Year 2020, Volume 23 , Issue 5, Pages 1314 - 1321 2020-10-31

Süne, Eurygaster integriceps’in Put. (Heteroptera: Scutelleridae) Diyapoz Öncesi ve Diyapoz Sonrası Erginlerinden Hazırlanan Fosfolipit Altsınıflarının Yağ Asidi Bileşiminindeki Değişiklikler
The Changes in Composition of Phospholipid Subclasses Fatty Acids Prepared from Prediapausing and Postdiapausing Adults of Sunn Pest, Eurygaster integriceps Put. (Heteroptera: Scutelleridae)

Mehmet BAŞHAN [1] , Mehmet Cemal TALAY [2] , Vedat KARACA [3]


Bu çalışmada, süne, Eurygaster integriceps’in diyapoz öncesi ergin ve diyapoz sonrası erginlerinin fosfatidilkolin (PC), fosfatidiletanolamin (PE), fosfatidilinositol (PI) ve fosfatidilserin (PS) gibi fosfolipit (PL) altsınıflarının yağ asidi kompozisyonundaki değişiklikler araştırılmıştır. Fosfolipit alt sınıflarının ayrılmasında İnce Tabaka Kromatoğrafisi tekniği, yağ asitlerinin analizinde ise Gaz kromatoğrafi cihazı kullanılmıştır. Eurygaster integriceps’in PL altsınıflarında gözlenen dominant yağ asitleri, doymuş yağ asitlerinden (SFA) palmitik asit (16:0), tekli doymamış yağ asitlerinden (MUFA) oleik asit (18:1n-9) ve çoklu doymamış yağ asitlerinden (PUFA) linoleik asit (18:2n-6) idi. Diyapoz öncesi ve diyapoz sonrası erginlerin PC, PE ve PS fraksiyonlarının yağ asidi içeriğindeki değişikliklerin benzer olduğu bulunmuştur. Diyapoz sonrası erginlerin bu fraksiyonlarında 16:0, 18:0 ve ∑SFA yüzdeleri diyapoz öncesi erginlere oranla daha düşük; ancak16:1n-7, ∑MUFA, 18:2n-6 ve ∑PUFA ise daha yüksek olarak bulunmuştur. Sünenin diyapoz sonrası bireylerin PE ve PC fraksiyonlarındaki 18:1n-9 düzeyi, diyapoz öncesi bireylerden önemli derecede daha yüksek olarak tespit edilmiştir. Diyapoz sonrası erginlerin tüm PL altsınıflarındaki doymamış yağ asitlerinin doymuş yağ asitlere oranı (UFA/SFA), diyapoz öncesi erginlerden daha yüksek bulunmuştur.

In this study, the changes in fatty acid composition of phospholipid (PL) subclasses such as phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), and phosphatidylserine (PS) of pre-diapausing and post-diapausing adults of sunn pest Eurygaster integriceps were investigated. Thin Layer Chromatography technique was used to separate phospholipid subclasses and Gas Chromatography equipment was used for the analysis of fatty acids. The dominant fatty acids observed in PL subclasses of E. integriceps were palmitic acid (16:0) among saturated fatty acids (SFAs), oleic acid (18:1n-9) among monounsaturated fatty acids (MUFAs) and linoleic acid (18:2n-6) among polyunsaturated fatty acids (PUFAs). It was found that the changes in fatty acid compositions from the PC, PE and PS fractions of prediapausing and postdiapausing adults were similar. In these fractions of postdiapausing adults, the percentages of 16: 0, stearic acid (18:0) and ∑SFA were lower than in prediapausing adults; however, palmitoleic acid (16: 1n-7, ∑MUFA, 18: 2n-6 and ∑PUFA were higher. The level of oleic acid in PE and PC fractions of postdiapausing individuals of the E. integriceps was determined to be significantly higher than prediapausing individuals. The ratio of unsaturated fatty acids to saturated fatty acids (UFA / SFA) in all PL subclasses of postdiapausing adults was found to be higher than those of prediapausing adults.

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Primary Language tr
Subjects Biology
Journal Section RESEARCH ARTICLE
Authors

Orcid: 0000-0002-1228-9548
Author: Mehmet BAŞHAN (Primary Author)
Institution: Dicle Üniversitesi Fen Fakültesi Biyoloji Bölümü
Country: Turkey


Orcid: 0000-0002-5864-1500
Author: Mehmet Cemal TALAY
Institution: Dicle Üniversitesi Fen Bilimleri Enstitüsü
Country: Turkey


Orcid: 0000-0002-1144-047X
Author: Vedat KARACA
Institution: Zirai Mücadele Araştırma Enstitüsü
Country: Turkey


Supporting Institution Dicle üniversitesi
Project Number FEN. 18.002
Thanks Çalışmayı maddi kaynak sağlayarak destekleyen (Proje No: FEN. 18.002 ) Dicle üniversitesi Bilimsel Araştırma Projeleri (DÜBAP) Koordinatörlüğüne çok teşekkür ederiz.
Dates

Application Date : February 6, 2020
Acceptance Date : April 9, 2020
Publication Date : October 31, 2020

APA Başhan, M , Talay, M , Karaca, V . (2020). Süne, Eurygaster integriceps’in Put. (Heteroptera: Scutelleridae) Diyapoz Öncesi ve Diyapoz Sonrası Erginlerinden Hazırlanan Fosfolipit Altsınıflarının Yağ Asidi Bileşiminindeki Değişiklikler . Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 23 (5) , 1314-1321 . DOI: 10.18016/ksutarimdoga.vi.685815