Year 2021, Volume 24 , Issue 4, Pages 805 - 814 2021-08-31

Determination of Plum pox virus and Molecular Characterization of Partial Coat Protein Gene in Burdur Province
Burdur İlinde Plum pox virus’un Tespiti ve Kısmi Kılıf Protein Geninin Moleküler Karakterizasyonu

Ali Ferhan MORCA [1] , Sevgi COŞKAN [2] , Ali ÇELIK [3]


Plum pox virus (PPV), the causal agent of Sharka disease, causes significant yield losses in stone fruits. Despite PPV occurrence as restricted in different regions of Turkey, there is no record of PPV yet in Burdur province. Hence, this study includes the results for determining the presence of PPV in Burdur province between 2016-2019 by serological and molecular methods. A total of 47 leaf samples collected during the surveys were first analyzed by Double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), and only 2-peach samples were determined to be infected with PPV. A degenerate primer pair that amplifies a 767 bp product was designed for the partial coat protein region of PPV in this study. Reverse transcription-polymerase chain reaction (RT-PCR) was performed using designed primer pair, and novel isolates obtained by sequence analysis were deposited in the GenBank. As a result of the BlastN analysis, Burdur PPV isolates showed the highest similarity (99.86% -98.49%) with the PPV-M strain. The phylogenetic tree was generated by the Neighbor-Joining method, resulting in novel PPV-Burdur isolates were clustered with the PPV-M isolates belonging to Turkey and other countries. The novel two PPV-M isolates were the first records in the province of Burdur, the PPV-free area in the Mediterranean Region. As a result of this study, by eradicating infected trees, a one-kilometer diameter buffer zone was established. It has been decided to ban the replanting of Prunus species and continue the survey studies for three years.
Plum pox virus (PPV), sert çekirdekli meyvelerde önemli verim kayıplarına sebep olan Şarka hastalığının etmenidir. PPV, Türkiye’nin farklı bölgelerinde sınırlı olarak tespit edilmesine rağmen, bugüne kadar Burdur iline ait herhangi bir kayıt bulunmamaktadır. Bu çalışmada, 2016-2019 yılları arasında Burdur ilinde PPV’nin varlığının belirlenmesine yönelik serolojik ve moleküler yöntemler kullanılarak yapılan araştırmanın sonuçlarına yer verilmiştir. Sürveyler süresince toplanan 47 adet sert çekirdekli meyve yaprak örneği ilk olarak Double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA) ile analiz edilmiş ve 2 adet şeftali örneğinin PPV ile enfekteli olduğu belirlenmiştir. Çalışmada, PPV’nin kılıf protein bölgesi için 767 nükleotid büyüklüğünde bir bölgeyi çoğaltan bir adet dejenere primer çifti tasarlanmıştır. Yeni primerler ile gerçekleştirilen Reverse transcription-polymerase chain reaction (RT-PCR) ve sekans analizleri sonucunda elde edilen diziler GenBank’a kaydedilmiştir. Yapılan BlastN analizi neticesinde Burdur PPV izolatları en yüksek benzerlik oranını (%99.86-%98.49), PPV-M ırkı ile göstermiştir. Neighbour-joining yöntemiyle yapılan filogenetik ağaçta Burdur izolatlarının, Türkiye ve farklı ülkelere ait PPV-M izolatı ile kümelendiği belirlenmiştir. Bu çalışma ile elde edilen 2 adet PPV-M izolatı Akdeniz Bölgesi’nin PPV açısından ari alanı konumunda olan Burdur ilinde ilk kayıt niteliğindedir. Çalışma sonucunda enfekteli olduğu tespit edilen ağaçlar eradike edilerek 1 km çapında tampon bölge oluşturulmuş ve 3 yıl boyunca Prunus türlerinin yetiştiriciliğinin yasaklanmasına ve sürvey çalışmalarınnın devam edilmesine karar verilmiştir.
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Primary Language tr
Subjects Agriculture
Journal Section RESEARCH ARTICLE
Authors

Orcid: 0000-0002-7480-922X
Author: Ali Ferhan MORCA (Primary Author)
Institution: ANKARA ZİRAİ MÜCADELE MERKEZ ARAŞTIRMA ENSTİTÜSÜ MÜDÜRLÜĞÜ
Country: Turkey


Orcid: 0000-0002-3589-6041
Author: Sevgi COŞKAN
Institution: ANKARA ZİRAİ MÜCADELE MERKEZ ARAŞTIRMA ENSTİTÜSÜ MÜDÜRLÜĞÜ
Country: Turkey


Orcid: 0000-0002-5836-8030
Author: Ali ÇELIK
Institution: BOLU ABANT İZZET BAYSAL ÜNİVERSİTESİ, ZİRAAT VE DOĞA BİLİMLERİ FAKÜLTESİ, BİTKİ KORUMA BÖLÜMÜ, BİTKİ KORUMA PR.
Country: Turkey


Supporting Institution Tarım ve Orman Bakanlığı
Thanks Bu çalışmada sürvey çalışmalarında emeği geçen Tarım ve Orman Bakanlığı Burdur İl Tarım ve Orman Müdürlüğünde görevli personelleri ile Tarımsal Araştırmalar ve Politikalar Genel Müdürlüğü Zirai Mücadele Merkez Araştırma Enstitüsü Müdürlüğüne desteklerinden dolayı teşekkür ediyoruz.
Dates

Application Date : July 10, 2020
Acceptance Date : December 4, 2020
Publication Date : August 31, 2021

APA Morca, A , Coşkan, S , Çelık, A . (2021). Burdur İlinde Plum pox virus’un Tespiti ve Kısmi Kılıf Protein Geninin Moleküler Karakterizasyonu . Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 24 (4) , 805-814 . DOI: 10.18016/ksutarimdoga.vi.767409