Year 2020, Volume 23 , Issue 6, Pages 1474 - 1482 2020-12-31

Chemical Composition and Antibacterial Activity of Essential Oils Isolated from Medicinal Plants against Gall Forming Plant Pathogenic Bacterial Disease Agents
Tıbbi Bitkilerden Elde Edilen Uçucu Yağların Kimyasal Bileşimi ve Gal Oluşturan Bitki Patojeni Bakteriyel Hastalık Etmenlerine Karşı Antibakteriyel Etkinlikleri

İ̇mam Adem BOZKURT [1] , Soner SOYLU [2] , Merve KARA [3] , Emine Mine SOYLU [4]


The aims of this study were to determine chemical composition of essential oils from different plant species and their antibacterial activities against gal-forming plant pathogenic bacterial disease agents Rhizobium radiobacter, Pseudomonas savastanoi pv. savastanoi and P.savastanoi pv. nerii. The chemical compositions of essential oils were identified by GC/MS. The major constituents of the essential oils were carvacrol in Thymbra spicata var. spicata (66.88%) and Origanum syriacum (79.8%), thymol in Thymus serpyllum (41.03%), geranial in Thymus sipyleus (13.72%) and Melissa officinalis (30.4), 4-terpineol in Origanum majorana (31.67%), linalool in Ocimum basilicum (30.23%), carvone in Mentha spicata (55.58%), 1,8 cineole in Lavandula stoechas var. stoechas (35.5%), Laurus nobilis (35.5%) and Rosmarinus officinalis (18.47%), camphor in Salvia officinalis (24.59%) and trans-anethole in Foeniculum vulgare (82.8%) essential oils, respectively. Based on inhibition zone diameter values, essential oils showed very strong antibacterial activities against P.savastanoi pv. savastanoi (7.0-44.67 mm), followed by R.radiobacter (9.6-37.67 mm) and P.savastanoi pv. nerii (6.33-18.33 mm). Essential oils of plants belong to Lamiaceae family were generally found to be more efficient than those belong to Lauraceae and Apiaceae families. The essential oils of O.syriacum, T.serpyllum and T.spicata var. spicata were found to be the most promising essential oils displaying the highest antibacterial activities against all tested bacterial species. The findings of the present study revealed that essential oils have a potential to be used as antibacterial agents against gall forming bacterial disease agents.
Bu çalışmanın amacı, farklı bitki türlerinden elde edilen uçucu yağların kimyasal bileşimini ve gal (ur) oluşturan bitki patojenik bakteriyel hastalık etmeni Rhizobium radiobacter, Pseudomonas savastanoi pv. savastanoi ve P.savastanoi pv. nerii’ye karşı antibakteriyel etkinlerini belirlemektir. Thymbra spicata var. spicata, Thymus serpyllum, Thymus sipyleus, Origanum syriacum, Origanum majorana, Ocimum basilicum, Mentha spicata, Melissa officinalis, Lavandula stoechas var. stoechas, Rosmarinus officinalis, Salvia officinalis, Laurus nobilis ve Foeniculum vulgare uçucu yağlarının kimyasal bileşenleri GC/MS ile tanımlanmıştır. Carvacrol Thymbra spicata var. spicata (% 66.88) ve Origanum syriacum (% 79.8), thymol Thymus serpyllum (% 41.03), geranial Thymus sipyleus (% 13.72) ve Melissa officinalis (30.4), 4-terpineol Origanum majorana (31.67 %), linalool Ocimum basilicum (% 30.23), carvone Mentha spicata (% 55.58), 1,8 cineole Lavandula stoechas var. stoechas (% 35.5), Laurus nobilis (% 35.5) ve Rosmarinus officinalis (% 18.47), camphor Salvia officinalis (% 24.59) ve trans-anethole ise Foeniculum vulgare (% 82.8) uçucu yağlarının ana bileşenleri olarak belirlenmiştir. Engelleme zon çapı değerlerine dayanarak, uçucu yağlarıP.savastanoi pv. savastanoi (7.0-44.67 mm), ardından R.radiobacter (9.6-37.67 mm) ve P.savastanoi pv. nerii (6.33-18.33 mm) ye karşı oldukça güçlü antibakteriyel etkinlik göstermiştir. Lamiaceae familyasına ait bitkilerin uçucu yağlarının genellikle Lauraceae ve Apiaceae familyalarına göre daha etkili olduğu bulunmuştur. O.syriacum, T.serpyllum ve T.spicata var. spicata, test edilen tüm bakteri türlerine karşı en yüksek antibakteriyel aktiviteleri gösteren umut verici uçucu yağlar olarak belirlenmiştir. Bu çalışmanın bulguları uçucu yağların gal oluşturan bakteriyel hastalık etmenlerine karşı antibakteriyel ajan olarak kullanılma potansiyeline sahip olduğunu göstermiştir.
  • Adams R 2001. Essential Oil Components by Quadrupole GC/MS. Allured Publishing Corp. Carol Stream, IL
  • Agrios G 2005. Plant Pathology. San Diego, CA: Elsevier.
  • Aktan ZC, Soylu S 2020. Diyarbakır İlinde Yetişen Badem Ağaçlarından Endofit ve Epifit Bakteri Türlerinin İzolasyonu ve Bitki Gelişimini Teşvik Eden Mekanizmalarının Karakterizasyonu. KSÜ Tarım ve Doğa Derg 23 (3): 641-654.
  • Ateeq-ur-Rehman, Mannan A, Inayatullah S, Akhtar MZ, Qayyum M, Mirza B 2009. Biological Evaluation of Wild Thyme (Thymus serpyllum). Pharm Biol 47(7): 628-633.
  • Badawy MEI, Abdelgaleil SAM 2014. Composition and Antimicrobial Activity of Essential Oils Isolated from Egyptian Plants against Plant Pathogenic Bacteria and Fungi. Ind Crops Prod 52: 776-782.
  • Bakkali F, Averbeck S, Averbeck D, Waomar M 2008. Biological Effects of Essential Oils-A Review. Food Chem Toxicol 46: 446.
  • Basim H, Yegen O, Zeller W 2000. Antibacterial Effect of Essential Oil of Thymbra spicata L. var. spicata on Some Plant Pathogenic Bacteria. Journal of Plant Diseases and Protection 107(3): 279-284.
  • Bella P, Catara V, Guarino C, Cirvilleri G 2006. Evaluation of Oleander Accessions for Resistance to Pseudomonas savastanoi pv. nerii. J Plant Pathol 88: 273-278.
  • Ben Hsouna A, Touj N, Hammami I, Dridi K, Al-Ayed AS, Hamdi N 2019. Chemical Composition and In vivo Efficacy of The Essential Oil of Mentha piperita L. in The Suppression of Crown Gall Disease on Tomato Plants. J Oleo Sci 68(5): 419-426.
  • Bliss FA, Almedhi AA, Dandekar AM, Schuerman PL, Bellaloui NI 1999. Crown Gall Resistance in Accessions of Prunus species. HortScience 34: 326-330.
  • Bouchekouk C, Kara FZ, Tail G, Saidi F, Benabdelkader T 2019. Essential Oil Composition and Antibacterial Activity of Pteridium aquilinum (L.) Kuhn. Biologia Futura 70(1): 56-61.
  • Bozkurt İA, Soylu S, Mirik M, Serçe ÇU, Baysal Ö 2014. Characterization of Bacterial Knot Disease Caused by Pseudomonas savastanoi pv savastanoi on Pomegranate Punica granatum L Trees a New Host of the Pathogen. Lett Apllied Microbiol 59: 520-527.
  • Burt S 2004. Essential Oils: Their Antibacterial Properties and Potential Applications in Foods-A Review. Int J Food Microbiol 94: 223.
  • Caballo-Ponce E, Murillo J, Martínez-Gil M, Moreno-Pérez A, Pintado A, Ramos C 2017. Knots Untie: Molecular Determinants Involved in Knot Formation Induced by Pseudomonas savastanoi in Woody Hosts. Front Plant Sci 8: 1089.
  • Churklam W, Chaturongakul S, Ngamwongsatit B, Aunpad R 2020. The Mechanisms of Action of Carvacrol and Its Synergism with Nisin against Listeria monocytogenes on Sliced Bologna Sausage. Food Control 108.
  • da Silva RS, de Oliveira MMG, de Melo JO, Blank AF, Correa CB, Scher R, Fernandes RPM 2019. Antimicrobial Activity of Lippia gracilis Essential Oils on The Plant Pathogen Xanthomonas campestris pv. campestris and Their Effect on Membrane Integrity. Pestic Biochem Phys 160: 40-48.
  • Duman K, Soylu S 2019. Characterization of Antagonistic and Plant Growth-Promoting Traits of Endophytic Bacteria Isolated from Bean Plants against Pseudomonas syringae pv. phaseolicola. Plant Protection Bulletin 59(3): 59-69.
  • Elshafie HS, Sakr S, Mang SM, Belviso S, De Feo V, Camele I 2016. Antimicrobial Activity and Chemical Composition of Three Essential Oils Extracted from Mediterranean Aromatic Plants. J Med Food 19(11): 1096-1103.
  • Godena S, Dminić İ, Đermić E 2012. Differential Susceptibility of Olive Varieties to Olive Knot Disease in Istria. J Cent Eur Agric 13(1): 85-94. Iacobellis NS, Lo Cantore P, Capasso F, Senatore F 2005. Antibacterial Activity of Cuminum cyminum L. and Carum carvi L. Essential Oils. J Agric Food Chem 53(1): 57-61.
  • Jovanka L, Ivana C, Goran T, Sava P, Slavica S, Tamara CG, Ljiljana K 2011. In vitro Antibacterial Activity of Essential Oils from Plant Family Lamiaceae. Rom Biotech Lett 16(2): 6034-6041.
  • Kachur K, Suntres Z 2019. The Antibacterial Properties of Phenolic Isomers, Carvacrol and Thymol. Crit Rev Food Sci Nutr doi:10.1080/10408398.2019.1675585
  • Kaya K, Sertkaya E, Üremiş İ, Soylu S 2018. Determination of Chemical Composition and Fumigant Insecticidal Activities of Essential Oils of Some Medicinal Plants Against the Adults of Cowpea Weevil, Callosobruchus maculatus. KSU J. Agric. Nat. 21:708-714.
  • Kordali S, Cakir A, Ozer H, Cakmakci R, Kesdek M, Mete E 2008. Antifungal, Phytotoxic and Insecticidal Properties of Essential Oil Isolated From Turkish Origanum acutidens and Its Three Components, Carvacrol, Thymol and p-Cymene. Bioresource Technology 99: 8788–8795.
  • Li Q, Guo R, Li Y, Hartman WH, Li S, Zhang Z, Tringe SG, Wang H 2019. Insight into The Bacterial Endophytic Communities of Peach Cultivars Related to Crown Gall Disease Resistance. Appl Environ Microbiol 85: e02931-18.
  • Liu QC, Qiao K, Zhang SA 2019. Potential of A Small Molecule Carvacrol in Management of Vegetable Diseases. Molecules 24(10): 1932.
  • Lucas GC, Alves E, Pereira RB, Perina FJ, de Souza RM 2012. Antibacterial Activity of Essential Oils on Xanthomonas vesicatoria and Control of Bacterial Spot in Tomato. Pesqui Agropecu Bras 47(39): 351-359.
  • Mamadalieva NZ, Akramov DK, Ovidi E, Tiezzi A, Nahar L, Azimova SS, Sarker SD 2017. Aromatic Medicinal Plants of the Lamiaceae family from Uzbekistan: Ethnopharmacology, Essential Oils Composition, and Biological Activities. Medicines (Basel) 4(1): 8.
  • Mansfield J, Genin S, Magori S, Citovsky V, Sriariyanum M, Ronald P, Dow M, Verdier V, Beer SV, Machado MA, Toth I, Salmond G, Foster GD 2012. Top 10 Plant Pathogenic Bacteria in Molecular Plant Pathology. Mol Plant Pathol 13(6): 614–629.
  • Melkani AB, Mohan L, Pant CC, Negi A, Dev V 2011. Terpenoid Composition and Antibacterial Activity of Essential Oil from Salvia hians Royle ex. Benth. J Essent Oil Bear Pl 14(6): 667-672.
  • Mengulluoglu M, Soylu S 2012. Antibacterial Activities of Essential Oils from Several Medicinal Plants against The Seed-Borne Bacterial Disease Agent Acidovorax avenae subsp. citrulli. Res Crop 13: 641-646.
  • Mirik M, Aysan Y 2011. Marmara Bölgesinde Zeytin Dal Kanseri Hastalığının Yaygınlığı ve Pseudomonas savastanoi pv savastanoi Izolatlarının Fenotipik Ve Genotipik Karekterizasyonu. J Agric Sci 17: 279-290.
  • Mirik M, Aysan Y, Şahin F 2011. Characterization of Pseudomonas savastanoi pv savastanoi Strains Isolated From Several Host Plants in Turkey and Report of Fontanesia as a New Host. J Plant Pathol 93: 263-270.
  • Moghaddam M, Alymanesh, MR, Mehdizadeh L, Mirzaei H, Pirbalouti AG 2014. Chemical Composition and Antibacterial Activity of Essential Oil of Ocimum ciliatum, as A New Source of Methyl Chavicol, against Ten Phytopathogens. Ind Crops Prod 59: 144-148.
  • Mohan L, Negi A, Melkani AB, Dev V 2011. Chemical Composition and Antibacterial Activity of Essential Oil from Salvia mukerjeei. Nat Prod Commun 6(12): 1949-1952.
  • Nguyen KA, Förster H, Adaskaveg JE 2018. Efficacy of Copper and New Bactericides for Managing Olive Knot in California. Plant Dis 102: 892-898.
  • Nieto G 2017. Biological Activities of Three Essential Oils of the Lamiaceae Family. Medicines (Basel) 4(3): E63.
  • Öksel C, Mirik M 2015. Zeytin Dal Kanseri Etmeni Pseudomonas savastanoi pv savastanoi’ye Karşı in vitro Koşullarda Farklı Bitkilerin Uçucu Yağlarının Etkisi. Bitki Koruma Bülteni 55: 265-275.
  • Pannek J, Gach J, Boratynski F, Olejniczak T 2018. Antimicrobial Activity of Extracts and Phthalides Occurring in Apiaceae Plants. Phytother Res 32(8): 1459–87.
  • Pulawska J 2010. Crown Gall of Stone Fruits and Nuts, Economic Significance and Diversity of Its Causal Agents: Tumorigenic Agrobacterium spp. J Plant Pathol 92: 87-98.
  • Ramos C, Matas İM, Bardaji L, Aragón İM, Murillo J 2012. Pseudomonas savastanoi pv. savastanoi: Some Like It Knot. Mol Plant Pathol 13(9): 998-1009.
  • Rhouma A, Bouri M, Boubaker A, Nesme X 2008. Potential Effect of Rhizobacteria in The Management of Crown Gall Disease Caused by Agrobacterium tumefaciens Biovar 1. J Plant Pathol 90: 517-526.
  • Rohwer J 1993. Lauraceae. Kubitzki K, Rohwer JG, Bittrich V (eds). The Families and Genera of Vascular Plants Springer:Berlin 2: 366–391.
  • Schollenberger M, Staniek TM, Paduch-Cichal E, Dasiewicz B, Gadomska-Gajadhur A, Mirzwa-Mroz E 2018. The Activity of Essential Oils Obtained from Species and Interspecies Hybrids of the Mentha Genus against Selected Plant Pathogenic Bacteria. Acta Sci Pol-Hortoru 17(6): 167-174.
  • Soylu S, Evrendilek GA, Soylu EM 2009. Chemical Compositions and Antibacterial Activities of Bitter Fennel (Foeniculum vulgare Mill. var. vulgare) and Dill (Anethum graveolens L.) Essential Oils against the Growth of Food-Borne and Seed-Borne Plant Pathogenic Bacteria. Ital J Food Sci 21: 347-355.
  • Turker AU, Yildirim AB, Karakas FP, Turker H 2018. In vitro Antibacterial and Antitumor Efficiency of Some Traditional Plants from Turkey. Indian J Tradit Know 17(1): 50-58.
  • Yong AL, Ooh KF, Ong HC, Chai TT, Wong FC 2015. Investigation of Antibacterial Mechanism and Identification of Bacterial Protein Targets Mediated by Antibacterial Medicinal Plant Extracts. Food Chem 186: 32-36.
  • Young JM 2004. Olive Knot and Its Pathogens. Australasian Plant Pathol 33: 33–39
Primary Language en
Subjects Agriculture
Journal Section RESEARCH ARTICLE
Authors

Orcid: 0000-0002-4826-0317
Author: İ̇mam Adem BOZKURT
Institution: HATAY MUSTAFA KEMAL ÜNİVERSİTESİ
Country: Turkey


Orcid: 0000-0003-1002-8958
Author: Soner SOYLU (Primary Author)
Institution: Hatay Mustafa Kemal Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümü
Country: Turkey


Orcid: 0000-0001-7320-3376
Author: Merve KARA
Institution: HATAY MUSTAFA KEMAL ÜNİVERSİTESİ
Country: Turkey


Orcid: 0000-0001-5961-0848
Author: Emine Mine SOYLU
Institution: HATAY MUSTAFA KEMAL ÜNİVERSİTESİ
Country: Turkey


Thanks This article was presented as a poster abstract in the 1st International Gap Agriculture and Livestock Congress (UGAP 2018, 25/27 April 2018, Şanlıurfa, Turkey).
Dates

Application Date : April 20, 2020
Acceptance Date : June 4, 2020
Publication Date : December 31, 2020

APA Bozkurt, İ , Soylu, S , Kara, M , Soylu, E . (2020). Chemical Composition and Antibacterial Activity of Essential Oils Isolated from Medicinal Plants against Gall Forming Plant Pathogenic Bacterial Disease Agents . Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 23 (6) , 1474-1482 . DOI: 10.18016/ksutarimdoga.vi.723544