The Chemosensitizing Effect of Portulaca oleracea Methanol Extract on Human Colorectal and Lung Cancer Cells Against Irinotecan

Mehmet Kadir Erdoğan

doi:10.18016/ksutarimdoga.vi.848187

TR EN

Portulaca oleracea Metanol Ekstraktının İnsan Kolorektal ve Akciğer Kanseri Hücrelerini Irinotekana Karşı Kemohassaslaştırıcı Etkisi

Öz

Kanser büyük bir sağlık sorunudur ve son yıllarda dünya çapında büyük zararlara neden olmaktadır. Kanser tedavisinde kullanılan kemoterapötiklerin ciddi yan etkileri, doğal ürünleri değerli kılmaktadır. Kemoterapi ilaçları ile bitkisel ürünlerin birlikte kullanılması, yan etkilerin azaltılması ve daha etkili bir tedavinin ortaya çıkarılmasında önemli bir yaklaşımdır. Bu çalışmada, tek başına ve Irinotekan ile kombinasyon halinde Portulaca oleracea'dan metanol ekstraktının (POM) etkileri, insan kolorektal karsinoma hücre hattı HCT-116 ve insan akciğer adenokarsinom hücre hattı A549'a karşı araştırılmıştır. Antiproliferatif / anti-koloni oluşumu etkileri, WST-1 deneyi ve kloni oluşumu deneyi ile belirlendi. Hücre ölümü tespiti Elisa testi tetiklenen apoptozu göstermek için kullanıldı, ve apoptotik ve hayatta kalma yollarının önemli modülatörleri olan mTOR, Akt ve PTEN genlerinin mRNA ekspresyonunu değerlendirmek için kantitatif gerçek zamanlı polimeraz zincir reaksiyonu (qRT-PCR) yapıldı. Artan POM konsantrasyonları (0-1000 mg mL-1) ile 72 saatlik tedavinin doza bağlı olarak HCT116 ve A549 hücrelerinin canlılığını azalttığı belirlendi. Ayrıca, POM ve Irinotekan, her iki hücre hattında da sinerjik etkiler sergiledi. POM ve Irinotekan kombinasyonu, HCT116 ve A549 hücrelerinin koloni oluşturma kapasitesini sırasıyla % 84 ve % 79 oranında baskıladı. POM ayrıca HCT-116 ve A549 karsinom hücrelerinde mTOR, Akt ve PTEN gen ekspresyonlarını hem tek başına hem de Irinotekan ile kombinasyon halinde değiştirdi. Bu çalışma, P. oleracea'nın fitoterapötik etkisinin daha yoğun olarak araştırılması gerektiğini göstermektedir.

Anahtar Kelimeler

The Chemosensitizing Effect of Portulaca oleracea Methanol Extract on Human Colorectal and Lung Cancer Cells Against Irinotecan

Abstract

Cancer is a major health problem and it has been causing great damage in recent years worldwide. Serious side effects of chemotherapeutics used in cancer treatment make natural products valuable. Combined use of chemotherapy drugs and herbal products is an important approach in reducing side effects and revealing a more effective treatment. In this study, the effects of methanolic extract from Portulaca oleracea (POM) alone and in combination with Irinotecan were investigated against human colorectal carcinoma cell line HCT-116 and human lung adenocarcinoma cell line A549. The antiproliferative/anti-colony formation effects were determined using WST-1 assay and clonogenic assay. Cell death detection Elisa assay was employed to exhibite the triggered apoptosis, and quantitative real time polimerase chain reaction (qRT-PCR) was performed to evaluate the mRNA expression of mTOR, Akt and PTEN genes, which are significant modulators of apoptotic and survival pathways. It was determined that 72 h treatment with increasing concentrations of POM (0-1000 mg mL-1) decreased the viability of HCT116 and A549 cells in a dose-dependent manner. Also, POM and Irinotecan exhibited synergistic effects in both cell lines. The combination of POM and Irinotecan suppressed the colony forming capacity of HCT116 and A549 cells by 84% and 79%, respectively. POM also altered mTOR, Akt and PTEN gene expressions in HCT-116 and A549 carcinoma cells, both alone and in combination with Irinotecan. This study shows that the phytotherapeutic effect of P. oleracea needs to be studied more intensively.

Keywords

Thanks

The author would like to thank Mr. Yakup Yapar from Faculty of Arts and Sciences, Department of Biology, Bingol University for his kindly efforts in the identification of the plant samples and in the creation of herbarium records.

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Mehmet Kadir Erdoğan ^*
0000-0002-1579-5737
Türkiye

Publication Date

December 31, 2021

Submission Date

January 6, 2021

Acceptance Date

April 1, 2021

Published in Issue

Year 2021 Volume: 24 Number: 6

DOI

https://doi.org/10.18016/ksutarimdoga.vi.848187

IZ

https://izlik.org/JA58UG79MR

Cite

RIS / Bibtex

APA

Erdoğan, M. K. (2021). The Chemosensitizing Effect of Portulaca oleracea Methanol Extract on Human Colorectal and Lung Cancer Cells Against Irinotecan. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 24(6), 1187-1195. https://doi.org/10.18016/ksutarimdoga.vi.848187

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