Year 2020, Volume 23 , Issue 4, Pages 876 - 884 2020-08-31

Alleviation of Everzol Red LFB Toxicity in Duckweed (Lemna minor L.) by Exogenous Salicylic Acid
Su Mercimeğinde (Lemna minor L.) Everzol Red LFB Toksisitesinin Eksojen Salisilik Asit ile Azaltılması

Gülçin BEKER AKBULUT [1] , Duygu ÖZHAN TURHAN [2] , Emel YİĞİT [3]


Salicylic acid (SA) has an important function in the formation of induced systemic resistance. The aim of this study was to determine effects of exogenous application of 0.5 mM SA on the stress response in duckweed (Lemna minor L.) exposed to the reactive dyestuff Everzol Red LFB (ER LFB). Phytotoxic responses induced by exposure to both ER LFB (75 ppm, 150 ppm and 300 ppm) and 0.5 mM SA+ ER LFB (75 ppm, 150 ppm and 300 ppm) applications were comparatively examined at 1st, 4th, and 7th days. The lowest chlorophyll a (Chl-a), chlorophyll b (Chl-b), and total chlorophyll (total Chl) contents were found in the 300 ppm ER LFB groups. The carotenoid (Car) content was decreased compared to control groups. The highest total glutathione (GSH), glutathione S-transferase (GST) and peroxidase (POD) activities were found after 0.5 mM SA + 300 ppm ER LFB groups at 7th day. Glutathione reductase (GR) activity was reduced at 7th day. The level of lipid peroxidation, measured as malondialdehyde (MDA) content was increased generally both ER LFB and SA+ER LFB groups compared to control groups. Results suggest that SA plays a positive role in L. minor against ER LFB.
Salisilik asit (SA) sistemik direncin oluşmasında önemli bir role sahiptir. Bu çalışmanın amacı eksojen uygulanan 0.5 mM SA’nın reaktif boya maddesi Everzol Red LFB (ER LFB)’ye maruz kalan su mercimeğindeki (Lemna minor L.) stress tepkisi üzerindeki etkilerini belirlemektir. Hem ER LFB (75 ppm, 150 ppm ve 300 ppm) hem de 0.5 mM SA + ER LFB (75 ppm, 150 ppm ve 300 ppm) uygulamalarına maruz kalmanın neden olduğu fitotoksik tepkiler 1., 4. ve 7. günlerde karşılaştırmalı olarak incelenmiştir. En düşük klorofil a (Kl-a), klorofil b (Kl-b) ve toplam klorofil (toplam Kl) içeriği 300 ppm ER LFB grubunda bulunmuştur. Karotenoid (Kar) içeriği kontrol gruplarına göre azalmıştır. En yüksek toplam glutatyon (GSH), glutatyon S-transferaz (GST) ve peroksidaz (POD) aktiviteleri 7. günde 0.5 mM SA + 300 ppm ER LFB gruplarında bulunmuştur. Glutatyon redüktaz (GR) aktivitesi 7. günde azalmıştır. Malondialdehid (MDA) içeriği olarak ölçülen lipit peroksidasyon seviyesi, kontrol gruplarına kıyasla genellikle hem ER LFB hem de SA + ER LFB gruplarında artmıştır. Sonuçlar SA'nın L. minor’de ER LFB'ye karşı pozitif bir rol oynadığını göstermektedir.
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Primary Language en
Subjects Biology
Journal Section RESEARCH ARTICLE
Authors

Orcid: 0000-0002-4964-6780
Author: Gülçin BEKER AKBULUT (Primary Author)
Institution: MALATYA TURGUT ÖZAL ÜNİVERSİTESİ
Country: Turkey


Orcid: 0000-0002-7111-4289
Author: Duygu ÖZHAN TURHAN
Institution: MALATYA İNÖNÜ ÜNİVERSİTESİ
Country: Turkey


Orcid: 0000-0001-6333-8437
Author: Emel YİĞİT
Institution: MALATYA İNÖNÜ ÜNİVERSİTESİ
Country: Turkey


Dates

Application Date : February 3, 2020
Acceptance Date : April 2, 2020
Publication Date : August 31, 2020

APA Beker Akbulut, G , Özhan Turhan, D , Yi̇ği̇t, E . (2020). Alleviation of Everzol Red LFB Toxicity in Duckweed (Lemna minor L.) by Exogenous Salicylic Acid . Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 23 (4) , 876-884 . DOI: 10.18016/ksutarimdoga.vi.683962