Light-Mediated Biosynthesis of Phenylpropanoid Metabolites in Cell Suspension Cultures of Turkish Yarrow (Achillea gypsicola Hub. Mor.)

Muhammed Akif Açıkgöz; Ebru Batı Ay; Ahmet Aygün; Şevket Metin Kara

doi:10.18016/ksutarimdoga.vi.926160

TR EN

Türk Civanperçemi (Achillea gypsicola Hub. Mor.) Hücre Süspansiyon Kültürlerinde Fenilpropanoid Metabolitlerin Işık Aracılığıyla Biosentezi

Öz

Türk civanperçemi (Achillea gypsicola Hub. Mor.) tıbbi açıdan değerli metabolitler içerir. Işık, bitkilerde başta fotosentez olmak üzere fenipropanoid metabolitlerin birikimi gibi birçok fizyolojik olayda kilit bir rol oynar. Bu çalışmanın amacı, ışığın Türk civanperçemi hücre süspansiyon kültüründe fenilpropanoid metabolitlerin biyosentezi ve biyokütle artışı üzerindeki etkilerini belirlemektir. Tohumlardan elde edilen in vitro bitkicikler eksplant kaynağı olarak kullanılmış ve gövde segmentinden türetilen kallus dokularından hücre kültürleri oluşturulmuştur. Daha sonra, 0.5 mg/L BA (benzilaminopurin)+0.5 mg/L NAA (naftalen asetik asit) ile desteklenmiş B5 ortamında muhafaza edien 8 günlük hücre kültürleri, aydınlık ve karanlık ortamlarda inkübe edilmiştir. Toplamda beş kez (1, 2, 3, 6 ve 12. gün) hücre hasadı yapılmıştır. Kamfor miktarı gas chromatography-mass spectrometry (GC-MS) ile belirlenmiştir. Diğer parametreler spektrofotometrik yöntem kullanılarak hesaplanmıştır. Toplam fenolik miktarı karanlık ve aydınlık koşullarda örnek alma zamanına bağlı olarak artış göstermiştir. Fenolik madde birikimi başlangıç kültürüne göre %29.25 artmıştır. Işık uygulaması kamfor miktarını 1.12 kat, flavanol miktarını 4.9 kat ve flavonol miktarını 0.5 kat artırmıştır. Bu sonuçlar bize A. gypsicola hücre kültürlerinde ışığın hücre büyümesi ve sekonder metabolit birikimindeki etkisini açıkça ortaya koymuştur.

Anahtar Kelimeler

Light-Mediated Biosynthesis of Phenylpropanoid Metabolites in Cell Suspension Cultures of Turkish Yarrow (Achillea gypsicola Hub. Mor.)

Abstract

Turkish yarrow (Achillea gypsicola Hub. Mor.) contains medicinally valuable bioactive metabolites. Light plays a key role in many physiological events in plants such as the accumulation of phenylpropanoid metabolites, particularly photosynthesis. The aim of this study was to determine the effects of light on the biosynthesis of phenylpropanoid metabolites and biomass growth in Turkish yarrow cell suspension cultures. In vitro plantlets obtained from seeds were used as explant sources and cell cultures of A. gypsicola were established using stem segment-derived callus tissues. Then, 8-day-old cell cultures kept in a B5 medium supplemented with 0.5 mg/L BA (benzylaminopurine)+0.5 mg/L NAA (naphthalene acetic acid) were incubated in light and dark environments. Cells were harvested five times (1st, 2nd, 3rd, 6th, and 12th day) in total. The amount of camphor was determined by gas chromatography-mass spectrometry (GC–MS). Other parameters were calculated using the spectrophotometric method. The total phenolic amount increased depending on the sampling time in dark and light conditions. Phenolic accumulation was increased by 29.25% compared to the initial culture. Also, light application increased the amount of camphor 1.12 times, the amount of flavanol 4.9 times, and the amount of flavonol 0.5 times. These results clearly demonstrate the effect of light on cell growth and phenylpropanoid metabolite accumulation in A. gypsicola cell cultures.

Keywords

Supporting Institution

TÜBİTAK

Project Number

1001- 114O564.

Thanks

The authors are grateful to the Scientific and Technological Research Council of Turkey (TÜBİTAK) for providing financial support for this work under Project No. 1001- 114O564. This study is a part of Dr Muhammed Akif Açıkgöz’s doctorate thesis.

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Details

Primary Language

English

Subjects

Agricultural, Veterinary and Food Sciences

Journal Section

Research Article

Authors

Muhammed Akif Açıkgöz
0000-0003-2436-5605
Türkiye

Ebru Batı Ay ^*
0000-0002-9210-6907
Türkiye

Ahmet Aygün
0000-0001-7745-3380
Türkiye

Şevket Metin Kara
0000-0001-7755-1394
Türkiye

Publication Date

December 30, 2022

Submission Date

April 22, 2021

Acceptance Date

December 7, 2021

Published in Issue

Year 2022 Volume: 25 Number: 6

DOI

https://doi.org/10.18016/ksutarimdoga.vi.926160

IZ

https://izlik.org/JA42DX79HZ

Cite

RIS / Bibtex

APA

Açıkgöz, M. A., Batı Ay, E., Aygün, A., & Kara, Ş. M. (2022). Light-Mediated Biosynthesis of Phenylpropanoid Metabolites in Cell Suspension Cultures of Turkish Yarrow (Achillea gypsicola Hub. Mor.). Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 25(6), 1234-1242. https://doi.org/10.18016/ksutarimdoga.vi.926160

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