The effects of root lesion nematodes (Pratylenchus thornei) on rhizobium bacteria of chickpea plant

Tohid Behmand; Ibrahim Halil Elekcioğlu

doi:10.18016/ksutarimdoga.vi.956915

TR EN

Kök lezyon nematodlarının (Pratylenchus thornei) nohut bitkisinin rhizobium bakterileri üzerinde etkisi

Öz

Nohut (Cicer arietinum) en önemli baklagil bitkilerinden biridir ve insan beslenmesi için yüksek kaliteli protein sağlar. nohut gibi baklagil bitkileri, spesifik toprak bakterileriyle azot fiksasyonuna yönelik simbiyotik yetenekleri nedeniyle tarımsal açıdan önemlidir. Nohut gibi baklagiller büyümek için aktivitenin sağladığı nitrojene bağlıdır, ancak bu rizobium bakterileri nodül bitkilerinde daha az aktiviteye neden olan ve nodül sayısını azaltan bitki paraziti nematodlardan etkilenir. Kök lezyon nematodları (Pratylenchus thornei), dünyada tarımı ve büyüyen nohut tarlasını en önemli sınırlayıcı faktörlerden biri olarak tanımlanan yaygın ve ekonomik açıdan önemli zararlılardır. Bu çalışmada, bu nematodun rizobium (nodül) sayısı ve rhizobium bakteri aktivitesi üzerindeki etkileri, laboratuvar koşullarında Cicer türlerinin hem yabani hem de yerli genotiplerinde değerlendirilmiştir. Tüm nohut genotiplerini Mesorhizobium bakterileri ve Pratylenchus cinsinin bir türü (Pratylenchus thornei) ile bulaştırıldı. Sonuç, P. thornei'nin nodül sayısı ve rhizobium bakterilerinin aktivitesi üzerinde olumsuz bir etkisi olduğunu gösterdi. Nohutta nematod enfeksiyonu nodülasyonun azalmasına neden olmuştur. Nerdeyse, nematod ile enfekte olmuş bitki, enfekte olmayan bitkiye göre 4-8 arsı az nodül sayısı oluşturdu.

Anahtar Kelimeler

The effects of root lesion nematodes (Pratylenchus thornei) on rhizobium bacteria of chickpea plant

Abstract

Chickpea (Cicer arietinum) is one of the most significant legume crops and supply high-quality protein for human nutrition. legume crops such as chickpea are important agriculturally because of their symbiotic ability to nitrogen fixation with specific soil bacteria. Legumes like chickpea depend on nitrogen provided by the activity to grow, but these rhizobium bacteria are affected by plant-parasitic nematodes that cause less activity and decrease the number of nodules in the chickpea plant. The root-lesion nematodes (Pratylenchus thornei) are common and economically important pests described as one of the major limiting factors in agriculture and the growing chickpea field in the world. In this study, the effects of this nematode on the number of rhizobia (nodules) and rhizobium bacteria activity were assessed in both wild and domesticated accession of Cicer species under laboratory conditions. We inoculated all Cicer accession with the Mesorhizobium bacteria and with one species of the genus Pratylenchus (P. thornei). The result showed that P. thornei has a negative impact on the number of nodules and the activity of rhizobium bacteria. Nematode infection on chickpea caused decreased nodulation. Where, nematode infected plant formed 4-8 nodules/root and less nodule number than an uninfected plant.

Keywords

Supporting Institution

The Commonwealth Scientific and Industrial Research Organisation (CSIRI)

Project Number

CSR00185

References

Abdalla AS, Osman AG, Abdelgani ME, Rugheim AME 2011. Effects of biological and mineral fertilization on nodulation, nitrogen and phosphorus content and yield of chickpea (Cicer arietinum L.). J. Environ. Biol 5: 2886–2894. [Google Scholar]
Ballhorn DJ, Younginger BS, Kautz S 2014. An aboveground pathogen inhibits belowground rhizobia and arbuscular mycorrhizal fungi in Phaseolus vulgaris. BMC Plant Biol. 14:321. [PMC free article] [PubMed] [Google Scholar]
Behmand T, Kasapoglu Uludamar EB, Berger J, Elekcioglu İH 2019. Development of methodology for resistance screening of chickpea genotypes collected in Turkey to the root-lesion nematode, Pratylenchus thornei Sher & Allen, 1953 (Tylenchida: Pratylenchidae). Turk. J. Entomol 44 (1): 81-89
Bhuiyan MAH, Khanam D, Hossain MF, Ahmed MS 2008. Effect of Rhizobium inoculation on nodulation and yield of chickpea in calcareous soil. Bangladesh j. agric. res. 33: 549–554. [Crossref], [Google Scholar]
Burghardt LT , Guhlin J, Lan C, Junqi C, Sadowsky MJ, Stupar RM, Young ND, Tiffin P 2017. Transcriptomic Basis of the genome by genome variation in a legume‐rhizobia mutualism. Mol.Ecol. 26:6122–6135. 10.1111/mec.14285. [PubMed] [CrossRef] [Google Scholar]
Busby PE, Soman C, Wagner MR, Friesen ML, Kremer J, Bennett A, Morsy M, Eisen JA, Leach JE, Dangl JL 2017. Research priorities for harnessing plant microbiomes in sustainable agriculture. PLoS Biol. 15:1–14. [PMC free article] [PubMed] [Google Scholar]
Buttery B, Park SJ , van Berkum P 1997. Effects of common bean (Phaseolus vulgaris L.) cultivar and rhizobium strain on plant growth, seed yield, and nitrogen content. Canadian Journal of Plant Science 77: 347–351. [Crossref], [Web of Science ®], [Google Scholar]
Carter J M, Gardner W K, Gibson AH 1994. Improved growth and yield of faba beans (Vicia faba cv. Fiord) by inoculation with strains of Rhizobium leguminosarum biovar viciae in acid soils in southwest Victoria. Aust. J Agric Res. 1994; 45:613–623. [Google Scholar]

Castillo P, Vovlas N 2007. Pratylenchus (Nematoda: Pratylenchidae): Diagnosis, Biology, Pathogenicity, and Management. Nematology Monographs and Perspectives, Brill Leiden-Boston, The Netherlands-USA, 6: 529 pp.
Correa OS, Barneix AJ 1997. Cellular mechanisms of pH tolerance in Rhizobium loti. World .J. Microbiol Biotechnol 13:153–157. [Google Scholar]
Danso SKA, Hera C, Douka C (1987) Nitrogen fixation in soybean as influenced by cultivar and Rhizobium strain. Plant and Soil 99: 163–174. [Crossref], [Web of Science ®], [Google Scholar]
Dhandaydham M, Charles L, Zhu H, Starr JL, Huguet T, Cook DR, Prosperi JM, Opperman C 2008. Characterization of root‐knot nematode resistance in Medicago truncatula . J. Nematol 40:46–54. [PMC free article] [PubMed] [Google Scholar]
Elhadi EA, Elsheikh EAE 1999. Effect of Rhizobium inoculation and nitrogen fertilization on yield and protein content of six chickpea (Cicer arietinum L.) cultivars in marginal soils under irrigation. Nutrient Cycling in Agroecosystems 54: 57–63. [Crossref], [Web of Science ®], [Google Scholar]
FAO 2017. Food and Agriculture Organization of the United Nations Statistical Data. (Web page: www.fao.org/faostat/en) (Date accessed: 02.11.2017).
Friesen ML 2013. Microbially mediated plant functional traits. Mol. Microb. Ecol. Rhizosph 1:87–102. [Google Scholar]
Garcia J, Barker DG, Journet EP 2006. Seed storage and germination in Mathesius U., Journet E., and Sumner L., eds. Medicago truncatula Handbook. Noble Research Institute, Ardmore, OK. pp 1–9 [Google Scholar]
Goverse A, Smant G 2014. The activation and suppression of plant innate immunity by parasitic nematodes. Annual Review of Phytopathology 52:243–265. [PubMed] [Google Scholar]
Heath KD, Tiffin P 2009 Stabilizing mechanisms in a legume‐rhizobium mutualism. Evolution 63:652–662. [PubMed] [Google Scholar]
Hooper DJ 1986 Extraction of Free-Living Stages from Soil, 5-30. In: Laboratory Methods for Work with Plant and Soil Nematodes (Ed. J. F. Southey). Ministry of Agriculture, Fisheries, and Food Technology, Reference Book 402, Her Majesty’s Stationary Office, London, UK, 629 pp.
Hussey RS, Barker KR 1976. Influence of nematodes and light sources on growth and nodulation of soybean. Journal Nematology 8:49–52. [PMC free article] [PubMed] [Google Scholar]
Nicol JM, Vanstone VA 1993. Carrot piece and chickpea callus culture for Pratylenchus thornei and P. neglectus, 254-264. In: Proceedings of the Pratylenchus Workshop, 9th Biennial Conference of the Australasian Plant Pathology Society (8-9 July 1993, Hobart, Tasmania, Australia), 347 pp.
Rupela OP, Saxena MC 1987. Nodulation and nitrogen fixation in chickpea. In: The Chickpea,eds. M.C. Saxena and K.B. Singh,pp.191-206. Wallingford, UK: CABInternational. [Google Scholar]
Sasser JN, Freckman DW 1987. A world perspective on nematology: The role of the society,” in Vistas on Nematology, eds J. A. Veech and D. W. Dickson (Hyattsville, MD: Society of Nematologists), 7–14.
Sattar MA, Podder AK, Das ML, Shaikh MAQ , Danso SK A 1998. Evaluation of chickpea and groundnut for N2 fixation and yield in Bangladesh. In: Improving Yield and Nitrogen Fixation of Grain Legumes in the Tropics and Sub-Tropics of Asia, pp. 131–146. Vienna: International Atomic Energy Agency. [Google Scholar]
Shapira M 2016. Gut microbiotas and host evolution: scaling up symbiosis. Trends Ecol. Evol. 31:539–549. [PubMed] [Google Scholar]
Singh M, Sharma SB 1994. Temperature effects on development and reproduction of Heterodera cajani on pigeon pea. Journal Nematology 26:241-248.
Somasegaran P, Hoben HJ 1994. Handbook for Rhizobia: Methods in Legume-Rhizobium Technology. Springer, Heidelberg.
Strauss SY, Irwin RE 2004. Ecological and evolutionary consequences of multispecies plant‐animal interactions. Annu. Rev. Annual Review of Ecology, Evolution, and Systematics 35:435–466. [Google Scholar]
Sudupak A, Akkaya MS, Kence A 2002. Analysis of genetic relationships among perennial and annual Cicer species growing in Turkey using RAPD markers. Theoretical and Applied Genetics 105: 1220-1228.
Vovlas N, Castillo P, Troccoli A 1998. Histology of nodular tissue of three leguminous hosts infected by three root-knot nematode species. International journal of nematology 8, 105–110.
Wood C W, Pilkington BL, Vaidya P, Biel C, Stinchcombe JR 2018. Genetic conflict with a parasitic nematode disrupts the legume-rhizobia mutualism. Evolution Letters 37:453. doi: 10.1002/evl3.51. [PMC free article] [PubMed] [CrossRef] [Google Scholar]

Details

Primary Language

English

Subjects

Agricultural, Veterinary and Food Sciences

Journal Section

Research Article

Authors

Tohid Behmand ^*
0000-0001-7227-2484
Türkiye

Ibrahim Halil Elekcioğlu
0000-0003-0936-3759
Türkiye

Publication Date

June 30, 2022

Submission Date

June 24, 2021

Acceptance Date

October 2, 2021

Published in Issue

Year 2022 Volume: 25 Number: 3

DOI

https://doi.org/10.18016/ksutarimdoga.vi.956915

IZ

https://izlik.org/JA62BK48HM

Cite

RIS / Bibtex

APA

Behmand, T., & Elekcioğlu, I. H. (2022). The effects of root lesion nematodes (Pratylenchus thornei) on rhizobium bacteria of chickpea plant. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 25(3), 521-527. https://doi.org/10.18016/ksutarimdoga.vi.956915

Cited By

Soil Nematode Community Analysis of Four Chickpea Cultivated Areas in Aksaray, Türkiye

Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi

https://doi.org/10.18016/ksutarimdoga.vi.1577590