Year 2019, Volume 22 , Issue , Pages 374 - 381 2019-12-31

Anticancer, Antiproliferative and Lactate Dehydrogenase Enzyme Activities of Different Parts of Acorn (Quercus coccifera L.) against A549, MCF-7 and HeLa Human Cancer Cells
Meşe Palamudunun (Quercus coccifera L.) Farklı Kısımlarının A549, MCF-7 ve HeLa İnsan Kanser Hücrelerine Karşı Antikanser, Antiproliferatif ve Laktat Dehidrojenaz Enzim Aktiviteleri

Sevgi GEZİCİ [1]


In this study, potential anticancer antiproliferative and necrotic capacities of the extracts obtained from shell, cup and shelled acorn parts of acorn (Q. coccifera) were investigated against A549, MCF-7, HeLa cancer cells and non-tumorous HUVECs. As a result of the analysis, it was proved that the different parts obtained from the acorns have anticancer and antiproliferative effects, even at the lowest concentration. The ethanol extracts obtained from the cup parts of acorn showed higher anticancer activity with IC50 values ranging from 5.04±0.02 to 18.04±0.16 µg/mL than the extracts from other parts. The highest rate of cytotoxic activity was observed against MCF-7 cells, whilst the lowest cytotoxic activity was observed against A549 cells. In contrast to anticancer and antiproliferative activity results, the highest LDH activity was determined in HeLa cells. In accordance with the MTT and trypan blue assay results, the highest LDH release capacity was found in the ethanol extracts obtained from the cup part. This is an original study that reveals acorn has the potential to inhibit cell growth in the cancer cells depending on concentration and time.

Bu çalışmada, Meşe palamudunun (Q. coccifera) dış-kabuk, külah ve iç kısmından elde edilen özütlerin; A549, MCF-7, HeLa kanser hücreleri ve kanserli olmayan HUVEC hücrelerine karşı potansiyel antikanser, antiproliferatif ve nekrotik kapasiteleri araştırılmıştır. Analizler sonucunda, meşe palamudunun farklı kısımlarından elde edilen ekstrelerin en düşük konsantrasyonda dahi antikanser ve antiproliferatif etkiye sahip oldukları ortaya konulmuştur. Palamut meyvesinin külah kısmından elde edilen etanol özütleri, 5.04±0.02 ile 18.04±0.16 µg/mL arasında değişen IC50 değerleriyle, diğer kısımlarından elde edilen özütlerden daha yüksek antikanser aktivite göstermiştir. En yüksek oranda sitotoksik aktivite MCF-7 hücrelerine karşı gözlenirken; en düşük sitotoksik aktivite ise A549 hücrelerinde gözlenmiştir. Antikanser ve antiproliferatif aktivite sonuçlarının aksine, en yüksek LDH aktivitesi HeLa hücrelerinde belirlenmiştir. MTT ve tripan mavisi analiz sonuçlarıyla uyumlu olarak, en yüksek LDH salınım kapasitesi külah kısmından elde etanol özütlerinde saptanmıştır. Bu çalışma; meşe palamudunun, kanser hücrelerinde hücre büyümesini konsantrasyon ve zamana bağlı olarak engelleme potansiyeline sahip olduğunu ortaya koyan, özgün bir çalışmadır.

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Primary Language tr
Subjects Biology
Journal Section RESEARCH ARTICLE
Authors

Orcid: 0000-0002-4856-0221
Author: Sevgi GEZİCİ (Primary Author)
Institution: Moleküler Biyoloji ve Genetik Bölümü, Fen Edebiyat Fakültesi; İleri Teknoloji Araştırma ve Uygulama Merkezi (İTAMER), Genetik Araştırma Laboratuvarı, Kilis 7 Aralık Üniversitesi, Kilis, Türkiye
Country: Turkey


Thanks Teşekkür Bu çalışma, Kilis 7 Aralık Üniversitesi, İleri Teknoloji Araştırma ve Uygulama Merkezi ve Gaziantep Üniversitesi Tıp Fakültesi Laboratuvarlarının alt yapısı kullanılarak gerçekleştirilmiştir. Bitki materyalinin temini ve sistematik olarak tanımlanması Kilis 7 Aralık Üniversitesi Gıda Mühendisliği Bölümü Öğretim Üyesi Prof. Dr. Nazım Şekeroğlu tarafından gerçekleştirilmiştir.
Dates

Application Date : June 21, 2019
Acceptance Date : August 1, 2019
Publication Date : December 31, 2019

APA Gezi̇ci̇, S . (2019). Meşe Palamudunun (Quercus coccifera L.) Farklı Kısımlarının A549, MCF-7 ve HeLa İnsan Kanser Hücrelerine Karşı Antikanser, Antiproliferatif ve Laktat Dehidrojenaz Enzim Aktiviteleri . Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , Cilt 22 (Ek Sayı 2) , 374-381 . DOI: 10.18016/ksutarimdoga.v22i49454.580285