The Functions of Eicosanoids on Nodulation Reactions to Two Fungal Isolates In Adults of The German Cockroach, Blatella germanica (L.)
Abstract
One of the cellular defense reactions to
microbial
infections in insects is nodulation
reactions. In this study, the role of eicosanoids on nodulation
reactions to two fungal isolates (Beauveria
bassiana 1512 and Metarhizium anisopliae S8-2) in adults of the German
cockroach, Blatella germanica (L.) (Dictyoptera: Blattellidae) was tested. Treating adult of
German cockroach with Beauveria 1512
and Metarhizium S8-2 induced
nodulation reactions. Injecting adults
of B. germanica with eicosanoid
biosynthesis inhibitors, immediately before intrahemocoelic injections of the
fungal isolates reduced the nodulation response to fungal challenges.
Separate treatments with specific inhibitors including dexamethasone (a
phospholipase A2 inhibitor), indomethacin, naproxen, ibuprofen
(cyclooxygenase inhibitors), esculetin (a lipoxygenase inhibitor) and Phenidone
(dual cyclooxgenase/lipoxygenase inhibitor) also impaired the ability of B.
germanica to form
nodules in reaction to fungal challenge. These findings support the hypothesis
that eicosanoids has a role on nodulation reactions to the fungal infections in
adults of B. germanica.
References
- Dunn, P.E. 1986. Biochemical aspects of insect immunology. Annu Rev Entomol, 31: 321-339.
- Gillespie, J.P., Kanost, M.R., Trenczek, T. 1997. Biological mediators of insect immunity. Annual Review of Entomology, 42: 611-643.
- Miller, J.S., Nguyen, T., Stanley-Samuelson, D,W. 1994. Eicosanoids mediate insect nodulation responses to bacterial infections. Proceedings of the National Acaddemy of Sciences USA, 91: 12418-12422.
- Rosales, C. 2017. Cellular and Molecular Mechanisms of Insect Immunity. In: Shields,V.D.C. Ed. Insect Physiology and Ecology. InTeach Publicaiton. CCBY. Pp. 179-212.
- Russell, V., Dunn, P.E. 1996. Antibacterial proteins in the midgut of Manduca sexta during metamorphosis. Journal of Insect Physiolology, 42: 65-71.
- SAS Institute Inc. 1989. SAS/STATR User's Guide, Version 6, 4th Ed., vol 2. SAS Institute Inc., Cary, NC.
- Satyavathi, V.V., Minz, A., Nagaraju, J. 2014. Nodulation: An unexplored cellular defense mechanism in insects. Cellular Signalling, 26: 1753-1763.
- Stanley, D.W. 2000. Eicosanoids in Invertebrate Signal Transduction Systems. Princeton University Press, Princeton, NJ.
- Stanley, D.W., Kim, Y. 2014. Eicosanoid Signaling in Insects: from Discovery to Plant Protection. Plant Sciences, 33:20-63.
- Stanley, D.W., Miller, J.S. 2006. Eicosanoid actions in insect cellular immune functions. Entomologia Experimentalis et Applicata, 119: 1-13.
- Tunaz, H. 2009. Eicosanoids act in nodulation reactions to bacteria Serratia marcescens in hemipteran pest, Eurygaster Integriceps Turkish Journal of Biology 33: 301-309.
- Tunaz, H., Bengin, C., Er, M.K. 2008. Nodulation reaction to fungal infections in larvae of Leptinotarsa decemlineata(Say) (Coleoptera: Chrysomelidae) mediated by eicosanoids. Turkish Journal of Agricultural Forestry 32: 11-18.
- Tunaz, H. 2006. Influence of Eicosanoids in nodulation reactions against bacteria, Serratia marcescens in larvae of Leptinotarsa decemlineata. KSU Fen Mühendislik Dergisi, 9:159-163.
Alman Hamamböceği Blatella germanica (L.)'nın Erginlerinde İki Fungal İzolata Karşı Eikosanoidlerin Nodülasyon Reaksiyonları Üzerine Olan Fonksiyonu
Abstract
mikrobiyal enfeksiyonlara karşı hücresel savunma reaksiyonlarından biri
nodülasyon reaksiyonlarıdır. Bu çalışmada, Alman hamamböceği, Blatella
germanica (L.) (Dictyoptera:
Blattellidae)'nın
erginlerinde eikosanoidlerin, iki fungal izolata (Beauveria bassiana'nın 1512
ve Metarhizium anisopliae'nin S8-2) nodülasyon reaksiyonları üzerindeki rolü
test edilmiştir. Ergin böcekler 1512 ve S8-2 ile enfekte edildiğinde, böcekler
bu enfeksiyona karşı nodülasyon reaksiyonu göstermişlerdir. Ergin
hamamböceklerine fungal izolatlarla enfekte edilmeden hemen önce eikosanoid
biyosentez inhibitörleri enjekte edilmesi, fungal izolatlara karşı nodülasyon tepkisini
azaltmıştır. Eikosanoid biyosentezindeki enzimlerin inhibitörlerinden deksametazon (bir
fosfolipaz A2 inhibitörü), indometasin, naproksen, ibuprofen (siklooksijenaz
inhibitörleri), esculetin (bir lipoksijenaz inhibitörü) ve fenidon (hem
siklooksijenaz / hemde lipoksigenaz inhibitörü) de dahil olmak üzere spesifik
inhibitörlerle yapılan ayrı uygulamalar, B. germenica'nın fungal enfeksiyona
karşı nodül oluşturmasını azaltmıştır. Bu bulgular, eikosanoidlerin B.
germenica'nın erginlerindeki fungal enfeksiyonlara karşı nodülasyon
reaksiyonlarında rol aldığı hipotezini desteklemektedir.
Keywords
Böcek hücresel bağışıklığı Nodülasyon Beauveria bassiana Metarhizium anisopliae Blatella germanica
References
- Dunn, P.E. 1986. Biochemical aspects of insect immunology. Annu Rev Entomol, 31: 321-339.
- Gillespie, J.P., Kanost, M.R., Trenczek, T. 1997. Biological mediators of insect immunity. Annual Review of Entomology, 42: 611-643.
- Miller, J.S., Nguyen, T., Stanley-Samuelson, D,W. 1994. Eicosanoids mediate insect nodulation responses to bacterial infections. Proceedings of the National Acaddemy of Sciences USA, 91: 12418-12422.
- Rosales, C. 2017. Cellular and Molecular Mechanisms of Insect Immunity. In: Shields,V.D.C. Ed. Insect Physiology and Ecology. InTeach Publicaiton. CCBY. Pp. 179-212.
- Russell, V., Dunn, P.E. 1996. Antibacterial proteins in the midgut of Manduca sexta during metamorphosis. Journal of Insect Physiolology, 42: 65-71.
- SAS Institute Inc. 1989. SAS/STATR User's Guide, Version 6, 4th Ed., vol 2. SAS Institute Inc., Cary, NC.
- Satyavathi, V.V., Minz, A., Nagaraju, J. 2014. Nodulation: An unexplored cellular defense mechanism in insects. Cellular Signalling, 26: 1753-1763.
- Stanley, D.W. 2000. Eicosanoids in Invertebrate Signal Transduction Systems. Princeton University Press, Princeton, NJ.
- Stanley, D.W., Kim, Y. 2014. Eicosanoid Signaling in Insects: from Discovery to Plant Protection. Plant Sciences, 33:20-63.
- Stanley, D.W., Miller, J.S. 2006. Eicosanoid actions in insect cellular immune functions. Entomologia Experimentalis et Applicata, 119: 1-13.
- Tunaz, H. 2009. Eicosanoids act in nodulation reactions to bacteria Serratia marcescens in hemipteran pest, Eurygaster Integriceps Turkish Journal of Biology 33: 301-309.
- Tunaz, H., Bengin, C., Er, M.K. 2008. Nodulation reaction to fungal infections in larvae of Leptinotarsa decemlineata(Say) (Coleoptera: Chrysomelidae) mediated by eicosanoids. Turkish Journal of Agricultural Forestry 32: 11-18.
- Tunaz, H. 2006. Influence of Eicosanoids in nodulation reactions against bacteria, Serratia marcescens in larvae of Leptinotarsa decemlineata. KSU Fen Mühendislik Dergisi, 9:159-163.
Details
| Primary Language | Turkish |
|---|---|
| Journal Section | RESEARCH ARTICLE |
| Authors | |
| Publication Date | June 15, 2018 |
| Submission Date | October 25, 2017 |
| Acceptance Date | December 12, 2017 |
| Published in Issue | Year 2018Volume: 21 Issue: 3 |
Cite
Cited By
Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) Larvalarında Entomopatojenik Funguslara Karşı Nodülasyon Reaksiyonunda Eikosanoidlerin Rolü
Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi
Hasan TUNAZ
https://doi.org/10.18016/ksutarimdoga.vi.681089
International Peer Reviewed Journal
Free submission and publication
Published 6 times a year
KSU Journal of Agriculture and Nature
e-ISSN: 2619-9149