Simental Irkı Sığırlarda GH ve PIT-I Gen Polimorfizmleri ile Süt Verimleri Arasındaki İlişkinin Araştırılması

Mustafa Korkmaz; Bilal Akyüz

doi:10.18016/ksutarimdoga.vi.674618

EN TR

Investigation of the Relationship between GH and PIT-I Gene Polymorphisms and Milk Yields in Simmental Cattle Breed

Abstract

The aim of this study was to investigate the relationship between growth hormone (GH) and pituitary-specific transcription factor-1 (PIT-1) genes and 1th, 2nd lactation total milk yield and daily milk yield in Simental cattle breed. In this study 203 Simmental dairy cows, reared in Kayseri vicinity, were examined. In order to determine GH genotypes, 223 bp PCR products obtained by PCR were digested with AluI restriction enzyme. In order to determine PIT-1 genotypes, 600 bp PCR products obtained by PCR were digested with HinfI restriction enzyme. The mean differences of 305-day milk yield among the genotype groups of GH-AluI and PIT-1-HinfI were assessed by one-way analysis of variance (ANOVA). Two alleles (A and B) and three genotypes (AA, AB and BB) for GH-AluI polymorphism were observed in examined Simmental cows. The LL genotype frequency was found highest (0.52) and the VV genotype frequency was found the lowest (0.11) in examined Simmental cows. The frequency of the L allele was found higher (0.7) than V allele (0.3) in this study. Two alleles (A and B) and two genotypes (AB and BB) for PIT-1-HinfI polymorphism were observed in examined Simmental cows in this study. The AA genotype was not found in the examined samples. The BB genotype frequency was found to be highest (0.74) in examined Simnetal cows. End of the study, the genotype effects for GH-AluI and PIT-1-HinfI polymorphisms on daily and lactation total milk yield were not found to be significant in examined Simmental cows (P>0.05). It was thought that further studies on the association between GH-AluI and PIT-1-HinfI polymorphism and other milk yield traits and meat yield traits in Simmental cattle breed should be conducted.

Keywords

GH,Milk yield,PIT-1,RFLP,Simental

Simental Irkı Sığırlarda GH ve PIT-I Gen Polimorfizmleri ile Süt Verimleri Arasındaki İlişkinin Araştırılması

Öz

Bu çalışmada Simental ırkı sığırlarda büyüme hormonu (GH) ve hipofiz spesifik transkripsiyon faktör-1 (PIT-1) genleri ile 1. ve 2. laktasyon toplam süt verimleri ve günlük süt verimleri arasındaki ilişkinin araştırılması amaçlanmıştır. Çalışmada, Kayseri ili civarında yetiştirilen 203 baş Simental ırkı sağmal inek incelenmiştir. GH genotiplerinin belirlenmesi amacıyla yapılan PCR işlemi sonunda elde edilen 223 bp’lik PCR ürünleri AluI restriksiyon enzimiyle kesilmiştir. PIT-1 genotiplerinin belirlenmesi amacıyla yapılan PCR işlemi sonunda elde edilen 600 bp’lik PCR ürünleri ise HinfI enzimi ile kesilmiştir. GH-AluI ve PIT-1-HinfI genotipleri ile günlük ve laktasyon toplam süt verimleri arasındaki ilişki tek yönlü varyans analizi (ANOVA) ile değerlendirilmiştir. GH-AluI polimorfizmi yönünden incelenen Simental ırkı sığırlarda iki allel (A ve B) ile üç genotip (LL, LV ve VV) gözlenmiştir. İncelenen örneklerde LL genotip frekasının en yüksek (0.52), VV genotip frekansının ise en düşük (0.11) olduğu belirlenmiştir. PIT-1-HinfI polimorfizmi yönünden incelenen Simental ırkı ineklerde iki genotip (AB ve BB) gözlenmiştir. AA genotipine incelenen örneklerde rastlanılmamıştır. İncelenen Simental ırkı sığırlarda BB genotip frekasının en yüksek (0.74) olduğu belirlenmiştir. Çalışma sonunda Simental ırkı ineklerde GH-AluI ve PIT-1-HinfI polimorfizmleri ile 1., 2. laktasyon toplam süt verimleri ve günlük süt verimi arasında istatistik olarak önemli ilişki olmadığı belirlenmiştir (P>0.05). Simental ırkı sığırlarda GH-AluI ve PIT-1-HinfI polimorfizmleri ile diğer süt verim özellikleri ile büyüme ve et verim özellikleri arasındaki ilişkilerin araştırıldığı çalışmaların planlanması gerektiği düşünülmüştür.

Anahtar Kelimeler

GH,PIT-1,RFLP,Simental,Süt verimi

Destekleyen Kurum

Erciyes Üniversitesi Bilimsel Araştırma Projeleri Birimi

Proje Numarası

TYL-2019-8822

Teşekkür

Bu çalışmayı TYL-2019-8822 proje kodu ile destekleyen Erciyes Üniversitesi Bilimsel Araştırma Projeleri Birimi’ne teşekkür ederiz.

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Ayrıntılar

Birincil Dil

Türkçe

Konular

Ziraat, Veterinerlik ve Gıda Bilimleri

Bölüm

Araştırma Makalesi

Yazarlar

Mustafa Korkmaz
0000-0002-4108-914X
Türkiye

Bilal Akyüz ^*
0000-0001-7548-9830
Türkiye

Yayımlanma Tarihi

31 Aralık 2020

Gönderilme Tarihi

14 Ocak 2020

Kabul Tarihi

15 Mayıs 2020

Yayımlandığı Sayı

Yıl 2020 Cilt: 23 Sayı: 6

DOI

https://doi.org/10.18016/ksutarimdoga.vi.674618

IZ

https://izlik.org/JA37SB78TS

Kaynak Göster

RIS / Bibtex

APA

Korkmaz, M., & Akyüz, B. (2020). Simental Irkı Sığırlarda GH ve PIT-I Gen Polimorfizmleri ile Süt Verimleri Arasındaki İlişkinin Araştırılması. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 23(6), 1678-1686. https://doi.org/10.18016/ksutarimdoga.vi.674618