Effects of Melatonin on Tomato Infected with Root-knot Nematode Meloidogyne incognita

Zeliha Gökbayrak; Çiğdem Gözel

doi:10.18016/ksutarimdoga.vi.938501

EN TR

Effects of Melatonin on Tomato Infected with Root-knot Nematode Meloidogyne incognita

Abstract

This study was conducted in 2020 to investigate the influence of melatonin (10, 50 and 100 µM) given in three methods (immersion, irrigation, and foliar spraying) on some physiological aspects of tomato seedlings inoculated with root-knot nematode Meloidogyne incognita [(Kofoid and White) Chitwood]. The seedlings were inoculated with 1000 second-stage juveniles of M. incognita. Dualex® optic sensor was used to in situ measure total chlorophylls, flavonols, anthocyanins contents and nitrogen balance index (NBI). Results indicated that while no significant effects were observed on chlorophyll content, melatonin ameliorated the adverse effects of M. incognita on chlorophyll depending on the concentration and mode of application. Flavonols were at the highest in the irrigated plants and the lowest in the immersed ones. NBI was affected by the method the melatonin was applied, and immersing boosted it while irrigation caused a significant decrease. Irrigation resulted in significantly higher anthocyanins compared to the other two methods. One observation was that applying melatonin in the low and medium concentrations to the soil containing nematode increased the anthocyanin content of the plant. Melatonin merits a value in developing a response against the nematode but needs further elucidation.

Keywords

Melatoninin Kök-ur Nematodu Meloidogyne incognita ile İnfekteli Domates Üzerindeki Etkileri

Öz

Bu çalışma 2020 yılında üç yöntem ile (daldırma, sulama ve spreyleme) verilen melatoninin (10, 50 ve 100 µM) kök-ur nematodu Meloidogyne incognita [(Kofoid and White) Chitwood] uygulanan domates fidelerinin bazı fizyolojik yönleri üzerindeki etkisini araştırmak için yürütülmüştür. Fidelere 1000 adet ikinci dönem M. incognita larvası inoküle edilmiştir. Toplam klorofil, flavonol ve antosiyanin içerikleri ile azot balans indeksini (NBI) ölçmek için Dualex® klorofilmetre kullanılmıştır. Sonuçlar, klorofil içeriği üzerinde önemli bir etki olmadığını, melatoninin konsantrasyona ve yönteme bağlı olarak M. incognita’nın klorofil üzerindeki olumsuz etkilerini iyileştirdiğini göstermiştir. Flavonoller, sulama ile verilen bitkilerde en yüksek, daldırma ile verilenlerde ise en düşük olmuştur. NBI, melatoninin uygulanma yönteminden etkilenmiş, daldırma ile artış, sulama ile ciddi bir düşüş göstermiştir. Sulama, diğer iki yönteme kıyasla önemli ölçüde yüksek antosiyaninlere sebep olmuştur. Nematodlu bir toprağa düşük ve orta konsantrasyonlarda melatonin uygulanmasının antosiyaninleri arttırdığı gözlemlenmiştir. Melatonin, nematodlara karşı tepki geliştirmede önemli olup daha fazla çalışmaya ihtiyaç duyulmaktadır.

Anahtar Kelimeler

Supporting Institution

TÜBİTAK

Project Number

119O660

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Details

Primary Language

English

Subjects

Agricultural, Veterinary and Food Sciences

Journal Section

Research Article

Authors

Zeliha Gökbayrak ^*
0000-0002-0012-9782
Türkiye

Çiğdem Gözel
0000-0002-0742-7205
Türkiye

Publication Date

August 31, 2022

Submission Date

May 18, 2021

Acceptance Date

August 31, 2021

Published in Issue

Year 2022 Volume: 25 Number: 4

DOI

https://doi.org/10.18016/ksutarimdoga.vi.938501

IZ

https://izlik.org/JA88UY68KB

Cite

RIS / Bibtex

APA

Gökbayrak, Z., & Gözel, Ç. (2022). Effects of Melatonin on Tomato Infected with Root-knot Nematode Meloidogyne incognita. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 25(4), 698-705. https://doi.org/10.18016/ksutarimdoga.vi.938501

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