Year 2020, Volume 23 , Issue 6, Pages 1678 - 1686 2020-12-31

Simental Irkı Sığırlarda GH ve PIT-I Gen Polimorfizmleri ile Süt Verimleri Arasındaki İlişkinin Araştırılması
Investigation of the Relationship between GH and PIT-I Gene Polymorphisms and Milk Yields in Simmental Cattle Breed

Mustafa KORKMAZ [1] , Bilal AKYÜZ [2]


Bu çalışmada Simental ırkı sığırlarda büyüme hormonu (GH) ve hipofiz spesifik transkripsiyon faktör-1 (PIT-1) genleri ile 1. ve 2. laktasyon toplam süt verimleri ve günlük süt verimleri arasındaki ilişkinin araştırılması amaçlanmıştır. Çalışmada, Kayseri ili civarında yetiştirilen 203 baş Simental ırkı sağmal inek incelenmiştir. GH genotiplerinin belirlenmesi amacıyla yapılan PCR işlemi sonunda elde edilen 223 bp’lik PCR ürünleri AluI restriksiyon enzimiyle kesilmiştir. PIT-1 genotiplerinin belirlenmesi amacıyla yapılan PCR işlemi sonunda elde edilen 600 bp’lik PCR ürünleri ise HinfI enzimi ile kesilmiştir. GH-AluI ve PIT-1-HinfI genotipleri ile günlük ve laktasyon toplam süt verimleri arasındaki ilişki tek yönlü varyans analizi (ANOVA) ile değerlendirilmiştir. GH-AluI polimorfizmi yönünden incelenen Simental ırkı sığırlarda iki allel (A ve B) ile üç genotip (LL, LV ve VV) gözlenmiştir. İncelenen örneklerde LL genotip frekasının en yüksek (0.52), VV genotip frekansının ise en düşük (0.11) olduğu belirlenmiştir. PIT-1-HinfI polimorfizmi yönünden incelenen Simental ırkı ineklerde iki genotip (AB ve BB) gözlenmiştir. AA genotipine incelenen örneklerde rastlanılmamıştır. İncelenen Simental ırkı sığırlarda BB genotip frekasının en yüksek (0.74) olduğu belirlenmiştir. Çalışma sonunda Simental ırkı ineklerde GH-AluI ve PIT-1-HinfI polimorfizmleri ile 1., 2. laktasyon toplam süt verimleri ve günlük süt verimi arasında istatistik olarak önemli ilişki olmadığı belirlenmiştir (P>0.05). Simental ırkı sığırlarda GH-AluI ve PIT-1-HinfI polimorfizmleri ile diğer süt verim özellikleri ile büyüme ve et verim özellikleri arasındaki ilişkilerin araştırıldığı çalışmaların planlanması gerektiği düşünülmüştür.

The aim of this study was to investigate the relationship between growth hormone (GH) and pituitary-specific transcription factor-1 (PIT-1) genes and 1th, 2nd lactation total milk yield and daily milk yield in Simental cattle breed. In this study 203 Simmental dairy cows, reared in Kayseri vicinity, were examined. In order to determine GH genotypes, 223 bp PCR products obtained by PCR were digested with AluI restriction enzyme. In order to determine PIT-1 genotypes, 600 bp PCR products obtained by PCR were digested with HinfI restriction enzyme. The mean differences of 305-day milk yield among the genotype groups of GH-AluI and PIT-1-HinfI were assessed by one-way analysis of variance (ANOVA). Two alleles (A and B) and three genotypes (AA, AB and BB) for GH-AluI polymorphism were observed in examined Simmental cows. The LL genotype frequency was found highest (0.52) and the VV genotype frequency was found the lowest (0.11) in examined Simmental cows. The frequency of the L allele was found higher (0.7) than V allele (0.3) in this study. Two alleles (A and B) and two genotypes (AB and BB) for PIT-1-HinfI polymorphism were observed in examined Simmental cows in this study. The AA genotype was not found in the examined samples. The BB genotype frequency was found to be highest (0.74) in examined Simnetal cows. End of the study, the genotype effects for GH-AluI and PIT-1-HinfI polymorphisms on daily and lactation total milk yield were not found to be significant in examined Simmental cows (P>0.05). It was thought that further studies on the association between GH-AluI and PIT-1-HinfI polymorphism and other milk yield traits and meat yield traits in Simmental cattle breed should be conducted.

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Primary Language tr
Subjects Agriculture
Journal Section RESEARCH ARTICLE
Authors

Orcid: 0000-0002-4108-914X
Author: Mustafa KORKMAZ
Institution: ERCİYES ÜNİVERSİTESİ, SAĞLIK BİLİMLERİ ENSTİTÜSÜ, ZOOTEKNİ (VETERİNER) (YL) (TEZLİ)
Country: Turkey


Orcid: 0000-0001-7548-9830
Author: Bilal AKYÜZ (Primary Author)
Institution: ERCİYES ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, ZOOTEKNİ VE HAYVAN BESLEME BÖLÜMÜ, VETERİNERLİK GENETİĞİ ANABİLİM DALI
Country: Turkey


Supporting Institution Erciyes Üniversitesi Bilimsel Araştırma Projeleri Birimi
Project Number TYL-2019-8822
Thanks Bu çalışmayı TYL-2019-8822 proje kodu ile destekleyen Erciyes Üniversitesi Bilimsel Araştırma Projeleri Birimi’ne teşekkür ederiz.
Dates

Application Date : January 14, 2020
Acceptance Date : May 15, 2020
Publication Date : December 31, 2020

APA Korkmaz, M , Akyüz, B . (2020). Simental Irkı Sığırlarda GH ve PIT-I Gen Polimorfizmleri ile Süt Verimleri Arasındaki İlişkinin Araştırılması . Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 23 (6) , 1678-1686 . DOI: 10.18016/ksutarimdoga.vi.674618