Year 2019, Volume 22 , Issue , Pages 382 - 387 2019-12-31

İncir, Limon, Zeytin ve Turp Ekzozomlarının MCF-7 Hücrelerinde Sitotoksik Etkisinin Araştırılması
Investigation of the Cytotoxicity of Fig, Lemon, Olive, and Turnip Exosomes on MCF-7 Cells

Oğuzhan KARAOSMANOĞLU [1]


Nano-boyutlu yapılarıyla ekzozomlar genetik bilgi akışı, immun sistem düzenlenmesi ve patofizyolojik değişiklikler gibi çok çeşitli hücresel süreçlere dahil olurlar. Ekzozomların, özellikle biyoişaret tanımlanması, etkin ilaç dağıtımı için nanotaşıyıcıların geliştirilmesi ve aşı üretilmesi alanlarında kullanımları hızla artmaktadır. Son yıllarda yenilebilir bitkilerden türevlenen ekzozomlar yüksek antimikrobiyal etkileri, bağırsak kök hücreleri üzerinde düzenleyici etkileri ve antikanser etkileriyle ilgi çekmektedir. Bu çalışmada incir, limon, zetin ve turp türevli ekzozomların sitotoksik etkileri nötral kırmızısı alım testiyle araştırılmıştır. Protein miktarı 100 µg/mL olan limon ve turp ekzozomlarının hücre çoğalmasını engellediği gözlenirken incir ve zeytin türevli ekzozomların MCF-7 çoğalmasını etkilemediği görülmüştür. Turp ekzozomlarının sitotoksistik aktivitesi sonuçları, bu araştırmada orijinal olduğu için, yeni antikanser ajanlarının veya yeni ilaç dağıtım nanotaşıyıcılarının geliştirilmesi için turp ekzozomları kullanımına vurgu yapmanın değerli olabileceği düşünülmüştür.
With their nano-sized structure, exosomes are involved in a wide variety of cellular processes such as genetic information flow, immune system modulations, intercellular communication, and pathophysiological changes. The use of exosomes are exponentially growing particularly in the areas of identification of biomarkers, development of nanocarriers for effective drug delivery, and vaccine production. In recent years, edible plant derived exosomes gained much interest with their strong antimicrobial activities, modulatory activities on the intestinal stem cells, and the anticancer activities. In this study, the cytotoxic effects of fig, lemon, olive and turnip derived exosomes were investigated through the neutral red uptake assay. It was observed that 100 µg/mL protein containing lemon and turnip derived exosomes inhibited the cell proliferation significantly, on the other hand, fig and olive derived exosomes did not alter the proliferation of MCF-7 cells. Since the results for the cytotoxic activity of turnip exosomes are original in this research, it was found worthy to emphasis the utility of turnip exosomes for the development of new anticancer agents or new drug delivery nanocarriers.
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Primary Language en
Subjects Biology
Journal Section RESEARCH ARTICLE
Authors

Orcid: 0000-0003-2028-7339
Author: Oğuzhan KARAOSMANOĞLU (Primary Author)
Institution: KARAMANOGLU MEHMETBEY UNIVERSITY, KAMİL ÖZDAĞ FACULTY OF SCIENCE, DEPARTMENT OF BIOLOGY
Country: Turkey


Dates

Application Date : July 10, 2019
Acceptance Date : August 23, 2019
Publication Date : December 31, 2019

APA Karaosmanoğlu, O . (2019). Investigation of the Cytotoxicity of Fig, Lemon, Olive, and Turnip Exosomes on MCF-7 Cells . Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , Cilt 22 (Ek Sayı 2) , 382-387 . DOI: 10.18016/ksutarimdoga.v22i49454.589845