Year 2020, Volume 23 , Issue 6, Pages 1495 - 1502 2020-12-31

Diyabetik Ratlarda Zakkum (Nerium oleander L.) Çiçeği Etanolik Liyofilize Ekstresinin Eritrosit Frajilite, Hematolojik ve Antioksidan Etkilerinin Araştırılması
Investigation of the Erythrocyte Fragility, Hematological and Antioxidant Effects of Oleander (Nerium oleander L.) Flower Ethanolic Lyophilized Extract in Diabetic Rats

Abdulahad DOĞAN [1]


Bu çalışma streptozotosin (50 mg kg-1) ile Tip 1 diyabet oluşturulan ratlarda zakkum (N. oleander) çiçeğinden elde edilen etanolik liyofilize ekstresinin eritrosit frajilite, hematolojik parametreler, total antioksidan kapasite (TAS), total oksidan kapasite (TOS) ve oksidatif stress indeksi (OSI) üzerine olan etkilerine odaklandı. Toksisite testi sonrası, 49 rat 7 gruba ayrıldı. Kontrol, Nerium oleander (NO) (50 mg kg-1), Diabetes mellitus (DM), DM+Gly-3 (3 mg kg-1), DM+NO-25 (25 mg kg-1), DM+NO-75 (75 mg kg-1) ve DM+NO-225 (225 mg kg-1). 21 günlük uygulamadan sonra elde edilen bulgulara göre, DM grubunda eritrosit hemolizi (%0.2-0.6 NaCI konsantrasyonlarda) kontrol gruplarına göre önemli artış gösterirken (P<0.05); özellikle DM+NO-225 grubunda eritrosit hemolizi önemli oranda azaldı (P<0.05). Hematolojik parametreler, bazı gruplarda dalgalanmalar göstermesine rağmen, DM+Gly grubunda eritrosit (RBC), hemoglobin (HGB) ve hematokrit (HCT) düzeyleri diğer gruplara göre önemli artış gösterdi (P<0.05). Ayrıca, DM grubu lökosit (WBC), eritrosit dağılım genişliği (RDW) ve ortalama eritrosit hacmi (MCV) değerleri kontrol gruplarına göre önemli artış gösterirken, eritrosit ortalama hemoglobin konsantrasyonu (MCHC) ise önemli oranda azaldı (P<0.05). DM grubunda plazma TAS seviyesindeki azalma, ile TOS ve OSI değerindeki artış kontrol ve uygulama gruplarına göre önemli bulundu. N. oleander çiçek liyofilize ekstresinin STZ ile diyabet oluşturulan ratlarda diyabetin sebep olduğu artan hemolizi önleyici/azaltıcı etkilere ve antioksidan özelliklere sahip olabileceği sonucuna varılmıştır. 

The present study was focused on the erythrocyte fragility, hematological parameters, total antioxidant capacity (TAS), total oxidant capacity (TOS) and oxidative stress index (OSI) of flower ethanolic lyophilized extract obtained from N. oleander plant on Streptozotocin (50 mg kg-1)-induced Type 1 diabetes in rats. After toxicity test, 49 rats were divided into 7 groups. Control, Nerium oleander (NO) (50 mg kg-1), Diabetes mellitus (DM), DM+Gly-3 (3 mg kg-1), DM+NO-25 (25 mg kg-1), DM+NO-75 (75 mg kg-1) and DM+NO-225 (225 mg kg-1). Based on the results, after 21 days of application, erythrocyte hemolysis of DM group was increased (at 0.2-0.6% NaCI concentrations) compared to Control groups whereas treatment groups significantly decreased (P<0.05) erythrocyte hemolysis, especially in DM+NO-225 group. Although, hematological parameters showed some fluctuations in the groups, DM+Gly group showed significant increase (P<0.05) in erythrocyte (RBCs), hemoglobin (HGB), hematocrit (HCT) levels compared to all other groups. In addition, leukocyte (WBC), red cell distribution width (RDWs) and mean corpuscular volume (MCV) values of DM group increased with respect to the control groups whereas mean cell corpuscular hemoglobin concentration (MCHC) value significantly decreased (P<0.05). The decrease in plasma TAS level and increase in TOS and OSI values in the DM group were found significant compared to control and application groups. It was concluded that ethanolic lyophilized extract of N. oleander flowers prevented/decreased increased hemolysis in STZ-induced diabetic rats and also it could possess antioxidant properties.

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Primary Language tr
Subjects Biology
Journal Section RESEARCH ARTICLE
Authors

Orcid: 0000-0002-5438-8560
Author: Abdulahad DOĞAN (Primary Author)
Institution: Van Yüzüncü Yıl Universitesi
Country: Turkey


Supporting Institution Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Başkanlığı
Project Number TAP-2018-7098
Thanks Bu araştırma Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Başkanlığı tarafından TAP-2018-7098 numaralı proje olarak desteklenmiştir. Ayrıca bitkiyi teşhis eden sayın Doç. Dr. Süleyman Mesut PINAR hocamıza teşekkürü bir borç bilirim.
Dates

Application Date : March 31, 2020
Acceptance Date : May 14, 2020
Publication Date : December 31, 2020

APA Doğan, A . (2020). Diyabetik Ratlarda Zakkum (Nerium oleander L.) Çiçeği Etanolik Liyofilize Ekstresinin Eritrosit Frajilite, Hematolojik ve Antioksidan Etkilerinin Araştırılması . Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 23 (6) , 1495-1502 . DOI: 10.18016/ksutarimdoga.vi.712369