Year 2021, Volume 24 , Issue 4, Pages 757 - 765 2021-08-31

Tuz Stresi Altındaki Hıyar Bitkilerinde Ekzojen Askorbik Asit Uygulamalarının Fotosistem II Aktivitesi Üzerindeki Etkileri
Effects of Exogenous Ascorbic Acid Application on Photosystem II Activity in Cucumber Plants under Salt Stress

Sezen TOKSOY [1] , Ali DOĞRU [2]


Tuz stresi (100 mM NaCl) altındaki Beith Alpha hıyar (Cucumis sativus L.) çeşidinde ekzojen askorbik asit uygulamasının fotosistem II aktivitesi üzerindeki etkileri klorofil a floresansı tekniği yardımıyla araştırılmıştır. Tuz stresi hıyar yapraklarında fotosistem II’nin hem donör hem de akseptör bölgesindeki elektron hareketlerini inhibe etmiştir. Ayrıca tuz stresinin hıyar bitkisinde aktif reaksiyon merkezi miktarını ve kinonA ile plastokinonun indirgenme yeteneğini azalttığı, indirgenmiş reaksiyon merkezlerinin birikimini ve termal disipasyon enerjisini artırdığı belirlenmiştir. Askorbik asit uygulaması ise hıyar bitkilerinde tuz stresinin fotosistem II’nin donör ve akseptör bölgesindeki elektron hareketleri üzerindeki olumsuz etkisini ortadan kaldırmıştır. Ek olarak askorbik asit uygulaması hıyar yapraklarındaki aktif reaksiyon merkezi miktarını ve kinonA ile plastokinonun indirgenme yeteneğini artırırken, indirgenmiş reaksiyon merkezi miktarını ve termal disipasyon enerjisini azaltmıştır. Sonuç olarak askorbik asidin hıyar yapraklarında tuz toleransını artırdığı ve bu yaklaşımın tarımsal amaçlarla kullanılabileceği söylenebilir.
The effects of the exogenous ascorbic acid application on photosystem II activity were investigated in salt-stressed (100 mM NaCl) cucumber (Cucumis sativus L.) cv. Beith Alpha through chlorophyll a fluorescence technique. Salt stress inhibited electron movements both in donor and acceptor site of photosystem II in cucumber leaves. In addition, salt application led to the decreased level of active reaction centers, the accumulation of the reduced reaction centers, the decreased ability of quinonA and plastoquinon to reduce and the increased thermal dissipation in cucumber leaves. Ascorbic acid, on the other hand, ameliorated the adverse effect of salt stress on electron movements in donor and acceptor site of photosystem II in cucumber plants. Moreover, ascorbic acid caused to the increased level of active reaction centers, the decreased level of accumulation of the reduced reaction centers, the increased ability of quinonA and plastoquinon to reduce and the decreased thermal dissipation in cucumber leaves. As a result, it might be concluded that ascorbic acid application improved salt tolerance in cucumber plants and it may be used for agricultural purposes.
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Primary Language tr
Subjects Science
Journal Section RESEARCH ARTICLE
Authors

Orcid: 0000-0002-1564-9465
Author: Sezen TOKSOY (Primary Author)
Institution: SAKARYA ÜNİVERSİTESİ
Country: Turkey


Orcid: 0000-0003-0060-4691
Author: Ali DOĞRU
Institution: SAKARYA ÜNİVERSİTESİ
Country: Turkey


Dates

Application Date : May 4, 2020
Acceptance Date : December 17, 2020
Publication Date : August 31, 2021

APA Toksoy, S , Doğru, A . (2021). Tuz Stresi Altındaki Hıyar Bitkilerinde Ekzojen Askorbik Asit Uygulamalarının Fotosistem II Aktivitesi Üzerindeki Etkileri . Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 24 (4) , 757-765 . DOI: 10.18016/ksutarimdoga.vi.732141