Year 2020, Volume 23 , Issue 4, Pages 860 - 869 2020-08-31

Bazı Endemik ve Doğal Isatis L. Türlerine Ait Kök ve Gövde Ekstraktlarının Biyoaktivitesi ile Tohum Yağlarının Analizi
The Analysis of Seed Oil with Bioactivity of Root and Stem Extracts of some Endemic and Native Isatis L. Species

Nazan ÇÖMLEKCİOĞLU [1]


Bu çalışmada, HPLC analizleri sonucunda bitkilerde doğal glukozinolatlardan progoitrin, epiprogoitrin, glukonapin, glukoerusin, glukobrassisin olmak üzere beş farklı glukozinolat farklı miktarlarda belirlenmiştir. I. tinctoria ve I. tinctoria subsp. corymbosa’da köklerinde ana bileşenler progoitrin ve glukobrassisin iken, diğer üç türde glukonapin ve glukobrassisin olarak bulunmuştur. Gövdeye oranla kökte daha yüksek oranda glukozinolat, fenolik, flavonoid madde ve protein miktarı tespit edilmiştir. Ayrıca kök ekstraktlarının antioksidan aktivitesinin gövdeye oranla daha yüksek olduğu bulunmuştur. Bitkilerin kök ve gövde ekstraktlarının her ikisi de gram pozitif bakterilerin büyümesini inhibe ederken, gram negatif bakterilerin büyümesini yalnızca kök ekstraktlarının inhibe ettiği görülmüştür. I. aucherii, I. buschiana, I. candolleana, I. tinctoria ve I. tinctoria subsp. corymbosa tohumlarının yağ içerikleri sırasıyla % 30.41, 37.55, 38.43, 28.79 ve 36.45 olarak elde edilmiştir. Çalışılan tüm türlerde yağın önemli bir kısmının doymamış yağ asitlerinden (oleik, linoleik, alfa-linolenik, cis-11 eikosenoik ve erusik asit) oluştuğu belirlenmiştir.

In this study, five different glucosinolates including progoitrin, epiprogoitrin, gluconapine, glucoerucine, glucobrassicin were determined in different plant species by using HPLC. The main constituents of I. tinctoria and I. tinctoria subsp. corymbosa roots were progoitrin and glucobrassicin, while the other three species contained gluconapine and glucobrassicin. A higher amount of glucosinolate, phenolic, flavonoid substance and protein were detected in the plant roots than in the stems. The antioxidant activity of root extracts was found higher than the stem. The root and stem extracts of plants showed significant inhibition on gram-positive bacteria, but the only root extracts showed inhibition on gram negative bacteria. The oil content of I. aucherii, I. buschiana, I. candolleana, I. tinctoria and I. tinctoria subsp. Corymbosa seeds were 30.41%, 37.55%, 38.43%, 28.79% and 36.45%, respectively. Major fatty acid component of the all plant species studied were the unsaturated fats like oleic, linoleic, alpha-linolenic, cis-11 eicosenoic and erucic acid. 

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Primary Language tr
Subjects Biology
Journal Section RESEARCH ARTICLE
Authors

Orcid: 0000-0001-7729-5271
Author: Nazan ÇÖMLEKCİOĞLU (Primary Author)
Institution: Üniversite
Country: Turkey


Supporting Institution KSÜ BAP
Project Number Proje No:2012/3-23M
Thanks Bu çalışma KSÜ tarafından desteklenmiştir (Proje No:2012/3-23M).
Dates

Application Date : December 9, 2019
Acceptance Date : March 13, 2020
Publication Date : August 31, 2020

APA Çömlekci̇oğlu, N . (2020). Bazı Endemik ve Doğal Isatis L. Türlerine Ait Kök ve Gövde Ekstraktlarının Biyoaktivitesi ile Tohum Yağlarının Analizi . Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 23 (4) , 860-869 . DOI: 10.18016/ksutarimdoga.vi.657322