Year 2020, Volume 23 , Issue 4, Pages 1005 - 1011 2020-08-31

B28×Kunduru-1149 Resiproklu Melez Popülasyonundan Elde Edilen F5 Bireylerinin Fonksiyonel Markörlerle Değerlendirilmesi
Evaluation of F5 Individuals Obtained from B28×Kunduru-1149 Reciprocal Cross Population by Functional Markers

Gülan AYDEMİR [1] , Ziya DUMLUPINAR [2] , İlker YÜCE [3] , Tuğba NARLI BAŞKONUŞ [4] , Selçuk SUNULU [5] , Hüseyin GÜNGÖR [6]


Bu çalışmada, B28 ve Kunduru-1149 makarnalık buğday genotipleri 2012-2013 ürün sezonunda resiproklu olarak melezlenmiş ve elde edilen 13 B28/Kunduru-1149-Kunduru-1149/B28 F5 kademesindeki hat materyal olarak kullanılmıştır. Melez kombinasyonları ve ebeveynler gluten dayanıklılığı (Bx7OE), sarı pas (Sun104, Xgwm18, Xwgp115 ve Xgwm47), kara pas (Sun209 ve Sun479), yüksek protein oranı (UHW89), külleme (Xgwm66) ve kahverengi pas (Xgwm130) allelleri bakımından 10 DNA markörü ile taranmıştır. Çalışmada, ortalama polimorfizmi bilgi içeriği (PIC) 0.98 olarak hesaplanmış ve en düşük PIC değeri 0.96 ile Xwgp115 marköründen elde edilirken, diğer markörler 0.99 PIC değerine sahip olmuştur. B28/Kunduru-1149_F5_4 (Sun479) ve B28/Kunduru-1149_F5_1 (Sun209) genotiplerinde kara pasa dayanıklılık alleli Sr49 tespit edilmiştir. Sarı pas dayanıklılık allellerinden Yr15 (Xgwm18) B28/Kunduru-1149_F5_2 ve B28/Kunduru-1149_F5_3 genotiplerinde bulunurken, Yr51 (Sun104) geni B28/Kunduru-1149_F5_3, B28/Kunduru-1149_F5_6, B28/Kunduru-1149_F5_7, Kunduru-1149/B28_F5_2 ve Kunduru-1149/B28_F5_6 genotiplerinde tanımlanmıştır. Ebeveynler ile genotiplerin akrabalıklarını belirlemek için bir dendrogram oluşturulmuştur. En yüksek genetik benzerlik oranı B28 / Kunduru-1149_F5_6 ve Kunduru-1149 / B28_F5_2 genotipleri arasında 0.714 olarak tespit edilirken, birbirlerine en uzak genotipler 0.10 benzerlik oranı ile Kunduru-1149 ve B28/Kunduru_F5_7 olmuştur.
In the study, B28 and Kunduru-1149 durum wheat genotypes were crossed as reciprocal in 2012-2013 cropping season. 13 (B28/Kunduru-1149 and Kunduru-1149/B28) reciprocal crosses were obtained and were used as materials at F5 stage. The cross combinations and the parents were screened with 10 DNA markers to determine alleles of gluten strength (Bx7OE), Yellow rust (Sun104, Xgwm18, Xwgp115 and Xgwm47), stem rust (Sun209 and Sun479), high protein ratio (UHW89), powdery mildew (Xgwm66) and leaf rust (Xgwm130). In the study, the average polymorphism information content (PIC) was calculated as 0.98 and the lowest PIC value was obtained from Xwgp115 marker with 0.96, while the rest of the markers had 0.99 PIC values. Stem rust resistance allele Sr49 was detected in B28/Kunduru-1149_F5_4 (Sun479) and B28/Kunduru-1149_F5_1 (Sun209) genotypes. One of the yellow rust resistance alleles Yr15 (Xgwm18) was detected in B28/Kunduru-1149_F5_2 and B28/Kunduru-1149_F5_3 genotypes, while Yr51 (Sun104) was identified in B28/Kunduru-1149_F5_3, B28/Kunduru-1149_F5_6, B28/Kunduru-1149_F5_7, Kunduru-1149/B28_F5_2 and Kunduru-1149/B28_F5_6 genotypes. A dendrogram was created to determine kinship of the individuals with the parents. The highest genetic similarity was observed between B28 / Kunduru-1149_F5_6 and Kunduru-1149 / B28_F5_2 genotypes with 0.714, while the most diverse ones were Kunduru-1149 and B28/Kunduru_F5_7 with 0.10.
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Primary Language en
Subjects Agriculture
Journal Section RESEARCH ARTICLE
Authors

Orcid: 0000-0001-6054-8893
Author: Gülan AYDEMİR
Institution: KAHRAMANMARAŞ SÜTÇÜ İMAM ÜNİVERSİTESİ
Country: Turkey


Orcid: 0000-0001-6054-8893
Author: Ziya DUMLUPINAR (Primary Author)
Institution: Kahramanmaraş Sütçü İmam Üniversitesi
Country: Turkey


Orcid: 0000-0002-9761-3561
Author: İlker YÜCE
Institution: Kahramanmaraş Sütçü İmam Üniversitesi
Country: Turkey


Orcid: 0000-0002-0744-6086
Author: Tuğba NARLI BAŞKONUŞ
Institution: Kahramanmaraş Sütçü İmam Üniversitesi
Country: Turkey


Orcid: 0000-0002-1806-2547
Author: Selçuk SUNULU
Institution: Kahramanmaraş Sütçü İmam Üniversitesi
Country: Turkey


Orcid: 0000-0001-6708-6337
Author: Hüseyin GÜNGÖR
Institution: Düzce Üniversitesi
Country: Turkey


Supporting Institution The Scientific Research Projects Council of Kahramanmaraş Sütçü İmam University
Project Number 2017/7-175 YLS
Thanks This study is produced from the master thesis of Gülan AYDEMİR. The other authors were responsible for making crosses and bringing generations from F1 to F5. The abstract is also published in International Biological, Agricultural & Life Science Congress November 7-8, 2019 Lviv, Ukraine. Authors also would like to thank The Scientific Research Projects Council of Kahramanmaraş Sütçü İmam University (Project Number 2017/7-175 YLS).
Dates

Application Date : February 11, 2020
Acceptance Date : April 24, 2020
Publication Date : August 31, 2020

APA Aydemi̇r, G , Dumlupınar, Z , Yüce, İ , Narlı, T , Sunulu, S , Güngör, H . (2020). Evaluation of F5 Individuals Obtained from B28×Kunduru-1149 Reciprocal Cross Population by Functional Markers . Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 23 (4) , 1005-1011 . DOI: 10.18016/ksutarimdoga.vi.687935