Year 2020, Volume 23 , Issue 3, Pages 641 - 654 2020-06-30

Prevalence and Characterization of Plant Growth Promoting Mechanisms of Endophytic and Epiphytic Bacterial Species Isolated from Almond Trees Growing in Diyarbakır Province of Turkey
Diyarbakır İlinde Yetişen Badem Ağaçlarından Endofit ve Epifit Bakteri Türlerinin İzolasyonu ve Bitki Gelişimini Teşvik Eden Mekanizmalarının Karakterizasyonu

Zekiye Ceren Ceren AKTAN [1] , Soner SOYLU [2]


The aims of this study were to identify endophytic and epiphytic plant growth promoting bacteria (PGPB) from healthy almond trees and to characterize plant growth promoting mechanisms. By using MALDI-TOF analyses, 19 Gram-negative isolates, belonging to Serratia, Pseudomonas, Ochrobactrum and Enterobacter genus and 26 Gram-positive isolates, belonging to Bacillus and Arthrobacter genus were identified. Among the tested bacterial isolates, 46 isolates were positive for siderophore production, 45 isolates for ammonium production, 35 isolates solubilized phosphorus varying ratios, and all isolates produced IAA between 5.48-127.81 µg/ml concentrations. Isolates of Pseudomonas spp and Enterobacter cloacae were found highly effective for siderophore production, S. marcescens for solubilisation of phosphorus, E. cloacae for IAA production, Bacillus spp for ammonia production. Bacterial isolates tested showed significant differences in siderophore, ammonia and IAA production and phosphorus solubilisation efficiency. It was found that the efficacies of endophytic bacterial isolates were lower compared to the same species of epiphytic bacterial isolates. Overall results suggest that use of the most efficient PGPB isolates have an excellent potential to be used as biofertilizer for cultivation of economically important crops.

Bu çalışmanın amacı, sağlıklı badem ağaçlarından izole edilen bitki gelişimini teşvik eden endofit ve epifit bakteri (PGPB) türlerin tanılanması ve bitki gelişimini teşvik eden mekanizmalarının belirlemektir. MALDI-TOF tanılama analizleri sonucu, 19 izolat Gram-negatif Serratia, Pseudomonas, Ochrobactrum ve Enterobacter, 26 izolat ise Gram-pozitif Bacillus ve Arthrobacter cinsine dahil türler olarak teşhis edilmiştir. Test edilen izolatlardan, 46 izolat siderofor, 45 izolat amonyak üretiminde pozitif, 35 izolat ise fosforu değişen oranlarda çözebilme yeteneğinde olurken, bakterilerin tamamı 5.48-127.81 µg/ml konsantrasyon aralığında IAA üretmişlerdir. Pseudomonas spp and Enterobacter cloacae izolatları siderofor, Serratia marcescens izolatları fosforu çözme, Enterobacter cloacae izolatları IAA üretme, Bacillus spp ise amonyak üretme etkinliğinin yüksek düzeylerde olduğu görülmüştür. Aynı türe bağlı endofit bakteri izolatları epifit bakteri izolatlarına kıyasla bu mekanizmaları daha düşük düzeyde teşvik ettiği belirlenmiştir. Elde edilen sonuçlar yüksek düzeyde siderofor, amonyak, IAA üretimi ile fosfor çözme gibi bitki gelişimini teşvik edici özellikleri sahip PGPB izolatlarının ekonomik öneme sahip kültür bitkilerin yetiştiriciliğinde biyolojik gübre olarak kullanılmada potansiyeline sahip olduğunu göstermiştir.
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Primary Language tr
Subjects Agriculture
Journal Section RESEARCH ARTICLE
Authors

Orcid: 0000-0003-1031-8314
Author: Zekiye Ceren Ceren AKTAN
Institution: Tarım ve Orman Bakanlığı, Diyarbakır Zirai Mücadele Araştırma Enstitüsü Müdürlüğü
Country: Turkey


Orcid: 0000-0003-1002-8958
Author: Soner SOYLU (Primary Author)
Institution: Mustafa Kemal Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümü
Country: Turkey


Supporting Institution Hatay Mustafa Kemal Üniversitesi Bilimsel Araştırma Projeleri Komisyonu Başkanlığı
Project Number MKU BAP-16444
Thanks Bu çalışma Hatay Mustafa Kemal Üniversitesi Bilimsel Araştırma Projeleri Komisyonu Başkanlığı tarafından finansal olarak desteklenmiştir (Proje Numarası: MKU BAP-16444).
Dates

Application Date : December 16, 2019
Acceptance Date : February 24, 2020
Publication Date : June 30, 2020

APA Aktan, Z , Soylu, S . (2020). Diyarbakır İlinde Yetişen Badem Ağaçlarından Endofit ve Epifit Bakteri Türlerinin İzolasyonu ve Bitki Gelişimini Teşvik Eden Mekanizmalarının Karakterizasyonu. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 23 (3) , 641-654 . DOI: 10.18016/ksutarimdoga.vi.659802