Major Histocompatibility Gene Complex Polymorphism in Gerze Chicken and Some Pure Line Genotypes
Abstract
The aim of this study was
to molecularly investigate the major histocompatibility complex (MHC)
polymorphisms of the Gerze chicken population of 43 samples and the commercial
pure line population of 50 samples. Allelic diversity was calculated based on PCR
amplification of MCW0371 and LEI0258 microsatellite loci in MHC and separation
of DNA fragments by agarose gel electrophoresis. In the Gerze population,
number of different alleles, number of effective alleles, observed
heterozygosity and expected heterozygosity values were calculated as: 16,
10.82, 0.67, 0.91, whereas the values were determined as 19, 11.84, 0.60, 0.92
for the pure line population, respectively for LEI0258 locus. As for MCW0371
locus, the values were calculated as 3, 2.68, 0, 0.63 in the Gerze population
and 3, 1.96, 0, 0.49 in the Pure line population, respectively. Nei’s genetic
distance and genetic identity values between the populations were calculated as
0.419 and 0.658, respectively. The FST value among the populations
was found to be 0.068. The findings revealed that Gerze chicken should be
included in breeding programs as a unique genetic resource with its allelic
richness.
Keywords
Supporting Institution
Project Number
Thanks
References
- Abasht B, Dekkers J, Lamont S 2006. Review of quantitative trait loci identified in the chicken. Poultry Science 85(12): 2079-2096.
- Chazara O, Juul-Madsen H R, Chang C-S, Tixier-Boichard M, Bed’hom B 2011. Correlation in chicken between the marker LEI0258 alleles and major histocompatibility complex sequences. BMC proceedings 5(4): S29.
- Eimes J, Bollmer J, Whittingham L, Johnson J, Van Oosterhout C, Dunn P 2011. Rapid loss of MHC class II variation in a bottlenecked population is explained by drift and loss of copy number variation. Journal of Evolutionary Biology 24(9): 1847-1856.
- Emara M, Kim H, Zhu J, Lapierre R, Lakshmanan N, Lillehojt H 2002. Genetic diversity at the major histocompatibility complex (B) and microsatellite loci in three commercial broiler pure lines. Poultry Science 81(11): 1609-1617.
- Fadhil M, Mercan L 2016. Molecular characterization of mx gene polymorphism in gerze chicken breed and pure line chicken breed. Animal Research International 13(3): 2540.
- Fulton J E, Juul-Madsen H R, Ashwell C M, Mccarron A M, Arthur J A, O'sullivan N P, Taylor R L, Jr. 2006. Molecular genotype identification of the Gallus gallus major histocompatibility complex. Immunogenetics 58(5-6): 407-421.
- Gao C, Han L, Han J, Liu J, Jiang Q, Guo D, Qu L 2015. Establishment of six homozygous MHC-B haplotype populations associated with susceptibility to Marek’s disease in Chinese specific pathogen-free BWEL chickens. Infection, Genetics and Evolution 29:15-25.
- Mercan L, Bilgi F, Budak M 2019. Saf Gerze tavuğu ve Sinop ili köy tavuğu popülasyonlarının sekiz polimorfik mikrosatellit lokusu bakımından karşılaştırılması. Anadolu Tarım Bilimleri Dergisi 34(2): 164-171.
Details
Primary Language
Turkish
Subjects
Agricultural, Veterinary and Food Sciences
Journal Section
Research Article
Publication Date
June 30, 2020
Submission Date
November 20, 2019
Acceptance Date
January 16, 2020
Published in Issue
Year 1970 Volume: 23 Number: 3
