Fritillaria imperialis needs 2-3 years to regenerate from seeds, which makes its production
quite challenging. In this study, effects of three concentrations of
6-Benzylaminopurine (BAP) (0.00, 0.10 and 0.15 mg/L) and three different
amounts (0.0, 3.0 and 3.5 g/L) of activated carbon (AC) on the growth of F.
imperialis plantlets in vitro were determined. The auxins were at
similar concentration (0.01 mg/L of NAA and 0.01 mg/L of IAA) for all
treatments. It has been found that the size and number of bulblets, as well as
root and shoot numbers in regenerated plantlets were increased significantly
(p<0.05) by adding 3.5 g/L of AC in culture medium supplemented with 0.10
mg/L of BAP. Moreover, addition of AC significantly reduced the length of roots
in all doses studied (p<0.05). The supreme concentration of BAP (0.15 mg/L)
decreased the bulblet number in all treatments, however, when supplemented with
3.5 g/L of AC, it was found to raise all the values measured (p<0.05).
Overall, the best growth of the plant parts from callus were achieved with the
highest concentration of AC and 1.5 mg/L of BAP.
|Journal Section||RESEARCH ARTICLE|
|Supporting Institution||Kahramanmaraş Sütçü İmam Üniversitesi Bilimsel Araştırma Projeleri Birimi|
|Thanks||This work was supported by Scientific Research Projects (BAP) Unit of Kahramanmaras Sutcu Imam University with the project number of 2017/4-28D.|
: September 30, 2019
|APA||Saeed, N , Cömertpay, S . (2020). Effects of 6-benzylaminopurine and Activated Carbon on Indirect Organogenesis of Fritillaria Imperialis . Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 23 (2) , 297-303 . DOI: 10.18016/ksutarimdoga.vi.627056|