Year 2020, Volume 23 , Issue 2, Pages 297 - 303 2020-04-30

Effects of 6-benzylaminopurine and Activated Carbon on Indirect Organogenesis of Fritillaria Imperialis
6-benzilaminopurin ve Aktif Karbonun Fritillaria Imperialis'in Dolaylı Organogenezi Üzerindeki Etkileri

Nigar SAEED [1] , Sabahattin CÖMERTPAY [2]


Fritillaria imperialis needs 2-3 years to regenerate from seeds, which makes its production quite challenging. In this study, effects of three concentrations of 6-Benzylaminopurine (BAP) (0.00, 0.10 and 0.15 mg/L) and three different amounts (0.0, 3.0 and 3.5 g/L) of activated carbon (AC) on the growth of F. imperialis plantlets in vitro were determined. The auxins were at similar concentration (0.01 mg/L of NAA and 0.01 mg/L of IAA) for all treatments. It has been found that the size and number of bulblets, as well as root and shoot numbers in regenerated plantlets were increased significantly (p<0.05) by adding 3.5 g/L of AC in culture medium supplemented with 0.10 mg/L of BAP. Moreover, addition of AC significantly reduced the length of roots in all doses studied (p<0.05). The supreme concentration of BAP (0.15 mg/L) decreased the bulblet number in all treatments, however, when supplemented with 3.5 g/L of AC, it was found to raise all the values measured (p<0.05). Overall, the best growth of the plant parts from callus were achieved with the highest concentration of AC and 1.5 mg/L of BAP.

Fritillaria imperialis türü bitkiler, tohumlarını yenilemek için iki yıldan fazla bir süreye ihtiyacı duymakta ve bu durum üretimlerini zorlaştırmaktadır. Bu çalışmada, üç farklı derişimdeki 6-Benzilaminopurinin (BAP) (0.00, 0.10 ve 0.15 mg/L) ve üç farklı miktardaki (0.0, 3.0 ve 3.5 g/L) aktif karbonun (AC) F. imperialis bitkilerinin büyümesine etkisi in vitro olarak incelenmiştir. Tüm muamele grupları için benzer konsantrasyonda oksin (0.01 mg/L NAA ve 0.01 mg/L IAA) kullanılmıştır. 0.10 mg/L BAP ilave edilmiş kültür ortamlarına 3.5 g/L AC eklenmesinin bitkilerin yumru çap uzunluğu ve sayısının yanında kök ve sürgün sayılarını istatiksel olarak anlamlı bir şekilde (p<0.05) arttırdığı gözlenmiştir. Dahası, AC eklenmesi, çalışılan tüm dozlarda kök uzunluklarını önemli ölçüde azaltmıştır (p<0.05). Yüksek BAP konsantrasyonunun (0.15 mg/L) çalışılan tüm grupların yumru sayılarını azalttığı, ancak 3.5 g/L AC ile desteklendiğinde ölçülen tüm değerlerin artışını sağladığı bulunmuştur (p<0.05). ). Genel olarak, kallustan bitki kısımlarının en iyi şekilde büyümesi, en yüksek konsantrasyonda AC ve >1.5 mg/L BAP ile elde edilmiştir.
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Primary Language en
Subjects Agriculture
Journal Section RESEARCH ARTICLE
Authors

Orcid: 0000-0002-9903-5339
Author: Nigar SAEED (Primary Author)
Institution: KAHRAMANMARAŞ SÜTÇÜ İMAM ÜNİVERSİTESİ
Country: Turkey


Orcid: 0000-0003-4850-6927
Author: Sabahattin CÖMERTPAY
Institution: KAHRAMANMARAŞ SÜTÇÜ İMAM ÜNİVERSİTESİ
Country: Turkey


Supporting Institution Kahramanmaraş Sütçü İmam Üniversitesi Bilimsel Araştırma Projeleri Birimi
Project Number 2017/4-28D
Thanks This work was supported by Scientific Research Projects (BAP) Unit of Kahramanmaras Sutcu Imam University with the project number of 2017/4-28D.
Dates

Application Date : September 30, 2019
Acceptance Date : November 8, 2019
Publication Date : April 30, 2020

APA Saeed, N , Cömertpay, S . (2020). Effects of 6-benzylaminopurine and Activated Carbon on Indirect Organogenesis of Fritillaria Imperialis . Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 23 (2) , 297-303 . DOI: 10.18016/ksutarimdoga.vi.627056