Cytotoxic Effect of Rhododendron luteum Leaf Extract on Human Cancer Cell Lines

Selim Demir; İbrahim Turan; Yuksel Alıyazıcıoglu

doi:10.18016/ksutarimdoga.vi.421283

Research Article

Rhododendron luteum Yaprak Ekstraktının İnsan Kanser Hücre Serilerindeki Sitotoksik Etkisi

Year 2018, Volume: 21 Issue: 6, 950 - 956, 30.11.2018

Selim Demir , İbrahim Turan , Yuksel Alıyazıcıoglu

https://doi.org/10.18016/ksutarimdoga.vi.421283

Cited By: 13

Abstract

Rhododendron
luteum, Rhododendron cinsine dahil bir bitki
olup, bu cinse ait bitkiler geleneksel tedavide sıklıkla kullanılmaktadır. Rhododendron türlerinin sitotoksik
etkilerini araştıran çok sayıda çalışma olmasına rağmen, R. luteum türünün sitotoksik etkilerini inceleyen çalışmalar
sınırlıdır. Bu çalışmanın amacı R. luteum
yaprak ekstraktının antioksidan özelliklerini ve insan kanser hücreleri üzerindeki
sitotoksik etkisini araştırmaktır. Ekstraktın toplam fenolik içeriği (TPC),
toplam flavonoid içeriği (TFC) ve indirgeyici gücü spektrofotometrik
prosedürler kullanılarak değerlendirildi. Ekstraktın beş kanser (insan meme,
kolon, akciğer, prostat ve karaciğer karsinomu) ve insan fibroblast hücreleri
üzerindeki sitotoksik etkisi MTT analizi kullanılarak belirlendi. Ekstraktın
TPC ve indirgeyici güç değerleri g örnek başına sırasıyla 173.2±3.97 mg gallik
asit eşdeğeri ve 629.5±3.49 mg troloks eşdeğeri olarak bulundu. R. luteum yaprak ekstraktı normal
fibroblast hücrelerine kıyasla özellikle kolon (1.9 kat) ve karaciğer (2.2 kat)
kanser hücrelerine karşı seçici sitotoksisite sergiledi. Bu çalışma R. luteum yaprak ekstraktının
antiproliferatif etkisini ortaya koyan ilk çalışmadır. Ekstraktta bulunan
sitotoksik moleküllerin tanımlanması ve etki mekanizmalarının belirlenmesi için
daha kapsamlı çalışmalara ihtiyaç vardır.

Keywords

Antioksidan aktivite, Fenolikler, Kanser, Rhododendron luteum, Sitotoksisite

References

Ali S, Nisar M, Qaisar M, Khan A, Khan AA, 2017. Evaluation of the cytotoxic potential of a new pentacyclic triterpene from Rhododendron arboreum stem bark. Pharm Biol, 55(1): 1927-1930.
Aliyazicioglu R, Demir S, Badem M, Sener SO, Korkmaz N, Ayazoglu Demir E, Ozgen U, Alpay Karaoglu S, Aliyazicioglu Y, 2017. Antioxidant, antigenotoxic, antimicrobial activities and phytochemical analysis of Dianthus carmelitarum. Rec Nat Prod, 11(3): 270-284.
Bilir EK, Tutun H, Sevı̇n S, Kısmalı G, Yarsan E, 2018. Cytotoxic effects of Rhododendron ponticum L. extract on prostate carcinoma and adenocarcinoma cell line (DU145, PC3). Kafkas Univ Vet Fak Derg, 24(3): 451-457.
Byun KS, Lee YW, Jin HJ, Lee MK, Lee HY, Lee KJ, Heo MY, Yu CY, Lee JH, 2005. Genotoxicity and cytotoxicity in human cancer and normal cell lines of the extracts of Rhododendron brachycarpum D. Don leaves. Korean J Medicinal Crop Sci, 13(4): 199-205.
Demir S, Turan I, Aliyazicioglu Y, 2016a. Selective cytotoxic effect of Rhododendron luteum extract on human colon and liver cancer cells. J BUON, 21(4): 883-888.
Demir S, Aliyazicioglu Y, Turan I, Misir S, Mentese A, Yaman SO, Akbulut K, Kilinc K, Deger O, 2016b. Antiproliferative and proapoptotic activity of Turkish propolis on human lung cancer cell line. Nutr Cancer, 68(1): 165-172.
Demir S, Turan I, Demir F, Ayazoglu Demir E, Aliyazicioglu Y, 2017. Cytotoxic effect of Laurocerasus officinalis extract on human cancer cell lines. Marmara Pharm J, 21: 121-126.
Jang GU, Choi SU, Lee KR, 2005. Cytotoxic constituents of Rhododendron brachycarpum. Yakhak Hoechi, 49: 244-248.
Jing L, Ma H, Fan P, Gao R, Jia Z, 2015. Antioxidant potential, total phenolic and total flavonoid contents of Rhododendron anthopogonoides and its protective effect on hypoxia-induced injury in PC12 cells. BMC Complement Altern Med, 15: 287, DOI: 10.1186/s12906-015-0820-3.
Lee SH, Sancheti SA, Bafna MR, Sancheti SS, Seo SY, 2011. Acetylcholineterase inhibitory and antioxidant properties of Rhododendron yedoense var. Poukhanense bark. J Med Plants Res, 5(2): 248-254.
Li FR, Yu FX, Yao ST, Si YH, Zhang W, Gao LL, 2012. Hyperin extracted from Manchurian Rhododendron leaf induces apoptosis in human endometrial cancer cells through a mitochondrial pathway. Asian Pac J Cancer Prev, 13: 3653-3656.
Lin CY, Lin LC, Ho ST, Tung YT, Tseng YH, Wu JH, 2014. Antioxidant activities and phytochemicals of leaf extracts from 10 native Rhododendron species in Taiwan. Evid Based Complementary Altern Med, Article ID: 283938, DOI: 10.1155/2014/283938.
ManiKumar P, Pardhu G, Ravinder S, Lakshmi V, Tejaswini K, Vishwanath Pradeep B, 2011. Phytochemical screening, antimicrobial and antiproliferative properties of Rhododendron ponticum on prostate epithelial cancer cells. J Pharm Sci & Res, 3(11): 1552-1555.
Moreno MI, Isla MI, Sampietro AR, Vattuone MA, 2000. Comparison of the free radical- scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol, 71(1-2): 109-114.
Mosmann T, 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods, 65: 55-63.
Nema R, Khare S, Jain P, Pradhan A, Gupta A, Singh D, 2013. Natural products potential and scope for modern cancer research. AJPS, 4: 1270-1277.
Niedzwiecki A, Roomi MW, Kalinovsky T, Rath M, 2016. Anticancer efficacy of polyphenols and their combinations. Nutrients, 8: 552, DOI:10.3390/nu8090552.
Oyaizu M, 1986. Studies on product of browning reaction prepared from glucoseamine. Jpn J Nutr, 44: 307-315.
Ozkan MT, Aliyazicioglu R, Demir S, Misir S, Turan I, Yildirmis S, Aliyazicioglu Y, 2017. Phenolic characterisation and antioxidant activity of Primula vulgaris and its antigenotoxic effect on fibroblast cells. Jundishapur J Nat Pharm Prod, 12(1): e40073, DOI: 10.5812/jjnpp.40073.
Park YH, Kim SM, 2008. Composition and cytotoxicity of essential oil from Korean Rhododendron (Rhododendon mucronulatum Turcz. var. ciliatum Nakai). J Korean Soc Appl Biol Chem, 51: 233-237.
Popescu R, Kopp B, 2013. The genus Rhododendron: An ethnopharmacological and toxicological review. J Ethnopharmacol, 147: 42-62.
Prakash O, Kumar A, Kumar P, Ajeet, 2013. Anticancer potential of plants and natural products: A review. Am J Pharmacol Sci, 1(6): 104-115.
Qiang Y, Zhou B, Gao K, 2011. Chemical constituents of plants from the genus Rhododendron. Chem Biodivers, 8: 792-815.
Rayan A, Raiyn J, Falah M, 2017. Nature is the best source of anticancer drugs: Indexing natural products for their anticancer bioactivity. PlosOne, 12(11): e0187925, DOI: 10.1371/journal.pone.0187925.
Russo GL, Tedesco I, Spagnuolo C, Russo M, 2017. Antioxidant polyphenols in cancer treatment: Friend, foe or foil? Semin Cancer Biol, 46: 1-13.
Seephonkai P, Popescu R, Zehl M, Krupitza G, Urban E, Kopp B, 2011. Ferruginenes A-C from Rhododendron ferrugineum and their cytotoxic evaluation. J Nat Prod, 74: 712-717.
Slinkard K, Singleton VL, 1977. Total phenol analyses: automation and comparison with manual methods. Am J Enol Viticult, 28: 49-55.
Taraphdar AK, Roy M, Bhattacharya RK, 2001. Natural products as inducers of apoptosis: Implication for cancer therapy and prevention. Current Science, 80(11): 1387-1396.
Way TD, Tsai SJ, Wang CM, Ho CT, Chou CH, 2014. Chemical constituents of Rhododendron formosanum show pronounced growth inhibitory effect on non-small-cell lung carcinoma cells. J Agric Food Chem, 62: 875-884.
Yaylaci F, Kolayli S, Kucuk M, Karaoglu Alpay S, Ulusoy E, 2007. Biological activities of trunk bark extracts of five tree species from Anatolia, Turkey. Asian J Chem, 19(3): 2241-2256.
Yuan R, Hou Y, Sun W, Yu J, Liu X, Niu Y, Lu JJ, Chen X, 2017. Natural products to prevent drug resistance in cancer chemotherapy: a review. Ann N Y Acad Sci, 1401: 19-27.
Zhou Y, Zheng J, Li Y, Xu DP, Li S, Chen YM, Li HB, 2016. Natural polyphenols for prevention and treatment of cancer. Nutrients, 8: 515, DOI:10.3390/nu8080515.

Cytotoxic Effect of Rhododendron luteum Leaf Extract on Human Cancer Cell Lines

Year 2018, Volume: 21 Issue: 6, 950 - 956, 30.11.2018

Selim Demir , İbrahim Turan , Yuksel Alıyazıcıoglu

https://doi.org/10.18016/ksutarimdoga.vi.421283

Cited By: 13

Abstract

Rhododendron luteum belongs to the genus Rhododendron, members
of which are frequently used in folk medicine. Various studies have
investigated the cytotoxic effect of different Rhododendron species, but
there have been limited studies of the cytotoxic effect of R. luteum.
The aim of this study was to investigate the antioxidant properties and
cytotoxic effect of R. luteum leaf extract on the human cancer cells.
The total phenolic content (TPC), total flavonoid content (TFC), and reducing
power of the extract were evaluated using spectrophotometric procedures. The
cytotoxic effect of the extract on five cancers (human breast, colon, lung,
prostate, and liver carcinoma) and human fibroblast cells was determined using
the MTT assay. TPC and reducing power values of extract were found 173.2±3.97
mg gallic acid equivalents, and 629.5±3.49 mg trolox equivalents per to g
sample, respectively. R. luteum leaf
extract exhibited selective cytotoxicity especially against colon (1.9 fold)
and liver (2.2 fold) cancer cells compared to normal fibroblast cells. This is
the first study to reveal the antiproliferative effect of R. luteum leaf extract. Further studies are now needed to identify
the cytotoxic molecules in the extract and their mechanisms.

Keywords

Antioxidant activity, Cancer, Cytotoxicity, Phenolics, Rhododendron luteum

References

Ali S, Nisar M, Qaisar M, Khan A, Khan AA, 2017. Evaluation of the cytotoxic potential of a new pentacyclic triterpene from Rhododendron arboreum stem bark. Pharm Biol, 55(1): 1927-1930.
Aliyazicioglu R, Demir S, Badem M, Sener SO, Korkmaz N, Ayazoglu Demir E, Ozgen U, Alpay Karaoglu S, Aliyazicioglu Y, 2017. Antioxidant, antigenotoxic, antimicrobial activities and phytochemical analysis of Dianthus carmelitarum. Rec Nat Prod, 11(3): 270-284.
Bilir EK, Tutun H, Sevı̇n S, Kısmalı G, Yarsan E, 2018. Cytotoxic effects of Rhododendron ponticum L. extract on prostate carcinoma and adenocarcinoma cell line (DU145, PC3). Kafkas Univ Vet Fak Derg, 24(3): 451-457.
Byun KS, Lee YW, Jin HJ, Lee MK, Lee HY, Lee KJ, Heo MY, Yu CY, Lee JH, 2005. Genotoxicity and cytotoxicity in human cancer and normal cell lines of the extracts of Rhododendron brachycarpum D. Don leaves. Korean J Medicinal Crop Sci, 13(4): 199-205.
Demir S, Turan I, Aliyazicioglu Y, 2016a. Selective cytotoxic effect of Rhododendron luteum extract on human colon and liver cancer cells. J BUON, 21(4): 883-888.
Demir S, Aliyazicioglu Y, Turan I, Misir S, Mentese A, Yaman SO, Akbulut K, Kilinc K, Deger O, 2016b. Antiproliferative and proapoptotic activity of Turkish propolis on human lung cancer cell line. Nutr Cancer, 68(1): 165-172.
Demir S, Turan I, Demir F, Ayazoglu Demir E, Aliyazicioglu Y, 2017. Cytotoxic effect of Laurocerasus officinalis extract on human cancer cell lines. Marmara Pharm J, 21: 121-126.
Jang GU, Choi SU, Lee KR, 2005. Cytotoxic constituents of Rhododendron brachycarpum. Yakhak Hoechi, 49: 244-248.
Jing L, Ma H, Fan P, Gao R, Jia Z, 2015. Antioxidant potential, total phenolic and total flavonoid contents of Rhododendron anthopogonoides and its protective effect on hypoxia-induced injury in PC12 cells. BMC Complement Altern Med, 15: 287, DOI: 10.1186/s12906-015-0820-3.
Lee SH, Sancheti SA, Bafna MR, Sancheti SS, Seo SY, 2011. Acetylcholineterase inhibitory and antioxidant properties of Rhododendron yedoense var. Poukhanense bark. J Med Plants Res, 5(2): 248-254.
Li FR, Yu FX, Yao ST, Si YH, Zhang W, Gao LL, 2012. Hyperin extracted from Manchurian Rhododendron leaf induces apoptosis in human endometrial cancer cells through a mitochondrial pathway. Asian Pac J Cancer Prev, 13: 3653-3656.
Lin CY, Lin LC, Ho ST, Tung YT, Tseng YH, Wu JH, 2014. Antioxidant activities and phytochemicals of leaf extracts from 10 native Rhododendron species in Taiwan. Evid Based Complementary Altern Med, Article ID: 283938, DOI: 10.1155/2014/283938.
ManiKumar P, Pardhu G, Ravinder S, Lakshmi V, Tejaswini K, Vishwanath Pradeep B, 2011. Phytochemical screening, antimicrobial and antiproliferative properties of Rhododendron ponticum on prostate epithelial cancer cells. J Pharm Sci & Res, 3(11): 1552-1555.
Moreno MI, Isla MI, Sampietro AR, Vattuone MA, 2000. Comparison of the free radical- scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol, 71(1-2): 109-114.
Mosmann T, 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods, 65: 55-63.
Nema R, Khare S, Jain P, Pradhan A, Gupta A, Singh D, 2013. Natural products potential and scope for modern cancer research. AJPS, 4: 1270-1277.
Niedzwiecki A, Roomi MW, Kalinovsky T, Rath M, 2016. Anticancer efficacy of polyphenols and their combinations. Nutrients, 8: 552, DOI:10.3390/nu8090552.
Oyaizu M, 1986. Studies on product of browning reaction prepared from glucoseamine. Jpn J Nutr, 44: 307-315.
Ozkan MT, Aliyazicioglu R, Demir S, Misir S, Turan I, Yildirmis S, Aliyazicioglu Y, 2017. Phenolic characterisation and antioxidant activity of Primula vulgaris and its antigenotoxic effect on fibroblast cells. Jundishapur J Nat Pharm Prod, 12(1): e40073, DOI: 10.5812/jjnpp.40073.
Park YH, Kim SM, 2008. Composition and cytotoxicity of essential oil from Korean Rhododendron (Rhododendon mucronulatum Turcz. var. ciliatum Nakai). J Korean Soc Appl Biol Chem, 51: 233-237.
Popescu R, Kopp B, 2013. The genus Rhododendron: An ethnopharmacological and toxicological review. J Ethnopharmacol, 147: 42-62.
Prakash O, Kumar A, Kumar P, Ajeet, 2013. Anticancer potential of plants and natural products: A review. Am J Pharmacol Sci, 1(6): 104-115.
Qiang Y, Zhou B, Gao K, 2011. Chemical constituents of plants from the genus Rhododendron. Chem Biodivers, 8: 792-815.
Rayan A, Raiyn J, Falah M, 2017. Nature is the best source of anticancer drugs: Indexing natural products for their anticancer bioactivity. PlosOne, 12(11): e0187925, DOI: 10.1371/journal.pone.0187925.
Russo GL, Tedesco I, Spagnuolo C, Russo M, 2017. Antioxidant polyphenols in cancer treatment: Friend, foe or foil? Semin Cancer Biol, 46: 1-13.
Seephonkai P, Popescu R, Zehl M, Krupitza G, Urban E, Kopp B, 2011. Ferruginenes A-C from Rhododendron ferrugineum and their cytotoxic evaluation. J Nat Prod, 74: 712-717.
Slinkard K, Singleton VL, 1977. Total phenol analyses: automation and comparison with manual methods. Am J Enol Viticult, 28: 49-55.
Taraphdar AK, Roy M, Bhattacharya RK, 2001. Natural products as inducers of apoptosis: Implication for cancer therapy and prevention. Current Science, 80(11): 1387-1396.
Way TD, Tsai SJ, Wang CM, Ho CT, Chou CH, 2014. Chemical constituents of Rhododendron formosanum show pronounced growth inhibitory effect on non-small-cell lung carcinoma cells. J Agric Food Chem, 62: 875-884.
Yaylaci F, Kolayli S, Kucuk M, Karaoglu Alpay S, Ulusoy E, 2007. Biological activities of trunk bark extracts of five tree species from Anatolia, Turkey. Asian J Chem, 19(3): 2241-2256.
Yuan R, Hou Y, Sun W, Yu J, Liu X, Niu Y, Lu JJ, Chen X, 2017. Natural products to prevent drug resistance in cancer chemotherapy: a review. Ann N Y Acad Sci, 1401: 19-27.
Zhou Y, Zheng J, Li Y, Xu DP, Li S, Chen YM, Li HB, 2016. Natural polyphenols for prevention and treatment of cancer. Nutrients, 8: 515, DOI:10.3390/nu8080515.

There are 32 citations in total.

Details

Primary Language	English
Journal Section	RESEARCH ARTICLE
Authors	Selim Demir 0000-0002-1863-6280 İbrahim Turan 0000-0003-3400-5494 Yuksel Alıyazıcıoglu 0000-0001-9474-4307
Publication Date	November 30, 2018
Submission Date	May 5, 2018
Acceptance Date	June 19, 2018
Published in Issue	Year 2018Volume: 21 Issue: 6

Cite

APA	Demir, S., Turan, İ., & Alıyazıcıoglu, Y. (2018). Cytotoxic Effect of Rhododendron luteum Leaf Extract on Human Cancer Cell Lines. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 21(6), 950-956. https://doi.org/10.18016/ksutarimdoga.vi.421283