Year 2019, Volume 22 , Issue , Pages 24 - 29 2019-10-31

İnsan Kanser Hücrelerinde Astragalus elongatus subsp. nucleiferus’ un Antikanser, Antiproliferatif ve Laktat Dehidrojenaz Enzim Aktiviteleri
Anticancer, Antiproliferative and Lactate Dehydrogenase Enzyme Activities of Astragalus elongatus subsp. nucleiferus on Human Cancer Cells

Nazım ŞEKEROĞLU [1] , Sevgi GEZİCİ [2] , Ceren SERİN TANRIÖVER [3] , Fatih YAYLA [4]


Bu araştırmada, Astragallus elongatus subsp. nucleiferus’ un toprak üstü kısmı ve köklerinden elde edilen etanol ve su özütlerinin potansiyel antikanser ve antiproliferatif aktivitelerinin araştırılması amaçlanmıştır. Bu bağlamda, bitki özütlerinin A549, H1299, C6 insan kanser hücreleri ve kanserli olmayan HUVEC hücrelerine karşı sitotoksisite ve hücre proliferasyon potansiyellerini araştırmak için; MTT, tripan mavisi ve LDH enzim aktivite analizleri yapılmıştır. Özütlerin kanser hücrelerine karşı sitotoksisitelerine göre, özütlerin çeşitli konsantrasyonları in vitro analizler için belirlenmiştir. MTT ve tripan mavisi deneylerinin bir sonucu olarak, test edilen insan akciğer ve beyin karsinomu hücrelerinde doza bağlı antikanser ve antiproliferatif etkiler gözlenmiştir. Bitkinin kökünden elde edilen su özütleri, 5.81±0.46 ile 18.24±0.12μg / mL arasında değişen IC50 değerleriyle, toprak üstü özütlerinden daha yüksek bir antikanser aktivite göstermiştir. Artan özüt konsantrasyonunun, kanser hücrelerinin büyüme oranını düşürmesine yol açmasının yanı sıra; test edilen insan kanser hücrelerinde apoptoz gözlenmiştir. Antikanser ve sitotoksisite sonuçlarının aksine, kök etanol özütleri doza bağlı olarak en yüksek apoptoz seviyesine neden olmuştur. LDH aktivitesi ile ilgili olarak; toprak üstü kısımdan ve köklerden elde edilen bitki özütlerinin H1299 hücrelerine karşı en yüksek LDH aktivitesi gösterdiği ve bunu A549 hücrelerinin izlediği ortaya konulmuştur, ki bu sonuçlar MTT ve tripan mavisi analizlerinde gözlenen duruma benzer bir aktivite profili göstermiştir. Bu çalışmadan elde edilen sonuçlar; bitki köklerinin, kanser hücrelerinde hücre büyümesini konsantrasyon ve zamana bağlı olarak önleyebildiğini göstermiştir.

In this study, potential anticancer and antiproliferative activities of ethanol and water extracts from aerial parts and roots of Astragallus elongatus subsp. nucleiferus were evaluated. In this context, MTT, trypan blue, and LDH enzyme activity assays were performed to determine cytotoxicity and cell proliferation potentials of the plant extracts against human A549, H1299, C6 cancer cells, and non-tumorous HUVECs. Based on cytotoxicity against the cancer cells, the various concentrations of the extracts were selected for in vitro assays. As a result of MTT and trypan blue assays, dose-dependent anticancer and antiproliferative effects were observed on tested human lung and brain carcinoma cells. The water extracts obtained from root of the plant exerted higher anticancer activity with IC50 values ranging from 5.81±0.46 to 18.24±0.12µg/mL than the aerial parts of the extracts. Besides increasing extract concentration lead to decrease the growth rate of the cancer cells, apoptosis was observed in the tested human malignant cells. In contrary anticancer and cytotoxicity results, the ethanol extract of the root was also caused to the highest apoptosis level in a dose dependently. Regarding LDH activity, the plant extracts obtained from aerial parts and roots were demonstrated the highest LDH activity towards H1299 cells, which was followed by A549 cells. The results were found a similar activity profile as observed in MTT and trypan blue assays. These results obtained from this work showed that the roots of the plant are able to inhibit cell growth in cancer cells in a time and concentration dependently. 

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Primary Language en
Subjects Biology
Journal Section RESEARCH ARTICLE
Authors

Orcid: 0000-0002-0630-0106
Author: Nazım ŞEKEROĞLU (Primary Author)
Institution: KILIS 7 ARALIK UNIVERSITY
Country: Turkey


Orcid: 0000-0002-4856-0221
Author: Sevgi GEZİCİ
Institution: KILIS 7 ARALIK UNIVERSITY
Country: Turkey


Orcid: 0000-0002-4974-6669
Author: Ceren SERİN TANRIÖVER
Institution: KILIS 7 ARALIK UNIVERSITY
Country: Turkey


Orcid: 0000-0002-6490-6288
Author: Fatih YAYLA
Institution: GAZİANTEP UNIVERSITY ARTS AND SCIENCE FACULTY
Country: Turkey


Supporting Institution Scientific Research Project Unit of Kilis 7 Aralık University
Project Number Project code no: 1859LTP1
Thanks Authors would like to thank Gaziantep University, Medical Biology Department, Genetics Research Laboratory for their technical support.
Dates

Application Date : May 23, 2019
Acceptance Date : July 3, 2019
Publication Date : October 31, 2019

APA Şekeroğlu, N , Gezi̇ci̇, S , Seri̇n Tanrıöver, C , Yayla, F . (2019). Anticancer, Antiproliferative and Lactate Dehydrogenase Enzyme Activities of Astragalus elongatus subsp. nucleiferus on Human Cancer Cells . Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , Vol 22 (Suppl 1) , 24-29 . DOI: 10.18016/ksutarimdoga.v22i49073.569493