Efficacies of Entomopathogenic Fungi from Metarhizium, Beauveria and Isaria on German cockroach, Blattella germanica (L.) (Blattaria: Blattellidae)

Mehmet Kubilay Er; Hasan Tunaz; Ali Işıkber

doi:10.18016/ksutarimdoga.vi.881220

TR EN

Metarhizium, Beauveria ve Isaria’ya Bağlı Entomopatojen Fungusların Alman Hamamböceğine, Blattella germanica (L.) (Blattaria: Blattellidae), Etkinlikleri

Öz

Blattella germanica önemli bir zararlı olup insanların patojen ve parazitlerini taşıyabilmekte, alerjik reaksiyonlara ve yiyecek kontaminasyonlarına neden olabilmektedir. Mücadelesinde bugün için kullanılan kimyasallarla ilişkili riskler ve problemlerden dolayı alternatif yaklaşım olarak entomopatojen fungusların kullanımı araştırılmaktadır. Bu çalışmada, toprak örneklerinden izole edilmiş yedi ve entomopatojen fungus kültürlerinden beş entomopatojen fungus izolatı B. germanica’ya karşı test edilmiştir. Bu funguslar Beauveria bassiana (3 izolat), Metarhizium anisopliae (4 izolat), Isaria tenuipes (1 izolat), I. fumosorosea (3 izolat), ve I. farinosa (1 izolat) türlerinde yer almaktadır. Tüm izolatlara ait konidi süspansiyonları 1x107 konidi ml-1 konsantrasyonunda her tekerrürde on böceğe uygulanmıştır. Testler 26±2oC, %65±5 nispi nemde karanlıkta üç tekerrürlü olarak yürütülmüştür. B. germanica’ ya karşı etkili bulunan sekiz izolat 14 günde %60.0 ve %96.7 arasında değişen ölümlere neden olmuştur. Sonuçlar Metarhizium izolatlarının en yüksek etkinliğe (%83.3-95.8) sahip olduğunu, bunu Beauveria izolatlarının (%70.8-79.2) izlediğini ve en düşük etkinliğe Isaria izolatlarının (%50.0-70.8) sahip olduğunu işaret etmektedir. Bu izolatlar aynı türden raporlanmış önceki izolatlar kadar virülent olup daha yüksek etkinlik değerleri elde edilmiştir. M. anisopliae’ nın en etkili iki izolatı (S8-2 ve S11-6) hamamböceğine karşı mücadele etmeni olarak geliştirilmek için üzerinde çalışılmaya değer mikrobiyal mücadele etmenleri olarak tespit edilmiştir.

Anahtar Kelimeler

Efficacies of Entomopathogenic Fungi from Metarhizium, Beauveria and Isaria on German cockroach, Blattella germanica (L.) (Blattaria: Blattellidae)

Abstract

Blattella germanica is an important pest and able to transmit various pathogens and parasites of human, cause allergic reactions and food contamination. Due to risks and problems related to currently employed chemicals for their control, use of entomopathogenic fungi as alternative control approach has been under investigation. In this study, seven entomopathogenic fungi isolated from soil samples and five fungal isolates obtained from entomopathogenic fungal cultures have been tested against B. germanica. The fungi belong to the species Beauveria bassiana (3 isolates), Metarhizium anisopliae (4 isolates), Isaria tenuipes (1 isolate), I. fumosorosea (3 isolates), and I. farinosa (1 isolate). Conidial suspensions of all twelve isolates were applied at 1x107 conidia ml-1 concentration to ten insects in each replication. Tests were carried out at 26±2oC, 65±5% relative humidity in darkness with three replications. Eight isolates were found effective on B. germanica with mortalities varying between 60.0% and 96.7% in 14 days. The results indicated that Metarhizium isolates had the highest efficacies (83.3-95.8%) followed by Beauveria isolates (70.8-79.2%) and the isolates of Isaria had the lowest efficacies (50.0-70.8%). These isolates were as effective as the previously reported isolates of the same species with higher efficacy values. The most effective two isolates of M. anisopliae (S8-2 and S11-6) appear to be worth investigating further for developing a microbial control agent against cockroaches.

Keywords

Supporting Institution

TÜBİTAK

Project Number

110O159

Thanks

Thanks to Dr. Richard A. Humber for providing entomopathogenic fungal cultures from USDA ARS Collection of Entomopathogenic Fungal Culture Collection

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Details

Primary Language

English

Subjects

Agricultural, Veterinary and Food Sciences

Journal Section

Research Article

Authors

Mehmet Kubilay Er ^*
0000-0003-1568-8656
Türkiye

Hasan Tunaz
0000-0003-4942-6056
Türkiye

Ali Işıkber
0000-0003-1213-3532
Türkiye

Publication Date

February 28, 2022

Submission Date

February 16, 2021

Acceptance Date

March 29, 2021

Published in Issue

Year 2022 Volume: 25 Number: 1

DOI

https://doi.org/10.18016/ksutarimdoga.vi.881220

IZ

https://izlik.org/JA22ZC88YP

Cite

RIS / Bibtex

APA

Er, M. K., Tunaz, H., & Işıkber, A. (2022). Efficacies of Entomopathogenic Fungi from Metarhizium, Beauveria and Isaria on German cockroach, Blattella germanica (L.) (Blattaria: Blattellidae). Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 25(1), 105-112. https://doi.org/10.18016/ksutarimdoga.vi.881220