Investigation of the Cytotoxic Effect of Ethyl Pyruvate on Various Cancer Cell Lines

Elif Ayazoglu Demir; Selim Demir; İbrahim Turan

doi:10.18016/ksutarimdoga.vi.707661

TR EN

Etil Piruvatın Çeşitli Kanser Hücre Hatları Üzerindeki Sitotoksik Etkisinin İncelenmesi

Öz

Etil piruvat (EP), endojen bir metabolit olan pirüvik asitten türetilen basit bir alifatik esterdir. Çeşitli çalışmalar EP'nin antioksidan ve anti-inflamatuar özelliklerini ortaya koymuş olmasına rağmen, EP'nin kanser hücreleri üzerindeki sitotoksik etkisi hakkında sınırlı sayıda araştırma bulunmaktadır. Bu çalışmanın amacı, EP'nin yaygın kanser türlerini temsil eden hücreler üzerindeki sitotoksik etkilerini belirlemektir. Çalışmada kullanılan EP ticari olarak satın alınmış ve ara stok çözeltiler fosfat tamponu ile hazırlanmıştır. EP'nin insan melanoma (VMM917), serviks (HeLa), meme (MCF-7), akciğer (A549), karaciğer (HepG2), kolon (WiDr) kanseri ve normal fibroblast (BJ) hücreleri üzerindeki sitotoksik etkisi MTT testi kullanılarak belirlenmiştir. Sitotoksisite deneylerinde pozitif kontrol olarak sisplatin kullanılmıştır. Sonuçlar EP'nin BJ hücrelerine kıyasla VMM917 (10.1 kat) ve HeLa (3.04 kat) hücreleri üzerinde seçici sitotoksik etki gösterdiğini ortaya koymuştur. Bu çalışma, EP’nin özellikle melanoma ve serviks kanseri hücreleri üzerinde oldukça seçici bir sitotoksik etkiye sahip olduğunu ilk kez göstermektedir. Bu etkinin mekanizmasının daha kapsamlı çalışmalarla açıklanması gerekmektedir.

Anahtar Kelimeler

Investigation of the Cytotoxic Effect of Ethyl Pyruvate on Various Cancer Cell Lines

Abstract

Ethyl pyruvate (EP) is a simple aliphatic ester derived from pyruvic acid which is an endogenous metabolite. Although various studies have investigated the antioxidant and anti-inflammatory properties of EP, there has been only limited research into the cytotoxic effect of EP on cancer cells. The aim of this study was to determine the cytotoxic effects of EP on cells representing common cancer types. EP was purchased commercially and intermediate stock solutions were prepared with phosphate buffer saline. The cytotoxic effect of EP on human melanoma (VMM917), cervix (HeLa), breast (MCF-7), lung (A549), liver (HepG2), colon (WiDr) cancer and normal fibroblast (BJ) cells was determined using the MTT assay. Cisplatin was used as a positive control in cytotoxicity experiments. The results showed that EP exhibits selective cytotoxic effect on VMM917 (10.1-fold) and HeLa (3.04-fold) cells compared to BJ cells. This study shows for the first time that EP has a highly selective cytotoxic effect, especially on melanoma and cervix cancer cells. The mechanism of this effect needs to be elucidated by more extensive studies.

Keywords

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Yapısal Biyoloji

Bölüm

Araştırma Makalesi

Yazarlar

Elif Ayazoglu Demir
0000-0001-9027-7633
Türkiye

Selim Demir ^*
0000-0002-1863-6280
Türkiye

İbrahim Turan
0000-0003-3400-5494
Türkiye

Yayımlanma Tarihi

28 Şubat 2021

Gönderilme Tarihi

23 Mart 2020

Kabul Tarihi

1 Haziran 2020

Yayımlandığı Sayı

Yıl 2021 Cilt: 24 Sayı: 1

DOI

https://doi.org/10.18016/ksutarimdoga.vi.707661

IZ

https://izlik.org/JA23YU98DC

Kaynak Göster

RIS / Bibtex

APA

Ayazoglu Demir, E., Demir, S., & Turan, İ. (2021). Investigation of the Cytotoxic Effect of Ethyl Pyruvate on Various Cancer Cell Lines. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 24(1), 49-56. https://doi.org/10.18016/ksutarimdoga.vi.707661