The Role of Nitrosative and Oxidative Stress in Rainbow Trout (Oncorhynchus Mykiss) Gill Tissue Applying Mercury Chloride

Mehmet Reşit Taysı; Muammer Kırıcı; Mahinur Kırıcı; Bünyamin Sögüt; Mehmet Akif Bozdayı; Mehmet Tarakçıoğlu; Seyithan Taysı

doi:10.18016/ksutarimdoga.vi.821176

TR EN

Civa Klorür Uygulanan Gökkuşağı Alabalıkları (Oncorhynchus Mykiss)’ nın Solungaç Dokusunda Nitrozatif ve Oksidatif Stresin Rolü

Öz

Çalışmanın amacı, civa klorürün (HgCl2) 59,43 ± 7,21 g ağırlığındaki gökkuşağı alabalıklarında (Oncorhynchus mykiss) toksik etkisinin ve neden olduğu oksidatif stresin dinamiklerini belirlemektir. Bu amaçla, her grupta 10 balık (n = 10) olacak şekilde 4 grupta toplam 40 balık, LD50'nin %25 ve %50'sine (137,75 μg/L ve 275 μg/L) 2 ve 7 gün süreyle maruz bırakıldı. Oksidatif / nitrozatif stresi belirlemek için, Peroksinitrit (ONOOˉ mmol / L), Toplam Oksidan Seviyesi (TOS mmol H2O2 Eq / L), Toplam Antioksidan Seviyesi (TAS mmol Trolox Eq / L), OSI (TOS / TASx10) ve Malondialdehit (MDA) seviyesi belirlendi. Solungaç dokularında TAS, OSI ve MDA düzeyleri açısından gruplar arasındaki fark istatistiksel olarak anlamlı iken (P <0.05), bu fark ONOOˉ ve TOS değerleri açısından istatistiksel olarak önemsiz çıktı (P> 0.05). Sonuç olarak, HgCl2 solungaç dokusunda stres göstergeleri olan ONOOˉ, OSI ve MDA seviyelerini arttırdığı için strese neden olduğu ve balıklar için toksik olduğu görüldü.

Anahtar Kelimeler

The Role of Nitrosative and Oxidative Stress in Rainbow Trout (Oncorhynchus Mykiss) Gill Tissue Applying Mercury Chloride

Abstract

The aim of the study was to determine the toxic effect and the dynamics of oxidative stress caused by mercury chloride (HgCl2) in rainbow trout (Oncorhynchus mykiss) weighing 59.43 ± 7.21 g.For this purpose, a total of 40 fish in 4 groups, 10 fish in each group (n = 10), were exposed to 25% and 50% (137.75 μg/L and 275 μg/L) of the LD50 for 2 and 7 days. To determine the oxidative/nitrosative stress, Peroxynitrite (ONOOˉ mmol/L), Total Oxidant Level (TOS mmol H2O2 Eq/L), Total Antioxidant Level (TAS mmol Trolox Eq/L), OSI (TOS/TASx10) and Malondialdehyde (MDA) level were determined. While the difference between the groups in terms of TAS, OSI and MDA levels in gill tissues was statistically significant (P<0.05), this difference was statistically insignificant (P>0.05) in terms of ONOOˉ and TOS values. As a result, HgCl2 was found to cause stress and toxic to fish as it increases the levels of ONOOˉ, OSI and MDA, which are indicators of stress in gill tissue.

Keywords

References

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Details

Primary Language

English

Subjects

Agricultural, Veterinary and Food Sciences

Journal Section

Research Article

Authors

Mehmet Reşit Taysı ^*
0000-0002-1072-4059
Türkiye

Muammer Kırıcı
0000-0003-1888-4388
Türkiye

Mahinur Kırıcı
0000-0003-4642-7387
Türkiye

Bünyamin Sögüt
0000-0002-7644-7226
Türkiye

Mehmet Akif Bozdayı
0000-0003-1152-0063
Türkiye

Mehmet Tarakçıoğlu
0000-0002-2398-8264
Türkiye

Seyithan Taysı
0000-0003-1251-3148
Türkiye

Publication Date

October 31, 2021

Submission Date

November 4, 2020

Acceptance Date

March 1, 2021

Published in Issue

Year 2021 Volume: 24 Number: 5

DOI

https://doi.org/10.18016/ksutarimdoga.vi.821176

IZ

https://izlik.org/JA52ZH45FP

Cite

RIS / Bibtex

APA

Taysı, M. R., Kırıcı, M., Kırıcı, M., Sögüt, B., Bozdayı, M. A., Tarakçıoğlu, M., & Taysı, S. (2021). The Role of Nitrosative and Oxidative Stress in Rainbow Trout (Oncorhynchus Mykiss) Gill Tissue Applying Mercury Chloride. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 24(5), 957-962. https://doi.org/10.18016/ksutarimdoga.vi.821176

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