Year 2020, Volume 23 , Issue 5, Pages 1119 - 1124 2020-10-31

In vitro Cytotoxic Effect of Ethanol and Dimethyl Sulfoxide on Various Human Cell Lines
Etanol ve Dimetil Sülfoksidin Çeşitli İnsan Hücre Hatları Üzerindeki in vitro Sitotoksik Etkisi

Elif AYAZOGLU DEMİR [1] , Selim DEMİR [2] , Yüksel ALİYAZICIOĞLU [3]


Although the cytotoxic effects of ethanol and dimethyl sulfoxide (DMSO) on some cell lines have been shown in the literature, there is no study about the cytotoxic effects of these solvents on common used human cell lines, such as melanoma (VMM917), lung cancer (A549), colon cancer (WiDr), normal colon (CCD 841 CoN) and fibroblast (BJ) cells. The aim of this study was to determine the cytotoxic effects of ethanol and DMSO on human breast (MCF-7), liver (HepG2) and cervix (HeLa) cancer cells in addition to above mentioned cells. For this purpose, the cells were treated with different concentrations (0.1%, 0.2%, 0.4%, 0.6%, 0.8%, 1%, 2%, and 4%, v/v) of ethanol and DMSO and then subjected to MTT assay. According to the results, ethanol and DMSO exhibited dose-dependent cytotoxicity in all cells studied. The most DMSO and ethanol sensitive cells were WiDr and VMM917, while the most resistant cells were determined as BJ and A549, respectively. The results revealed that the concentration range in which ethanol and DMSO exhibited cytotoxic effect in each cell line is different. In order not to cause false positive and negative results, the concentration range in which the solvents used in cell culture studies do not have cytotoxic effects should be determined.
Literatürde etanol ve dimetil sülfoksit (DMSO)’in bazı hücre hatları üzerindeki sitotoksik etkileri gösterilmiş olmasına rağmen, bu çözücülerin melanoma (VMM917), akciğer kanseri (A549), kolon kanseri (WiDr), normal kolon (CCD 841 CoN) ve fibroblast (BJ) hücreleri üzerindeki sitotoksik etkilerini belirleyen bir çalışma bulunmamaktadır. Bu çalışmanın amacı, etanol ve DMSO'nun yukarıda belirtilen hücrelere ek olarak insan meme (MCF-7), karaciğer (HepG2) ve serviks (HeLa) kanser hücreleri üzerindeki sitotoksik etkilerini belirlemektir. Bu amaçla, hücreler farklı konsantrasyonlarda (%0.1, %0.2, %0.4, %0.6, %0.8, %1, %2 ve % 4, hacim/hacim) etanol ve DMSO ile muamele edilmiş ve daha sonra MTT prosedürü uygulanmıştır. Elde edilen sonuçlar, etanol ve DMSO’nun incelenen tüm hücrelerde doza bağlı sitotoksisite sergilediğini göstermiştir. DMSO ve etanole en duyarlı hücreler sırasıyla WiDr ve VMM917 iken, en dirençli hücreler sırasıyla BJ ve A549 olarak belirlenmiştir. Sonuçlar, her bir hücre hattında etanol ve DMSO'nun sitotoksik etki sergilediği konsantrasyon aralığının farklı olduğunu ortaya koymuştur. Yanlış pozitif ve negatif sonuçlara neden olmamak için, hücre kültürü çalışmalarında öncelikle kullanılacak çözücülerin sitotoksik etkilerinin oluşmadığı konsantrasyon aralığı belirlenmelidir.
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Primary Language en
Subjects Basic Sciences
Journal Section RESEARCH ARTICLE
Authors

Orcid: 0000-0001-9027-7633
Author: Elif AYAZOGLU DEMİR
Institution: KARADENİZ TEKNİK ÜNİVERSİTESİ
Country: Turkey


Orcid: 0000-0002-1863-6280
Author: Selim DEMİR (Primary Author)
Institution: Karadeniz Teknik Üniversitesi Sağlık Bilimleri Fakültesi Beslenme ve Diyetetik Bölümü
Country: Turkey


Orcid: 0000-0001-9474-4307
Author: Yüksel ALİYAZICIOĞLU
Institution: KARADENİZ TEKNİK ÜNİVERSİTESİ
Country: Turkey


Dates

Application Date : March 12, 2020
Acceptance Date : April 24, 2020
Publication Date : October 31, 2020

APA Ayazoglu Demi̇r, E , Demi̇r, S , Ali̇yazıcıoğlu, Y . (2020). In vitro Cytotoxic Effect of Ethanol and Dimethyl Sulfoxide on Various Human Cell Lines . Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 23 (5) , 1119-1124 . DOI: 10.18016/ksutarimdoga.vi.702702