Evaluation of Antibacterial Effect of Honey on ESBL and Biofilm-Producing Enterobacterales

Seda Özdikmenli Tepeli; Burcu Kaya; Dilvin İpek

doi:10.18016/ksutarimdoga.vi.971261

TR EN

Balın GSBL ve Biyofilm Üreten Enterobacterales Üzerindeki Antibakteriyel Etkisinin Değerlendirilmesi

Öz

Mastitis, süt veriminin düşmesine ve ekonomik kayıplara neden olan bir meme bezi hastalığıdır. Mastitis bakteriyolojiktir; bu nedenle tedavilerinde genellikle antibiyotikler kullanılır. Mastitis tedavisinde antibiyotik kullanımının azaltılması için arı ürünleri, fitoterapi, uçucu yağlar gibi alternatif doğal tedavi yöntemleri değerlendirilmektedir. Bu çalışmada, distile su ve borik asit (%2) solventlerinde çözündürülen çiçek ve meşe balı örneklerinin ESBL ve biyofilm üreticisi Enterobacterales patojenleri üzerindeki in vitro antibakteriyel etkisi araştırıldı. Amaç bal/borik asit solüsyonunun büyükbaş göğüs yüzeylerinde doğal bir antiseptik solüsyon olarak mastitise karşı kullanılabilirliğini ortaya koymaktır. Borik asit ve distile su çözücülü bal çözeltileri arasında istatistiksel olarak anlamlı bir fark yoktur. Çiçek balı çözeltilerinde artan bal oranına göre antibakteriyel etkiler artmıştır. Ancak borik asit çözeltisinde çözülen meşe balının antibakteriyel etkinliği distile suda çözünenden daha yüksekti. Çiçek ve meşe balı örnekleri arasındaki istatistiksel ilişki analizi sonucunda çiçek balı örneklerinin antibakteriyel etkileri meşe balı örneklerine göre daha yüksek bulunmuştur (p<0.05) (R=0,825). Bu çalışma ile literatürde ilk defa bal ve borik asit ile mastitis tedavisine alternatif bir formülasyon geliştirilmiştir.

Anahtar Kelimeler

Evaluation of Antibacterial Effect of Honey on ESBL and Biofilm-Producing Enterobacterales

Abstract

Mastitis is a mammary gland inflammatory disease that causes milk yield reduction and economic losses. Mastitis is bacteriological and antibiotics are usually used for treatment. Alternative natural treatment methods such as bee products, phytotherapy, and essential oils were evaluated to reduce the use of antibiotics in the treatment of mastitis. In this study, the in vitro antibacterial effect of flower and oak honey samples dissolved in distilled water and boric acid (2%) on ESBL and biofilm-producing Enterobacterales pathogens was investigated. The aim was to establish the usability of honey/boric acid solution against mastitis as a natural antiseptic solution for bovine udder surfaces. Honey samples were studied by dissolving in distilled water and boric acid (2%) solvents. There was no significant statistical difference between honey solutions using distilled water and boric acid (p>0.05). Antibacterial effects were increased according to the increasing honey proportion in flower honey solutions. However, the antibacterial activity of oak honey dissolved in boric acid solution was higher than dissolved in distilled water. As a result of the statistical correlation analysis between flower and oak honey samples, antibacterial effects of flower honey samples were determined to be higher than oak honey samples (p<0.05) (R=0.825). An alternative formulation for mastitis treatment with honey and boric acid was developed for the first time in the literature.

Keywords

Thanks

We are thankful to Assoc. Prof. Dr. Nükhet N. Demirel Zorba and her laboratory members for their kind support about standard bacterial strains, Food Engineering Department, Çanakkale Onsekiz Mart University, Çanakkale, Turkey. This research received no grant from any funding agency/sector.

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Details

Primary Language

English

Subjects

Agricultural, Veterinary and Food Sciences

Journal Section

Research Article

Authors

Seda Özdikmenli Tepeli ^*
0000-0002-1613-5917
Türkiye

Burcu Kaya
0000-0003-1755-7705
Türkiye

Dilvin İpek
0000-0003-2946-5437
Türkiye

Publication Date

December 30, 2022

Submission Date

July 14, 2021

Acceptance Date

February 10, 2022

Published in Issue

Year 2022 Volume: 25 Number: Ek Sayı 1

DOI

https://doi.org/10.18016/ksutarimdoga.vi.971261

IZ

https://izlik.org/JA26ZG53XC

Cite

RIS / Bibtex

APA

Özdikmenli Tepeli, S., Kaya, B., & İpek, D. (2022). Evaluation of Antibacterial Effect of Honey on ESBL and Biofilm-Producing Enterobacterales. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 25(Ek Sayı 1), 54-64. https://doi.org/10.18016/ksutarimdoga.vi.971261

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