Comparative analyses of antioxidant, cytotoxic, and anti-inflammatory activities of different Cladonia species and determination of fumarprotocetraric acid amounts

Zekiye Kocakaya; Mustafa Kocakaya; Gökçe Şeker Karatoprak

doi:10.18016/ksutarimdoga.vi.868927

EN TR

Comparative analyses of antioxidant, cytotoxic, and anti-inflammatory activities of different Cladonia species and determination of fumarprotocetraric acid amounts

Abstract

In traditional medicine, Cladonia lichens are used for several disorders like hypertension, headaches, dizziness, cough, and menstrual disorders. The study aimed to determine the biological activities of 70% methanol extracts of nine Cladonia species from Turkey. The chemical composition was analyzed via spectrophotometric and chromatographic (HPLC) techniques. DPPH● (1,1-diphenyl-2-picrylhydrazyl) and ABTS+● (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging activities, inhibition of β-carotene/linoleic acid co-oxidation, and cytotoxicity on A549 and COLO205 cell lines were studied. Anti-inflammatory activity was assessed on the Raw 264.7 mouse macrophage cell line by determining nitric oxide (NO) levels. Among the studied Cladonia species Cladonia firma was found the most active extract in ABTS+● test system (2.58±0.07 mmol/L/Trolox) and also in the cytotoxicity and anti-inflammatory experiments. Cladonia pocillum, Cladonia rangiformis, and Cladonia foliaceae were also found to be active. Fumarprotocetraric acid amounts are between 1.89 -23.82 mg/gextract and fumarprotocetraric acid content did not show linearity with biological activities. It has been proved with this study that lichens traditionally used in treatment have as much biological activity as plants and can be evaluated in pharmaceuticals and cosmetics.

Keywords

Farklı Cladonia türlerinin antioksidan, sitotoksik ve antienflamatuvar aktivitelerinin karşılaştırmalı analizleri ve fumarprotosetrarik asit miktarlarının belirlenmesi

Öz

Geleneksel tıpta Cladonia likenleri, hipertansiyon, baş ağrısı, baş dönmesi, öksürük ve adet bozuklukları gibi çeşitli rahatsızlıklarda kullanılır. Çalışmada, Türkiye'den 9 Cladonia türünün % 70 metanol ekstrelerinin biyolojik aktivitelerinin belirlemesi amaçlandı. Kimyasal bileşimi, spektrofotometrik ve kromatografik (HPLC) tekniklerle analiz edildi. Ekstrelerin DPPH● (1,1-difenil-2-pikrilhidrazil) ve ABTS+● (2,2'-azino-bis (3-etilbenzotiyazolin-6-sülfonik asit) radikal süpürücü aktiviteleri, β-karoten/linoleik asit birlikte oksidasyonunun inhibisyonu ve A549 ve COLO205 hücre hatlarında sitotoksisiteleri çalışıldı. Antienflamatuvar aktivite, Raw 264.7 fare makrofaj hücre hattında nitrik oksit (NO) seviyelerinin belirlenmesiyle değerlendirildi. Çalışılan Cladonia türleri arasında Cladonia firma, ABTS+●test sisteminde (2.58 ± 0.07 mmol/L/Troloks) ve ayrıca sitotoksisite ve antienflamatuvar deneylerinde en aktif ekstre olarak belirlendi. Cladonia pocillum, Cladonia rangiformis ve Cladonia foliaceae'nin de aktif olduğu bulundu. Fumarprotosetrarik asit miktarları 1.89-23.82 mg/gekstre arasında olup fumarprotosetrarik asit içeriği biyolojik aktivitelerle doğrusallık göstermedi. Geleneksel olarak tedavide kullanılan likenlerin bitkiler kadar biyolojik aktiviteye sahip olduğu ve ilaç ve kozmetikte değerlendirilebileceği bu çalışma ile kanıtlanmıştır.

Anahtar Kelimeler

Supporting Institution

Bozok Üniversitesi

Project Number

6602b BMYO/17-121

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Zekiye Kocakaya
0000-0001-5248-0462
Türkiye

Mustafa Kocakaya
0000-0003-2306-8094
Türkiye

Gökçe Şeker Karatoprak ^*
0000-0001-5829-6914
Türkiye

Publication Date

December 31, 2021

Submission Date

January 26, 2021

Acceptance Date

March 3, 2021

Published in Issue

Year 2021 Volume: 24 Number: 6

DOI

https://doi.org/10.18016/ksutarimdoga.vi.868927

IZ

https://izlik.org/JA44TJ84JA

Cite

RIS / Bibtex

APA

Kocakaya, Z., Kocakaya, M., & Şeker Karatoprak, G. (2021). Comparative analyses of antioxidant, cytotoxic, and anti-inflammatory activities of different Cladonia species and determination of fumarprotocetraric acid amounts. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 24(6), 1196-1207. https://doi.org/10.18016/ksutarimdoga.vi.868927

Comparative analyses of antioxidant, cytotoxic, and anti-inflammatory activities of different Cladonia species and determination of fumarprotocetraric acid amounts

Comparative analyses of antioxidant, cytotoxic, and anti-inflammatory activities of different Cladonia species and determination of fumarprotocetraric acid amounts

Abstract

Keywords

Farklı Cladonia türlerinin antioksidan, sitotoksik ve antienflamatuvar aktivitelerinin karşılaştırmalı analizleri ve fumarprotosetrarik asit miktarlarının belirlenmesi

Öz

Anahtar Kelimeler

Supporting Institution

Project Number

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

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Determination of antimicrobial and anti-MRSA activities of Cladonia foliacea extracts