Research Article

Determination of Lethal Effects of Some Entomopathogenic Fungi on Different Biological Stages of Leptinotarsa decemlineata Say

Volume: 25 Number: 5 October 31, 2022
TR EN

Determination of Lethal Effects of Some Entomopathogenic Fungi on Different Biological Stages of Leptinotarsa decemlineata Say

Abstract

The lethal effects of Beauveria bassiana BIM-001 (1x106 spore mL-1, 1x107 spore mL-1, 1x108 spore mL-1), Fusarium subglutinans 12A (obtained from cotton aphid in Adana-Karataş) and the commercial preparation of 1.5% B. bassiana strain Bb-1 (250 mL da; 1x108 spore mL-1) (Nostalgist SL) on Leptinotarsa decemlineata were investigated. The isolates were applied to the eggs, larvae (1st, 2nd, 3rd, and 4th larval stages) and adults of L. decemlineata by spraying in laboratory conditions (25 ± 1°C, 60 ± 10% relative humidity and 16:8 [L: D] photoperiod). The observations to determine the lethal effect of isolates were performed up to 9 days for the eggs and larvae, and 21 days for the adults of L. decemlineata following the first spore suspension application. In the experiments conducted on the larval stages, it was determined that the mortality rates due to all entomopathogenic fungus isolates and at different concentrations were significantly different from the mortality rate of the control. The effect of all spore concentrations of B. bassiana BIM-001 was found significantly higher than other treatments and the mortality rates were 100% for all larval stages (P ˂0.05). In addition, the mortality rates were between 4 and 16% for adults across all treatment groups except for the control. Moreover, it has been determined that each three spore concentrations of B. bassiana BIM-001 also suppressed the egg hatching of L.decemlineata by 55 to 60%

Keywords

Supporting Institution

Isparta Uygulamalı Bilimler Üniversitesi. BİLİMSEL ARAŞTIRMA PROJELERİ KOORDİNASYON BİRİMİ

Project Number

2019-YL1-0010

Thanks

The authors thank the management unit of Scientific research projects of Isparta University of Applied Sciences, Turkey (Project number: 2019-YL1-0010). This article is produced from the first author's 'Determination of pathogenic effects of some entomopathogenic fungus on different biological stages of Leptinotarsa decemlineata Say’ titled master thesis.

References

  1. Alves SB, Rossi LS, Lopes RB, Tamai MA, Pereira RM 2002. Beauveria bassiana yeast phase on agar medium and its pathogenicity against Diatraea saccharalis (Lepidoptera: Crambidae) and Tetranychus urticae (Acari: Tetranychidae). Journal of Invertebrate Pathology, 81(2): 70-77. https://doi.org/10.1016/S0022-2011(02)00147-7
  2. Arıcı ŞE, Gülmez İ, Demirekin H, Zahmekıran H, Karaca İ 2012. Entomopatojen Fusarium subglutinans’ın bakla yaprakbiti, Aphis fabae Scopoli (Hemiptera: Aphididae) üzerine etkisi. Türkiye Biyolojik Mücadele Dergisi, 3(1): 89-96.
  3. Atak U 1973. Trakya Bölgesinde Patates Böceği (Leptinotarsa decemlineata Say.)’nin morfolojisi, Bio-ekolojisi ve Savaş Metotları Üzerinde Araştırmalar. TC Tarım Bakanlığı Zirai Mücadele ve Zirai Karantina Genel Müdürlüğü Yayınları, Teknik Bülten, 6, pp. 63.
  4. Demirözer O, Arıcı ŞE, Sevinç MS, Karaca İ 2010. Fusarium subglutinans (Wollenw. & Reinking) (Hypocreales: Nectriaceae)’ın Chilocorus nigritus (Fabricius) (Coleoptera: Coccinellidae) üzerindeki patolojik etkisinin belirlenmesine yönelik ön çalışma. Türkiye Biyolojik Mücadele Dergisi, 1(2): 151-155.
  5. Demirözer O, Uzun A, Arıcı ŞE, Gep İ, Bakay R 2015. Effects of Fusarium subglutinans on Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae). 5. Entomopatojenler ve Mikrobiyal Mücadele Kongresi, Ankara.
  6. Demirözer O, Uzun A, Arıcı ŞE, Gep İ, Bakay R 2016. Insecticidal effect of Fusarium subglutinans on Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae). Hellenic Plant Protection Journal, 9(2): 66-72.
  7. Erdoğan P, Toros S 2010. Azadirachta indica A. Juss ekstraktlarının Patates böceği [Leptinotarsa decemlineata Say (Col: Chrysomelidae)] gelişimine etkisi. Bitki Koruma Bülteni, 50(2): 73-88.
  8. Erkılıç L, Pala H, Başpınar N, Biçer Y 1999. Doğu Akdeniz Bölgesi’nde bazı yaprakbiti türlerinde entomopatojen fungusların belirlenmesi. Türkiye 4. Biyolojik Mücadele Kongresi Bildirileri, Adana, pp. 623-632.

Details

Primary Language

English

Subjects

Agricultural, Veterinary and Food Sciences

Journal Section

Research Article

Publication Date

October 31, 2022

Submission Date

April 27, 2021

Acceptance Date

October 2, 2021

Published in Issue

Year 2022 Volume: 25 Number: 5

APA
Uysal, H. M., Demirözer, O., & Uzun, A. (2022). Determination of Lethal Effects of Some Entomopathogenic Fungi on Different Biological Stages of Leptinotarsa decemlineata Say. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 25(5), 1036-1041. https://doi.org/10.18016/ksutarimdoga.vi.928869

Cited By


International Peer Reviewed Journal
Free submission and publication
Published 6 times a year



88x31.png


KSU Journal of Agriculture and Nature

e-ISSN: 2619-9149