The Effects of UV Irradiation and Incubation Time on in vitro Phenolic Compound Production in 'Karaerik' Grape Cultivar
Abstract
In this study, the effect of Ultraviolet (UV) irradiation on induction of individual and total phenolics production on callus cultures of ‘Karaerik’ grape cultivar was investigated. Callus tissues were obtained from the leaves of the cuttings grown in in vitro plants. As a culture medium, Gamborg B-5 was utilized with 0.1 mg L-1 NAA (Naphthaleneacetic acid) and 0.2 mg L-1 Kin (Kinetin). Callus tissues were subcultured twice with 21 days intervals. After the second subculture, 12-day-old callus tissues were exposed to 254 nm UV-C light at 10 cm distance from the source for 10 and 15 min by opening covers of the petri dishes in sterile cabin. After the treatment, callus tissues were incubated under dark conditions. Phenolic compounds were measured at 0, 24th, 48th and 72nd hours. Individual phenolic compounds were analyzed by HPLC (High Pressure Liquid Chromatography) and total phenolic compounds were measured by spectrophotometer. As a result of the study, it was found that UV irradiation was effective for induction the production of phenolic compounds in the callus tissues of ‘Karaerik’ grape cultivar and this effect was closely related to the application time.
Keywords
Destekleyen Kurum
Proje Numarası
Teşekkür
Kaynakça
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Ayrıntılar
Birincil Dil
İngilizce
Konular
Ziraat, Veterinerlik ve Gıda Bilimleri
Bölüm
Araştırma Makalesi
Yayımlanma Tarihi
31 Aralık 2020
Gönderilme Tarihi
4 Nisan 2020
Kabul Tarihi
4 Haziran 2020
Yayımlandığı Sayı
Yıl 2020 Cilt: 23 Sayı: 6
Cited By
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Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi
https://doi.org/10.18016/ksutarimdoga.vi.1241907