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The Effects of UV Irradiation and Incubation Time on in vitro Phenolic Compound Production in 'Karaerik' Grape Cultivar

Year 2020, Volume: 23 Issue: 6, 1428 - 1434, 31.12.2020

Mustafa Çelik , Nurhan Keskin , Fethi Ahmet Özdemir

https://doi.org/10.18016/ksutarimdoga.vi.714642
Cited By: 2

Abstract

In this study, the effect of Ultraviolet (UV) irradiation on induction of individual and total phenolics production on callus cultures of ‘Karaerik’ grape cultivar was investigated. Callus tissues were obtained from the leaves of the cuttings grown in in vitro plants. As a culture medium, Gamborg B-5 was utilized with 0.1 mg L-1 NAA (Naphthaleneacetic acid) and 0.2 mg L-1 Kin (Kinetin). Callus tissues were subcultured twice with 21 days intervals. After the second subculture, 12-day-old callus tissues were exposed to 254 nm UV-C light at 10 cm distance from the source for 10 and 15 min by opening covers of the petri dishes in sterile cabin. After the treatment, callus tissues were incubated under dark conditions. Phenolic compounds were measured at 0, 24th, 48th and 72nd hours. Individual phenolic compounds were analyzed by HPLC (High Pressure Liquid Chromatography) and total phenolic compounds were measured by spectrophotometer. As a result of the study, it was found that UV irradiation was effective for induction the production of phenolic compounds in the callus tissues of ‘Karaerik’ grape cultivar and this effect was closely related to the application time.

Keywords

Grapevine Analytical methods Elicitor Callus culture Secondary metabolite

Supporting Institution

Van Yüzüncü Yıl University Scientific Research Project Coordination

Project Number

FYL-2018-7285

Thanks

We are grateful for the financial support provided for this research by Van Yüzüncü Yıl University Scientific Research Project Coordination (FYL-2018-7285).

References

  • Atak A, Göksel Z 2019. Determination of Some Phenolic Substance Changes in Cultivar/Genotypes of Different Vitis Species. Ege Univ Ziraat Fak Derg 56 (2):153-161.
  • Bais AJ, Murphy PJ, Dry IB 2000. The Molecular Regulation of Stilben Phytoalexin Biosynthesis in Vitis vinifera During Grape Berry Development. Aust. J. Plant Physiol 27: 425-433.
  • Barz W, Daniel S, Hindeer W, Jaques U, Kessman H, Koster J, Otto C, Tiemann K 1988. Elicitation and Metabolism of Phytoalexins in Plant Cell Cultures. In: Application of Plant Cell and Tissue Culture, CIBA Foundation Symposium 137, Toronto, 178-198.
  • Bonello M, Gaši´c U, Teši´c Ž, Attard E 2019. Production of Stilbenes in Callus Cultures of the Maltese Indigenous Grapevine Variety, Gellewza. Molecules 24: 2112.
  • Canturk S, Kunter B, Çoksarı G 2018. Effects of Kaolin and Dicarboxylic Acid Based Stress Inhibitors on Aroma Composition of Two Table Grape Cultivars (Vitis vinifera L.). Acta Sci.Pol. Hortorum Cultus 17(5): 37-46.
  • Castro-Lopez L, Castillo-Sanchez G, D´ıaz-Rubio L, Cordova-Guerrero I 2019. Total Content of Phenols and Antioxidant Activity of Grape Skins and Seeds Cabernet Sauvignon Cultivated in Valle de Guadalupe, Baja California, Mexico. BIO Web of Conferences 15: 04001.
  • Charlwood BV, Charlwood KA, Molina-Torres J 1990. Accumulation of Secondary Compounds by Organized Plant Cultures. P: 167-300. In: Secondary Products from Plant Tissue Culture. Eds. Charlwood, B. V., Rhodes, M. J. C., Clarendon Pres, Oxford.
  • Colombo F, Di Lorenzo C, Regazzoni L, Fumagalli M, Sangiovanni E, Peres de Sousa L, Bavaresco L, Tomasi D, Bosso A, Aldini G, Restani P, Dell'Agli M 2019. Phenolic Profiles and Anti-inflammatory Activities of Sixteen Table Grape (Vitis vinifera L.) varieties. Food and Function 10(4): 1797-1807.
  • Çetin ES, Uzunlar F, Baydar NG 2011. UV-C Uygulamasının Gamay Üzüm Çeşidine Ait Kalluslarda Sekonder Metabolit Üretimi Üzerine Etkileri. Gıda 36(6): 319-326.
  • Di Lorenzo C, Colombo F, Biella S, Orgiu F, Frigerio G, Regazzoni L, de Sousa L P, Bavaresco L, Bosso A, Aldini G, Restani P 2019. Phenolic Profile and Antioxidant Activity of Different Grape (Vitis vinifera L.) varieties. BIO Web of Conferences 12: 04005.
  • Gamborg OL, Miller R, Ojima K 1968. Nutrient Requirement Suspensions Cultures of Soybean Root Cells. Experimental Cell Research 50: 151-158.
  • Gökçen İS, Keskin N, Kunter B, Cantürk S, Karadoğan B 2017. Üzüm Fitokimyasalları ve Türkiye’de Yetiştirilen Üzüm Çeşitleri Üzerindeki Araştırmalar. Turkish Journal of Forest Science: 93-111.
  • Keskin N 2019. Asmanın Kalkanı: Stilbenler. Tarım Bilimlerinde Güncel Araştırma ve Değerlendirmeler. Ed: Kunter B ve Keskin N. Stamparija Ivpe, Cetinje, 29-46 sy.
  • Keskin N, Kunter B 2007. Erciş Üzüm Çeşidinin Kallus Kültürlerinde UV Işını Etkisiyle Resveratrol Üretiminin Uyarılması. Tarım Bilimleri Dergisi 13: 379-384.
  • Keskin N, Kunter B 2008. Production of trans-resveratrol in 'Cabernet Sauvignon' (Vitis vinifera L.) Callus Culture in Response to Ultraviolet-C Irradiation. Vitis 47(4):193-196.
  • Keskin N, Kunter B 2009. The Effects of Callus Age, UV Irradiation and Incubation time on trans-resveratrol Production in Grapevine Callus Culture. Journal of Agricultural Sciences 15(1):9-13.
  • Keskin N, Kunter B 2010. Production of trans-resveratrol in Callus Tissue of Öküzgözü (Vitis vinifera L.) in Response to Ultraviolet-C Irradiation. The J. Anim. Plant. Sci 20(3): 197-200.
  • Keskin N, Kunter B 2017. Stilbenes Profile in Various Tissues of Grapevine (Vitis vinifera L. cv. ‘Ercis’). JEPE 19(3): 1259-1267.
  • Kunter B, Cantürk S, Keskin N 2013. Üzüm Tanesinin Histokimyasal Yapısı. Iğdır Üni. Fen Bilimleri Enst. Der 3(2): 17-24.
  • Liu W, Liu CY, Yang CX, Wang LJ, Li SH 2010. Effect of Grape Genotype and Tissue Type on Callus Growth and Production of Resveratrols and Their Piceids After UV-C Irradiation. Food Chem 122: 475-481.
  • López-Vélez M, Martinez F, Del Valle-Ribes C 2003. The Study of Phenolic Compounds as Natural Antioxidants in Wine. Crit. Rev. Food Sci. Nutr 43: 233-244.
  • Lutz M, Jorquera K, Cancino B, Ruby R, Henriquez C 2011. Phenolics and Antioxidant Capacity of Table Grape (Vitis vinifera L.) Cultivars Grown in Chile. J Food Sci 76:C1088-C1093.
  • Murashige T, Skoog F 1962. A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures. Physol. Plant 15: 473-497.
  • Oğuz D, Keskin N, Özdemir FA 2020. Induction of Anthocyanin Accumulation in Callus Culture of 'Karaerik' (Vitis vinifera L.) by UV Irradiation Effect. Turkish Journal of Agricultural Research, 7(1): 96-104.
  • Perestrelo R, Lu Y, Santos S, Silvestre AJD, Neto CP, Camara JS, Rocha SM 2012. Phenolic Profile of Sercial and Tinta Negra Vitis vinifera L. Grape Skins by HPLC-DAD-ESI-MSn. Food Chem 135:94-104.
  • Ribéreau-Gayon P, Gloires Y, Maujean A, Dubourdieu D 2000. Handbook of Enology Volume 2: The Chemistry of Wine and Stabilization and Treatments, John Wiley and Sons Ltd., West Sussex, England, pp 129-185.
  • Rodriguez-Delgado MA. Malovana S, Perez JP, Borges T, Garcia-Montelongo FJ 2001. Separation of Phenolic Compounds by High-Performance Liquid Chromatography with Absorbance and Fluorimetric Detection. Journal of Chromatography, 912: 249-257.
  • Rodríguez-Montealegre R, Romero-Peces R, Chacón-Vozmediano JL, Martínez-Gascueña J, García-Romero E 2006. Phenolic Compounds in Skins and Seeds of Ten Grape Vitis vinifera Varieties Grown in a Warm Climate. J Food Composi Anal 19:687-693.
  • Swain, T., Hillis, W. E., 1959. The Phenolic Constituents of Prunus domestica L. the Quantitative Analysis of Phenolic Constituents. Journal of the Science of Food and Agriculture 10: 63-68.
  • Takayanagi T, Okuda T, Mine Y, Yokotsuka K 2004. Induction of Resveratrol Biosynthesis in Skins of Three Grape Cultivars by Ultraviolet Irradiation. Journal of the Japanese Society for Horticultural Science 73(3): 193-199.
  • Versari, A., Parpinello, G. P., Tornielli, G. B., Ferrarini, R., & Giulivo, C. (2001). Stilbene compounds and stilbene synthase expression during ripening, wilting and UV treatment in grape cv. Corvina. Journal of Agricultural and Food Chemistry 49(11): 5531-5536.

‘Karaerik’ Üzüm Çeşidinde in vitro Fenolik Bileşik Üretimi Üzerine UV Işını Uygulaması ve İnkübasyon Sürelerinin Etkisi

Year 2020, Volume: 23 Issue: 6, 1428 - 1434, 31.12.2020

Mustafa Çelik , Nurhan Keskin , Fethi Ahmet Özdemir

https://doi.org/10.18016/ksutarimdoga.vi.714642
Cited By: 2

Abstract

Bu çalışmada, Karaerik üzüm çeşidine ait kallus kültürlerinde, bireysel ve toplam fenolik bileşik üretiminin uyarılması üzerine Ultraviyole (UV) ışınının etkisi incelenmiştir. Kallus kültürleri, in vitro bitkiciklerin yaprak ayalarından elde edilmiştir. Kültür ortamı olarak, 0.1 mg l-1 NAA (Naftalen asetik asit) ve 0.2 mg l-1 Kin (Kinetin) eklenmiş Gamborg B-5 ortamı kullanılmıştır. Kalluslar, 21 gün ara ile iki defa alt kültüre alınmıştır. İkinci alt kültürden sonra, 12 gün yaşlı kalluslara, steril kabin içerisinde petri kutularının kapakları açılarak, 10 cm uzaklıktan 10 ve 15 dk süreyle 254 nm dalga boyuna sahip UV-C ışını uygulanmıştır. Uygulamanın ardından kalluslar karanlık koşullarda inkübe edilmiştir. Fenolik bileşik ölçümleri 0., 24., 48. ve 72. saatlerde yapılmıştır. Bireysel fenolik bileşikler HPLC (Yüksek Basınçlı Sıvı Kromatografisi), toplam fenolik bileşikler ise Spektrofotometre ile analiz edilmiştir. Çalışma sonucunda UV ışınının Karaerik üzüm çeşidine ait kallus dokularında fenolik bileşik üretimini uyarmada etkili ve bu etkinin uygulama süresi ile yakından ilişkili olduğu saptanmıştır.

Keywords

Asma Analitik yöntemler Elisitör Kallus kültürü Sekonder metabolit

Project Number

FYL-2018-7285

References

  • Atak A, Göksel Z 2019. Determination of Some Phenolic Substance Changes in Cultivar/Genotypes of Different Vitis Species. Ege Univ Ziraat Fak Derg 56 (2):153-161.
  • Bais AJ, Murphy PJ, Dry IB 2000. The Molecular Regulation of Stilben Phytoalexin Biosynthesis in Vitis vinifera During Grape Berry Development. Aust. J. Plant Physiol 27: 425-433.
  • Barz W, Daniel S, Hindeer W, Jaques U, Kessman H, Koster J, Otto C, Tiemann K 1988. Elicitation and Metabolism of Phytoalexins in Plant Cell Cultures. In: Application of Plant Cell and Tissue Culture, CIBA Foundation Symposium 137, Toronto, 178-198.
  • Bonello M, Gaši´c U, Teši´c Ž, Attard E 2019. Production of Stilbenes in Callus Cultures of the Maltese Indigenous Grapevine Variety, Gellewza. Molecules 24: 2112.
  • Canturk S, Kunter B, Çoksarı G 2018. Effects of Kaolin and Dicarboxylic Acid Based Stress Inhibitors on Aroma Composition of Two Table Grape Cultivars (Vitis vinifera L.). Acta Sci.Pol. Hortorum Cultus 17(5): 37-46.
  • Castro-Lopez L, Castillo-Sanchez G, D´ıaz-Rubio L, Cordova-Guerrero I 2019. Total Content of Phenols and Antioxidant Activity of Grape Skins and Seeds Cabernet Sauvignon Cultivated in Valle de Guadalupe, Baja California, Mexico. BIO Web of Conferences 15: 04001.
  • Charlwood BV, Charlwood KA, Molina-Torres J 1990. Accumulation of Secondary Compounds by Organized Plant Cultures. P: 167-300. In: Secondary Products from Plant Tissue Culture. Eds. Charlwood, B. V., Rhodes, M. J. C., Clarendon Pres, Oxford.
  • Colombo F, Di Lorenzo C, Regazzoni L, Fumagalli M, Sangiovanni E, Peres de Sousa L, Bavaresco L, Tomasi D, Bosso A, Aldini G, Restani P, Dell'Agli M 2019. Phenolic Profiles and Anti-inflammatory Activities of Sixteen Table Grape (Vitis vinifera L.) varieties. Food and Function 10(4): 1797-1807.
  • Çetin ES, Uzunlar F, Baydar NG 2011. UV-C Uygulamasının Gamay Üzüm Çeşidine Ait Kalluslarda Sekonder Metabolit Üretimi Üzerine Etkileri. Gıda 36(6): 319-326.
  • Di Lorenzo C, Colombo F, Biella S, Orgiu F, Frigerio G, Regazzoni L, de Sousa L P, Bavaresco L, Bosso A, Aldini G, Restani P 2019. Phenolic Profile and Antioxidant Activity of Different Grape (Vitis vinifera L.) varieties. BIO Web of Conferences 12: 04005.
  • Gamborg OL, Miller R, Ojima K 1968. Nutrient Requirement Suspensions Cultures of Soybean Root Cells. Experimental Cell Research 50: 151-158.
  • Gökçen İS, Keskin N, Kunter B, Cantürk S, Karadoğan B 2017. Üzüm Fitokimyasalları ve Türkiye’de Yetiştirilen Üzüm Çeşitleri Üzerindeki Araştırmalar. Turkish Journal of Forest Science: 93-111.
  • Keskin N 2019. Asmanın Kalkanı: Stilbenler. Tarım Bilimlerinde Güncel Araştırma ve Değerlendirmeler. Ed: Kunter B ve Keskin N. Stamparija Ivpe, Cetinje, 29-46 sy.
  • Keskin N, Kunter B 2007. Erciş Üzüm Çeşidinin Kallus Kültürlerinde UV Işını Etkisiyle Resveratrol Üretiminin Uyarılması. Tarım Bilimleri Dergisi 13: 379-384.
  • Keskin N, Kunter B 2008. Production of trans-resveratrol in 'Cabernet Sauvignon' (Vitis vinifera L.) Callus Culture in Response to Ultraviolet-C Irradiation. Vitis 47(4):193-196.
  • Keskin N, Kunter B 2009. The Effects of Callus Age, UV Irradiation and Incubation time on trans-resveratrol Production in Grapevine Callus Culture. Journal of Agricultural Sciences 15(1):9-13.
  • Keskin N, Kunter B 2010. Production of trans-resveratrol in Callus Tissue of Öküzgözü (Vitis vinifera L.) in Response to Ultraviolet-C Irradiation. The J. Anim. Plant. Sci 20(3): 197-200.
  • Keskin N, Kunter B 2017. Stilbenes Profile in Various Tissues of Grapevine (Vitis vinifera L. cv. ‘Ercis’). JEPE 19(3): 1259-1267.
  • Kunter B, Cantürk S, Keskin N 2013. Üzüm Tanesinin Histokimyasal Yapısı. Iğdır Üni. Fen Bilimleri Enst. Der 3(2): 17-24.
  • Liu W, Liu CY, Yang CX, Wang LJ, Li SH 2010. Effect of Grape Genotype and Tissue Type on Callus Growth and Production of Resveratrols and Their Piceids After UV-C Irradiation. Food Chem 122: 475-481.
  • López-Vélez M, Martinez F, Del Valle-Ribes C 2003. The Study of Phenolic Compounds as Natural Antioxidants in Wine. Crit. Rev. Food Sci. Nutr 43: 233-244.
  • Lutz M, Jorquera K, Cancino B, Ruby R, Henriquez C 2011. Phenolics and Antioxidant Capacity of Table Grape (Vitis vinifera L.) Cultivars Grown in Chile. J Food Sci 76:C1088-C1093.
  • Murashige T, Skoog F 1962. A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures. Physol. Plant 15: 473-497.
  • Oğuz D, Keskin N, Özdemir FA 2020. Induction of Anthocyanin Accumulation in Callus Culture of 'Karaerik' (Vitis vinifera L.) by UV Irradiation Effect. Turkish Journal of Agricultural Research, 7(1): 96-104.
  • Perestrelo R, Lu Y, Santos S, Silvestre AJD, Neto CP, Camara JS, Rocha SM 2012. Phenolic Profile of Sercial and Tinta Negra Vitis vinifera L. Grape Skins by HPLC-DAD-ESI-MSn. Food Chem 135:94-104.
  • Ribéreau-Gayon P, Gloires Y, Maujean A, Dubourdieu D 2000. Handbook of Enology Volume 2: The Chemistry of Wine and Stabilization and Treatments, John Wiley and Sons Ltd., West Sussex, England, pp 129-185.
  • Rodriguez-Delgado MA. Malovana S, Perez JP, Borges T, Garcia-Montelongo FJ 2001. Separation of Phenolic Compounds by High-Performance Liquid Chromatography with Absorbance and Fluorimetric Detection. Journal of Chromatography, 912: 249-257.
  • Rodríguez-Montealegre R, Romero-Peces R, Chacón-Vozmediano JL, Martínez-Gascueña J, García-Romero E 2006. Phenolic Compounds in Skins and Seeds of Ten Grape Vitis vinifera Varieties Grown in a Warm Climate. J Food Composi Anal 19:687-693.
  • Swain, T., Hillis, W. E., 1959. The Phenolic Constituents of Prunus domestica L. the Quantitative Analysis of Phenolic Constituents. Journal of the Science of Food and Agriculture 10: 63-68.
  • Takayanagi T, Okuda T, Mine Y, Yokotsuka K 2004. Induction of Resveratrol Biosynthesis in Skins of Three Grape Cultivars by Ultraviolet Irradiation. Journal of the Japanese Society for Horticultural Science 73(3): 193-199.
  • Versari, A., Parpinello, G. P., Tornielli, G. B., Ferrarini, R., & Giulivo, C. (2001). Stilbene compounds and stilbene synthase expression during ripening, wilting and UV treatment in grape cv. Corvina. Journal of Agricultural and Food Chemistry 49(11): 5531-5536.
There are 31 citations in total.

Details

Primary Language English
Subjects Agricultural, Veterinary and Food Sciences
Journal Section RESEARCH ARTICLE
Authors

Mustafa Çelik 0000-0002-8251-5213

Nurhan Keskin 0000-0003-2332-1459

Fethi Ahmet Özdemir 0000-0003-0862-9690

Project Number FYL-2018-7285
Publication Date December 31, 2020
Submission Date April 4, 2020
Acceptance Date June 4, 2020
Published in Issue Year 2020Volume: 23 Issue: 6

Cite

APA Çelik, M., Keskin, N., & Özdemir, F. A. (2020). The Effects of UV Irradiation and Incubation Time on in vitro Phenolic Compound Production in ’Karaerik’ Grape Cultivar. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 23(6), 1428-1434. https://doi.org/10.18016/ksutarimdoga.vi.714642

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