The Effects of Different Concentrations of Foliar Applied Chitosan, Iron Oxide and Chitosan-Coated Iron Oxide Nanoparticles on the Secondary Metabolites of Hypericum triquetrifolium Turra. During Full Bloom

Ayşe Bal; Hasan Çetin Özen; Bilsen Tural; Erdal Ertaş

doi:10.18016/ksutarimdoga.vi.882856

EN TR

The Effects of Different Concentrations of Foliar Applied Chitosan, Iron Oxide and Chitosan-Coated Iron Oxide Nanoparticles on the Secondary Metabolites of Hypericum triquetrifolium Turra. During Full Bloom

Abstract

Hypericum triquetrifolium Turra. (Hypericaceae) is one of the important medicinal plants. This herb is used in Turkish folk medicine for its antidepressant, anthelmintic and antiseptic effects. Hypericum extracts have an important commercial value in the pharmaceutical industry. Therefore, studies to increase the amount of secondary metabolites it contains are becoming widespread. Elicitors are biological and non-biological factors that can affect the synthesis of secondary metabolites in plants. In recent years, nanoelicitors have been used to increase the amount of active ingredients. In this study, to stimulate the synthesis of biologically active secondary compounds of H. triquetrifolium; chitosan, iron oxide and chitosan-coated iron oxide nanoparticles in concentrations of 0 (control), 50, 75, 100 and 150 ppm were sprayed on the leaves during full bloom. LC-MS/MS analysis showed that application of 100 and 150 ppm chitosan nanoparticles increased the amount of flavonol (hyperocyte and quercitrin) and naphthodianthrons (pseudohypericin and hypericin) in H. triquetrifolium. 50 ppm iron oxide nanoparticle hyperocyte, quercitrin and pseudohypericin; 75 and 100 ppm iron oxide nanoparticles increased the amount of hyperocyte, quercitrin and hyperforin. The 150 ppm iron oxide nanoparticle resulted in an increase in all compounds except hypericin. In this study, iron oxide nanoparticles coated with chitosan were also used as elicitors to improve the chemical and biological properties of iron oxide. In this series, iron oxide nanoparticle coated with 100 ppm chitosan was effective and increased the amounts of quercitrine, kaempferol and pseudohypericin. The concentration of 75 ppm of this group was effective on quercitrin.

Keywords

Tam Çiçeklenme Döneminde Yapraktan Uygulanan Kitosan, Demir Oksit ve Kitosanla Kaplanmış Demir Oksit Nanopartiküllerinin Farklı Konsantrasyonlarının Hypericum triquetrifolium Turra.’nın İkincil Metabolitleri Üzerine Etkileri

Öz

Hypericum triquetrifolium Turra. (Hypericaceae) önemli tıbbi bitkilerden biridir. Bu bitki Türk halk tıbbında antidepresan, antelmintik ve antiseptik etkileri nedeniyle kullanılmaktadır. Hypericum özütleri ilaç endüstrisinde önemli bir ticari değere sahiptir. Bu nedenle içerdiği ikincil metabolitlerin miktarını artırmaya yönelik çalışmalar yaygınlaşmaktadır. Elisitörler, bitkilerde ikincil metabolitlerin sentezini etkileyebilen biyolojik ve biyolojik olmayan faktörlerdir. Son yıllarda, aktif bileşen miktarını artırmak için nanoelisitörler kullanılmaktadır. Bu çalışmada, H. triquetrifolium'un biyolojik aktif sekonder bileşiklerinin sentezini uyarmak için; tam çiçeklenme döneminde yaprakların üzerine 0 (kontrol), 50, 75, 100 ve 150 ppm konsantrasyonlarında kitosan, demir oksit ve kitosanla kaplanmış demir oksit nanopartikülleri püskürtülmüştür. LC-MS/MS analizi, 100 ve 150 ppm’lik kitosan nanopartikülleri uygulanmasının, H. triquetrifolium'da flavonol (hiperosit ve kuersitrin) ve naftodiantronların (psödohiperisin ve hiperisin) miktarını artırdığını göstermiştir. 50 ppm demir oksit nanopartikülü hiperosit, kuersitrin ve psödohiperisin; 75 ve 100 ppm'lik demir oksit nanopartikülleri ise hiperosit, kuersitrin ve hiperforin miktarlarını artırmıştır. 150 ppm'lik demir oksit nanopartikülü, hiperisin dışındaki tüm bileşiklerde artış sağlamıştır. Bu çalışmada, demir oksidin kimyasal ve biyolojik özelliklerini iyileştirmek için kitosanla kaplanmış demir oksit nanopartikülleri de elisitör olarak kullanılmıştır. Bu seride, 100 ppm'lik kitosanla kaplanmış demir oksit nanopartikülü etkili olmuş ve kuersitrin, kaempferol ve psödohiperisin miktarlarını arttırmıştır. Bu grubun 75 ppm'lik konsantrasyonu kuersitrin üzerinde etkili olmuştur.

Anahtar Kelimeler

Supporting Institution

Dicle Üniversitesi, Bilimsel Araştırmalar Proje (DUBAP)Koordinatörlüğü tarafından destek sağlanmıştır.

Project Number

project no: FEN.18.013

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Ayşe Bal ^*
0000-0002-3181-7772
Türkiye

Hasan Çetin Özen
0000-0001-6670-6469
Türkiye

Bilsen Tural
0000-0001-7555-2481
Türkiye

Erdal Ertaş
0000-0002-0325-1257
Türkiye

Publication Date

August 31, 2022

Submission Date

February 18, 2021

Acceptance Date

September 3, 2021

Published in Issue

Year 2022 Volume: 25 Number: 4

DOI

https://doi.org/10.18016/ksutarimdoga.vi.882856

IZ

https://izlik.org/JA54EC29GU

Cite

RIS / Bibtex

APA

Bal, A., Özen, H. Ç., Tural, B., & Ertaş, E. (2022). The Effects of Different Concentrations of Foliar Applied Chitosan, Iron Oxide and Chitosan-Coated Iron Oxide Nanoparticles on the Secondary Metabolites of Hypericum triquetrifolium Turra. During Full Bloom. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 25(4), 811-818. https://doi.org/10.18016/ksutarimdoga.vi.882856

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