Year 2020, Volume 23 , Issue 3, Pages 586 - 591 2020-06-30

Determination of Antioxidant and Oxidant Potentials of Pleurotus citrinopileatus Mushroom Cultivated on Various Substrates
Farklı Yetiştirme Ortamlarında Üretilen Pleurotus citrinopileatus Mantarının Antioksidan ve Oksidan Potansiyelinin Belirlenmesi

Ayşenur GÜRGEN [1] , Mustafa SEVİNDİK [2] , Sibel YILDIZ [3] , Hasan AKGÜL [4]


Many mushroom species have been used by people for different purposes, from past to present. Cultivated mushrooms may show different biological effects depending on the content of the substrate they grown on. The present study aimed to determine the total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) of Pleurotus citrinopileatus Singer mushroom cultivated on five different substrates. The cultivated mushrooms were extracted with ethanol in a Soxhlet device. TAS, TOS and OSI of extracts were determined with Rel Assay kits. The highest TAS (3.125±0.038 mmol/L), TOS (10.786±0.313 µmol/L) and OSI (0.345±0.014) values were determined in the mushrooms grown on 90% beech sawdust+10% bran. The lowest TAS (2.316±0.042), TOS (1.246±0.044) and OSI (0.054±0.001) values were obtained from the mushrooms grown on 100% poplar sawdust.

Geçmişten günümüze birçok mantar türü insanlar tarafından farklı amaçlar için kullanılmaktadır. Kültür mantarları, kullandıkları substratın içeriğine bağlı olarak farklı biyolojik etkiler gösterebilir. Bu çalışmada 5 farklı yetiştirme ortamından elde edilen Pleurotus citrinopileatus Singer mantarının toplam antioksidan seviyeleri (TAS), toplam oksidan seviyeleri (TOS) ve oksidatif stres indekslerinin (OSI) belirlenmesi amaçlanmıştır. Elde elde edilen mantarlar Soxhlet cihazında etanol ile ekstrakte edilmiştir.  Ekstraktların TAS, TOS ve OSI değerleri Rel Assay kitleri kullanılarak belirlenmiştir. En yüksek TAS (3.125±0.038), TOS (10.786±0.313) ve OSI (0.345±0.014) değerleri %90 kayın talaşı +%10 kepek ortamında yetiştirilen mantarlarda belirlenmiştir. En düşük TAS (2.316±0.042), TOS (1.246±0.044) ve OSI (0.054±0.001) değerleri ise %100 kavak talaşı ortamında yetiştirilen mantarlardan elde edilmiştir. 

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Primary Language en
Subjects Agriculture
Journal Section RESEARCH ARTICLE
Authors

Orcid: 0000-0002-2263-7323
Author: Ayşenur GÜRGEN (Primary Author)
Country: Turkey


Orcid: 0000-0001-7223-2220
Author: Mustafa SEVİNDİK

Orcid: 0000-0001-8448-4628
Author: Sibel YILDIZ

Orcid: 0000-0001-8514-9776
Author: Hasan AKGÜL

Dates

Application Date : September 30, 2019
Acceptance Date : January 9, 2020
Publication Date : June 30, 2020

APA Gürgen, A , Sevi̇ndi̇k, M , Yıldız, S , Akgül, H . (2020). Determination of Antioxidant and Oxidant Potentials of Pleurotus citrinopileatus Mushroom Cultivated on Various Substrates . Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 23 (3) , 586-591 . DOI: 10.18016/ksutarimdoga.vi.626803