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Pre ve Post Embriyonik Dönemdeki Civcivlerin Kalp Ventrikülleri ve Purkinje Hücrelerine Histokimyasal Bir Yaklaşım

Year 2021, Volume: 24 Issue: 3, 641 - 649, 30.06.2021

Gamze Turgay İzzetoğlu İnci Turan Özgan Servet Yalçın

https://doi.org/10.18016/ksutarimdoga.vi.763059

Abstract

Bu çalışmada embriyo ve civcivlerde kalp ventrikül duvarı, iletim sistemi ve Purkinje hücreleri üzerine yumurta ağırlığı ve damızlıkların gelişim hızının etkisinin araştırılması amaçlanmıştır. Bu amaçla hızlı gelişen Ross 308 ve yavaş gelişen Hubbard JA genotipindeki damızlık sürülerinden elde edilen tavuk yumurtaları hafif (64±1g) ve ağır (72±1g) olarak iki gruba ayrılmıştır. Kuluçkanın 18. ve 21. günlerinde alınan kalp ventriküllerinin boyuna kesitlerine farklı histokimyasal boyalar uygulanarak, ventrikül duvarı, iletim sistemi ve Purkinje hücreleri incelenmiştir. Genotipin gelişim hızı ve yumurta ağırlığı, kalp ventriküllerinde histoloji açısından herhangi bir farklılığa yol açmamıştır. Ventrikül duvarlarında miyofibriller arası boşluk oluşumu ve kollajen-miyofibril yoğunluk farklılığı görülmüş, bu durumun embriyonik gelişimden kaynaklandığı düşünülmüştür. Histolojik bir çalışmada farklı histokimyasal boyaların kullanılması, organizmaların embriyonik gelişimlerinin takibinde farklı yapıların incelenmesine olanak sağlamaktadır.

Keywords

Civciv kalbi Ventrikül duvarı Purkinje hücreleri Histokimya

Supporting Institution

TÜBİTAK

Project Number

115O434

Thanks

Örneklemeler için Tübitak 115O434 no'lu projeye teşekkür ediyoruz.

References

  • Browder LW, Erickson CA, Jeffery WR 1991. Developmental Biology, 3rd. edition, Philadelphia: Saunders College Publishing, ISBN 0-03-013514-1, 754 pp.
  • Davies F 1930. The Conducting System of the Bird's Heart, J Anat. 64(Pt 2): 129-146.
  • De Jong, F, Opthof T, Wilde AA, Janse MJ, Charles R, Lamers WH, Moorman AF 1992. Persisting Zones of Slow Impulse Conduction in Developing Chicken Hearts. Circ Res 71(2): 240–250.
  • Dzialowski EM, Crossley DA 2015. The Cardiovascular System, Chapter 11, 193-283pp, Sturkie's Avian Physiology, 6th edition, Ed. Scanes CG, Elsevier Inc., ISBN 978-0-12-407160-5, 1056 pp.
  • Fitzgerald BC, Beaufrère H 2016. Cardiology, Chapter 6, 252-328pp, Current Therapy in Avian Medicine and Surgery, 1st edition, Ed. Speer BL, Elsevier Inc., ISBN 978-1-4557-4671-2, 928 pp.
  • Gourdie RG, Wei Y, Kim D, Klatt SC, Mikawa T 1998. Endothelin-Induced Conversion of Embryonic Heart Muscle Cells into Impulse-Conducting Purkinje Fibers. PNAS 95(12): 6815-6818.
  • Gourdie RG, Harris BS, Bond J, Justus C, Hewett KW, O’Brien TX., Thompson RP, Sedmera D 2003. Development of the Cardiac Pacemaking and Conduction System. Birth Defects Research (Part C) 69: 46–57.
  • Groot ACG-d, Winter EM, Poelmann RE 2010. Epicardium-Derived Cells (EPDCs) in Development, Cardiac Disease and Repair of Ischemia. J Cell Mol Med 14(5): 1056-1060.
  • Hamburger V, Hamilton HL 1951. A Series of Normal Stages in The Development of The Chick Embryo. J Morphol 88(1): 49-92.
  • Humason GL 1962. Animal Tissue Techniques. WH Freeman and Company, San Francisco, 61-17383, 468 pp. Ideker RE, Kong W, Pogwizd S 2009. Purkinje Fibers and Arrhythmias. Pacing Clin Electrophysiol 32(3): 283-285.
  • James TN 1964. Anatomy of the A-V node of the dog. The Anatomical Record. 148: 15-27.
  • Lu Y, James TN, Bootsma M, Terasaki F 1993a. Histological Organization of The Right and Left Atrioventricular Valves of The Chicken Heart and Their Relationship to The Atrioventricular Purkinje Ring and The Middle Bundle Branch. Anat Rec 235(1): 74-86.
  • Lu Y, James TN, Yamamoto S, Terasaki F 1993b. Cardiac Conduction System in The Chicken: Gross Anatomy Plus Light and Electron Microscopy. Anat Rec 236(3): 493-510.
  • Martinsen BJ 2005. Reference Guide to the Stages of Chick Heart Embryology. Developmental Dynamics 233: 1217–1237.
  • Mikawa T, Hurtado R 2007. Development of the Cardiac Conduction System, Seminars in Cell & Developmental Biology 18: 90–100.
  • Öber A 2009. Zoolojide Laboratuvar Teknikleri. 3. baskı. Ege Üniversitesi Basımevi, İzmir, ISBN: 978-975-483-824-4, 209 sy.
  • Özfiliz N, Erdost H, Zık B 2010. Veteriner Embriyoloji, 4. Baskı, Ed. Özer A, Nobel Yayın Dağıtım, Ankara, ISBN: 978-9944-77-205-1, 272 sy.
  • Presnell JK, Schreibman MP 1997. Humason’s Animal Tissue Tecniques. 5th edition. The Johns Hopkins University Press Ltd, London ISBN: 0-8018-5401, 572 pp.
  • Sarıca M, Yamak US 2010. Yavaş Gelişen Etlik Piliçlerin Özellikleri ve Geliştirilmesi, Anadolu Tarım Bilim. Derg., 2010,25(1): 61-67.
  • Sarıca M, Ceyhan V, Yamak US, Uçar A, Boz MA 2016. Yavaş Gelişen Sentetik Etlik Piliç Genotipleri ile Ticari Etlik Piliçlerin Büyüme, Karkas Özellikleri ve Bazı Ekonomik Parametreler Bakımından Karşılaştırılması, Tarım Bilimleri Dergisi – Journal of Agricultural Sciences, 22: 20-31.
  • Sedmera D, Reckova M, Bigelow MR, Dealmeida A, Stanley CP, Mikawa T, Gourdie RG, Thompson RP 2004. Developmental Transitions in Electrical Activation Patterns in Chick Embryonic Heart. The Anatomical Record 280A(2): 1001–1009.
  • Strunk A, Wilson GH 2003. Avian cardiology. Vet Clin Exot Anim 6: 1–28.
  • Swiderski RE, Daniels KJ, Jensen KL, Solursh M 1994. Type II Collagen is Transiently Expressed During Avian Cardiac Valve Morphogenesis. Dev Dyn 200(4): 294-304.
  • Tabakoğlu-Oğuz A 2001. Hayvan Embriyolojisi, 2. Baskı, İstanbul Üniversitesi Fen Fakültesi, İstanbul, ISBN: 9754041571, 266 sy.
  • Türkmenoğlu İ, Çevik-Demirkan A, Akosman MS, Akalan MA, Özdemir V 2017. Manda Kalbindeki Sinir Düğümlerinin Makroanatomik, Subgross ve Stereolojik İncelenmesi, Kocatepe Vet J, 10(4): 241-246.
  • Uçar A, Türkoğlu M, Sarıca M 2018. Etlik Piliç ve Ebeveynlerinin Gelişimi, Türk Tarım – Gıda Bilim ve Teknoloji Dergisi, 6(1): 73-77.
  • Ünal G 2018. Hayvan Embriyolojisi, 3. baskı, Nobel Yayın Dağıtım, Ankara, ISBN: 978-605-320-859-4, 136 sy.
  • van Eif VWW, Devalla HD, Boink GJJ, Christoffels VM 2018. Transcriptional Regulation of The Cardiac Conduction System, Cardiac Development doi: 10.1038/s41569-018-0031-y
  • Wesley J 2008. Embryology: Life in Twenty-one Days. https://digitalcommons.usu.edu/cgi/viewcontent.cgi?article=1002&context=extension_curall
  • Wittig JG, Münsterberg A 2016. The Early Stages of Heart Development: Insights from Chicken Embryos. J Cardiovasc Dev Dis 3(12): 1-15.
  • Wittig JG, Münsterberg A 2019. The Chicken as a Model Organism to Study. Cold Spring Harb Perspect Biol doi: 10.1101/cshperspect.a037218
  • Yalçın S, Turgay-İzzetoğlu G., Aktaş A, 2013. Effects of Breeder Age and Egg Weight on Morphological Changes in The Small Intestine of Chicks During the Hatch Window, British Poultry Science, 54(6): 810–817.
  • Yıldırım İ 2005. Broyler Kuluçkalık Yumurta Ağırlığı ve Ebeveyn Sürü Yaşının Embriyo Gelişimi ve Kuluçka Sonuçlarına Etkileri, S.Ü. Ziraat Fakültesi Dergisi 19 (37): 87-91

A Histochemical Approach to Cardiac Ventricles and Purkinje Cells of Chicks in Pre and Post Embryonic Periods

Year 2021, Volume: 24 Issue: 3, 641 - 649, 30.06.2021

Gamze Turgay İzzetoğlu İnci Turan Özgan Servet Yalçın

https://doi.org/10.18016/ksutarimdoga.vi.763059

Abstract

The present study aimed to investigate the effect of egg weight and growth rate of breeders on heart ventricle wall, conduction system and Purkinje cells in embryos and chicks. For this purpose, chicken eggs obtained from fast-growing Ross 308 and slow-growing Hubbard JA broiler breeders were divided into two groups as light (64±1g) and heavy (72±1g) eggs. The ventricle wall, conduction system and Purkinje cells were examined by applying different histochemical dyes to the longitudinal sections of the heart ventricles taken on the 18th and 21st days of the incubation. Genotype growth rate and egg weight did not cause any difference in the histology of the heart ventricles. The formation of inter-myofibrillar space and collagen-myofibril density difference were observed in the ventricular walls, which was thought to be due to the embryonic development. The use of different histochemical dyes in a histological study allows the examination of different structures following the embryonic development of organisms.

Keywords

Chick heart Ventricular wall Purkinje cells Histochemistry

Project Number

115O434

References

  • Browder LW, Erickson CA, Jeffery WR 1991. Developmental Biology, 3rd. edition, Philadelphia: Saunders College Publishing, ISBN 0-03-013514-1, 754 pp.
  • Davies F 1930. The Conducting System of the Bird's Heart, J Anat. 64(Pt 2): 129-146.
  • De Jong, F, Opthof T, Wilde AA, Janse MJ, Charles R, Lamers WH, Moorman AF 1992. Persisting Zones of Slow Impulse Conduction in Developing Chicken Hearts. Circ Res 71(2): 240–250.
  • Dzialowski EM, Crossley DA 2015. The Cardiovascular System, Chapter 11, 193-283pp, Sturkie's Avian Physiology, 6th edition, Ed. Scanes CG, Elsevier Inc., ISBN 978-0-12-407160-5, 1056 pp.
  • Fitzgerald BC, Beaufrère H 2016. Cardiology, Chapter 6, 252-328pp, Current Therapy in Avian Medicine and Surgery, 1st edition, Ed. Speer BL, Elsevier Inc., ISBN 978-1-4557-4671-2, 928 pp.
  • Gourdie RG, Wei Y, Kim D, Klatt SC, Mikawa T 1998. Endothelin-Induced Conversion of Embryonic Heart Muscle Cells into Impulse-Conducting Purkinje Fibers. PNAS 95(12): 6815-6818.
  • Gourdie RG, Harris BS, Bond J, Justus C, Hewett KW, O’Brien TX., Thompson RP, Sedmera D 2003. Development of the Cardiac Pacemaking and Conduction System. Birth Defects Research (Part C) 69: 46–57.
  • Groot ACG-d, Winter EM, Poelmann RE 2010. Epicardium-Derived Cells (EPDCs) in Development, Cardiac Disease and Repair of Ischemia. J Cell Mol Med 14(5): 1056-1060.
  • Hamburger V, Hamilton HL 1951. A Series of Normal Stages in The Development of The Chick Embryo. J Morphol 88(1): 49-92.
  • Humason GL 1962. Animal Tissue Techniques. WH Freeman and Company, San Francisco, 61-17383, 468 pp. Ideker RE, Kong W, Pogwizd S 2009. Purkinje Fibers and Arrhythmias. Pacing Clin Electrophysiol 32(3): 283-285.
  • James TN 1964. Anatomy of the A-V node of the dog. The Anatomical Record. 148: 15-27.
  • Lu Y, James TN, Bootsma M, Terasaki F 1993a. Histological Organization of The Right and Left Atrioventricular Valves of The Chicken Heart and Their Relationship to The Atrioventricular Purkinje Ring and The Middle Bundle Branch. Anat Rec 235(1): 74-86.
  • Lu Y, James TN, Yamamoto S, Terasaki F 1993b. Cardiac Conduction System in The Chicken: Gross Anatomy Plus Light and Electron Microscopy. Anat Rec 236(3): 493-510.
  • Martinsen BJ 2005. Reference Guide to the Stages of Chick Heart Embryology. Developmental Dynamics 233: 1217–1237.
  • Mikawa T, Hurtado R 2007. Development of the Cardiac Conduction System, Seminars in Cell & Developmental Biology 18: 90–100.
  • Öber A 2009. Zoolojide Laboratuvar Teknikleri. 3. baskı. Ege Üniversitesi Basımevi, İzmir, ISBN: 978-975-483-824-4, 209 sy.
  • Özfiliz N, Erdost H, Zık B 2010. Veteriner Embriyoloji, 4. Baskı, Ed. Özer A, Nobel Yayın Dağıtım, Ankara, ISBN: 978-9944-77-205-1, 272 sy.
  • Presnell JK, Schreibman MP 1997. Humason’s Animal Tissue Tecniques. 5th edition. The Johns Hopkins University Press Ltd, London ISBN: 0-8018-5401, 572 pp.
  • Sarıca M, Yamak US 2010. Yavaş Gelişen Etlik Piliçlerin Özellikleri ve Geliştirilmesi, Anadolu Tarım Bilim. Derg., 2010,25(1): 61-67.
  • Sarıca M, Ceyhan V, Yamak US, Uçar A, Boz MA 2016. Yavaş Gelişen Sentetik Etlik Piliç Genotipleri ile Ticari Etlik Piliçlerin Büyüme, Karkas Özellikleri ve Bazı Ekonomik Parametreler Bakımından Karşılaştırılması, Tarım Bilimleri Dergisi – Journal of Agricultural Sciences, 22: 20-31.
  • Sedmera D, Reckova M, Bigelow MR, Dealmeida A, Stanley CP, Mikawa T, Gourdie RG, Thompson RP 2004. Developmental Transitions in Electrical Activation Patterns in Chick Embryonic Heart. The Anatomical Record 280A(2): 1001–1009.
  • Strunk A, Wilson GH 2003. Avian cardiology. Vet Clin Exot Anim 6: 1–28.
  • Swiderski RE, Daniels KJ, Jensen KL, Solursh M 1994. Type II Collagen is Transiently Expressed During Avian Cardiac Valve Morphogenesis. Dev Dyn 200(4): 294-304.
  • Tabakoğlu-Oğuz A 2001. Hayvan Embriyolojisi, 2. Baskı, İstanbul Üniversitesi Fen Fakültesi, İstanbul, ISBN: 9754041571, 266 sy.
  • Türkmenoğlu İ, Çevik-Demirkan A, Akosman MS, Akalan MA, Özdemir V 2017. Manda Kalbindeki Sinir Düğümlerinin Makroanatomik, Subgross ve Stereolojik İncelenmesi, Kocatepe Vet J, 10(4): 241-246.
  • Uçar A, Türkoğlu M, Sarıca M 2018. Etlik Piliç ve Ebeveynlerinin Gelişimi, Türk Tarım – Gıda Bilim ve Teknoloji Dergisi, 6(1): 73-77.
  • Ünal G 2018. Hayvan Embriyolojisi, 3. baskı, Nobel Yayın Dağıtım, Ankara, ISBN: 978-605-320-859-4, 136 sy.
  • van Eif VWW, Devalla HD, Boink GJJ, Christoffels VM 2018. Transcriptional Regulation of The Cardiac Conduction System, Cardiac Development doi: 10.1038/s41569-018-0031-y
  • Wesley J 2008. Embryology: Life in Twenty-one Days. https://digitalcommons.usu.edu/cgi/viewcontent.cgi?article=1002&context=extension_curall
  • Wittig JG, Münsterberg A 2016. The Early Stages of Heart Development: Insights from Chicken Embryos. J Cardiovasc Dev Dis 3(12): 1-15.
  • Wittig JG, Münsterberg A 2019. The Chicken as a Model Organism to Study. Cold Spring Harb Perspect Biol doi: 10.1101/cshperspect.a037218
  • Yalçın S, Turgay-İzzetoğlu G., Aktaş A, 2013. Effects of Breeder Age and Egg Weight on Morphological Changes in The Small Intestine of Chicks During the Hatch Window, British Poultry Science, 54(6): 810–817.
  • Yıldırım İ 2005. Broyler Kuluçkalık Yumurta Ağırlığı ve Ebeveyn Sürü Yaşının Embriyo Gelişimi ve Kuluçka Sonuçlarına Etkileri, S.Ü. Ziraat Fakültesi Dergisi 19 (37): 87-91
There are 33 citations in total.

Details

Primary Language Turkish
Subjects Structural Biology
Journal Section RESEARCH ARTICLE
Authors

Gamze Turgay İzzetoğlu 0000-0001-9828-2402

İnci Turan Özgan 0000-0003-3622-2257

Servet Yalçın 0000-0003-4194-0536

Project Number 115O434
Publication Date June 30, 2021
Submission Date July 7, 2020
Acceptance Date September 15, 2020
Published in Issue Year 2021Volume: 24 Issue: 3

Cite

APA Turgay İzzetoğlu, G., Turan Özgan, İ., & Yalçın, S. (2021). Pre ve Post Embriyonik Dönemdeki Civcivlerin Kalp Ventrikülleri ve Purkinje Hücrelerine Histokimyasal Bir Yaklaşım. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 24(3), 641-649. https://doi.org/10.18016/ksutarimdoga.vi.763059
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