Year 2020, Volume 23 , Issue 6, Pages 1428 - 1434 2020-12-31

‘Karaerik’ Üzüm Çeşidinde in vitro Fenolik Bileşik Üretimi Üzerine UV Işını Uygulaması ve İnkübasyon Sürelerinin Etkisi
The Effects of UV Irradiation and Incubation Time on in vitro Phenolic Compound Production in 'Karaerik' Grape Cultivar

Mustafa ÇELİK [1] , Nurhan KESKİN [2] , Fethi Ahmet ÖZDEMİR [3]


Bu çalışmada, Karaerik üzüm çeşidine ait kallus kültürlerinde, bireysel ve toplam fenolik bileşik üretiminin uyarılması üzerine Ultraviyole (UV) ışınının etkisi incelenmiştir. Kallus kültürleri, in vitro bitkiciklerin yaprak ayalarından elde edilmiştir. Kültür ortamı olarak, 0.1 mg l-1 NAA (Naftalen asetik asit) ve 0.2 mg l-1 Kin (Kinetin) eklenmiş Gamborg B-5 ortamı kullanılmıştır. Kalluslar, 21 gün ara ile iki defa alt kültüre alınmıştır. İkinci alt kültürden sonra, 12 gün yaşlı kalluslara, steril kabin içerisinde petri kutularının kapakları açılarak, 10 cm uzaklıktan 10 ve 15 dk süreyle 254 nm dalga boyuna sahip UV-C ışını uygulanmıştır. Uygulamanın ardından kalluslar karanlık koşullarda inkübe edilmiştir. Fenolik bileşik ölçümleri 0., 24., 48. ve 72. saatlerde yapılmıştır. Bireysel fenolik bileşikler HPLC (Yüksek Basınçlı Sıvı Kromatografisi), toplam fenolik bileşikler ise Spektrofotometre ile analiz edilmiştir. Çalışma sonucunda UV ışınının Karaerik üzüm çeşidine ait kallus dokularında fenolik bileşik üretimini uyarmada etkili ve bu etkinin uygulama süresi ile yakından ilişkili olduğu saptanmıştır.

In this study, the effect of Ultraviolet (UV) irradiation on induction of individual and total phenolics production on callus cultures of ‘Karaerik’ grape cultivar was investigated. Callus tissues were obtained from the leaves of the cuttings grown in in vitro plants. As a culture medium, Gamborg B-5 was utilized with 0.1 mg L-1 NAA (Naphthaleneacetic acid) and 0.2 mg L-1 Kin (Kinetin). Callus tissues were subcultured twice with 21 days intervals. After the second subculture, 12-day-old callus tissues were exposed to 254 nm UV-C light at 10 cm distance from the source for 10 and 15 min by opening covers of the petri dishes in sterile cabin. After the treatment, callus tissues were incubated under dark conditions. Phenolic compounds were measured at 0, 24th, 48th and 72nd hours. Individual phenolic compounds were analyzed by HPLC (High Pressure Liquid Chromatography) and total phenolic compounds were measured by spectrophotometer. As a result of the study, it was found that UV irradiation was effective for induction the production of phenolic compounds in the callus tissues of ‘Karaerik’ grape cultivar and this effect was closely related to the application time.

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Primary Language en
Subjects Agriculture
Journal Section RESEARCH ARTICLE
Authors

Orcid: 0000-0002-8251-5213
Author: Mustafa ÇELİK
Institution: VAN YÜZÜNCÜ YIL ÜNİVERSİTESİ
Country: Turkey


Orcid: 0000-0003-2332-1459
Author: Nurhan KESKİN (Primary Author)
Institution: VAN YÜZÜNCÜ YIL ÜNİVERSİTESİ
Country: Turkey


Orcid: 0000-0003-0862-9690
Author: Fethi Ahmet ÖZDEMİR
Institution: BİNGÖL ÜNİVERSİTESİ
Country: Turkey


Supporting Institution Van Yüzüncü Yıl University Scientific Research Project Coordination
Project Number FYL-2018-7285
Thanks We are grateful for the financial support provided for this research by Van Yüzüncü Yıl University Scientific Research Project Coordination (FYL-2018-7285).
Dates

Application Date : April 4, 2020
Acceptance Date : June 4, 2020
Publication Date : December 31, 2020

APA Çeli̇k, M , Keski̇n, N , Özdemi̇r, F . (2020). The Effects of UV Irradiation and Incubation Time on in vitro Phenolic Compound Production in 'Karaerik' Grape Cultivar . Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 23 (6) , 1428-1434 . DOI: 10.18016/ksutarimdoga.vi.714642