Year 2020, Volume 23 , Issue 3, Pages 634 - 640 2020-06-30

Overexpression of Cytochrome P450 CYP6CM1 Gene in Bemisia tabaci (Gennadius) in the Cotton Fields of the Çukurova Region, Turkey
Çukurova Bölgesi Pamuk Alanlarında Bemisia tabaci (Gennadius)’de Aşırı Düzeyde Sitokrom P450 CYP6CM1 Gen Ekspresyonu

Selçuk ULUSOY [1]


In Cukurova region, neonicotinoid group insecticides are widely used in cotton cultivation areas for controlling B. tabaci; as a result of this use, there is a problem of resistance in these areas. In this study, cytochrome P450 CYP6CM1 relative expression levels, which are the indicators of imidacloprid resistance, were analyzed. Five different B. tabaci populations were collected from Adana city cotton cultivation areas in 2018 and the indicators were observed. On the other hand, GST gene relative expression profile, which is an efficient factor in metabolic resistance, cytochrome P450, carboxylesterase (AcHE) and Glutathion S transferase (GST) enzyme activities were determined. The highest cyp6cm1 and GST genes relative activities were determined in Meletmez population; the activity level was 18, 2 and 9 times higher than susceptible population. On the other hand, the highest and lowest cytochrome P450 enzyme activity was observed in Meletmez and Solaklı populations, respectively. According to the analyses, it can be concluded that there is extensive imidacloprid and neonicotinoid resistance of B. tabaci populations in Çukurova Region cotton cultivation areas. As a result, excessive gene expression and high-level enzyme activities indicate that there is a resistance in the studied area based on enforced monooxygenases activity. 

Çukurova bölgesi polikültür tarımın yapıldığı bir bölgedir. Bu bölgede pamuk ekim alanlarında B. tabaci mücadelesinde neonikotinoid grubu insektisitler yoğun bir şekilde kullanılmakta olup, direnç problemi ile karşılaşılmaktadır. Çalışmada 2018 yılı, Adana ili pamuk alanlarından toplanmış beş farklı B. tabaci popülasyonunda imidacloprid dayanıklılığı göstergesi olan sitokrom P450 CYP6CM1 geni relatif expresyon düzeyleri incelenmiştir. Ayrıca metabolik dayanıklılıkda etkili GST geni relatif expresyon profili, sitokrom P450, karboksil esteraz(AcHE) ve Glutathion S transferaz (GST) enzim etkinlikleri belirlenmiştir. En yüksek relatif aktivite Meletmez populasyonunda CYP6CM1 geni 18.2 kat ve Gst geni 9 kat daha fazla gen regülasyonu belirlenmiştir. Ayrıca en yüksek ve en düşük sitokrom P450 enzim aktivitesi meletmez ve solaklı popülasyonlarında gözlemlenmiştir. Yapılan analizlerde Çukurova bölgesi pamuk alanlarında B. tabaci popülasyonlarında yaygın imidacloprid ve neonikotinoid dayanıklılığı durumundan bahsedilebilir. Sonuç olarak, aşırı gen expresyonu ve yüksek düzeydeki enzim etkinlikleri, bölgede güçlendirilmiş monooksigenaz etkinliğine dayanan direncin varlığını ortaya koymaktadır.

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Primary Language en
Subjects Agriculture
Journal Section RESEARCH ARTICLE
Authors

Orcid: 0000-0002-2278-8471
Author: Selçuk ULUSOY (Primary Author)
Institution: Biyolojik Mücadele Araştırma Enstitüsü Müdürlüğü-ADANA
Country: Turkey


Dates

Application Date : August 16, 2019
Acceptance Date : January 21, 2020
Publication Date : June 30, 2020

APA Ulusoy, S . (2020). Overexpression of Cytochrome P450 CYP6CM1 Gene in Bemisia tabaci (Gennadius) in the Cotton Fields of the Çukurova Region, Turkey . Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 23 (3) , 634-640 . DOI: 10.18016/ksutarimdoga.vi.605716