Research Article
Evaluation of F5 Individuals Obtained from B28×Kunduru-1149 Reciprocal Cross Population by Functional Markers
Abstract
In the study, B28 and Kunduru-1149 durum wheat genotypes were crossed as reciprocal in 2012-2013 cropping season. 13 (B28/Kunduru-1149 and Kunduru-1149/B28) reciprocal crosses were obtained and were used as materials at F5 stage. The cross combinations and the parents were screened with 10 DNA markers to determine alleles of gluten strength (Bx7OE), Yellow rust (Sun104, Xgwm18, Xwgp115 and Xgwm47), stem rust (Sun209 and Sun479), high protein ratio (UHW89), powdery mildew (Xgwm66) and leaf rust (Xgwm130). In the study, the average polymorphism information content (PIC) was calculated as 0.98 and the lowest PIC value was obtained from Xwgp115 marker with 0.96, while the rest of the markers had 0.99 PIC values. Stem rust resistance allele Sr49 was detected in B28/Kunduru-1149_F5_4 (Sun479) and B28/Kunduru-1149_F5_1 (Sun209) genotypes. One of the yellow rust resistance alleles Yr15 (Xgwm18) was detected in B28/Kunduru-1149_F5_2 and B28/Kunduru-1149_F5_3 genotypes, while Yr51 (Sun104) was identified in B28/Kunduru-1149_F5_3, B28/Kunduru-1149_F5_6, B28/Kunduru-1149_F5_7, Kunduru-1149/B28_F5_2 and Kunduru-1149/B28_F5_6 genotypes. A dendrogram was created to determine kinship of the individuals with the parents. The highest genetic similarity was observed between B28 / Kunduru-1149_F5_6 and Kunduru-1149 / B28_F5_2 genotypes with 0.714, while the most diverse ones were Kunduru-1149 and B28/Kunduru_F5_7 with 0.10.
Supporting Institution
The Scientific Research Projects Council of Kahramanmaraş Sütçü İmam University
Project Number
2017/7-175 YLS
Thanks
This study is produced from the master thesis of Gülan AYDEMİR. The other authors were responsible for making crosses and bringing generations from F1 to F5. The abstract is also published in International Biological, Agricultural & Life Science Congress November 7-8, 2019 Lviv, Ukraine. Authors also would like to thank The Scientific Research Projects Council of Kahramanmaraş Sütçü İmam University (Project Number 2017/7-175 YLS).
References
- Anonymous 2016. Turkey’s Wheat Atlas, © WWF-Turkey (Wildlife Protection Foundation). http://d2hawiim0tjbd8.cloudfront.net/downloads/turkiye_nin_buday_atlas_web.pdf Date of Access: 19.05.2019
- Bansal UK, Muhammad S, Forrest KL, Hayden MJ, Bariana HS 2015. Mapping of a New Stem Rust Resistance Gene Sr49 in Chromosome 5B of Wheat. Theor Appl Genet 128 (10): 2113-2119.
- Butow BJ, Ma W, Gale KR, Cornish GB, Rampling L, Larroqueand O, Bekes F 2003. Molecular Discrimination of Bx7OE Alleles Demonstrates That a Highly Expressed high- Molecular-Weight Glutenin Allele Has a Major Impact on Wheat Flourdough Strength. Theor Appl Genet 107(8), 1524‒1532.
- Cho SW, Roy SK, Chun JB, Cho K, Park CS 2017. Overexpression of The Bx7 High Molecular Weight Glutenin Subunit on The Glu-B1 Locus in a Korean Wheat Landrace. Plant Biotechnol. Rep 11: 97‒105.
- Cowger C, Miranda L, Griffey C, Hall M, Murphrand JP, Maxwells J 2012. Wheat Powdery Mildew, Disease Resistance in Wheat. Sharma, I. (ed.) CABI Publications London, UK.
- Dice LR 1945. Measures of The Amount of Ecologic Association Between Species. Ecology 26: 297‒302.
- Distelfeld A, Uauy C, Fahimaand T, Dubcovsky J 2006. Physical Map of The Wheathigh-Grain Protein Content Gene Gpc-B1 and Development of a High- Through Put Molecular Marker. New Phytologist 169: 753‒763.
- Dumlupinar Z, Brown R, Campbell R, Jellen EN. Anderson J, Bonman M, Carson M, Chao S, Obert D, Jackson E 2016. The Art of Attrition: Development of Robust Oat Microsatellites. Plant Breed 135: 323‒334.
- Gungor H 2019. Allelic Variations and Agronomic Comparisons Of Durum Wheat Cultivars Under East-Mediterranean Conditions. Intl. J. Agric. Biol 21: 891‒898.
- Liang D, Tang J, Pena RJ, Singh R, He X, Shen X, He Z 2010. Characterization of CIMMYT Bread Wheats for High-And Low-Molecular Weight Glutenin Subunits and Other Quality-Related Genes with SDS-PAGE, RP-HPLC and Molecular Markers. Euphytica 172: 235-250.
- Maccaferri M, Sanguineti MC, Donini P, Tuberosa R 2003. Microsatellite Analysis Reveals a Progressive Widening of The Genetic Basis in The Elite Durum Wheat Germplasm. Theor Appl Genet 107: 783-797.
- Moragues M, Moralejo M, Sorrells ME, Royo C 2007. Dispersal of Durum Wheat [Triticum Turgidum L. Ssp. Turgidum Convar. Durum (Desf.) MacKey] Landraces Across The Mediterranean Basin Assessed by AFLPs and Microsatellites. Genet Resour Crop Evol 54: 1133-1144.
- Randhawa M, Bansal U, Valarik M, Klocova B, Dolezeland J, Bariana H 2014. Molecular Mapping Of Stripe Rust Resistance Gene Yr51 in Chromosome 4 AL of Wheat. Theor Appl Genet 127: 317-324.
- Ren J, Sun D, Chen L, You FM, Wang J, Peng Y, Nevo E, Sun D, Luo MJ, Peng J 2013. Genetic Diversity Revealed by Single Nucleotide Polymorphism Markers in a Worldwide Germplasm Collection of Durum Wheat. Intl J Mol Sci 14: 7061-7088.
- Roder MS, KorzunV, Wendehake K, Plaschke J, Tixier M, Leroyand P, Ganal MW 1998. A Microsatellite Map Of Wheat. Genetics, 149: 2007-2023.
- Rohlf FJ 2005. NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System Version 2.2. Setauket, Exeter Publishing, NY, USA.
- Sevinç D 2010. Sarı Pas Hastalığına Dayanıklı ve Duyarlı Buğday (Triticum aestivum L.) Çeşitlerinin Moleküler Markörler ile İncelenmesi. İstanbul Üniversitesi Fen Bilimleri Enstitüsü Biyoloji Anabilim Dalı Botanik Programı, Yüksek Lisans Tezi, 102s.
- TUIK 2018. Turkish Statistical Institute. www.tuik.gov.tr. Date of Access 05.06.2019.
- Weir BS 1996. Genetic Data Analysis II, 2nd ed. Sinauer Associates Inc. ISBN-13: 978-0878939022 Sunderland, Massachusetts, USA. 437s.
- Yan GP, Chen XM, Line RF, Wellings CR 2003. Resistance Gene-Analog Polymorphism Markers Co-Segregating with The YR5 Gene for Resistance to Wheat Stripe Rust. Theor Appl Genet 106: 636‒643.
B28×Kunduru-1149 Resiproklu Melez Popülasyonundan Elde Edilen F5 Bireylerinin Fonksiyonel Markörlerle Değerlendirilmesi
Abstract
Bu çalışmada, B28 ve Kunduru-1149 makarnalık buğday genotipleri 2012-2013 ürün sezonunda resiproklu olarak melezlenmiş ve elde edilen 13 B28/Kunduru-1149-Kunduru-1149/B28 F5 kademesindeki hat materyal olarak kullanılmıştır. Melez kombinasyonları ve ebeveynler gluten dayanıklılığı (Bx7OE), sarı pas (Sun104, Xgwm18, Xwgp115 ve Xgwm47), kara pas (Sun209 ve Sun479), yüksek protein oranı (UHW89), külleme (Xgwm66) ve kahverengi pas (Xgwm130) allelleri bakımından 10 DNA markörü ile taranmıştır. Çalışmada, ortalama polimorfizmi bilgi içeriği (PIC) 0.98 olarak hesaplanmış ve en düşük PIC değeri 0.96 ile Xwgp115 marköründen elde edilirken, diğer markörler 0.99 PIC değerine sahip olmuştur. B28/Kunduru-1149_F5_4 (Sun479) ve B28/Kunduru-1149_F5_1 (Sun209) genotiplerinde kara pasa dayanıklılık alleli Sr49 tespit edilmiştir. Sarı pas dayanıklılık allellerinden Yr15 (Xgwm18) B28/Kunduru-1149_F5_2 ve B28/Kunduru-1149_F5_3 genotiplerinde bulunurken, Yr51 (Sun104) geni B28/Kunduru-1149_F5_3, B28/Kunduru-1149_F5_6, B28/Kunduru-1149_F5_7, Kunduru-1149/B28_F5_2 ve Kunduru-1149/B28_F5_6 genotiplerinde tanımlanmıştır. Ebeveynler ile genotiplerin akrabalıklarını belirlemek için bir dendrogram oluşturulmuştur. En yüksek genetik benzerlik oranı B28 / Kunduru-1149_F5_6 ve Kunduru-1149 / B28_F5_2 genotipleri arasında 0.714 olarak tespit edilirken, birbirlerine en uzak genotipler 0.10 benzerlik oranı ile Kunduru-1149 ve B28/Kunduru_F5_7 olmuştur.
Project Number
2017/7-175 YLS
References
- Anonymous 2016. Turkey’s Wheat Atlas, © WWF-Turkey (Wildlife Protection Foundation). http://d2hawiim0tjbd8.cloudfront.net/downloads/turkiye_nin_buday_atlas_web.pdf Date of Access: 19.05.2019
- Bansal UK, Muhammad S, Forrest KL, Hayden MJ, Bariana HS 2015. Mapping of a New Stem Rust Resistance Gene Sr49 in Chromosome 5B of Wheat. Theor Appl Genet 128 (10): 2113-2119.
- Butow BJ, Ma W, Gale KR, Cornish GB, Rampling L, Larroqueand O, Bekes F 2003. Molecular Discrimination of Bx7OE Alleles Demonstrates That a Highly Expressed high- Molecular-Weight Glutenin Allele Has a Major Impact on Wheat Flourdough Strength. Theor Appl Genet 107(8), 1524‒1532.
- Cho SW, Roy SK, Chun JB, Cho K, Park CS 2017. Overexpression of The Bx7 High Molecular Weight Glutenin Subunit on The Glu-B1 Locus in a Korean Wheat Landrace. Plant Biotechnol. Rep 11: 97‒105.
- Cowger C, Miranda L, Griffey C, Hall M, Murphrand JP, Maxwells J 2012. Wheat Powdery Mildew, Disease Resistance in Wheat. Sharma, I. (ed.) CABI Publications London, UK.
- Dice LR 1945. Measures of The Amount of Ecologic Association Between Species. Ecology 26: 297‒302.
- Distelfeld A, Uauy C, Fahimaand T, Dubcovsky J 2006. Physical Map of The Wheathigh-Grain Protein Content Gene Gpc-B1 and Development of a High- Through Put Molecular Marker. New Phytologist 169: 753‒763.
- Dumlupinar Z, Brown R, Campbell R, Jellen EN. Anderson J, Bonman M, Carson M, Chao S, Obert D, Jackson E 2016. The Art of Attrition: Development of Robust Oat Microsatellites. Plant Breed 135: 323‒334.
- Gungor H 2019. Allelic Variations and Agronomic Comparisons Of Durum Wheat Cultivars Under East-Mediterranean Conditions. Intl. J. Agric. Biol 21: 891‒898.
- Liang D, Tang J, Pena RJ, Singh R, He X, Shen X, He Z 2010. Characterization of CIMMYT Bread Wheats for High-And Low-Molecular Weight Glutenin Subunits and Other Quality-Related Genes with SDS-PAGE, RP-HPLC and Molecular Markers. Euphytica 172: 235-250.
- Maccaferri M, Sanguineti MC, Donini P, Tuberosa R 2003. Microsatellite Analysis Reveals a Progressive Widening of The Genetic Basis in The Elite Durum Wheat Germplasm. Theor Appl Genet 107: 783-797.
- Moragues M, Moralejo M, Sorrells ME, Royo C 2007. Dispersal of Durum Wheat [Triticum Turgidum L. Ssp. Turgidum Convar. Durum (Desf.) MacKey] Landraces Across The Mediterranean Basin Assessed by AFLPs and Microsatellites. Genet Resour Crop Evol 54: 1133-1144.
- Randhawa M, Bansal U, Valarik M, Klocova B, Dolezeland J, Bariana H 2014. Molecular Mapping Of Stripe Rust Resistance Gene Yr51 in Chromosome 4 AL of Wheat. Theor Appl Genet 127: 317-324.
- Ren J, Sun D, Chen L, You FM, Wang J, Peng Y, Nevo E, Sun D, Luo MJ, Peng J 2013. Genetic Diversity Revealed by Single Nucleotide Polymorphism Markers in a Worldwide Germplasm Collection of Durum Wheat. Intl J Mol Sci 14: 7061-7088.
- Roder MS, KorzunV, Wendehake K, Plaschke J, Tixier M, Leroyand P, Ganal MW 1998. A Microsatellite Map Of Wheat. Genetics, 149: 2007-2023.
- Rohlf FJ 2005. NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System Version 2.2. Setauket, Exeter Publishing, NY, USA.
- Sevinç D 2010. Sarı Pas Hastalığına Dayanıklı ve Duyarlı Buğday (Triticum aestivum L.) Çeşitlerinin Moleküler Markörler ile İncelenmesi. İstanbul Üniversitesi Fen Bilimleri Enstitüsü Biyoloji Anabilim Dalı Botanik Programı, Yüksek Lisans Tezi, 102s.
- TUIK 2018. Turkish Statistical Institute. www.tuik.gov.tr. Date of Access 05.06.2019.
- Weir BS 1996. Genetic Data Analysis II, 2nd ed. Sinauer Associates Inc. ISBN-13: 978-0878939022 Sunderland, Massachusetts, USA. 437s.
- Yan GP, Chen XM, Line RF, Wellings CR 2003. Resistance Gene-Analog Polymorphism Markers Co-Segregating with The YR5 Gene for Resistance to Wheat Stripe Rust. Theor Appl Genet 106: 636‒643.
There are 20 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Agricultural, Veterinary and Food Sciences |
| Journal Section | RESEARCH ARTICLE |
| Authors | |
| Project Number | 2017/7-175 YLS |
| Publication Date | August 31, 2020 |
| Submission Date | February 11, 2020 |
| Acceptance Date | April 24, 2020 |
| Published in Issue | Year 2020Volume: 23 Issue: 4 |
Cite
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