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Oil Hydrocarbon Degradation Capability of Bacterial Strains Isolated from the Sapanca Lake, Turkey

Year 2018, Volume: 21 Issue: 4, 535 - 544, 31.08.2018

Pelin S Çiftçi Türetken , Gülşen Altuğ Mine Çardak Kemal Güneş

https://doi.org/10.18016/ksudobil.344593
Cited By: 1

Abstract

In
this study, the analyses were carried out to determine oil-degradation capacity
and oil-resistance levels of bacteria isolated from water surface (0-30 cm) of
nine stations of the Sapanca Lake from September of 2008 to May of 2010.
Twenty-seven bacteria species belonging three classes and six families total of
eighty-five wild bacteria were identified and screened against crude oil with
respect to Minimum Inhibitory Concentration (MIC). Bacterial isolates showing
resistance against crude oil were chosen for Emulsification Index (E24)
test. Isolates displaying higher E24 values were selected for
further degradation tests regarding pH and oil thickness values in experimental setups. Fifty bacterial strains of
eighty-five isolates were recorded to be resistant against oil hydrocarbon.
Positive reactions percentages of the isolates against crude oil were detected
in variable ranges between 25% and 100%. The results of
the emulsification index test for crude oil indicated that the isolates of S49-Stenotrophomonas maltophila, S50-Aeromonas hydrophila, S59-E. coli1, S38-Aeromonas hydrophila and S43-Enterobacter
cloaceae have higher emulsification percentages. The results of the
degradation test showed that isolate of S59-E.
coli1 has higher degradation ability than all isolates tested. The
bacteria screened against crude oil were detected to be sensitive against crude
oil during the first year of the study. Presence of petroleum-resistant
bacteria in subsequent tests was associated with the unexpected oil spill
occurred in the Sapanca Lake at the time. Detected crude oil resistant bacteria
isolates were stocked for a possible use in upcoming bioremediation
related studies.  

Keywords

Crude oil wild bacterial isolates minimum inhibitory concentration Sapanca Lake

References

  • Akçaalan R, Köker L, Gürevin C, Albay M 2014. Planktothrix rubescens: A perennial presence and toxicity in Lake Sapanca. Turkish Journal of Botany, 38: 782-789.
  • APHA 2000. Standard Methods for the Examination of Water and Wastewater 20th Edition. Clesceri, L.S., A.E Greenberg and A.D Eaton (eds). American Public Health Association, American Water Works Association and Water Environment Federation. Washington, D.C.
  • ANON 1997. National Committee for Clinical Laboratory Standards. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. 4th ed. Publication M7-A2, Villanova, PA: National Committee for Clinical Laboratory Standards.
  • ANON 1991. U. S. Congress, Office of Technology Assesment, Bioemediation for Marine Oil Spills, OTA-BP-O-70 Washington, DC: U. S. Government Printing Office.
  • ANON 1998. Su Kalitesi Kontrolü, İzlenmesi ve Özel Çalışmalar: Sapanca Gölü ve Drenaj Alanı, DSI (General Directorate of State Hydraulic Works). 228-300, Ankara (In Turkish).
  • ANON 1984. Sapanca Gölü Kirlilik Çalışması (The Pollution Study of Sapanca Lake). DSİ (General Directorate of State Hydraulic Works). Technical Report 135, Ankara (In Turkish).
  • Akkoyunlu A, Akiner M 2012. Pollution evaluation in streams using water quality indices: A case study from Turkey’s Sapanca Lake Basin. Ecological Indicator, 18: 501–511.
  • Altug G, Gürün S, Yüksel B, Memon A 2011. The Investigation of Oil Degrading Capacity of Bacterial Strains Isolated from Different Environments in Turkey, Fresenius Environmental Bulletin, 20, 886-893.
  • Altug G, Gürün S, Yüksel B, Memon A 2012. Oil Hydrocarbon Degradation Effects of Some Bacteria Isolated from Various Environments in Turkey. First National Workshop on Marine Biotechnology and Genomics. 24-25 May 2012, Muğla-Turkey, pp. 10-25.
  • Atlas RM 1995. Petroleum Biodegradation and Oil Spill Bioremediation. Marine Pollution Bulletin, 31(4-12): 178-182. Bouwer EJ, Zehnder AJB 1993. Bioremediation of organic compounds – putting microbial metabolism to work, Trends in Biotechnology, 11, 360-367.
  • Ciftci PS, Altug G 2010. Study on the Degradation Ability of the Bacteria Isolated from the sea of Marmara in Oil Hydrocarbons, Turkey. 39th CIESM (Commission Internationale pour l’Exploration Scientifique de la mer Mediterranee) CongressRapp. Comm In. Mer Medit, 39, Venice-Italy. pp:346.
  • Cole JJ, Findlay S, Pace M L 1988. Bacterial production in fresh and saltwater ecosystems—a cross-system overview. Marine Ecology Progress Series, 43: 1–10.
  • Cotner JB, Biddanda BA 2002. Small players, large role: microbial influence on biogeochemical processes in pelagic aquatic ecosystems. Ecosystems, 5:105–121.
  • Das N, Chandran P 2011. Microbial Degradation of Petroleum Hydrocarbon Contaminants: An Overview. SAGE-Hindawi Access to Research Biotechnology Research International. Volume 2011, Article ID 941810, 13 pages. doi:10.4061/2011/941810.
  • Dean SM, Jin Y, Cha DK, Wilson SV, Radosevich M 2001. Phenanthrene Degradation in Soils Co-Inoculated with Phenanthrene-
  • Degrading and Biosurfactant-Producing Bacteria. Journal of Environmental Quality, 30: 1126-1133.
  • DeBruyn JM, Chewning CS, Sayler GS 2007. Comparative quantitative prevalence of Mycobacteria and functionally abundant nidA, nahAc, and nagAc dioxygenase genes in coal tar contaminated sediments. Environmental Science & Technology, 41: 5426–5432. doi: 10.1021/es070406c
  • Duman F, Aksoy A, Demirezen D 2007a. Seasonal Variability of Heavy Metals in Surface Sediment of Lake Sapanca, Turkey. Environmental Monitoring and Assessment, 133:277–283.
  • Duman F, Sezen G, Tug GN 2007b. Seasonal Changes of Some Heavy Metal Concentrations in Sapanca Lake Water, Turkey. International Journal of Natural & Engineering Sciences. 1(3): 25-28.
  • Ezikpe MNI, Gbenle OG, Ilori MO, Okpuzor J, Osuntoki AA 2009. Evaluation of Alcaligenes faecalis degradation of chrysene and diesel oil with concomitant production of biosurfactant. Research Journal of Environmental Toxicology,3: 159-169.
  • Farrington JW 1980. An overview of the biogeochemistry of fossil fuel hydrocarbons in the marine environment. Advances in Chemistry, 185: 1–22.
  • Head I, Jones D, Rolling W 2006. Marine Organisms make a meal of oil. Nature Reviews Microbiology, 4: 173-182.
  • Holmes B., Willcox WR, Lapage SP 1978. Identification of Enterobacteriaceae by the API 20E system. Journal of Clinical Pathology, 31(1): 22–30.
  • Ishige T, Tani A, Sakai Y, Kato N 2003. Wax ester production by bacteria. Current Opinion in Microbiology, 6(3): 244–250.
  • Jacques RJS, Okeke BC, Bento FM, Teixeira AS, Peralba MCR, Camargo FAO 2008. Microbial consortium bioaugmentation of a polycyclic aromatic hydrocarbons contaminated soil. Bioresource Technology, 99(7): 2637–2643.
  • Le-Petit J, Barthelemy MH 1968. The hydrocarbons in water: the problem of microorganisms using the littoral zones. Annales De l'Institut Pasteur, 144: 149–158.
  • Marchand C, St-Arnaud M, Hogland W, Bell TH, Hijri M 2017. Petroleum biodegradation capacity of bacteria and fungi isolated from petroleum-contaminated soil. International Biodeterioration & Biodegradation, 116: 48-57.
  • Morkoç E 2008. Water Quality Properties of Sapanca Lake. Ed. H. Okgerman, G. ALTUĞ. Scientific Overview of Sapanca Lake. Turkish Marine Research Foundation. No: 28. P: 140-148 (In Turkish).
  • Newton RJ, Jones SE, Eiler A, McMahon KD, Bertilsson S 2011. A guide to the natural history of freshwater lake bacteria. Microbiology and Molecular Biology Reviews, 75(1): 14-49 DOI: 10.1128/MMBR.00028-10.
  • Okoh AI, Trejo-Hernandez MR 2006. Remediation of petroleum hydrocarbon polluted systems: Exploiting the bioremediation strategies. African Journal of Biotechnology, 5(25): 2520–2525.
  • Onbasili D, Aslim B, Yuvali Celik G 2011. Investigation of metabolite productions and degradation of hazardous organic pollutants by Pseudomonas spp. Journal of Applied Biological Sciences, 5(2): 9–14.
  • Prieto LM, Michelon M, Burkert JFM, Kalil SJ, Burker CAV 2008. The production of rhamnolipid by a Pseudomonas aeruginosa strain isolated from a southern coastal zone in Brazil. Chemosphere, 71(9): 1781-1785.
  • Pritchard HP, Costa CF 1991. EPA's Alaska oil spill bioremediation project. Environmental Science & Technology, 25(3): 372-379. Rahman KSM, Rahman JT, Lakshmanaperumalsamy P, Banat IM 2002. Towards efficient crude oil degredation by a mixed bacterial consortium, Bioresource Technology, 85: 257-261.
  • Ron EZ, Rosenberg E 2002. Biosurfactants and oil bioremediation. Environmental Biotechnology, 13(3): 249-252. Sarubbo LA 2006. Production and stability studies of the bioemulsifier obtained from a strain of Candida glabrata UCO1002. Journal of Biotechnology, 9: 400-406.
  • Tabatabaee A, Assadi MM, Noohi AA, Sajadian VA 2005. Isolation of biosurfactant producing bacteria from oil reservoirs, Iranian Journal of Environmental Health Science & Engineering, 2: 6-12.
  • Tanik A, Beler Baykal B, Gönenc E, Meric S, Öktem Y 1998. Effect and Control of Pollution in Catchment Area of Lake Sapanca, Turkey. Environmental Management, 22(3): 407–414.
  • Udo EJ, Fayemi AAA 1995. The effect of oil pollution on soil germination, growth and nutrient uptake of corn. Journal of Environmental Quality, 4: 537-540.
  • Yakimov MM, Timmis KN, Golyshin PN 2007. Obligate oil‐degrading marine bacteria. Current Opinion in Biotechnology, 18:257–266.
  • Zarate MGM, González JFC, Pérez ASR, Domínguez EE, Oviedo JT, Juárez VMM, Rodríguez IA 2014. Isolation and Identification of Bacteria and Fungi Resistant to Crude Oil. Journal of Multidisciplinary Engineering Science and Technology, 3(7): 5273-5278.

Sapanca Gölü’nden İzole Edilen Bakteri Suşlarının Petrol Hidrokarbonlarını Parçalama Yetenekleri

Year 2018, Volume: 21 Issue: 4, 535 - 544, 31.08.2018

Pelin S Çiftçi Türetken , Gülşen Altuğ Mine Çardak Kemal Güneş

https://doi.org/10.18016/ksudobil.344593
Cited By: 1

Abstract

Bu
çalışmada, Eylül 2008 ile Mayıs 2010 tarihleri arasında Sapanca Gölü’nde
belirlenen dokuz istasyonda yüzey sularından (0-30 cm) izole edilen
bakterilerin petrol parçalama kapasiteleri ve direnç düzeylerini belirlemek
amacıyla analizler yapılmıştır. Üç sınıf yedi familyaya ait yirmi yedi bakteri
türünden oluşan toplam seksen beş doğal bakteri izolatı tanımlanmış ve bu
izolatların hepsi Minimum İnhibitör Konsantrasyon (MİK) açısından ham petrole
karşı taranmıştır. Emülsifikasyon indeksi (E24) testi için ham
petrole karşı direnç gösteren bakteri izolatları seçilmiştir. E24 testlerinde
yüksek değerler gösteren izolatlar ileri parçalama testlerinde pH ve petrol
katman kalınlığı değerleri kaydedilmek üzere seçilmiştir. Seksen beş izolattan
elli tanesi petrol hidrokarbonuna karşı dirençli olarak kaydedilmiştir. Ham
petrole karşı pozitif reaksiyon gösteren izolatların yüzdeleri %25 ile %100
arasında değişiklik göstermiştir. S49-Stenotrophomonas maltophila, S50-Aeromonas hydrophila, S59-E. coli1, S38-Aeromonas hydrophila and S43-Enterobacter
cloaceae suşları en yüksek Emülsifikasyon İndeksi yüzdeleri gösteren
izolatlar olarak kaydedilmiştir. Parçalanma testinin sonuçları, S59-E coli1 izolatının test edilen tüm
izolatlardan daha yüksek parçalama kabiliyetine sahip olduğunu göstermiştir.
Çalışmanın ilk yılında yapılan ham petrole karşı dirençlilik testlerinde taranan
bakterilerin tümünün duyarlı olduğu kaydedilmiştir. Daha sonraki testlerde
petrole karşı direnç gösteren bakterilerin tespit edilmesi aynı dönemde Sapanca
Gölü’nde meydana gelen beklenmedik petrol sızıntısı ile ilişkilendirilmiştir.
Ham petrole dirençli izolatlar, aday bakteriler olarak belirlenmiş ve ileride
olası biyoremediasyon çalışmalarında kullanılmak amacıyla stoklanmıştır.

Keywords

Ham petrol doğal bakteri izolatları minimum inhibisyon konsantrasyonu Sapanca Gölü

References

  • Akçaalan R, Köker L, Gürevin C, Albay M 2014. Planktothrix rubescens: A perennial presence and toxicity in Lake Sapanca. Turkish Journal of Botany, 38: 782-789.
  • APHA 2000. Standard Methods for the Examination of Water and Wastewater 20th Edition. Clesceri, L.S., A.E Greenberg and A.D Eaton (eds). American Public Health Association, American Water Works Association and Water Environment Federation. Washington, D.C.
  • ANON 1997. National Committee for Clinical Laboratory Standards. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. 4th ed. Publication M7-A2, Villanova, PA: National Committee for Clinical Laboratory Standards.
  • ANON 1991. U. S. Congress, Office of Technology Assesment, Bioemediation for Marine Oil Spills, OTA-BP-O-70 Washington, DC: U. S. Government Printing Office.
  • ANON 1998. Su Kalitesi Kontrolü, İzlenmesi ve Özel Çalışmalar: Sapanca Gölü ve Drenaj Alanı, DSI (General Directorate of State Hydraulic Works). 228-300, Ankara (In Turkish).
  • ANON 1984. Sapanca Gölü Kirlilik Çalışması (The Pollution Study of Sapanca Lake). DSİ (General Directorate of State Hydraulic Works). Technical Report 135, Ankara (In Turkish).
  • Akkoyunlu A, Akiner M 2012. Pollution evaluation in streams using water quality indices: A case study from Turkey’s Sapanca Lake Basin. Ecological Indicator, 18: 501–511.
  • Altug G, Gürün S, Yüksel B, Memon A 2011. The Investigation of Oil Degrading Capacity of Bacterial Strains Isolated from Different Environments in Turkey, Fresenius Environmental Bulletin, 20, 886-893.
  • Altug G, Gürün S, Yüksel B, Memon A 2012. Oil Hydrocarbon Degradation Effects of Some Bacteria Isolated from Various Environments in Turkey. First National Workshop on Marine Biotechnology and Genomics. 24-25 May 2012, Muğla-Turkey, pp. 10-25.
  • Atlas RM 1995. Petroleum Biodegradation and Oil Spill Bioremediation. Marine Pollution Bulletin, 31(4-12): 178-182. Bouwer EJ, Zehnder AJB 1993. Bioremediation of organic compounds – putting microbial metabolism to work, Trends in Biotechnology, 11, 360-367.
  • Ciftci PS, Altug G 2010. Study on the Degradation Ability of the Bacteria Isolated from the sea of Marmara in Oil Hydrocarbons, Turkey. 39th CIESM (Commission Internationale pour l’Exploration Scientifique de la mer Mediterranee) CongressRapp. Comm In. Mer Medit, 39, Venice-Italy. pp:346.
  • Cole JJ, Findlay S, Pace M L 1988. Bacterial production in fresh and saltwater ecosystems—a cross-system overview. Marine Ecology Progress Series, 43: 1–10.
  • Cotner JB, Biddanda BA 2002. Small players, large role: microbial influence on biogeochemical processes in pelagic aquatic ecosystems. Ecosystems, 5:105–121.
  • Das N, Chandran P 2011. Microbial Degradation of Petroleum Hydrocarbon Contaminants: An Overview. SAGE-Hindawi Access to Research Biotechnology Research International. Volume 2011, Article ID 941810, 13 pages. doi:10.4061/2011/941810.
  • Dean SM, Jin Y, Cha DK, Wilson SV, Radosevich M 2001. Phenanthrene Degradation in Soils Co-Inoculated with Phenanthrene-
  • Degrading and Biosurfactant-Producing Bacteria. Journal of Environmental Quality, 30: 1126-1133.
  • DeBruyn JM, Chewning CS, Sayler GS 2007. Comparative quantitative prevalence of Mycobacteria and functionally abundant nidA, nahAc, and nagAc dioxygenase genes in coal tar contaminated sediments. Environmental Science & Technology, 41: 5426–5432. doi: 10.1021/es070406c
  • Duman F, Aksoy A, Demirezen D 2007a. Seasonal Variability of Heavy Metals in Surface Sediment of Lake Sapanca, Turkey. Environmental Monitoring and Assessment, 133:277–283.
  • Duman F, Sezen G, Tug GN 2007b. Seasonal Changes of Some Heavy Metal Concentrations in Sapanca Lake Water, Turkey. International Journal of Natural & Engineering Sciences. 1(3): 25-28.
  • Ezikpe MNI, Gbenle OG, Ilori MO, Okpuzor J, Osuntoki AA 2009. Evaluation of Alcaligenes faecalis degradation of chrysene and diesel oil with concomitant production of biosurfactant. Research Journal of Environmental Toxicology,3: 159-169.
  • Farrington JW 1980. An overview of the biogeochemistry of fossil fuel hydrocarbons in the marine environment. Advances in Chemistry, 185: 1–22.
  • Head I, Jones D, Rolling W 2006. Marine Organisms make a meal of oil. Nature Reviews Microbiology, 4: 173-182.
  • Holmes B., Willcox WR, Lapage SP 1978. Identification of Enterobacteriaceae by the API 20E system. Journal of Clinical Pathology, 31(1): 22–30.
  • Ishige T, Tani A, Sakai Y, Kato N 2003. Wax ester production by bacteria. Current Opinion in Microbiology, 6(3): 244–250.
  • Jacques RJS, Okeke BC, Bento FM, Teixeira AS, Peralba MCR, Camargo FAO 2008. Microbial consortium bioaugmentation of a polycyclic aromatic hydrocarbons contaminated soil. Bioresource Technology, 99(7): 2637–2643.
  • Le-Petit J, Barthelemy MH 1968. The hydrocarbons in water: the problem of microorganisms using the littoral zones. Annales De l'Institut Pasteur, 144: 149–158.
  • Marchand C, St-Arnaud M, Hogland W, Bell TH, Hijri M 2017. Petroleum biodegradation capacity of bacteria and fungi isolated from petroleum-contaminated soil. International Biodeterioration & Biodegradation, 116: 48-57.
  • Morkoç E 2008. Water Quality Properties of Sapanca Lake. Ed. H. Okgerman, G. ALTUĞ. Scientific Overview of Sapanca Lake. Turkish Marine Research Foundation. No: 28. P: 140-148 (In Turkish).
  • Newton RJ, Jones SE, Eiler A, McMahon KD, Bertilsson S 2011. A guide to the natural history of freshwater lake bacteria. Microbiology and Molecular Biology Reviews, 75(1): 14-49 DOI: 10.1128/MMBR.00028-10.
  • Okoh AI, Trejo-Hernandez MR 2006. Remediation of petroleum hydrocarbon polluted systems: Exploiting the bioremediation strategies. African Journal of Biotechnology, 5(25): 2520–2525.
  • Onbasili D, Aslim B, Yuvali Celik G 2011. Investigation of metabolite productions and degradation of hazardous organic pollutants by Pseudomonas spp. Journal of Applied Biological Sciences, 5(2): 9–14.
  • Prieto LM, Michelon M, Burkert JFM, Kalil SJ, Burker CAV 2008. The production of rhamnolipid by a Pseudomonas aeruginosa strain isolated from a southern coastal zone in Brazil. Chemosphere, 71(9): 1781-1785.
  • Pritchard HP, Costa CF 1991. EPA's Alaska oil spill bioremediation project. Environmental Science & Technology, 25(3): 372-379. Rahman KSM, Rahman JT, Lakshmanaperumalsamy P, Banat IM 2002. Towards efficient crude oil degredation by a mixed bacterial consortium, Bioresource Technology, 85: 257-261.
  • Ron EZ, Rosenberg E 2002. Biosurfactants and oil bioremediation. Environmental Biotechnology, 13(3): 249-252. Sarubbo LA 2006. Production and stability studies of the bioemulsifier obtained from a strain of Candida glabrata UCO1002. Journal of Biotechnology, 9: 400-406.
  • Tabatabaee A, Assadi MM, Noohi AA, Sajadian VA 2005. Isolation of biosurfactant producing bacteria from oil reservoirs, Iranian Journal of Environmental Health Science & Engineering, 2: 6-12.
  • Tanik A, Beler Baykal B, Gönenc E, Meric S, Öktem Y 1998. Effect and Control of Pollution in Catchment Area of Lake Sapanca, Turkey. Environmental Management, 22(3): 407–414.
  • Udo EJ, Fayemi AAA 1995. The effect of oil pollution on soil germination, growth and nutrient uptake of corn. Journal of Environmental Quality, 4: 537-540.
  • Yakimov MM, Timmis KN, Golyshin PN 2007. Obligate oil‐degrading marine bacteria. Current Opinion in Biotechnology, 18:257–266.
  • Zarate MGM, González JFC, Pérez ASR, Domínguez EE, Oviedo JT, Juárez VMM, Rodríguez IA 2014. Isolation and Identification of Bacteria and Fungi Resistant to Crude Oil. Journal of Multidisciplinary Engineering Science and Technology, 3(7): 5273-5278.
There are 39 citations in total.

Details

Primary Language English
Journal Section RESEARCH ARTICLE
Authors

Pelin S Çiftçi Türetken

Gülşen Altuğ

Mine Çardak

Kemal Güneş

Publication Date August 31, 2018
Submission Date October 20, 2017
Acceptance Date February 26, 2018
Published in Issue Year 2018Volume: 21 Issue: 4

Cite

APA Çiftçi Türetken, P. S., Altuğ, G., Çardak, M., Güneş, K. (2018). Oil Hydrocarbon Degradation Capability of Bacterial Strains Isolated from the Sapanca Lake, Turkey. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 21(4), 535-544. https://doi.org/10.18016/ksudobil.344593

Cited By

Total Petroleum Hydrocarbons (TPH) Levels in the Surface Water of Lake Sapanca, Turkey
Journal of Anatolian Environmental and Animal Sciences
Pelin Saliha ÇİFTÇİ TÜRETKEN
https://doi.org/10.35229/jaes.572803
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