LC-MS/MS Analysis, Antioxidant Properties and Inhibition Effect on Some Important Metabolic Enzymes of Nicotiana rustica L

Adem Necip; Mesut Işık; Abdussamat Güzel; Kasım Takım; Feyza Kaygısız

doi:10.18016/ksutarimdoga.vi.856514

TR EN

Nicotiana rustica L’nin Bazı Önemli Metabolik Enzimleri Üzerindeki İnhibisyon Etkisi, LC-MS / MS Analizi, Antioksidan Özellikleri

Öz

Nitrik oksit, hidrokarbonlar, aldehidler, fenollar, kinon ve semikinon radikalleri gibi pek çok kimyasal maddeyi bünyesinde barındıran ve doğrudan ya da dolaylı olarak oksijen kaynaklı serbest radikal oluşumuna yol açan tütün keşfedilmesinden günümüze kadar geçen zaman içinde yaygın olarak kullanılmaktadır. Bu yüzden dolayı Nicotiana rustica L.’nin fenolik içeriğinin, antioksidan/oksidan özelliklerinin bilinmesi ve önemli metabolik enzim olan Paraoksonaz (PON 1) ve Asetilkolinesteraz (AChE) üzerine etki çalışmaları önem arzetmektedir. Çalışmada Nicotiana rustica L.’nin su ve methanol ektresinin, PON 1 ve AChE enzim aktivitesi üzerine etkisine bakılmış. Nicotiana rustica Linn‘in su ektresi ceviz ile karıştırılmış maraş otu, (MPWW) ve methanol ektresi meşe ile karıştırılmış maraş otu (MPNR), ceviz ile karıştırılmış maraş otu (MPWW) ve bakır kazanlarında nemlendirilmiş maraş otu (MPCC) AChE üzerine inhibisyon etkisi (IC50 değerleri 0.104-0.203 mg ml -1 aralığında) gösterirken su ektresi MPCC ve methanol ektresi MPNR, MPWW ve MPCC ise PON 1 üzerine inhibisyon etkisi göstermiştir (IC50 değerleri 0.013-0. 25 mg ml -1 ). En yüksek radikal giderme aktivitesini MPNR su ektresi % 26 oranında DPPH radikal giderme aktivitesi gösterirken, MPCC su ektresi % 13 oranında ABTS radikal giderme aktivitesi göstermiştir.

Anahtar Kelimeler

LC-MS/MS Analysis, Antioxidant Properties and Inhibition Effect on Some Important Metabolic Enzymes of Nicotiana rustica L

Abstract

Being widely used since its discovery, tobacco contains many chemical substances including nitric oxide, hydrocarbons, aldehydes, phenols, quinone and semiquinone radicals, and causes the formation of oxygen-induced free radicals directly or indirectly. Therefore, it is important to know the oxidant/antioxidant properties, phenolic content of Nicotiana rustica Linn., and the effect studies on Paraoxonase (PON 1) and Acetylcholinesterase (AChE) which are important metabolic enzymes. In the study, the effect of extracts (water and methanol) of Nicotiana rustica Linn on PON 1 and AChE enzyme activity was examined. Water extract of Nicotiana rustica Linn Maras Powder with walnut, (MPWW) and methanol extract Maras Powder with Nicotiana rustica, (MPNR), Maras Powder with walnut, (MPWW) and Maras Powder with copper cauldron, (MPCC) had an inhibitory effect on AChE (IC50 values in the range of 0.104-0.203 mg ml-1). Also water extract MPCC and methanol extract MPNR, MPWW and MPCC showed an inhibition effect on PON 1 (IC50 values 0.013-0.225 mg ml -1). MPNR water extract showed the highest activity with 26% DPPH radical scavenging activity. MPCC water extract showed a 13% ABTS radical scavenging activity.

Keywords

Supporting Institution

Harran Unıversty

Project Number

1906

Thanks

This study was supported by the Scientific Research and Project Coordination Unit of Harran University. We would like to thank Harran University for its contribution.

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Adem Necip ^*
0000-0002-2092-7829
Türkiye

Mesut Işık
0000-0002-4677-8104
Türkiye

Abdussamat Güzel
0000-0001-7810-4510
Türkiye

Kasım Takım
0000-0003-4631-1982
Türkiye

Feyza Kaygısız
0000-0002-2899-2289
Türkiye

Publication Date

October 31, 2021

Submission Date

January 8, 2021

Acceptance Date

February 18, 2021

Published in Issue

Year 2021 Volume: 24 Number: 5

DOI

https://doi.org/10.18016/ksutarimdoga.vi.856514

IZ

https://izlik.org/JA28BS24UC

Cite

RIS / Bibtex

APA

Necip, A., Işık, M., Güzel, A., Takım, K., & Kaygısız, F. (2021). LC-MS/MS Analysis, Antioxidant Properties and Inhibition Effect on Some Important Metabolic Enzymes of Nicotiana rustica L. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 24(5), 930-938. https://doi.org/10.18016/ksutarimdoga.vi.856514