Zeatin ve Farklı Oksin Kombinasyonlarının Önemli Tıbbi Bitki Limnophila aromatica (Lamk.) Merr.’nın İn Vitro Mikroçoğaltımı Üzerine Etkisi
Abstract
Limnophila
aromatica (Lamk.) Merr. içerdikleri kimyasal ve farmakolojik
özellikleri ile geleneksel tıp sistemindeki en önemli bitki türlerinden biri
olarak kabul edilir. Bu çalışma, tıbbi bitki L. aromatica'nın doku kültürü teknikleriyle hızlı ve verimli bir
şekilde üretilmesi üzerine Zeatin (ZEA) ve oksin kombinasyonlarının etkilerini
sunmaktadır. L. aromatica'nın yaprak
eksplantları 0.10-1.60 mg L-1 ZEA ve 0.10 mg L-1 indol-3-asetik
asit (IAA), indol-3-butirik asit (IBA) ve naftalen asetik asit (NAA) eklenmiş
Murashige ve Skoog (MS) besin ortamında altı hafta boyunca kültüre alınmıştır. İlk
sürgün çıkışları 14. günde ZEA+IBA içeren kültür ortamında gözlenmiştir. Sürgün rejenerasyon yüzdeleri ZEA+IAA kombinasyonunu
içeren MS besin ortamında %72,22-100,00 arasında, ZEA+IBA ve ZEA+NAA içeren MS besin
ortamlarında %83,33-100,00 arasında değişmiştir. Hormon uygulamaları
kıyaslandığında eksplant başına maksimum sürgün sayısı 0,10 mg L-1
ZEA + 0,10 mg L-1 IBA içeren MS besin ortamında elde edilmiştir (25,29 adet),
ardından 0,10 mg L-1 ZEA + 0,10 mg L-1 IBA içeren MS
besin ortamında tespit edilmiştir (23,72 adet). Sürgün uzunlukları ZEA+IAA
uygulamalarında 1,24-1,41 cm, ZEA+IBA uygulamalarında 1,28-1,47 cm ve ZEA+NAA
uygulamalarında 1,16-1,34 cm olarak elde edilmiştir. En uzun sürgünler
ZEA+IBA’nın kullanıldığı kültür ortamlarında kaydedilmiştir. Rejenere
sürgünler, 0.25 mg L-1 IBA içeren MS ortamında köklendirildi ve
köklenmiş bitkiler akvaryuma başarıyla alıştırılmıştır.
References
- Ahmad N, Faisal M, Anisa M, Aref IM 2010. In vitro Callus Induction and Plant Regeneration from Leaf Explants of Ruta graveolens L. South African Journal of Botany, 76(3): 597-600.
- Anand S 2010. Various Approaches for Secondary Metabolite Production Through Plant Tissue Culture. Pharmacia 1: 1-7.
- Anand SP, Velmurugan G, Doss A 2015. In-vitro Direct Regeneration from Nodal Explants of Toddalia asiatica (L.) Lam. Asian Journal of Plant Science and Research, 5(1): 49-53.
- Ayyavu M, Devarajan T, Gabriel M. 2012. Rapid In Vitro Plant Regeneration From Leaf Explants of Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze. Biological Research, 45: 131-136.
- Chaturvedi HC, Jain M, Kidwai NR 2007. Cloning of Medicinal Plants Through Tissue Culture-A Review. Indian Journal of Experimental Biology, 45: 937-948.
- Davies PJ 2010. The Plant Hormones: Their Nature, Occurrence, and Functions. (In: Davies P.J. (eds) Plant Hormones. Springer, Dordrecht) 1-15.
- Diasa MI, Sousa MJ, Alves RC, Ferreira ICFR 2016. Exploring Plant Tissue Culture to Improve the Production of Phenolic Compounds: A Review. Industrial Crops and Products, 82: 9-22.
- Dogan M 2018. In Vitro Micropropagation from Nodal Explants of the Medicinal Plant Lysimachia nummularia L.. KSU Journal of Agriculture and Nature, 21(6): 875-881.
- Dogan M 2019. Multiple Shoot Regeneration Via Indirect Organogenesis from Shoot Tip and Nodal Meristem Explants of Ceratophyllum demersum L. Journal of Animal and Plant Sciences, 29(2): 568-577.
- El-Sherif NA 2018. Impact of Plant Tissue Culture on Agricultural Sustainability. (In: Negm A., Abu-hashim M. (eds) Sustainability of Agricultural Environment in Egypt: Part II. The Handbook of Environmental Chemistry,Springer, Cham.) 93-107.
- Emsen B, Dogan M 2018. Evaluation of Antioxidant Activity of In Vitro Propagated Medicinal Ceratophyllum demersum L. Extracts. Acta Scientiarum Polonorum-Hortorum Cultus, 17(1): 23-33.
- Enyew M, Feyissa T, 2019. In Vitro Shoot Regeneration From Leaf Explants of Echinops kebericho: An Endangered Endemic Medicinal Plant. Plant Biosystems, 153(2): 199-204.
- Faria DV, Simao MJ, Cipriano R, Werner ET, Soares TCB, Aoyama EM, Lima-Gontijo ABP 2018. In vitro Morphogenesis and Micropropagation of Aechmea ramosa var. ramosa Mart. ex Schult. f. (Bromeliaceae) from Leaf Explants. In Vitro Cellular & Developmental Biology-Plant, 54(5): 530-536.
- Haddadi F, Abd Aziz M, Kamaladini H, Rayanfar SA 2013. Thidiazuron- and Zeatin-Induced High-Frequency Shoot Regeneration from Leaf and Shoot-Tip Explants of Strawberry. Horttechnology, 23(3): 276-281.
- Imtiaz M, Khattak AM, Khan MA, Jalal F, Hussain S, Said F, Bo H, 2019. Rapid In-Vitro Propagation of Chrysanthemum morifolıum Through Shoot Bud Explants. Pakistan Journal of Botany, 51(3): 1093-1098.
- Karuppusamy S 2009. A Review On Trends İn Production Of Secondary Metabolitesfrom Higher Plants by In Vitro Tissue, Organ And Cell Cultures. Journal of. Medicinal Plants Research, 3: 1222-1239.
- Kukongviriyapan U, Luangaram S, Leekhaosoong S, Kukongviriyapan V, Preeprame S, 2007. Antioxidant and Vascular Protective Activities of Cratoxylum formosum, Syzygium gratum and Limnophila aromatica. Biological and Pharmaceutical Bulletin, 30(4): 661-666.
- Kumar N, Reddy MP 2010. Plant Regeneration Through the Direct İnduction of Shoot Buds from Petiole Explants of Jatropha curcas: a Biofuel Plant, Annals of Applied Biology, 156(3): 367-375.
- Maheshwari P, Kumar A 2006. Organogenesis, Shoot Regeneration, And Flowering Response of Vernonia cinerea to Different Auxin And Cytokinin Combinations. In Vitro Cell Dev Biol. 42: 589-595.
- Masekesa TR, Gasura E, Ngadze E, Icishahayo D, Kujeke GT, Chidzwondo F, Robertson I 2016. Efficacy of Zeatin, Kinetin and Thidiazuron in induction of adventitious root and shoot from petiole explants of sweetpotato cv. Brondal. South African Journal of Botany, 104: 1-5.
- Murashige T, Skoog F 1962. A Revised Medium For Rapid Growth And Bioassays With Tobacco Tissue Cultures. Physiological Plantarum, 15: 473-497.
- Roberto T, Francesca M 2011. Sustainable Sourcing Of Natural Food Ingredientsby Plant Cell Cultures. Agro Food Industry Hi Tech, 22: 26-28.
- Sharma S, Rathi N, Kamal B, Pundir D, Kaur B, Arya S 2010. Conservation of Biodiversity of Highly Important Medicinal Plants of India Through Tissue Culture Technology- A Review. Agriculture and Biology Journal of North America, 1(5): 827-833.
- Sidhu Y 2010. In Vitro Micropropagation Of Medicinal Plants By Tissue Culture. The Plymouth Student Scientist, 4(1): 432-449.
- Sivanesan I, Jeong BR 2007. Direct Shoot Regeneration From Nodal Explants of Sida cordifolia Linn. In Vitro Cell Dev Biol. 43: 436-441.
- Snedecor GW, Cochran WG 1997. Statistical Methods. The Iowa State University Press, Iowa, USA.
- Sun QR, Sun HY, Bell RL, Guan QZ 2018. Plant Regeneration From In Vitro Leaf Explants of Prunus pseudocerasus Lindl. Propagation of Ornamental Plants, 18(1): 3-11.
- Tafvizi F, Farahanei F, Sheidai M, Nejadsattari T 2009. Effects of Zeatin And Activated Charcoal in Proliferation of Shoots And Direct Regeneration In Cotton (Gossypium hirsutum L.). African Journal of Biotechnology, 8(22): 6220-6227.
- Zhou LG, Wu JY 2006. Development and Application of Medicinal Plant Tissue Cultures for Production of Drugs and Herbal Medicinals in China. Natural Product Reports, 23: 789-810
The Effect of Zeatin and Different Auxin Combinations on In Vitro Micropropagation of Limnophila aromatica (Lamk.) Merr., an Important Medicinal Plant
Abstract
This study presents the
effects of Zeatin (ZEA) and auxin combinations on the fast and efficient
production of medicinal plant Limnophila
aromatica (Lamk.) Merr. by tissue culture techniques. The leaf explants of L. aromatica were cultured on Murashige
and Skoog (MS) nutrient medium supplemented with 0.10-1.60 mg L-1
ZEA and 0.10 mg L-1 indole-3-acetic acid (IAA), indole-3-butyric
acid (IBA) and naphthaleneacetic acid (NAA) for six weeks. The maximum number
of shoots per explant compared to hormone applications were obtained in MS
nutrient medium containing 0.10 mg L-1 ZEA + 0.10 mg L-1 IBA (25.29), followed by in the MS nutrient
medium containing 0.10 mg L-1 ZEA + 0.10 mg L-1 IBA
(23.72). The shoot lengths were obtained as 1.24-1.41 cm in ZEA + IAA
applications, 1.28-1.47 cm in ZEA + IBA applications and 1.16-1.34 cm in ZEA +
NAA applications. The longest shoots were recorded in culture media using ZEA +
IBA. The regenerated shoots were rooted on MS medium containing 0.25 mg L-1
IBA and then the rooted plantlets were successfully acclimatized in an
aquarium. As a result, the most suitable hormone combination for
in vitro production was determined as
0.10 mg L-1 ZEA + 0.10 mg L-1 IBA. This production
protocol may be useful for commercially large scale production.
References
- Ahmad N, Faisal M, Anisa M, Aref IM 2010. In vitro Callus Induction and Plant Regeneration from Leaf Explants of Ruta graveolens L. South African Journal of Botany, 76(3): 597-600.
- Anand S 2010. Various Approaches for Secondary Metabolite Production Through Plant Tissue Culture. Pharmacia 1: 1-7.
- Anand SP, Velmurugan G, Doss A 2015. In-vitro Direct Regeneration from Nodal Explants of Toddalia asiatica (L.) Lam. Asian Journal of Plant Science and Research, 5(1): 49-53.
- Ayyavu M, Devarajan T, Gabriel M. 2012. Rapid In Vitro Plant Regeneration From Leaf Explants of Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze. Biological Research, 45: 131-136.
- Chaturvedi HC, Jain M, Kidwai NR 2007. Cloning of Medicinal Plants Through Tissue Culture-A Review. Indian Journal of Experimental Biology, 45: 937-948.
- Davies PJ 2010. The Plant Hormones: Their Nature, Occurrence, and Functions. (In: Davies P.J. (eds) Plant Hormones. Springer, Dordrecht) 1-15.
- Diasa MI, Sousa MJ, Alves RC, Ferreira ICFR 2016. Exploring Plant Tissue Culture to Improve the Production of Phenolic Compounds: A Review. Industrial Crops and Products, 82: 9-22.
- Dogan M 2018. In Vitro Micropropagation from Nodal Explants of the Medicinal Plant Lysimachia nummularia L.. KSU Journal of Agriculture and Nature, 21(6): 875-881.
- Dogan M 2019. Multiple Shoot Regeneration Via Indirect Organogenesis from Shoot Tip and Nodal Meristem Explants of Ceratophyllum demersum L. Journal of Animal and Plant Sciences, 29(2): 568-577.
- El-Sherif NA 2018. Impact of Plant Tissue Culture on Agricultural Sustainability. (In: Negm A., Abu-hashim M. (eds) Sustainability of Agricultural Environment in Egypt: Part II. The Handbook of Environmental Chemistry,Springer, Cham.) 93-107.
- Emsen B, Dogan M 2018. Evaluation of Antioxidant Activity of In Vitro Propagated Medicinal Ceratophyllum demersum L. Extracts. Acta Scientiarum Polonorum-Hortorum Cultus, 17(1): 23-33.
- Enyew M, Feyissa T, 2019. In Vitro Shoot Regeneration From Leaf Explants of Echinops kebericho: An Endangered Endemic Medicinal Plant. Plant Biosystems, 153(2): 199-204.
- Faria DV, Simao MJ, Cipriano R, Werner ET, Soares TCB, Aoyama EM, Lima-Gontijo ABP 2018. In vitro Morphogenesis and Micropropagation of Aechmea ramosa var. ramosa Mart. ex Schult. f. (Bromeliaceae) from Leaf Explants. In Vitro Cellular & Developmental Biology-Plant, 54(5): 530-536.
- Haddadi F, Abd Aziz M, Kamaladini H, Rayanfar SA 2013. Thidiazuron- and Zeatin-Induced High-Frequency Shoot Regeneration from Leaf and Shoot-Tip Explants of Strawberry. Horttechnology, 23(3): 276-281.
- Imtiaz M, Khattak AM, Khan MA, Jalal F, Hussain S, Said F, Bo H, 2019. Rapid In-Vitro Propagation of Chrysanthemum morifolıum Through Shoot Bud Explants. Pakistan Journal of Botany, 51(3): 1093-1098.
- Karuppusamy S 2009. A Review On Trends İn Production Of Secondary Metabolitesfrom Higher Plants by In Vitro Tissue, Organ And Cell Cultures. Journal of. Medicinal Plants Research, 3: 1222-1239.
- Kukongviriyapan U, Luangaram S, Leekhaosoong S, Kukongviriyapan V, Preeprame S, 2007. Antioxidant and Vascular Protective Activities of Cratoxylum formosum, Syzygium gratum and Limnophila aromatica. Biological and Pharmaceutical Bulletin, 30(4): 661-666.
- Kumar N, Reddy MP 2010. Plant Regeneration Through the Direct İnduction of Shoot Buds from Petiole Explants of Jatropha curcas: a Biofuel Plant, Annals of Applied Biology, 156(3): 367-375.
- Maheshwari P, Kumar A 2006. Organogenesis, Shoot Regeneration, And Flowering Response of Vernonia cinerea to Different Auxin And Cytokinin Combinations. In Vitro Cell Dev Biol. 42: 589-595.
- Masekesa TR, Gasura E, Ngadze E, Icishahayo D, Kujeke GT, Chidzwondo F, Robertson I 2016. Efficacy of Zeatin, Kinetin and Thidiazuron in induction of adventitious root and shoot from petiole explants of sweetpotato cv. Brondal. South African Journal of Botany, 104: 1-5.
- Murashige T, Skoog F 1962. A Revised Medium For Rapid Growth And Bioassays With Tobacco Tissue Cultures. Physiological Plantarum, 15: 473-497.
- Roberto T, Francesca M 2011. Sustainable Sourcing Of Natural Food Ingredientsby Plant Cell Cultures. Agro Food Industry Hi Tech, 22: 26-28.
- Sharma S, Rathi N, Kamal B, Pundir D, Kaur B, Arya S 2010. Conservation of Biodiversity of Highly Important Medicinal Plants of India Through Tissue Culture Technology- A Review. Agriculture and Biology Journal of North America, 1(5): 827-833.
- Sidhu Y 2010. In Vitro Micropropagation Of Medicinal Plants By Tissue Culture. The Plymouth Student Scientist, 4(1): 432-449.
- Sivanesan I, Jeong BR 2007. Direct Shoot Regeneration From Nodal Explants of Sida cordifolia Linn. In Vitro Cell Dev Biol. 43: 436-441.
- Snedecor GW, Cochran WG 1997. Statistical Methods. The Iowa State University Press, Iowa, USA.
- Sun QR, Sun HY, Bell RL, Guan QZ 2018. Plant Regeneration From In Vitro Leaf Explants of Prunus pseudocerasus Lindl. Propagation of Ornamental Plants, 18(1): 3-11.
- Tafvizi F, Farahanei F, Sheidai M, Nejadsattari T 2009. Effects of Zeatin And Activated Charcoal in Proliferation of Shoots And Direct Regeneration In Cotton (Gossypium hirsutum L.). African Journal of Biotechnology, 8(22): 6220-6227.
- Zhou LG, Wu JY 2006. Development and Application of Medicinal Plant Tissue Cultures for Production of Drugs and Herbal Medicinals in China. Natural Product Reports, 23: 789-810
Details
| Primary Language | Turkish |
|---|---|
| Journal Section | RESEARCH ARTICLE |
| Authors | |
| Publication Date | December 31, 2019 |
| Submission Date | April 19, 2019 |
| Acceptance Date | July 17, 2019 |
| Published in Issue | Year 2019Cilt 22 (Ek Sayı 2) |
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