Effects of Beauveria bassiana on Quantity of F1 Larvae and Larval Mortality of Tribolium confusum (Coleoptera: Tenebrionidae)
Abstract
In this study, larval
mortality of Tribolium confusum (Coleoptera:
Tenebrionidae) due to Beauveria bassiana
infections and the number of larvae produced in the presence of B. bassiana spores were investigated.
Larvae were kept in wheat treated with spores and mortalities were assessed. In
a long-term experiment, number of larvae and mortality was recorded after 3
months. Within 7 days, larval mortality was 55% at 250ppm and over 75% at
higher concentrations. The mortality reached 100% on day 14 in all fungus
treatments. After the adults were kept for 14 and 28 days, 6.3 and 12.0 larvae
in treatments and 1 and 6.3 larvae in control units were counted, respectively.
In the long-term experiment, number of larvae was 10.3, 42.0 and 260.3 in the
control, 500ppm and 1000ppm treatments, respectively. Larval mortalities were
%97.4 in 500ppm and 100% in 1000ppm treatments.
The results show that although B.
bassiana treatment increases the number of T. confusum larvae as the concentration increases, B. bassiana treatment also cause high
levels of larval mortalities depending on concentration and time, suggesting
that, B. basssiana treatment can have
a role in controlling T. confusum
populations.
Keywords
Biological control Microbial control Stored-product pest Tribolium confusum Beauveria bassiana
Supporting Institution
Scientific Research Projects Unit of Kahramanmaras Sutcu Imam University
Project Number
Project No: 2016/5-42 YLS
Thanks
This study was a part of a project granted by Scientific Research Projects Unit of Kahramanmaras Sutcu Imam University (Project No: 2016/5-42 YLS).
References
- Akbar W, Lord JC, Nechols JR, Howard RW 2004. Diatomaceous Earth Increases the Efficacy of Beauveria bassiana Against Tribolium castaneum Larvae and Increases Conidia Attachment. Journal of Economic Entomology, 97: 273-280.
- Akbar W, Lord JC, Nechols JR, Loughin TM 2005. Efficacy of Beauveria bassiana for Red Flour Beetle When Applied with Plant Essential Oils or in Mineral Oil and Organosilicone Carriers. Journal of Economic Entomology, 98: 683-688.
- Barış C 2016. The Use of Some Cereals on The Production of Beauveria bassiana By Solid State Fermentation Technique. University of Kahramanmaraş Sütçü İmam, Department of Plant Protection, M.Sc. thesis, Turkey.
- Barra R, Rosso L, Nesci A, Etcheverry M 2013. Isolation and Identification of Entomopathogenic Fungi and Their Evaluation Against Tribolium confusum, Sitophilus zeamais, and Rhyzopertha dominica in Stored Maize. Journal of Pest Science, 86: 217-226.
- Batta YA 2008. Control of Main Stored-Grain Insects with New Formulations of Entomopathogenic Fungi in Diatomaceous Earth Dusts. International Journal of Food and Engineering, 4: 1-16.
- Er MK, Tunaz H, Ücük C, Barış C, Işıkber AA 2016. Occurrence of Entomopathogenic Fungi on Insect Pests of Stored Wheat and Maize in Central and South Anatolia in Turkey. Turkish Journal of Entomology, 40: 249-263.
- Kavallieratos NG, Athannassiou CG, Michalaki MP, Batta YA, Rigatos HA, Pashalidou FG, Balotis GN, Tomanovic Z, Vayias BJ 2006. Effect of the Combined Use of Metarhizium anisopliae (Metschinkoff) Sorokin and Diatomaceous Earth for the Control of Three Stored-Product Beetle Species. Crop Protection, 25: 1087-1094.
- Khashaveh A, Ghosta Y, Safaralizadeh MH, Ziaee M 2011. The Use of Entomopathogenic Fungus, Beauveria bassiana (Bals.) Vuill. in Assays with Storage Grain Beetles. Journal of Agricultural Science and Technology, 13: 35-43.
- Lord JC 2007. Enhanced Efficacy of Beauveria bassiana For Red Flour beetle With Reduced Moisture. Journal of Economic Entomology, 100: 1071-1074.
- Lord JC 2009. Efficacy of Beauveria bassiana for Control of Tribolium castaneum with Reduced Oxygen and Increased Carbon Dioxide. J. Appl. Entomol., 133: 101-107.
- Lord JC 2010. Dietary Stress Increases The Susceptibility of Tribolium castaneum to Beauveria bassiana. Journal of Economic Entomology, 103: 1542-1546.
- Michalaki MP, Athanassiou CG, Kavallieratos NG, Batta YA, Balotis GN 2006. Effectiveness of Metarhizium anisopliae (Metschinkoff) Sorokin Applied Alone or in Combination With Diatomaceous Earth Against Tribolium confusum du Val. Larvae: Influence of Temperature, Relative Humidity and Type of Commodity. Crop Protection, 25: 418-425.
- Michalaki MP, Athanassiou CG, Steenberg T, Buchelos C Th 2007. Effect of Paecilomyces fumosoroseus (Wise) Brown and Smith (Ascomycota: Hypocreales) Alone or in Combination With Diatomaceous Earth Against Tribolium confusum Jacquelin du Val (Coleoptera: Tenebrionidae) and Ephestia kuehniella Zeller (Lepidoptera: Pyralidae). Biological Control, 40: 280-286.
- Padin S, Bello GD, Fabrizio M 2002. Grain Loss Caused by Tribolium castaneum, Sitophilus oryzae and Acanthoscelides obtectus in Stored Durum Wheat and Beans Treated With Beauveria bassiana. Journal of Stored Products Research, 38: 69-74.
- Rice WC, Cogburn RR 1999. Activity of the Entomopathogenic Fungus Beauveria bassiana (Deuteromycota: Hyphomycetes) Against Three Coleoptera Pest of Stored Grain. Journal of Economic Entomology, 92(3): 691-694.
- Shafighi Y, Ziaee M, Ghosta Y 2014. Diatomaceous Earth Used against Insect Pests, Applied Alone or in Combination with Metarhizium anisopliae and Beauveria bassiana. Journal of Plant Protection Research, 54 (1): 62-66.
- Stephou VK, Tjamos SE, Paplomatas EJ, Athanassiou CG 2012. Transformation and Attachment of Beauveria bassiana Conidia on The Cuticle of Tribolium confusum and Sitophilus oryzae in Conjunction with Diatomaceous Earth. Journal Pest Science, 85: 387-394.
- Sugandi R, Awaknavar JS 2014. The Pathogenicity of Entomopathogenic Fungus, Metarhizium anisopliae (Metschinkoff) Sorokin against Three Major Storage Grain Beetles. Journal of Experimental Zoology, 17 (2): 775-780.
- Wakil W, Ghazanfar MU, Yasin M 2014. Naturally Occurring Entomopathogenic Fungi Infecting Stored Grain Insect Species in Punjab, Pakistan. Journal of Insect Science, 14 (182), DOI: 10.1093/jisesa/ieu044.
- Wakil W, Schmitt T 2014. Field Trials on the Efficacy of Beauveria bassiana, Diatomaceous Earth and Imidacloprid for the Protection of Wheat Grains From Four Major Stored Grain Insect Pests. Journal of Stored Products Research, 64: 160-167.
- Winston PW, Bates DH 1960. Saturated Solutions for The Control of Humidity in Biological Research. Ecology, 41:232-237.
- Zamani Z, Aminaee MM, Khaniki GB 2013. Introduction of Beauveria bassiana as a Biological Control Agent for Tribolium castaneum in Kerman Province. Archives of Phytopathology and Plant Protection, 46: 2235-2243.
Beauveria bassiana’nın Tribolium confusum (Coleoptera: Tenebrionidae) F1 Larva Sayısına ve Larva Ölüm Oranına Etkisi
Abstract
Bu çalışmada, Beauveria bassiana enfeksiyonu
neticesinde larva ölümleri ve B. bassiana
sporlarının bulunması durumunda yeni nesil larva sayıları belirlenmiştir.
Larvalar spor ile muamele edilmiş buğdayda bekletilmiş ve ölümler
kaydedilmiştir. Uzun süreli denemede, larva sayısı ile ölüm oranları 3 ay sonra
tespit edilmiştir. Yedi günde larva ölümü 250ppm’de %55 ve daha yüksek
konsantrasyonlarda %75’in üstünde bulunmuştur. Uygulamalardaki ölüm 14 günde
%100’e ulaşmıştır. Erginlerin 14 ve 28 gün sporlu buğdayda bekletilmesi
sonucunda 6.3 ve 12 larva belirlenirken kontrolde bu sayı sırası ile 1 ve 6.3
olmuştur. Uzun süreli denemede, kontroldeki, 500ppm ve 1000ppm
uygulamalarındaki larva sayıları sırası ile 10.3, 42.0 ve 260.3 olarak tespit
edilmiştir. 500ppm’de 97.4% ve 1000ppm’de %100 larva ölümü kayıt edilmiştir.
Sonuçlar, spor konsantrasyonu artışı ile birlikte B. bassiana uygulamasının T.
confusum larva sayısında artışa neden olduğunu; ve ancak konsantrasyon ve
süreye bağlı olmakla birlikte B. bassiana
uygulamasının yüksek larva ölümlerini sağladığını göstermiş olup, B. bassiana uygulamasının T. confusum popülasyonları ile
mücadelede rol alabileceğini işaret etmektedir.
Keywords
Tribolium confusum Biyolojik mücadele Mikrobiyal mücadele Depo zararlısı Beauveria bassiana
Project Number
Project No: 2016/5-42 YLS
References
- Akbar W, Lord JC, Nechols JR, Howard RW 2004. Diatomaceous Earth Increases the Efficacy of Beauveria bassiana Against Tribolium castaneum Larvae and Increases Conidia Attachment. Journal of Economic Entomology, 97: 273-280.
- Akbar W, Lord JC, Nechols JR, Loughin TM 2005. Efficacy of Beauveria bassiana for Red Flour Beetle When Applied with Plant Essential Oils or in Mineral Oil and Organosilicone Carriers. Journal of Economic Entomology, 98: 683-688.
- Barış C 2016. The Use of Some Cereals on The Production of Beauveria bassiana By Solid State Fermentation Technique. University of Kahramanmaraş Sütçü İmam, Department of Plant Protection, M.Sc. thesis, Turkey.
- Barra R, Rosso L, Nesci A, Etcheverry M 2013. Isolation and Identification of Entomopathogenic Fungi and Their Evaluation Against Tribolium confusum, Sitophilus zeamais, and Rhyzopertha dominica in Stored Maize. Journal of Pest Science, 86: 217-226.
- Batta YA 2008. Control of Main Stored-Grain Insects with New Formulations of Entomopathogenic Fungi in Diatomaceous Earth Dusts. International Journal of Food and Engineering, 4: 1-16.
- Er MK, Tunaz H, Ücük C, Barış C, Işıkber AA 2016. Occurrence of Entomopathogenic Fungi on Insect Pests of Stored Wheat and Maize in Central and South Anatolia in Turkey. Turkish Journal of Entomology, 40: 249-263.
- Kavallieratos NG, Athannassiou CG, Michalaki MP, Batta YA, Rigatos HA, Pashalidou FG, Balotis GN, Tomanovic Z, Vayias BJ 2006. Effect of the Combined Use of Metarhizium anisopliae (Metschinkoff) Sorokin and Diatomaceous Earth for the Control of Three Stored-Product Beetle Species. Crop Protection, 25: 1087-1094.
- Khashaveh A, Ghosta Y, Safaralizadeh MH, Ziaee M 2011. The Use of Entomopathogenic Fungus, Beauveria bassiana (Bals.) Vuill. in Assays with Storage Grain Beetles. Journal of Agricultural Science and Technology, 13: 35-43.
- Lord JC 2007. Enhanced Efficacy of Beauveria bassiana For Red Flour beetle With Reduced Moisture. Journal of Economic Entomology, 100: 1071-1074.
- Lord JC 2009. Efficacy of Beauveria bassiana for Control of Tribolium castaneum with Reduced Oxygen and Increased Carbon Dioxide. J. Appl. Entomol., 133: 101-107.
- Lord JC 2010. Dietary Stress Increases The Susceptibility of Tribolium castaneum to Beauveria bassiana. Journal of Economic Entomology, 103: 1542-1546.
- Michalaki MP, Athanassiou CG, Kavallieratos NG, Batta YA, Balotis GN 2006. Effectiveness of Metarhizium anisopliae (Metschinkoff) Sorokin Applied Alone or in Combination With Diatomaceous Earth Against Tribolium confusum du Val. Larvae: Influence of Temperature, Relative Humidity and Type of Commodity. Crop Protection, 25: 418-425.
- Michalaki MP, Athanassiou CG, Steenberg T, Buchelos C Th 2007. Effect of Paecilomyces fumosoroseus (Wise) Brown and Smith (Ascomycota: Hypocreales) Alone or in Combination With Diatomaceous Earth Against Tribolium confusum Jacquelin du Val (Coleoptera: Tenebrionidae) and Ephestia kuehniella Zeller (Lepidoptera: Pyralidae). Biological Control, 40: 280-286.
- Padin S, Bello GD, Fabrizio M 2002. Grain Loss Caused by Tribolium castaneum, Sitophilus oryzae and Acanthoscelides obtectus in Stored Durum Wheat and Beans Treated With Beauveria bassiana. Journal of Stored Products Research, 38: 69-74.
- Rice WC, Cogburn RR 1999. Activity of the Entomopathogenic Fungus Beauveria bassiana (Deuteromycota: Hyphomycetes) Against Three Coleoptera Pest of Stored Grain. Journal of Economic Entomology, 92(3): 691-694.
- Shafighi Y, Ziaee M, Ghosta Y 2014. Diatomaceous Earth Used against Insect Pests, Applied Alone or in Combination with Metarhizium anisopliae and Beauveria bassiana. Journal of Plant Protection Research, 54 (1): 62-66.
- Stephou VK, Tjamos SE, Paplomatas EJ, Athanassiou CG 2012. Transformation and Attachment of Beauveria bassiana Conidia on The Cuticle of Tribolium confusum and Sitophilus oryzae in Conjunction with Diatomaceous Earth. Journal Pest Science, 85: 387-394.
- Sugandi R, Awaknavar JS 2014. The Pathogenicity of Entomopathogenic Fungus, Metarhizium anisopliae (Metschinkoff) Sorokin against Three Major Storage Grain Beetles. Journal of Experimental Zoology, 17 (2): 775-780.
- Wakil W, Ghazanfar MU, Yasin M 2014. Naturally Occurring Entomopathogenic Fungi Infecting Stored Grain Insect Species in Punjab, Pakistan. Journal of Insect Science, 14 (182), DOI: 10.1093/jisesa/ieu044.
- Wakil W, Schmitt T 2014. Field Trials on the Efficacy of Beauveria bassiana, Diatomaceous Earth and Imidacloprid for the Protection of Wheat Grains From Four Major Stored Grain Insect Pests. Journal of Stored Products Research, 64: 160-167.
- Winston PW, Bates DH 1960. Saturated Solutions for The Control of Humidity in Biological Research. Ecology, 41:232-237.
- Zamani Z, Aminaee MM, Khaniki GB 2013. Introduction of Beauveria bassiana as a Biological Control Agent for Tribolium castaneum in Kerman Province. Archives of Phytopathology and Plant Protection, 46: 2235-2243.
Details
| Primary Language | English |
|---|---|
| Subjects | Agricultural, Veterinary and Food Sciences |
| Journal Section | RESEARCH ARTICLE |
| Authors | |
| Project Number | Project No: 2016/5-42 YLS |
| Publication Date | December 31, 2019 |
| Submission Date | March 19, 2019 |
| Acceptance Date | May 16, 2019 |
| Published in Issue | Year 2019Volume: 22 Issue: 6 |
Cite
Cited By
The effect of two isolates of Beauveria bassiana (Bals.) Vull. on the larvae of confused flour beetle [Tribolium confusum du Val., 1863 (Coleoptera: Tenebrionidae)]
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