, Angelini JG, McIntosh M, Becker A, Rupp O, Goesmann A, Blom J, Fabra A, Taurian T 2018. Strain Serratia sp. S119: A Potential Biofertilizer for Peanut and Maize and A Model Bacterium to Study Phosphate Solubilization Mechanisms. Applied Soil Ecology 126: 107-112.
Majeed A, Abbasi MK, Hameed S, Imran A, Rahim N 2015. Isolation and Characterization of Plant Growth-Promoting Rhizobacteria from Wheat Rhizosphere and their Effect on Plant Growth Promotion. Frontiers in Microbiology 6: 198.
Moragrega C, Llorente I, Manceau C, Montesinos E 2003. Susceptibility of European Pear Cultivars to Pseudomonas syringae pv. syringae using Immature Fruit and Detached Leaf Assays. European Journal of Plant Pathology 109(4): 319-326.
Nasri S, Anoush M, Khatami N 2012. Evaluation of Analgesic and Anti-Inflammatory Effects of Fresh Onion Juice in Experimental Animals. African Journal of Pharmacy and Pharmacology 6(23): 1679-1684.
Nautiyal CS 1999. An Efficient Microbiological Growth Medium for Screening Phosphate Solubilizing Microorganisms. FEMS Microbiology Letters 170(1): 265 270.
Premono ME, Moawad A, Vlek P 1996. Effect of Phosphate-Solubilizing Pseudomonas Putida on the Growth of Maize and its Survival in the Rhizosphere.( No. REP-12113. CIMMYT.)
Ramamoorthy V, Raguchander T, Samiyappan R 2002. Enhancing Resistance of Tomato and Hot Pepper to Pythium Diseases by Seed Treatment with Fluorescent Pseudomonads. European Journal of Plant Pathology 108(5): 429-441.
Richardson AE 2001. Prospects for using Soil Microorganisms to Improve the Acquisition of Phosphorus by Plants. Functional Plant Biology 28(9): 897-906.
Rodrı́guez H, Fraga R 1999. Phosphate Solubilizing Bacteria and their Role in Plant Growth Promotion. Biotechnology Advances 17(4-5): 319-339.
Schaad NW, Jones JB, Chun W 2001. Laboratory Guide for the Identification of Plant Pathogenic Bacteria. American Phytopathological Society (APS Press) 398 p.
Sharma SB, Sayyed RZ, Trivedi MH, Gobi TA 2013. Phosphate Solubilizing Microbes: Sustainable Approach for Managing Phosphorus Deficiency in Agricultural Soils. Springer Plus 2(1): 587.
Slimestad R, Fossen T, Vågen IM, 2007. Onions: a Source of Unique Dietary Flavonoids. Journal of Agricultural and Food Chemistry 55(25): 10067-10080.
Soylu EM, Soylu S, Kara M, Kurt Ş 2020. Sebzelerde Sorun Olan Önemli Bitki Fungal Hastalık Etmenlerine Karşı Vermikomposttan İzole Edilen Mikrobiyomların in vitro Antagonistik Etkilerinin Belirlenmesi. KSÜ Tarım ve Doğa Dergisi 23(1): 7-18.
Suri V, Choudhary AK 2013.Glycine–Glomus–Phosphate Solubilizing Bacteria Interactions Lead to Fertilizer Phosphorus Economy in Soybean in a Himalayan acid Alfisol.Communications in Soil Science and Plant Analysis 44(20): 3020-3029.
Telek Ü, Akıncı İE, Küsek M 2019. Rhizobakteri İzolatlarının Kırmızı Biberin (Capsicum annuum L.) Verim ve Bitkisel Özellikleri Üzerine Etkileri. KSÜ Tar Doğa Derg 22(1): 62-70.
Verma P, Yadav AN, Khannam KS, Panjiar N, Kumar S, Saxena AK, Suman A 2015. Assessment of Genetic Diversity and Plant Growth Promoting Attributes of Psychrotolerant Bacteria Allied with Wheat (Triticum Aestivum) from the Northern Hills Zone of India. Annals of Microbiology 65(4): 1885-1899.
Zhang J, Liu J, Meng L, Ma Z, Tang X, Cao Y, Sun L 2012. Isolation and Characterization of Plant Growth-Promoting Rhizobacteria from Wheat Roots by Wheat Germ Agglutinin Labeled with Fluorescein Isothiocyanate. The Journal of Microbiology 50(2): 191-198.
Zhang L, Feng G, Declerck S 2018. Signal Beyond Nutrient, Fructose, Exuded by an Arbuscular Mycorrhizal Fungus Triggers Phytate Mineralization by a Phosphate Solubilizing Bacterium. The ISME Journal12(10): 2339-2351.

Details

Primary Language

English

Subjects

Agricultural, Veterinary and Food Sciences

Journal Section

Research Article

Authors

İdris Bektaş ^*
0000-0001-7409-4837
Türkiye

Mustafa Küsek
0000-0002-6320-5869
Türkiye

Publication Date

October 31, 2021

Submission Date

October 16, 2020

Acceptance Date

January 7, 2021

Published in Issue

Year 2021 Volume: 24 Number: 5

DOI

https://doi.org/10.18016/ksutarimdoga.vi.811324

IZ

https://izlik.org/JA67XR73SA

Cite

RIS / Bibtex

APA

Bektaş, İ., & Küsek, M. (2021). The Isolation and Characterization of Phosphate Solubilizing Bacteria from the Onion Rhizosphere and Their Effect on Onion Growth. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 24(5), 1084-1092. https://doi.org/10.18016/ksutarimdoga.vi.811324

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