Abstract
Bu çalışmada Gerze tavuğu
popülasyonuna ait 43 örnek ile ticari saf hatlardan oluşturulan 50 örneklik Saf
hat popülasyonunun majör doku uyumluluğu gen kompleksi (MHC) bakımından
moleküler incelenmesi amaçlanmıştır. Allelik çeşitlilik, MHC içerisinde yer
alan MCW0371 ve LEI0258 mikrosatellit lokuslarının PCR yöntemi yardımıyla
çoğaltılarak elde edilen DNA fragmentlerinin agaroz jel elektroforezi ile
ayrımlanması ile hesaplanmıştır. Gerze popülasyonunda LEI0258 lokusunda elde
edilen allel sayısı, etkili allel sayısı, gözlenen heterozigotluk ve beklenen
heterozigotluk değerleri sırasıyla; 16, 10.82, 0.67, 0.91, Saf hat
popülasyonunda ise 19, 11.84, 0.60, 0.92 olarak bulunmuştur. MCW0371 lokusu
için sırasıyla; Gerze popülasyonunda 3, 2.68, 0, 0.63 ve Saf hat popülasyonunda
3, 1.96, 0, 0.49 olarak tespit edilmiştir. Popülasyonlar arasında Nei genetik
uzaklık ve genetik benzerlik değerleri sırasıyla 0.419 ve 0.658 olarak
hesaplanmıştır. Popülasyonlar arasında FST değeri 0.068 olarak
bulunmuştur. Elde edilen bulgular,
içerdiği allel deseni bakımından Gerze tavuğunun özgün bir genetik kaynak
olarak korunmasının ve ıslah programlarına dahil edilmesinin önemli olduğu
sonucuna varılmıştır.
Keywords
Supporting Institution
Ondokuz Mayıs Üniversitesi
Project Number
PYO.ZRT.1904.13.022
Thanks
Bu çalışmanın bir kısmı Ondokuz Mayıs Üniversitesi tarafından PYO.ZRT.1904.13.022 kodlu “Gerze tavuğunda majör doku uyumluluğu kompleksi (MHC) polimorfizminin mikrosatellit markörler ile karakterizasyonu” adlı Yüksek Lisans tez projesi kapsamında desteklenmiştir.
References
- Abasht B, Dekkers J, Lamont S 2006. Review of quantitative trait loci identified in the chicken. Poultry Science 85(12): 2079-2096.
- Chazara O, Juul-Madsen H R, Chang C-S, Tixier-Boichard M, Bed’hom B 2011. Correlation in chicken between the marker LEI0258 alleles and major histocompatibility complex sequences. BMC proceedings 5(4): S29.
- Eimes J, Bollmer J, Whittingham L, Johnson J, Van Oosterhout C, Dunn P 2011. Rapid loss of MHC class II variation in a bottlenecked population is explained by drift and loss of copy number variation. Journal of Evolutionary Biology 24(9): 1847-1856.
- Emara M, Kim H, Zhu J, Lapierre R, Lakshmanan N, Lillehojt H 2002. Genetic diversity at the major histocompatibility complex (B) and microsatellite loci in three commercial broiler pure lines. Poultry Science 81(11): 1609-1617.
- Fadhil M, Mercan L 2016. Molecular characterization of mx gene polymorphism in gerze chicken breed and pure line chicken breed. Animal Research International 13(3): 2540.
- Fulton J E, Juul-Madsen H R, Ashwell C M, Mccarron A M, Arthur J A, O'sullivan N P, Taylor R L, Jr. 2006. Molecular genotype identification of the Gallus gallus major histocompatibility complex. Immunogenetics 58(5-6): 407-421.
- Gao C, Han L, Han J, Liu J, Jiang Q, Guo D, Qu L 2015. Establishment of six homozygous MHC-B haplotype populations associated with susceptibility to Marek’s disease in Chinese specific pathogen-free BWEL chickens. Infection, Genetics and Evolution 29:15-25.
- Mercan L, Bilgi F, Budak M 2019. Saf Gerze tavuğu ve Sinop ili köy tavuğu popülasyonlarının sekiz polimorfik mikrosatellit lokusu bakımından karşılaştırılması. Anadolu Tarım Bilimleri Dergisi 34(2): 164-171.
- Mercan L, Okumuş A 2015. Genetic diversity of village chickens in Central Black Sea Region and commercial chickens in Turkey by using microsatellite markers. Turkish Journal of Veterinary & Animal Sciences 39(2): 134-140.
- Miller M M, Taylor Jr R L 2016. Brief review of the chicken major histocompatibility complex: the genes, their distribution on chromosome 16, and their contributions to disease resistance. Poultry Science 95(2): 375-392.
- Nei M 1977. F‐statistics and analysis of gene diversity in subdivided populations. Annals of human genetics 41(2): 225-233.
- Ngeno K, Van Der Waaij E, Megens H, Kahi A, Van Arendonk J, Crooijmans R 2015. Genetic diversity of different indigenous chicken ecotypes using highly polymorphic MHC-linked and non-MHC microsatellite markers. Animal Genetic Resources/Resources génétiques animales/Recursos genéticos animales 56:1-7.
- Rousset F 2008. genepop’007: a complete re‐implementation of the genepop software for Windows and Linux. Molecular ecology resources 8(1): 103-106.
- Sarıca M, Türkoğlu M 2009. Tavukçuluktaki Gelişmeler ve Türkiye Tavukçuluğu. (Tavukçuluk Bilimi Yetiştirme ve Hastalıklar, Bey Ofset Matbaacılık, Ankara, Türkiye: Ed. Türkoğlu, M Sarıca, M) 1-25.
- Smouse R P P, Peakall R 2012. GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research—an update. Bioinformatics 28(19): 2537-2539.
Abstract
The aim of this study was
to molecularly investigate the major histocompatibility complex (MHC)
polymorphisms of the Gerze chicken population of 43 samples and the commercial
pure line population of 50 samples. Allelic diversity was calculated based on PCR
amplification of MCW0371 and LEI0258 microsatellite loci in MHC and separation
of DNA fragments by agarose gel electrophoresis. In the Gerze population,
number of different alleles, number of effective alleles, observed
heterozygosity and expected heterozygosity values were calculated as: 16,
10.82, 0.67, 0.91, whereas the values were determined as 19, 11.84, 0.60, 0.92
for the pure line population, respectively for LEI0258 locus. As for MCW0371
locus, the values were calculated as 3, 2.68, 0, 0.63 in the Gerze population
and 3, 1.96, 0, 0.49 in the Pure line population, respectively. Nei’s genetic
distance and genetic identity values between the populations were calculated as
0.419 and 0.658, respectively. The FST value among the populations
was found to be 0.068. The findings revealed that Gerze chicken should be
included in breeding programs as a unique genetic resource with its allelic
richness.
Keywords
Project Number
PYO.ZRT.1904.13.022
References
- Abasht B, Dekkers J, Lamont S 2006. Review of quantitative trait loci identified in the chicken. Poultry Science 85(12): 2079-2096.
- Chazara O, Juul-Madsen H R, Chang C-S, Tixier-Boichard M, Bed’hom B 2011. Correlation in chicken between the marker LEI0258 alleles and major histocompatibility complex sequences. BMC proceedings 5(4): S29.
- Eimes J, Bollmer J, Whittingham L, Johnson J, Van Oosterhout C, Dunn P 2011. Rapid loss of MHC class II variation in a bottlenecked population is explained by drift and loss of copy number variation. Journal of Evolutionary Biology 24(9): 1847-1856.
- Emara M, Kim H, Zhu J, Lapierre R, Lakshmanan N, Lillehojt H 2002. Genetic diversity at the major histocompatibility complex (B) and microsatellite loci in three commercial broiler pure lines. Poultry Science 81(11): 1609-1617.
- Fadhil M, Mercan L 2016. Molecular characterization of mx gene polymorphism in gerze chicken breed and pure line chicken breed. Animal Research International 13(3): 2540.
- Fulton J E, Juul-Madsen H R, Ashwell C M, Mccarron A M, Arthur J A, O'sullivan N P, Taylor R L, Jr. 2006. Molecular genotype identification of the Gallus gallus major histocompatibility complex. Immunogenetics 58(5-6): 407-421.
- Gao C, Han L, Han J, Liu J, Jiang Q, Guo D, Qu L 2015. Establishment of six homozygous MHC-B haplotype populations associated with susceptibility to Marek’s disease in Chinese specific pathogen-free BWEL chickens. Infection, Genetics and Evolution 29:15-25.
- Mercan L, Bilgi F, Budak M 2019. Saf Gerze tavuğu ve Sinop ili köy tavuğu popülasyonlarının sekiz polimorfik mikrosatellit lokusu bakımından karşılaştırılması. Anadolu Tarım Bilimleri Dergisi 34(2): 164-171.
- Mercan L, Okumuş A 2015. Genetic diversity of village chickens in Central Black Sea Region and commercial chickens in Turkey by using microsatellite markers. Turkish Journal of Veterinary & Animal Sciences 39(2): 134-140.
- Miller M M, Taylor Jr R L 2016. Brief review of the chicken major histocompatibility complex: the genes, their distribution on chromosome 16, and their contributions to disease resistance. Poultry Science 95(2): 375-392.
- Nei M 1977. F‐statistics and analysis of gene diversity in subdivided populations. Annals of human genetics 41(2): 225-233.
- Ngeno K, Van Der Waaij E, Megens H, Kahi A, Van Arendonk J, Crooijmans R 2015. Genetic diversity of different indigenous chicken ecotypes using highly polymorphic MHC-linked and non-MHC microsatellite markers. Animal Genetic Resources/Resources génétiques animales/Recursos genéticos animales 56:1-7.
- Rousset F 2008. genepop’007: a complete re‐implementation of the genepop software for Windows and Linux. Molecular ecology resources 8(1): 103-106.
- Sarıca M, Türkoğlu M 2009. Tavukçuluktaki Gelişmeler ve Türkiye Tavukçuluğu. (Tavukçuluk Bilimi Yetiştirme ve Hastalıklar, Bey Ofset Matbaacılık, Ankara, Türkiye: Ed. Türkoğlu, M Sarıca, M) 1-25.
- Smouse R P P, Peakall R 2012. GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research—an update. Bioinformatics 28(19): 2537-2539.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Agricultural, Veterinary and Food Sciences |
| Journal Section | RESEARCH ARTICLE |
| Authors | |
| Project Number | PYO.ZRT.1904.13.022 |
| Publication Date | June 30, 2020 |
| Submission Date | November 20, 2019 |
| Acceptance Date | January 16, 2020 |
| Published in Issue | Year 2020Volume: 23 Issue: 3 |
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