Biosynthesis of Silver Nanoparticles Using White Propolis Extract as a Reduction Agent and Optimized by Box-Behnken Design

Nazan Gökşen Tosun; Özlem Kaplan

doi:10.18016/ksutarimdoga.vi.945859

TR EN

Gümüş Nanopartiküllerin Beyaz Propolis Ekstresi Kullanılarak Biyosentezi ve Box-Behnken Yöntemi ile Optimizasyonu

Abstract

Bu çalışmada gümüş nanopartiküllerin biyosentezinin Box-Behnken yöntemi ile optimize edilmesi amaçlandı. Beyaz propolis özütü, bir indirgeme ve stabilize edici ajan olarak kullanıldı. Beyaz propolis özüt çözeltisi kullanılarak sentezlenen gümüş nanopartiküller, mikrodalganın gücü, zaman, AgNO3 (gümüş nitrat) konsantrasyonu ve beyaz propolis özüt çözeltisinin hacminin AgNO3 hacmine oranı gibi farklı faktörlerin etkisi dikkate alınarak Box-Behnken yöntemi ile optimize edildi. Matematiksel modellemede ikinci dereceden polinom modeli kullanıldı ve bağımsız değişken-yanıt ilişkisini belirlemek için yanıt yüzey analizi yapıldı. Optimum koşullar 10 mM AgNO3 konsantrasyonu, oran: 0.3, 150-watt mikrodalga gücü ve 35 saniye olarak belirlendi. Optimize edilmiş gümüş nanopartiküller, FTIR (Kızılötesi) spektroskopisi, UV-VIS (Ultraviyole görünür bölge) spektrofotometri ve DLS (Dinamik ışık saçılımı) kullanılarak karakterize edildi. Ek olarak, optimize edilmiş gümüş nanopartiküllerin antibakteriyel aktivitesi Staphylococcus aureus (S. aureus), Klebsiella pneumoniae (K. pneumoniae), Pseudomonas aeruginosa (P. aeruginosa) ve Enterococcus faecalis (E. faecalis) suşlarına karşı test edildi. Gümüş nanopartiküllerin bakteriler üzerinde propolis ekstraktına göre daha etkili olduğu gözlendi.

Keywords

Biosynthesis of Silver Nanoparticles Using White Propolis Extract as a Reduction Agent and Optimized by Box-Behnken Design

Abstract

In this study, it was aimed to optimize the biosynthesis of silver nanoparticles with a Box-Behnken design. The white propolis extract was utilized as the reduction and stabilizing agent. The synthesized silver nanoparticles using white propolis extract solution were optimized by Box-Behnken design considering the effect of certain independent variables such as microwave power, time, and concentration of AgNO3 (silver nitrate). A quadratic polynomial model was used in mathematical modeling and response surface analysis was performed to determine the independent variable-response relationship. The optimum synthesis conditions were determined as 10 mM of AgNO3 concentration, 0.3 of VExt/VAg, 150 watts of microwave power, and 35 seconds. The optimized silver nanoparticles were characterized using FTIR (Fourier Infrared) spectroscopy, UV-VIS (Ultraviolent visible) spectrophotometry, and DLS (Dynamic Light Scattering). In addition, the antibacterial activity of the optimized silver nanoparticles was tested against Staphylococcus aureus (S. aureus), Klebsiella pneumonia (K. pneumoniae), Pseudomonas aeruginosa (P. aeruginosa), and Enterococcus faecalis (E. faecalis) strains. It was observed that synthesized silver nanoparticles had higher antibacterial activity compared to propolis extract.

Keywords

References

Antoine TE, Hadigal SR, Yakoub AM, Mishra Y K, Bhattacharya P, Haddad C 2016. Intravaginal zinc oxide tetrapod nanoparticles as novel immunoprotective agents against genital herpes. The Journal of Immunology, 196(11): 4566–4575.
Bansal M, Bansal A, Sharma M, Kanwar P, June M 2020. Green synthesis of gold and silver nanoparticles. Research Journal of Pharmaceutical Biological and Chemical Sciences, 6(1710): 1710–1716.
Barbosa VT, Souza JKC, Alvino V, Meneghetti MR, Florez-Rodriguez PP, Moreira RE 2019. Biogenic synthesis of silver nanoparticles using Brazilian propolis. Biotechnology Progress, 35(6): e2888.
Cho KH, Park JE, Osaka T, Park SG 2005. The study of antimicrobial activity and preservative effects of nanosilver ingredient. Electrochimica Acta, 51(5): 956–960.
Corciovă A, Mircea C, Burlec AF, Cioancă O, Tuchiluş C, Fifere A 2019. Antioxidant, antimicrobial, and photocatalytic activities of silver nanoparticles obtained by bee propolis extract assisted biosynthesis. Farmacia, 67(3): 482–489.
Dada AO, Inyinbor AA, Idu EI, Bello OM, Oluyori AP, Adelani-Akande TA 2018. Effect of operational parameters, characterization and antibacterial studies of green synthesis of silver nanoparticles using Tithonia diversifolia. PeerJ, 6: 1–17.
Dhiman M, Sharma L, Singh A, Sharma MM 2021. Biogenic fabrication of silver nanoparticles using Sterculia urens Roxb. And assessment of their antimicrobial efficiency. Materials Today: Proceedings, 43(5): 3206-3210.
Garibo D, Borbón-Nuñez HA, de León JND 2020. Green synthesis of silver nanoparticles using Lysiloma acapulcensis exhibit high-antimicrobial activity. Scientific Reports, 10(1):12805.

Ghramh HA, Khan KA, Ibrahim EH, Ansari MJ 2019. Biogenic synthesis of silver nanoparticles using propolis extract, their characterization, and biological activities. Science of Advanced Materials, 11(6): 876–883.
Gökşen Tosun N, Kaplan Ö 2021. Optimization of the green synthesis of silver nanoparticles with Box-Behnken design: using Aloe vera plant extract as a reduction agent. Sakarya University Journal of Science, 25(1): 200– 211.
Hasnain MS, Siddiqui S, Rao S, Mohanty P, Jahan Ara T, Beg S 2016. QbD-driven development and validation of a bioanalytical LC-MS method for quantification of fluoxetine in human plasma. Journal of Chromatographic Science, 54(5): 736–743.
Joy Prabu H, Johnson I 2015. Plant-mediated biosynthesis and characterization of silver nanoparticles by leaf extracts of Tragia involucrata, Cymbopogon citronella, Solanum verbascifolium and Tylophora ovata. Karbala International Journal of Modern Science, 1(4): 237–246.
Kaplan Ö, Gökşen Tosun N 2021. Biosynthesis of silver nanoparticles from Teucrioside and investigation of its antibacterial activity. Cumhuriyet Science Journal, 42(1): 60–67.
Kaplan Ö, Gökşen Tosun N, Özgür A, Erden Tayhan S, Bilgin S, Türkekul İ, Gökce İ 2021. Microwave-assisted green synthesis of silver nanoparticles using crude extracts of Boletus edulis and Coriolus versicolor: characterization, anticancer, antimicrobial, and wound healing activities. Journal of Drug Delivery Science and Technology, 64: 102641.
Khadri H, Alzohairy M, Janardhan A, Kumar AP, Narasimha G 2013. Green synthesis of silver nanoparticles with high fungicidal activity from olive seed extract. Advances in Nanoparticles, 02(03): 241–246.
Khatoon A, Khan F, Ahmad N, Shaikh S, Rizvi SMD, Shakil S 2018. Silver nanoparticles from leaf extract of Mentha piperita: Eco-friendly synthesis and effect on acetylcholinesterase activity. Life Sciences, 209(May): 430–434.
Khorrami S, Zarrabi A, Khaleghi M, Danaei M, Mozafari MR 2018. Selective cytotoxicity of green synthesized silver nanoparticles against the MCF-7 tumor cell line and their enhanced antioxidant and antimicrobial properties. International Journal of Nanomedicine, 13: 8013–8024.
Kischkel B, Castilho PF de, de Oliveira KMP, Rezende PST, Bruschi ML, Svidzinski TIE, Negri M 2020. Silver nanoparticles stabilized with propolis show reduced toxicity and potential activity against fungal infections. Future Microbiology, 15(7): 521–539.
Kumar Nayak A, Saquib Hasnain M, Malakar J 2013. Development and optimization of hydroxyapatite-ofloxacin implants for possible bone delivery in osteomyelitis treatment. Current Drug Delivery, 10(2): 241–250.
Li D, Mathew B, Mao C 2012. Biotemplated synthesis of hollow double-layered core/shell titania/silica nanotubes under ambient conditions. Small, 8(23): 3691–3697.
Lin L, Wang W, Huang J, Li Q, Sun D, Yang X 2010. Nature factory of silver nanowires: Plant-mediated synthesis using broth of Cassia fistula leaf. Chemical Engineering Journal, 162(2): 852–858.
Malakar J, Das K, Nayak AK 2014. In situ cross-linked matrix tablets for sustained salbutamol sulfate release - formulation development by statistical optimization. Polymers in Medicine, 44(4):221-230.
Mohanpuria P, Rana NK, Yadav SK 2008. Biosynthesis of nanoparticles: technological concepts and future applications. Journal of Nanoparticle Research, 10(3): 507–517.
Nagai T, Inoue R, Inoue H, Suzuki N 2003. Preparation and antioxidant properties of water extract of propolis. Food Chemistry, 80(1): 29–33.
Nayak AK, Kalia S, Hasnain MS 2013. Optimization of aceclofenac-loaded pectinately (vinyl pyrrolidone) beads by response surface methodology. International Journal of Biological Macromolecules, (62): 194–202.
Padalia H, Moteriya P, Chanda S 2015. Green synthesis of silver nanoparticles from marigold flower and its synergistic antimicrobial potential. Arabian Journal of Chemistry, 8(5): 732–741.
Raghunandan D, Mahesh BD, Basavaraja S, Balaji SD, Manjunath SY, Venkataraman A 2011. Microwave-assisted rapid extracellular synthesis of stable bio-functionalized silver nanoparticles from guava (Psidium guajava) leaf extract. Journal of Nanoparticle Research, 13(5): 2021–2028.
Roy N, Barik A 2010. Green synthesis of silver nanoparticles from the unexploited weed resources. International Journal of Nanotechnology and Applications, 4(2): 95–101.
Yadav A, Gupta U, Sharma R 2021. Nano Drug Delivery Strategies for the Treatment of Cancers. Nano drug delivery strategies for the treatment of cancers. Academic Press.

Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Nazan Gökşen Tosun ^*
0000-0001-5269-1067
Türkiye

Özlem Kaplan
0000-0002-3052-4556
Türkiye

Publication Date

October 31, 2022

Submission Date

May 31, 2021

Acceptance Date

October 1, 2021

Published in Issue

Year 2022 Volume: 25 Number: 5

DOI

https://doi.org/10.18016/ksutarimdoga.vi.945859

IZ

https://izlik.org/JA52RA68FR

Cite

RIS / Bibtex

APA

Gökşen Tosun, N., & Kaplan, Ö. (2022). Biosynthesis of Silver Nanoparticles Using White Propolis Extract as a Reduction Agent and Optimized by Box-Behnken Design. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 25(5), 933-945. https://doi.org/10.18016/ksutarimdoga.vi.945859

AMA

1.Gökşen Tosun N, Kaplan Ö. Biosynthesis of Silver Nanoparticles Using White Propolis Extract as a Reduction Agent and Optimized by Box-Behnken Design. KSU J. Agric Nat. 2022;25(5):933-945. doi:10.18016/ksutarimdoga.vi.945859

Chicago

Gökşen Tosun, Nazan, and Özlem Kaplan. 2022. “Biosynthesis of Silver Nanoparticles Using White Propolis Extract As a Reduction Agent and Optimized by Box-Behnken Design”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi 25 (5): 933-45. https://doi.org/10.18016/ksutarimdoga.vi.945859.

EndNote

Gökşen Tosun N, Kaplan Ö (October 1, 2022) Biosynthesis of Silver Nanoparticles Using White Propolis Extract as a Reduction Agent and Optimized by Box-Behnken Design. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 25 5 933–945.

IEEE

[1]N. Gökşen Tosun and Ö. Kaplan, “Biosynthesis of Silver Nanoparticles Using White Propolis Extract as a Reduction Agent and Optimized by Box-Behnken Design”, KSU J. Agric Nat., vol. 25, no. 5, pp. 933–945, Oct. 2022, doi: 10.18016/ksutarimdoga.vi.945859.

ISNAD

Gökşen Tosun, Nazan - Kaplan, Özlem. “Biosynthesis of Silver Nanoparticles Using White Propolis Extract As a Reduction Agent and Optimized by Box-Behnken Design”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 25/5 (October 1, 2022): 933-945. https://doi.org/10.18016/ksutarimdoga.vi.945859.

JAMA

1.Gökşen Tosun N, Kaplan Ö. Biosynthesis of Silver Nanoparticles Using White Propolis Extract as a Reduction Agent and Optimized by Box-Behnken Design. KSU J. Agric Nat. 2022;25:933–945.

MLA

Gökşen Tosun, Nazan, and Özlem Kaplan. “Biosynthesis of Silver Nanoparticles Using White Propolis Extract As a Reduction Agent and Optimized by Box-Behnken Design”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, vol. 25, no. 5, Oct. 2022, pp. 933-45, doi:10.18016/ksutarimdoga.vi.945859.

Vancouver

1.Nazan Gökşen Tosun, Özlem Kaplan. Biosynthesis of Silver Nanoparticles Using White Propolis Extract as a Reduction Agent and Optimized by Box-Behnken Design. KSU J. Agric Nat. 2022 Oct. 1;25(5):933-45. doi:10.18016/ksutarimdoga.vi.945859

Cited By

Green Innovation and Synthesis of Honeybee Products-Mediated Nanoparticles: Potential Approaches and Wide Applications

Bioengineering

https://doi.org/10.3390/bioengineering11080829

Evaluation of Anticancer and Antimicrobial Potentials of Tarantula cubensis Venom (Theranekron® D6)

Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi

https://doi.org/10.18185/erzifbed.1463282