Investigation of the Biological Activities of Alcea calvertii

Mahmoud Abudayyak; Şeyda Kanbolat; Reyyan Ergene; Şeyma Batur; Rezzan Aliyazıcıoğlu

doi:10.18016/ksutarimdoga.vi.890659

TR EN

Alcea calvertii'nin Biyolojik Aktivitelerinin İncelenmesi

Abstract

Bitkiler ve bitkisel ilaçlar soğuk algınlığı, enfeksiyonlar ve kanser gibi farklı hastalıkların tedavisinde kullanılmaktadır. Alcea calvertii, Malvaceae familyasına ait çok yıllık otsu bir bitkidir. Anadolu ve Akdeniz bölgesinde yayılan bu bitki etnobotanik açısından önemli bir yere sahiptir. Bu çalışmada, Alcea calvertii bitkisinin topraküstü kısımlarının metanol, su, kloroform ve etil asetat ekstrelerinin antioksidan aktiviteleri ile bu ekstrelerin sitotoksik potansiyellerinin incelenmesi amaçlanmıştır. Bu kapsamda, Alcea calvertii’nin antioksidan aktivitesi dört farklı yöntem [toplam fenolik içeriği (TPC), ferrik indirgeyici antioksidan gücü (FRAP), bakır indirgeyici antioksidan kapasitesi (CUPRAC) ve 2,2-difenilpikrilhidrazil (DPPH) radikal süpürme aktivitesini] ile tespit edilmiştir. İnsan akciğer kanseri hücre hattındaki (A549) sitotoksik potansiyeli ise MTT testi ile belirlenmiştir. En yüksek antioksidan aktivitenin metanol ekstresinde olduğu ve artan ekstre konsantrasyonu ile antioksidan aktivitenin arttığı gözlenmiştir; TPC değerlerinin 62,5- 414,6 GAE µg mL-1, FRAP değerlerinin 115,7- 1321,4 µM Trolox eşdeğeri g-1, CUPRAC değerlerinin 177,1 - 1321,4 µM Trolox eşdeğeri g-1, DPPH tespitinde IC50 değerlerinin 0,0089- 3,5370 mg mL-1 arasında hesaplanmıştır. Ekstreler konsantrasyona bağlı olarak hücre ölümüne neden olmuştur ve IC50 değerleri 36,8-62,64 µg mL-1 arasında bulunmuştur. Bu sonuçlar umut verici olup daha kapsamlı çalışmalar ile incelenmesinin önemli olduğu düşünülmektedir.

Keywords

Investigation of the Biological Activities of Alcea calvertii

Abstract

Herbs are widely used in the treatment of diseases as colds, infections, and cancer. In this work, we evaluate Alcea calvertii, which is a perennial herbaceous plant belonging to the Malvaceae family. It spreads in Anatolia and Mediterranean region and has important uses in terms of ethnobotany. In this study, it was aimed to evaluate the cytotoxic potentials and to investigate the antioxidant activities of methanol, water, chloroform, and ethyl acetate extracts of the aerial parts of Alcea calvertii. For that, the antioxidant activity of Alcea calvertii was determined by four different methods [total phenolic content (TPC), ferric reducing antioxidant power (FRAP), copper reducing antioxidant capacity (CUPRAC) and 2,2-diphenylpicrylhydrazil (DPPH) radical scavenging activity. The cytotoxicity potential of extracts was assessed in the human lung cancer cell line (A549) by MTT assay. It was observed that the highest antioxidant activity was in the methanol extract and the antioxidant activity increased with increasing extract concentration; The TPC values were between 62.5 - 414.6 GAE µg mL-1, the FRAP values were between 115.7 - 1321.4 µM Trolox equivalent g-1, CUPRAC values were between 177.1 - 1321.4 µM Trolox equivalent g-1, and IC50 values in DPPH determination were between 0.0089 - 3.5370 mg mL-1. The extracts caused cytotoxicity in a concentration dependent manner, the IC50 values were calculated to be between 36.8 - 62.64 µg mL-1. It is concluded that Alcea calvertii could be an important herb in developing new drugs.

Keywords

Supporting Institution

TÜBİTAK 2209A

Project Number

1919B011800561

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Mahmoud Abudayyak ^*
0000-0003-2286-4777
Türkiye

Şeyda Kanbolat
0000-0001-7261-7067
Türkiye

Reyyan Ergene
0000-0003-4985-9812
Türkiye

Şeyma Batur
0000-0003-0934-0223
Türkiye

Rezzan Aliyazıcıoğlu
0000-0003-0143-8795
Türkiye

Publication Date

October 31, 2022

Submission Date

March 13, 2021

Acceptance Date

October 28, 2021

Published in Issue

Year 2022 Volume: 25 Number: 5

DOI

https://doi.org/10.18016/ksutarimdoga.vi.890659

IZ

https://izlik.org/JA99SS67PF

Cite

RIS / Bibtex

APA

Abudayyak, M., Kanbolat, Ş., Ergene, R., Batur, Ş., & Aliyazıcıoğlu, R. (2022). Investigation of the Biological Activities of Alcea calvertii. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 25(5), 955-964. https://doi.org/10.18016/ksutarimdoga.vi.890659

AMA

1.Abudayyak M, Kanbolat Ş, Ergene R, Batur Ş, Aliyazıcıoğlu R. Investigation of the Biological Activities of Alcea calvertii. KSU J. Agric Nat. 2022;25(5):955-964. doi:10.18016/ksutarimdoga.vi.890659

Chicago

Abudayyak, Mahmoud, Şeyda Kanbolat, Reyyan Ergene, Şeyma Batur, and Rezzan Aliyazıcıoğlu. 2022. “Investigation of the Biological Activities of Alcea Calvertii”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi 25 (5): 955-64. https://doi.org/10.18016/ksutarimdoga.vi.890659.

EndNote

Abudayyak M, Kanbolat Ş, Ergene R, Batur Ş, Aliyazıcıoğlu R (October 1, 2022) Investigation of the Biological Activities of Alcea calvertii. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 25 5 955–964.

IEEE

[1]M. Abudayyak, Ş. Kanbolat, R. Ergene, Ş. Batur, and R. Aliyazıcıoğlu, “Investigation of the Biological Activities of Alcea calvertii”, KSU J. Agric Nat., vol. 25, no. 5, pp. 955–964, Oct. 2022, doi: 10.18016/ksutarimdoga.vi.890659.

ISNAD

Abudayyak, Mahmoud - Kanbolat, Şeyda - Ergene, Reyyan - Batur, Şeyma - Aliyazıcıoğlu, Rezzan. “Investigation of the Biological Activities of Alcea Calvertii”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 25/5 (October 1, 2022): 955-964. https://doi.org/10.18016/ksutarimdoga.vi.890659.

JAMA

1.Abudayyak M, Kanbolat Ş, Ergene R, Batur Ş, Aliyazıcıoğlu R. Investigation of the Biological Activities of Alcea calvertii. KSU J. Agric Nat. 2022;25:955–964.

MLA

Abudayyak, Mahmoud, et al. “Investigation of the Biological Activities of Alcea Calvertii”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, vol. 25, no. 5, Oct. 2022, pp. 955-64, doi:10.18016/ksutarimdoga.vi.890659.

Vancouver

1.Mahmoud Abudayyak, Şeyda Kanbolat, Reyyan Ergene, Şeyma Batur, Rezzan Aliyazıcıoğlu. Investigation of the Biological Activities of Alcea calvertii. KSU J. Agric Nat. 2022 Oct. 1;25(5):955-64. doi:10.18016/ksutarimdoga.vi.890659

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