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Artvin Şavşat Yöresi Propolisinin Farklı Sıcaklıklardaki PBS’li Ekstraktlarının Antioksidan Özelliklerinin ve Eritrosit Hemoliz İnhibisyonu Üzerine Etkisinin Araştırılması

Year 2021, Volume: 24 Issue: 3, 464 - 472, 30.06.2021

Deniz Canbolat İbrahim Turan Yunus Emre Küpeli Sedanur Kılınç Sevim Piliç

https://doi.org/10.18016/ksutarimdoga.vi.765838

Abstract

Geleneksel tıpta yüzyıllardır tüm dünyada kullanılan propolis, bal arılarının bitkilerin tomurcuklarını ve polen tanelerini salgıladıkları enzimlerle karıştırıp elde ettikleri kompleks içeriğe sahip reçinemsi doğal bir üründür. Toplanma bölgesi, bitki kaynağı, arı çeşitliliği ve toplandığı yerin ikliminin farklı olması sebepleriyle propolis farklı içeriklere sahip olabilmektedir. Farklı bölgelerle ilgili çok sayıda çalışma olmasına rağmen, Artvin ilinin Şavşat bölgesine ait propolis ile ilgili herhangi bir çalışmaya rastlanılmamıştır. Bu çalışmanın amacı Artvin Şavşat yöresine ait propolisin farklı sıcaklıklarda (45 ve 60 ºC) fosfat tampon tuz çözeltisi (PBS) ile hazırlanan ekstraktlarının antioksidan özelliklerinin (demir indirgeyici güç tayini, 2,2-difenil-1-pikrilhidrazil (DPPH) radikal süpürme aktivitesi, toplam fenolik ve toplam flavonoid madde miktarı) tespit edilmesi ve ekstraktların eritrosit hemoliz inhibisyon özelliklerinin belirlenmesidir. PBS’li ekstraktların toplam fenolik (11.91±0.5-24.14±1.38 mg gallik asit eşdeğeri-g propolis) ve toplam flavonoid (1.79±0.03-2.13±0.07 mg kuersetin eşdeğeri-g propolis) madde miktarı, demir indirgeyici güç tayini (12.80±0.36-27.15±0.12 mg askorbik asit eşdeğeri-g propolis), DPPH (%22.86±0.5-31.24±1.33) ve eritrosit hemoliz inhibisyonu (%55.58±0.57-96.37±0.02) spektrofotometrik yöntemler kullanılarak belirlendi. Deneylerin sonucunda 60 ºC sıcaklıkta elde edilen PBS’li ekstraktın, antioksidan özellikleri ve eritrosit hemolizini inhibe etme oranının 45 ºC de elde edilen ekstrakta göre yüksek olduğu tespit edildi. Ekstraktların eritrosit hemolizini inhibe edici ve yüksek antioksidan özellik gösteren moleküllerinin belirlenmesi için daha ileri çalışmalar gerekmektedir.

Keywords

Antioksidan Aktivite Hemoliz İnhibisyonu Propolis

References

  • Ahn MR, Kumazawa S, Usui Y, Nakamura J, Matsuka M, Zhu F, Nakayama T 2007. Antioxidant Activity and Constituents of Propolis Collected in Various Areas of China. Food Chemistry 101(4): 1383-1392.
  • Aliyazıcıoglu R, Sahin H, Erturk O, Ulusoy E, Kolayli S 2013. Properties of Phenolic Composition and Biological Activity of Propolis from Turkey. International Journal of Food Properties 16(2):277–287.
  • Aliyazicioglu Y, Deger O, Ovali E, Barlak Y, Hosver I, Tekelioglu Y, Karahan SC 2005. Effects of Turkish Pollen and Propolis Extracts on Respiratory Burst for K-562 Cell Lines. International Immunopharmacology 5(11): 1652–1657.
  • Bakkaloğlu Z, Arıcı M 2019. Farklı Çözücülerle Propolis Ekstraksiyonunun Toplam Fenolik İçeriği, Antioksidan Kapasite ve Antimikrobiyal Aktivite Üzerine Etkileri. Akademik Gıda 17(4):538-545.
  • Balkan S 2017. Eritrositlerde İn Vitro Oksidatif Strese Karşı Antioksidan Olarak Değerlendirilen Çeşitli Bitki Ekstraktları. Trakya University Journal of Natural Sciences 18(2): 185-191.
  • Brieger K, Schiavone S, Miller FJJ, Krause KH 2012. Reactive Oxygen Species: From Health To Disease.Swiss Medical Weekly 142:1-14.
  • Bucic-Kojic A, Planinic M, Tomas S, Bilic M, Velic D., 2007. Study of solid–liquid extraction kinetics of total polyphenols from grape seeds. Journal of Food Engineering 81: 236–242.
  • Burdock GA 1998. Review of the Biological Properties and Toxicity of Bee Propolis. Food and Chemical Toxicology 36:347-363.
  • Can Z, Yıldız O, Şahin H, Asadov A, Kolaylı S 2015. Phenolic Profile and Antioxidant Potential Of Propolis From Azerbaijan. Mellifera 15(1):16-28.
  • Çakıroğlu TN 2010. Çeşitli Çözücülerde Türk Propolisinin Çözünürlüğünün İncelenmesi. Karadeniz Teknik Üniversitesi Sağlık Bilimleri Enstitüsü Tıbbi Biyokimya Anabilim Dalı, Yüksek Lisans Tezi, 63 sy.
  • Carl H, Soumya R, Srinivas P, Vani R, 2016. Oxidative stress in erythrocytes of banked ABO blood. Hematology 21(10): 630-634.
  • Da-Silva Frozza CO, Celi Garcia CS, Gambato G, Oliveira de Souza MD, Salvador M, Moura S, Padilha FF, Seixas FK, Collares T, Borsuk S, Dellagostin OA, Henriques JAP, Roesch-Ely M 2013. Chemical Characterization, Antioxidant and Cytotoxic Activities of Brazilian Red Propolis. Food Chem Toxicol 52:137-142.
  • Demir F, Aliyazıcıoğlu Y, Demir D 2017. Asidifikasyon İşleminin Laurocerasus officinalis Ekstraktlarının Antioksidan Özellikleri Üzerine Etkisinin İncelenmesi. El-Cezerî Journal of Science and Engineering 4(3):365-373.
  • Demir S, Aliyazicioglu Y, Turan I, Misir S, Mentese A, Yaman SO, Akbulut K, Kilinc K, Deger O 2016. Antiproliferative and Proapoptotic Activity of Turkish Propolis on Human Lung Cancer Cell Line. Nutrition and Cancer 68(1):165-172.
  • Ertürk Ö, Çil E, Yoloğlu N, Yavuz C 2016. An In vitro Study on Antimicrobial and Antioxidant Activity of Propolis from Rize Province of Turkey. Mellifera 16(1):4–18.
  • Frozza CO, Garcia CS, Gambato G, Souza MD, Salvador M, Moura S, Padilha FF, Seixas FK, Collares T, Borsuk S, Dellagostin OA, Henriques JA, Roesch M 2013. Chemical Characterization, Antioxidant and Cytotoxic Activities of Brazilian Red Propolis. Food Chem Toxicol 52: 137-142.
  • Ighodaro OM, Akinloye OA 2018. First Line Defence Antioxidants-Superoxide Dismutase (SOD), Catalase (CAT) and Glutathione Peroxidase (GPX): Their Fundamental Role in the Entire Antioxidant Defence Grid. Alexandria Journal of Medicine 54: 287–293.
  • Kanbur M, Eraslan G, Silici S 2009. Antioxidant Effect of Propolis Against Exposure to Propetamphos in Rats. Ecotoxicology and Environmental Safety 72(3):909–915.
  • Kozima ET, Souza ABF, Castro TF, Matos NA, Philips NE, Costa GP, Talvani A, Cangussú SD, Bezerra FS 2020. Aluminum Hydroxide Nebulization-İnducedRedox İmbalance and Acute Lung İnflammation in Mice. Experimental Lung Research 21: 1-11.
  • MacDonald-Wicks LK, Wood LG, Garg ML 2006. Methodology for the Determination of Biological Antioxidant Capacityin Vitro: a Review. Journal of the Science of Food and Agriculture 86(13):2046–2056.
  • Meeking MM, MacFabe DF, Mepham JR, Foley KA, Tichenoff LJ, Boon FH, Kavaliers M, Ossenkopp KP 2020. Propionic Acid İnduced Behavioural Effects of Relevance to Autism Spectrum Disorder Evaluated in the Hole Board Test With Rats. Progress in Neuropsychopharmacology and Biological Psychiatry 97:1-13.
  • Meral R 2017. Farklı Sıcaklık Derecelerinin Uşkun Bitkisinin Antioksidan Aktivitesi ve Fenolik Profili Üzerine Etkisi. YYÜ Tar Bil Dergisi27(1): 88-94.
  • Mercan U 2004. Toksikolojide Serbest Radikallerin Önemi. Yüzüncü Yıl ÜniversitesiVeterinerlik Fakültesi Dergisi 15(1-2): 91-96.
  • Mısır S 2013. Türk Propolisinin Farklı Çözücülerdeki Ekstraktlarının Radikal Yakalama Ve Demir Şelatlama Aktivitesinin İncelenmesi. Karadeniz Teknik Üniversitesi Sağlık Bilimleri Enstitüsü Tıbbi Biyokimya Anabilim Dalı, Yüksek Lisans Tezi, 60 sy.
  • Miguel MG, Nunes S, Dandlen SA, Cavaco AM, Antunes MD 2014. Phenols, Flavonoids and Antioxidant Activity of Aqueous and Methanolic Extracts of Propolis (Apis Mellifera L.) from Algarve, South Portugal. Food Sci. Technol, Campinas 34(1): 16-23.
  • Misir S, Aliyazicioglu Y. Demir S, Turan I, Ozer Yaman S, Deger O 2018. Antioxidant Properties and Protective Effect of Turkish Propolis on t-BHP-Induced Oxidative Stress in Foreskin Fibroblast Cells. Indian Journal of Pharmaceutical Education and Research 52:94-100.
  • Moreno MI, Isla MI, Sampietro AR, Vattuone MA 2000. Comparison of the Free Radical-Scavenging Activity of Propolis from Several Regions of Argentina. Journal of Ethnopharmacology 71(1-2): 109-114.
  • Okoko T, Ere D 2012. Reduction of Hydrogen Peroxide-İnduced Erythrocyte Damage by Carica papaya Leaf Extract. Asian Pac J Trop Biomed 2(6): 449-453.
  • Onbaşılı D, Yuvalı Çelik G, Kahraman S, Kanbur M 2019. Apiterapi ve İnsan Sağlığı Üzerine Etkileri. Erciyes Üniv Vet Fak Derg 16(1): 55-62.
  • Oyaizu M 1986. Studies on Product of Browning Reaction Prepared from Glucose Amine. Japanese Journal of Nutrition 44: 307-315.
  • Özcan O, Erdal H, Çakırca G, Yönden Z 2015. Oxidative Stress and İts İmpacts On İntracellular Lipids, Proteins and DNA. Journal of Clinical and Experimental Investigations 6 (3): 331-336.
  • Pala UÇ, Ekşi CN, Özçelik E, Çam AB 2017. Geleneksel Meyan Kökü Şerbeti Hazırlama Sürecinde Farklı Sıcaklık Uygulamalarının Şerbetin Mikrobiyolojik Kalitesi ve Biyoaktif Bileşenleri Üzerine Etkisi. Journal of Tourism and Gastronomy Studies 2:276-286.
  • Pereira AD, De-Andrade SF, De-Oliveira SMS, Maistro EL 2008. First İn Vivo Evaluation of the Mutagenic Effect of Brazilian Green Propolis by Comet Assay and Micronucleus Test. Food and Chemical Toxicology 46(7): 2580–2584.
  • Postaci I, Coskun O, Senol N, Aslankoc R, Comlekci S 2018. The Physiopathological Effects of Quercetin on Oxidative Stress in Radiation of 4.5 g Mobile Phone Exposed Liver Tissue of Rat. Bratisl Lek Listy 119(8):481-489.
  • Ramchoun M, Sellam K, Harnafi H, Alem C, Benlyas M, Khallouki F, Amrani S 2015. Investigation of Antioxidant and Antihemolytic Properties of Thymus Satureioides Collected From Tafilalet Region, South-East of Morocco. Asian Pacific Journal of Tropical Biomedicine 5(2): 93–100.
  • Rufatto LC, Dos Santos DA, Marinho F, Henriques, JAP, Ely MR, Moura S 2017. Red Propolis: Chemical Composition and Pharmacological Activity. Asian Pac. J. Trop. Biomed 7(7): 591–598.
  • Sajid M, Khan MR, Ismail H, Latif S, Rahim AA, Mehboob R, Shah SA 2020Antidiabetic and Antioxidant Potential of Alnus Nitida Leaves in Alloxan İnduced Diabetic Rats. Journal of Ethnopharmacology 1-33
  • Sezer K, Keskin M 2014. Serbest Oksijen Radikallerinin Hastalıkların Patogenezisindeki Rolü. F.Ü.Sağlık Bilimleri Veterinerlik Dergisi 28(1): 49-56.
  • Sforcin JM, Bankova V 2011. Propolis: is There a Potential for the Development of New Drugs?. J Ethnopharmacol 133: 253-260.
  • Shinde G, Patil AS, Sheikh R 2017. Reduction of Hydrogen Peroxide-Induced Erythrocyte Damage by Leaf Extracts of Buchanania Lanzan Spreng as a Potential Natural Antioxidant. Austin J Biotechnol Bioeng 4(2): 1-7.
  • Siripatrawan U, Vitchayakitti W, Sanguandeekul R 2012. Antioxidant and Antimicrobial Properties of Thai Propolis Extracted Using Ethanol Aqueous Solution. Int J Food Scı Tech 48(1):22-7.
  • Slinkard K, Singleton VL 1977. Total Phenol Analysis: Automation and Comparison with Manual Methods. American Journal of Enology and Viticulture 28: 49- 55.
  • Süleyman H, Gül V, Erhan E 2018. Oksidatif Stres ve Doku Hasarı. Erzincan Tıp Dergisi 1(1):1-4
  • Thamnopoulo IAI, Michailidis GF, Fletouris DJ, Badeka A, Kontominas MG, Angelidis AS 2018. Inhibitory Activity of Propolis Against Listeria Monocytogenes in Milk Stored Under Refrigeration. Food Microbiology 73:168–176.
  • Turan I, Demir S, Misir, S., Kilinc K, Mentese A, Aliyazicioglu Y, Deger O 2015. Cytotoxic Effect of Turkish Propolis on Liver, Colon, Breast, Cervix and Prostate Cancer Cell Lines. Tropical Journal of Pharmaceutical Research 14(5): 777-782.
  • Turan İ. Demir S. Aliyazıcıoğlu R. Aliyazıcıoğlu Y 2017. Primula vulgaris Yaprak Ekstraktının Antioksidan ve Sitotoksik Özelliklerinin Değerlendirilmesi. KSÜ Doğa Bil. Derg 20(4):361-367.
  • Yalçın CO, Aliyazicioglu Y, Demir S, Turan I, Bahat Z, Misir S, Deger O 2016. Evaluation of Radioprotective Effect of Turkish Propolis on Foreskin Fibroblast Cells. J Can Res Ther 12(2): 990-994.
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Year 2021, Volume: 24 Issue: 3, 464 - 472, 30.06.2021

Deniz Canbolat İbrahim Turan Yunus Emre Küpeli Sedanur Kılınç Sevim Piliç

https://doi.org/10.18016/ksutarimdoga.vi.765838

Abstract

Propolis, which has been used in traditional medicine for hundreds of years all over the world, is a resinous natural product with a complex content obtained by honey bees by mixing the buds of plants and pollen grains with enzymes they secrete. Propolis may have different contents due to the gathering region, plant source, bee variety and climate of the place where it is collected. Although there are many studies related to different regions, so far the propolis of the Şavşat region in Artvin have not been studied yet. The aim of this study is to determine the antioxidant properties (determination of iron reducing power, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, total phenolic and total flavonoid content) of propolis extracts of Artvin Şavşat region prepared with phosphate buffer salt solution (PBS) prepared at different temperatures (45 and 60 ºC) and to determine the erythrocyte hemolysis inhibition properties of the extracts. Total phenolic (11.91 ± 0.5-24.14 ± 1.38 mg gallic acid equivalent-g propolis) and total flavonoid (1.79 ± 0.03-2.13 ± 0.07 mg quercetin equivalent-g propolis) content of PBS extracts, iron reducing power determination (12.80 ± 0.36-27.15 ± 0.12 mg ascorbic acid equivalent /-g propolis), 2,2-diphenyl-1-picrilhydrazyl (DPPH) radical scavenging activity (%22.86±0.5-31.24±1.33) and erythrocyte hemolysis inhibition (%55.58±0.57-96.37±0.02) were determined using spectrophotometric methods. As a result of the experiments, it was determined that the extract with PBS obtained at a temperature of 60 ºC was higher than the extract obtained at 45 ºC, in terms of antioxidant properties and erythrocyte hemolysis inhibition ratio. Further studies are required to identify molecules that inhibit erythrocyte hemolysis in extracts and have antioxidant properties.

Keywords

Antioxidant Activity Hemolysis Inhibition Propolis

References

  • Ahn MR, Kumazawa S, Usui Y, Nakamura J, Matsuka M, Zhu F, Nakayama T 2007. Antioxidant Activity and Constituents of Propolis Collected in Various Areas of China. Food Chemistry 101(4): 1383-1392.
  • Aliyazıcıoglu R, Sahin H, Erturk O, Ulusoy E, Kolayli S 2013. Properties of Phenolic Composition and Biological Activity of Propolis from Turkey. International Journal of Food Properties 16(2):277–287.
  • Aliyazicioglu Y, Deger O, Ovali E, Barlak Y, Hosver I, Tekelioglu Y, Karahan SC 2005. Effects of Turkish Pollen and Propolis Extracts on Respiratory Burst for K-562 Cell Lines. International Immunopharmacology 5(11): 1652–1657.
  • Bakkaloğlu Z, Arıcı M 2019. Farklı Çözücülerle Propolis Ekstraksiyonunun Toplam Fenolik İçeriği, Antioksidan Kapasite ve Antimikrobiyal Aktivite Üzerine Etkileri. Akademik Gıda 17(4):538-545.
  • Balkan S 2017. Eritrositlerde İn Vitro Oksidatif Strese Karşı Antioksidan Olarak Değerlendirilen Çeşitli Bitki Ekstraktları. Trakya University Journal of Natural Sciences 18(2): 185-191.
  • Brieger K, Schiavone S, Miller FJJ, Krause KH 2012. Reactive Oxygen Species: From Health To Disease.Swiss Medical Weekly 142:1-14.
  • Bucic-Kojic A, Planinic M, Tomas S, Bilic M, Velic D., 2007. Study of solid–liquid extraction kinetics of total polyphenols from grape seeds. Journal of Food Engineering 81: 236–242.
  • Burdock GA 1998. Review of the Biological Properties and Toxicity of Bee Propolis. Food and Chemical Toxicology 36:347-363.
  • Can Z, Yıldız O, Şahin H, Asadov A, Kolaylı S 2015. Phenolic Profile and Antioxidant Potential Of Propolis From Azerbaijan. Mellifera 15(1):16-28.
  • Çakıroğlu TN 2010. Çeşitli Çözücülerde Türk Propolisinin Çözünürlüğünün İncelenmesi. Karadeniz Teknik Üniversitesi Sağlık Bilimleri Enstitüsü Tıbbi Biyokimya Anabilim Dalı, Yüksek Lisans Tezi, 63 sy.
  • Carl H, Soumya R, Srinivas P, Vani R, 2016. Oxidative stress in erythrocytes of banked ABO blood. Hematology 21(10): 630-634.
  • Da-Silva Frozza CO, Celi Garcia CS, Gambato G, Oliveira de Souza MD, Salvador M, Moura S, Padilha FF, Seixas FK, Collares T, Borsuk S, Dellagostin OA, Henriques JAP, Roesch-Ely M 2013. Chemical Characterization, Antioxidant and Cytotoxic Activities of Brazilian Red Propolis. Food Chem Toxicol 52:137-142.
  • Demir F, Aliyazıcıoğlu Y, Demir D 2017. Asidifikasyon İşleminin Laurocerasus officinalis Ekstraktlarının Antioksidan Özellikleri Üzerine Etkisinin İncelenmesi. El-Cezerî Journal of Science and Engineering 4(3):365-373.
  • Demir S, Aliyazicioglu Y, Turan I, Misir S, Mentese A, Yaman SO, Akbulut K, Kilinc K, Deger O 2016. Antiproliferative and Proapoptotic Activity of Turkish Propolis on Human Lung Cancer Cell Line. Nutrition and Cancer 68(1):165-172.
  • Ertürk Ö, Çil E, Yoloğlu N, Yavuz C 2016. An In vitro Study on Antimicrobial and Antioxidant Activity of Propolis from Rize Province of Turkey. Mellifera 16(1):4–18.
  • Frozza CO, Garcia CS, Gambato G, Souza MD, Salvador M, Moura S, Padilha FF, Seixas FK, Collares T, Borsuk S, Dellagostin OA, Henriques JA, Roesch M 2013. Chemical Characterization, Antioxidant and Cytotoxic Activities of Brazilian Red Propolis. Food Chem Toxicol 52: 137-142.
  • Ighodaro OM, Akinloye OA 2018. First Line Defence Antioxidants-Superoxide Dismutase (SOD), Catalase (CAT) and Glutathione Peroxidase (GPX): Their Fundamental Role in the Entire Antioxidant Defence Grid. Alexandria Journal of Medicine 54: 287–293.
  • Kanbur M, Eraslan G, Silici S 2009. Antioxidant Effect of Propolis Against Exposure to Propetamphos in Rats. Ecotoxicology and Environmental Safety 72(3):909–915.
  • Kozima ET, Souza ABF, Castro TF, Matos NA, Philips NE, Costa GP, Talvani A, Cangussú SD, Bezerra FS 2020. Aluminum Hydroxide Nebulization-İnducedRedox İmbalance and Acute Lung İnflammation in Mice. Experimental Lung Research 21: 1-11.
  • MacDonald-Wicks LK, Wood LG, Garg ML 2006. Methodology for the Determination of Biological Antioxidant Capacityin Vitro: a Review. Journal of the Science of Food and Agriculture 86(13):2046–2056.
  • Meeking MM, MacFabe DF, Mepham JR, Foley KA, Tichenoff LJ, Boon FH, Kavaliers M, Ossenkopp KP 2020. Propionic Acid İnduced Behavioural Effects of Relevance to Autism Spectrum Disorder Evaluated in the Hole Board Test With Rats. Progress in Neuropsychopharmacology and Biological Psychiatry 97:1-13.
  • Meral R 2017. Farklı Sıcaklık Derecelerinin Uşkun Bitkisinin Antioksidan Aktivitesi ve Fenolik Profili Üzerine Etkisi. YYÜ Tar Bil Dergisi27(1): 88-94.
  • Mercan U 2004. Toksikolojide Serbest Radikallerin Önemi. Yüzüncü Yıl ÜniversitesiVeterinerlik Fakültesi Dergisi 15(1-2): 91-96.
  • Mısır S 2013. Türk Propolisinin Farklı Çözücülerdeki Ekstraktlarının Radikal Yakalama Ve Demir Şelatlama Aktivitesinin İncelenmesi. Karadeniz Teknik Üniversitesi Sağlık Bilimleri Enstitüsü Tıbbi Biyokimya Anabilim Dalı, Yüksek Lisans Tezi, 60 sy.
  • Miguel MG, Nunes S, Dandlen SA, Cavaco AM, Antunes MD 2014. Phenols, Flavonoids and Antioxidant Activity of Aqueous and Methanolic Extracts of Propolis (Apis Mellifera L.) from Algarve, South Portugal. Food Sci. Technol, Campinas 34(1): 16-23.
  • Misir S, Aliyazicioglu Y. Demir S, Turan I, Ozer Yaman S, Deger O 2018. Antioxidant Properties and Protective Effect of Turkish Propolis on t-BHP-Induced Oxidative Stress in Foreskin Fibroblast Cells. Indian Journal of Pharmaceutical Education and Research 52:94-100.
  • Moreno MI, Isla MI, Sampietro AR, Vattuone MA 2000. Comparison of the Free Radical-Scavenging Activity of Propolis from Several Regions of Argentina. Journal of Ethnopharmacology 71(1-2): 109-114.
  • Okoko T, Ere D 2012. Reduction of Hydrogen Peroxide-İnduced Erythrocyte Damage by Carica papaya Leaf Extract. Asian Pac J Trop Biomed 2(6): 449-453.
  • Onbaşılı D, Yuvalı Çelik G, Kahraman S, Kanbur M 2019. Apiterapi ve İnsan Sağlığı Üzerine Etkileri. Erciyes Üniv Vet Fak Derg 16(1): 55-62.
  • Oyaizu M 1986. Studies on Product of Browning Reaction Prepared from Glucose Amine. Japanese Journal of Nutrition 44: 307-315.
  • Özcan O, Erdal H, Çakırca G, Yönden Z 2015. Oxidative Stress and İts İmpacts On İntracellular Lipids, Proteins and DNA. Journal of Clinical and Experimental Investigations 6 (3): 331-336.
  • Pala UÇ, Ekşi CN, Özçelik E, Çam AB 2017. Geleneksel Meyan Kökü Şerbeti Hazırlama Sürecinde Farklı Sıcaklık Uygulamalarının Şerbetin Mikrobiyolojik Kalitesi ve Biyoaktif Bileşenleri Üzerine Etkisi. Journal of Tourism and Gastronomy Studies 2:276-286.
  • Pereira AD, De-Andrade SF, De-Oliveira SMS, Maistro EL 2008. First İn Vivo Evaluation of the Mutagenic Effect of Brazilian Green Propolis by Comet Assay and Micronucleus Test. Food and Chemical Toxicology 46(7): 2580–2584.
  • Postaci I, Coskun O, Senol N, Aslankoc R, Comlekci S 2018. The Physiopathological Effects of Quercetin on Oxidative Stress in Radiation of 4.5 g Mobile Phone Exposed Liver Tissue of Rat. Bratisl Lek Listy 119(8):481-489.
  • Ramchoun M, Sellam K, Harnafi H, Alem C, Benlyas M, Khallouki F, Amrani S 2015. Investigation of Antioxidant and Antihemolytic Properties of Thymus Satureioides Collected From Tafilalet Region, South-East of Morocco. Asian Pacific Journal of Tropical Biomedicine 5(2): 93–100.
  • Rufatto LC, Dos Santos DA, Marinho F, Henriques, JAP, Ely MR, Moura S 2017. Red Propolis: Chemical Composition and Pharmacological Activity. Asian Pac. J. Trop. Biomed 7(7): 591–598.
  • Sajid M, Khan MR, Ismail H, Latif S, Rahim AA, Mehboob R, Shah SA 2020Antidiabetic and Antioxidant Potential of Alnus Nitida Leaves in Alloxan İnduced Diabetic Rats. Journal of Ethnopharmacology 1-33
  • Sezer K, Keskin M 2014. Serbest Oksijen Radikallerinin Hastalıkların Patogenezisindeki Rolü. F.Ü.Sağlık Bilimleri Veterinerlik Dergisi 28(1): 49-56.
  • Sforcin JM, Bankova V 2011. Propolis: is There a Potential for the Development of New Drugs?. J Ethnopharmacol 133: 253-260.
  • Shinde G, Patil AS, Sheikh R 2017. Reduction of Hydrogen Peroxide-Induced Erythrocyte Damage by Leaf Extracts of Buchanania Lanzan Spreng as a Potential Natural Antioxidant. Austin J Biotechnol Bioeng 4(2): 1-7.
  • Siripatrawan U, Vitchayakitti W, Sanguandeekul R 2012. Antioxidant and Antimicrobial Properties of Thai Propolis Extracted Using Ethanol Aqueous Solution. Int J Food Scı Tech 48(1):22-7.
  • Slinkard K, Singleton VL 1977. Total Phenol Analysis: Automation and Comparison with Manual Methods. American Journal of Enology and Viticulture 28: 49- 55.
  • Süleyman H, Gül V, Erhan E 2018. Oksidatif Stres ve Doku Hasarı. Erzincan Tıp Dergisi 1(1):1-4
  • Thamnopoulo IAI, Michailidis GF, Fletouris DJ, Badeka A, Kontominas MG, Angelidis AS 2018. Inhibitory Activity of Propolis Against Listeria Monocytogenes in Milk Stored Under Refrigeration. Food Microbiology 73:168–176.
  • Turan I, Demir S, Misir, S., Kilinc K, Mentese A, Aliyazicioglu Y, Deger O 2015. Cytotoxic Effect of Turkish Propolis on Liver, Colon, Breast, Cervix and Prostate Cancer Cell Lines. Tropical Journal of Pharmaceutical Research 14(5): 777-782.
  • Turan İ. Demir S. Aliyazıcıoğlu R. Aliyazıcıoğlu Y 2017. Primula vulgaris Yaprak Ekstraktının Antioksidan ve Sitotoksik Özelliklerinin Değerlendirilmesi. KSÜ Doğa Bil. Derg 20(4):361-367.
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There are 48 citations in total.

Details

Primary Language Turkish
Journal Section RESEARCH ARTICLE
Authors

Deniz Canbolat 0000-0003-1328-4762

İbrahim Turan 0000-0003-3400-5494

Yunus Emre Küpeli 0000-0002-3114-941X

Sedanur Kılınç 0000-0002-9093-9550

Sevim Piliç 0000-0003-1217-6188

Publication Date June 30, 2021
Submission Date July 7, 2020
Acceptance Date October 21, 2020
Published in Issue Year 2021Volume: 24 Issue: 3

Cite

APA Canbolat, D., Turan, İ., Küpeli, Y. E., Kılınç, S., et al. (2021). Artvin Şavşat Yöresi Propolisinin Farklı Sıcaklıklardaki PBS’li Ekstraktlarının Antioksidan Özelliklerinin ve Eritrosit Hemoliz İnhibisyonu Üzerine Etkisinin Araştırılması. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 24(3), 464-472. https://doi.org/10.18016/ksutarimdoga.vi.765838
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KSU Journal of Agriculture and Nature

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