Effect of Rosa pimpinellifolia Extract on Human Tumor Cells Proliferation

Elif Ayazoglu Demir; Selim Demir; Nihal Türkmen; İbrahim Turan

doi:10.18016/ksutarimdoga.vi.848137

TR EN

Rosa pimpinellifolia Ekstraktının İnsan Tümör Hücrelerinin Çoğalmasına Etkisi

Abstract

Rosa cinsi Rosaceae familyasının bir üyesi olup, Avrupa, Asya, Orta Doğu ve Kuzey Amerika'da dağılım gösteren 100'den fazla tür içerir. Rosa türleri, soğuk algınlığı, enfeksiyonlar ve çeşitli iltihaplı hastalıkların geleneksel tedavisinde uzun süredir kullanılmaktadır. Çeşitli çalışmalar bu cinsin antioksidan, antidiyabetik, anti-inflamatuar, renoprotektif ve antikanser aktivitelerini bildirmesine rağmen, Rosa pimpinellifolia'nın insan kanser hücreleri üzerindeki sitotoksik etkisini araştıran bir çalışma bulunmamaktadır. Bu çalışmanın amacı, R. pimpinellifolia'nın antioksidan özelliklerini ve insan kolon (WiDr), karaciğer (HepG2) ve akciğer (A549) kanser hücreleri üzerindeki sitotoksik etkisini normal bir fibroblast (BJ) hücre hattına kıyasla değerlendirmektir. Ekstraktın antioksidan özellikleri kolorimetrik yöntemler kullanılarak belirlenirken, sitotoksik etkisi ise MTT testi kullanılarak değerlendirildi. Ekstraktın toplam fenolik içeriği ve indirgeme gücü değerleri g örnek başına sırasıyla 16.4±0.4 mg gallik asit eşdeğeri ve 34.3±2.4 mg troloks eşdeğeri olarak hesaplandı. Ekstrakt BJ hücrelerine kıyasla üç kanser hücresi hattı üzerinde doza bağımlı bir şekilde seçici sitotoksik etki sergilemesine rağmen, en yüksek seçicilik indeks değeri (2.7 kat) WiDr hücre hattı için elde edildi. Sonuç olarak, bu çalışma R. pimpinellifolia'nın kanser hücreleri üzerindeki sitotoksik etkisini gösteren ilk çalışmadır. Bu sitotoksik etkinin moleküler mekanizmasını göstermek ve in vivo çalışmaların yapılması gelecekteki klinik çalışmalar için yol gösterici olabilir.

Keywords

Effect of Rosa pimpinellifolia Extract on Human Tumor Cells Proliferation

Abstract

The genus Rosa belongs to the Rosaceae family and contains more than 100 species which are distributed in Europe, Asia, the Middle East and North America. Rosa species have long been used in traditional treatment of cold, infections and various inflammatory diseases. Although various studies have reported antioxidant, antidiabetic, anti-inflammatory, renoprotective and anticancer activities of this genus, no previous studies have investigated the cytotoxic effect of Rosa pimpinellifolia on human cancer cells. The aim of this study was to evaluate the antioxidant properties of R. pimpinellifolia and its cytotoxic effect against human colon (WiDr), liver (HepG2) and lung (A549) cancer cells compared with a normal fibroblast (BJ) cell line. The antioxidant properties of extract was determined using colorimetric methods, while the cytotoxic effect of extract was evaluated using MTT assay. The total phenolic content and reducing power values of extract were calculated as 16.4±0.4 mg gallic acid equivalent and 34.3±2.4 mg trolox equivalent per g sample, respectively. Although the extract exhibits selective cytotoxic effect on three cancer cell lines in a dose-dependent manner compared to BJ cells, the highest selectivity index value (2.7-fold) was obtained for the WiDr cell line. In conclusion, this is the first study to demonstrate the cytotoxic effect of R. pimpinellifolia on cancer cells. To demonstrate the molecular mechanism of this cytotoxic effect and to carry out in vivo studies may be a guide for future clinical studies.

Keywords

References

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Elif Ayazoglu Demir
0000-0001-9027-7633
Türkiye

Selim Demir ^*
0000-0002-1863-6280
Türkiye

Nihal Türkmen
0000-0002-8913-8692
Türkiye

İbrahim Turan
0000-0003-3400-5494
Türkiye

Publication Date

December 31, 2021

Submission Date

December 28, 2020

Acceptance Date

March 25, 2021

Published in Issue

Year 2021 Volume: 24 Number: 6

DOI

https://doi.org/10.18016/ksutarimdoga.vi.848137

IZ

https://izlik.org/JA76XT52WN

Cite

RIS / Bibtex

APA

Ayazoglu Demir, E., Demir, S., Türkmen, N., & Turan, İ. (2021). Effect of Rosa pimpinellifolia Extract on Human Tumor Cells Proliferation. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 24(6), 1170-1176. https://doi.org/10.18016/ksutarimdoga.vi.848137

AMA

1.Ayazoglu Demir E, Demir S, Türkmen N, Turan İ. Effect of Rosa pimpinellifolia Extract on Human Tumor Cells Proliferation. KSU J. Agric Nat. 2021;24(6):1170-1176. doi:10.18016/ksutarimdoga.vi.848137

Chicago

Ayazoglu Demir, Elif, Selim Demir, Nihal Türkmen, and İbrahim Turan. 2021. “Effect of Rosa Pimpinellifolia Extract on Human Tumor Cells Proliferation”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi 24 (6): 1170-76. https://doi.org/10.18016/ksutarimdoga.vi.848137.

EndNote

Ayazoglu Demir E, Demir S, Türkmen N, Turan İ (December 1, 2021) Effect of Rosa pimpinellifolia Extract on Human Tumor Cells Proliferation. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 24 6 1170–1176.

IEEE

[1]E. Ayazoglu Demir, S. Demir, N. Türkmen, and İ. Turan, “Effect of Rosa pimpinellifolia Extract on Human Tumor Cells Proliferation”, KSU J. Agric Nat., vol. 24, no. 6, pp. 1170–1176, Dec. 2021, doi: 10.18016/ksutarimdoga.vi.848137.

ISNAD

Ayazoglu Demir, Elif - Demir, Selim - Türkmen, Nihal - Turan, İbrahim. “Effect of Rosa Pimpinellifolia Extract on Human Tumor Cells Proliferation”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 24/6 (December 1, 2021): 1170-1176. https://doi.org/10.18016/ksutarimdoga.vi.848137.

JAMA

1.Ayazoglu Demir E, Demir S, Türkmen N, Turan İ. Effect of Rosa pimpinellifolia Extract on Human Tumor Cells Proliferation. KSU J. Agric Nat. 2021;24:1170–1176.

MLA

Ayazoglu Demir, Elif, et al. “Effect of Rosa Pimpinellifolia Extract on Human Tumor Cells Proliferation”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, vol. 24, no. 6, Dec. 2021, pp. 1170-6, doi:10.18016/ksutarimdoga.vi.848137.

Vancouver

1.Elif Ayazoglu Demir, Selim Demir, Nihal Türkmen, İbrahim Turan. Effect of Rosa pimpinellifolia Extract on Human Tumor Cells Proliferation. KSU J. Agric Nat. 2021 Dec. 1;24(6):1170-6. doi:10.18016/ksutarimdoga.vi.848137

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