Batı Karadeniz Bölgesi Kivi Bahçelerinde Kök-Ur Nematodu (Meloidogyne spp.) Yaygınlığı ve Yoğunluğu

Emre Evlice; Esengül Özdemir

doi:10.18016/ksutarimdoga.vi.846406

EN TR

Distribution and Occurrence of Root-Knot Nematodes (Meloidogyne spp.) in Kiwi Orchards in Western Black Sea Region

Abstract

Objective of this study was to determine the prevalence and density of root-knot nematodes in kiwi orchards in the Western Black Sea Region. For this purpose, root and soil samples were taken from eight kiwi orchards in Düzce (2), Zonguldak (3), and Bartın (3) provinces in 2015 and 2016. Genomic DNA of the nematodes was extracted from second stage juviniles (J2), and ClearDetections’s real-time primers were used for identification of M. tropical spp., M. hapla and M. enterelobii and specific universal MF / MR primers were used for molecular identifications of Meloidogyne spp. As a result of the study, 50% of the orchards were infected with Meloidogyne spp. It was determined that one orchard from each of Bartın and Zonguldak were infected with M. incognita, one orchard from Zonguldak with M. hapla and one orchard in Düzce with both M. incognita and M. hapla. It was determined that the population density in the orchards ranged between 31-252 J2/100 g soil and the highest population density was found as 252 J2/100 g soil in august in Yanaz Village of Bartın.

Keywords

Batı Karadeniz Bölgesi Kivi Bahçelerinde Kök-Ur Nematodu (Meloidogyne spp.) Yaygınlığı ve Yoğunluğu

Öz

Bu çalışmada kök-ur nematodlarının Batı Karadeniz Bölgesi kivi bahçelerindeki yaygınlık ve yoğunluklarının belirlenmesi amaçlanmıştır. Bu amaçla 2015 ve 2016 yıllarında Düzce (2), Zonguldak (3) ve Bartın (3) illerinde sekiz kivi bahçesinden kök ve toprak örnekleri alınmıştır. DNA ikinci dönem larvalardan ekstrakte edilmiş, ClearDetections firmasına ait M. tropical spp., M. hapla ve M. enterelobii real-time primerleri ve Meloidogyne spp. üniversal MF/MR primerleri moleküler teşhislerde kullanılmıştır. Çalışma sonucunda, bahçelerden %50’sinin Meloidogyne spp. ile bulaşık olduğu ve Bartın ve Zonguldak illerinden birer bahçenin M. incognita, Zonguldak ilinden bir bahçenin M. hapla ve Düzce ilinde bir bahçenin ise hem M. incognita hem de M. hapla ile bulaşık olduğu saptanmıştır. Bahçelerdeki popülasyon yoğunluğunun 31-252 J2/100 g toprak arasında değiştiği ve en yüksek popülasyon yoğunluğunun Bartın ili Yanaz Köyünde ağustos ayında 252 J2/100 g toprak olduğu belirlenmiştir.

Anahtar Kelimeler

Supporting Institution

Tarımsal Araştırmalar ve Politikalar Genel Müdürülüğü

Project Number

TAGEM-BS-13/08-09/01-22 (4)

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Details

Primary Language

Turkish

Subjects

Agricultural, Veterinary and Food Sciences

Journal Section

Research Article

Authors

Emre Evlice ^*
0000-0001-6402-0287
Türkiye

Esengül Özdemir
0000-0002-8274-282X
Türkiye

Publication Date

October 31, 2021

Submission Date

December 24, 2020

Acceptance Date

February 11, 2021

Published in Issue

Year 2021 Volume: 24 Number: 5

DOI

https://doi.org/10.18016/ksutarimdoga.vi.846406

IZ

https://izlik.org/JA24RZ49EG

Cite

RIS / Bibtex

APA

Evlice, E., & Özdemir, E. (2021). Batı Karadeniz Bölgesi Kivi Bahçelerinde Kök-Ur Nematodu (Meloidogyne spp.) Yaygınlığı ve Yoğunluğu. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 24(5), 1093-1099. https://doi.org/10.18016/ksutarimdoga.vi.846406

Cited By

Samsun İli (Türkiye) kivi bahçelerindeki kök-ur nematodlarının yaygınlığı ve meyve verimine etkileri

Turkish Journal of Entomology

https://doi.org/10.16970/entoted.1092654