Investigation of the Cytotoxic Effect of Ethyl Pyruvate on Various Cancer Cell Lines

Elif Ayazoglu Demir; Selim Demir; İbrahim Turan

doi:10.18016/ksutarimdoga.vi.707661

TR EN

Etil Piruvatın Çeşitli Kanser Hücre Hatları Üzerindeki Sitotoksik Etkisinin İncelenmesi

Öz

Etil piruvat (EP), endojen bir metabolit olan pirüvik asitten türetilen basit bir alifatik esterdir. Çeşitli çalışmalar EP'nin antioksidan ve anti-inflamatuar özelliklerini ortaya koymuş olmasına rağmen, EP'nin kanser hücreleri üzerindeki sitotoksik etkisi hakkında sınırlı sayıda araştırma bulunmaktadır. Bu çalışmanın amacı, EP'nin yaygın kanser türlerini temsil eden hücreler üzerindeki sitotoksik etkilerini belirlemektir. Çalışmada kullanılan EP ticari olarak satın alınmış ve ara stok çözeltiler fosfat tamponu ile hazırlanmıştır. EP'nin insan melanoma (VMM917), serviks (HeLa), meme (MCF-7), akciğer (A549), karaciğer (HepG2), kolon (WiDr) kanseri ve normal fibroblast (BJ) hücreleri üzerindeki sitotoksik etkisi MTT testi kullanılarak belirlenmiştir. Sitotoksisite deneylerinde pozitif kontrol olarak sisplatin kullanılmıştır. Sonuçlar EP'nin BJ hücrelerine kıyasla VMM917 (10.1 kat) ve HeLa (3.04 kat) hücreleri üzerinde seçici sitotoksik etki gösterdiğini ortaya koymuştur. Bu çalışma, EP’nin özellikle melanoma ve serviks kanseri hücreleri üzerinde oldukça seçici bir sitotoksik etkiye sahip olduğunu ilk kez göstermektedir. Bu etkinin mekanizmasının daha kapsamlı çalışmalarla açıklanması gerekmektedir.

Anahtar Kelimeler

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Elif Ayazoglu Demir
0000-0001-9027-7633
Türkiye

Selim Demir ^*
0000-0002-1863-6280
Türkiye

İbrahim Turan
0000-0003-3400-5494
Türkiye

Publication Date

February 28, 2021

Submission Date

March 23, 2020

Acceptance Date

June 1, 2020

Published in Issue

Year 1970 Volume: 24 Number: 1

DOI

https://doi.org/10.18016/ksutarimdoga.vi.707661

IZ

https://izlik.org/JA23YU98DC

Cite

RIS / Bibtex

APA

Ayazoglu Demir, E., Demir, S., & Turan, İ. (2021). Investigation of the Cytotoxic Effect of Ethyl Pyruvate on Various Cancer Cell Lines. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 24(1), 49-56. https://doi.org/10.18016/ksutarimdoga.vi.707661

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Investigation of the Cytotoxic Effect of Ethyl Pyruvate on Various Cancer Cell Lines

Etil Piruvatın Çeşitli Kanser Hücre Hatları Üzerindeki Sitotoksik Etkisinin İncelenmesi

Öz

Anahtar Kelimeler