Year 2021, Volume 24 , Issue 4, Pages 707 - 714 2021-08-31

Partial Characterization of Hydrolytic Enzymes Produced by Bacillus Strains Isolated from Balıklıgöl, Turkey
Balıklıgöl, Türkiye’den İzole Edilen Bacillus Suşları Tarafından Üretilen Hidrolitik Enzimlerin Kısmi Karakterizasyonu

Ebru UYAR [1] , Cengiz ÇORBACI [2]


In the present study, forty-five bacterial isolates were obtained from previously unstudied soil samples in Balıklıgöl, Şanlıurfa. Based on their enzim production capacities, six bacterial isolates designated as BGL-22, BGL-26, BGL-27, BGL-37, BGL-38 and BGL-39 were selected for further studies. Conventional and molecular identification results showed that the bacteria belonged to Bacillus genus. Among these strains, the highest activities for amylase (11.44 U mL-1), lipase (1.12 U mL-1) and protease (2.61 U mL-1) were determined for Bacillus sp. BGL-37. Enzymatic characterization studies demonstrated that the activities of acid-stable amylase and alkaline-stable lipase remained unchanged up to 50℃, while alkaline-protease was retained about 90% of its activity up to 40℃. The findings suggested that these enzymes providing environmentally compatible processes under relatively mild conditions have potential to be used in several fields such as food processing and detergent industry.
Bu çalışmada, Balıklıgöl, Şanlıurfa’dan elde edilmiş ve daha önceden çalışılmamış toprak örneklerinden kırk beş bakteriyel izolat elde edilmiştir. Enzim üretim kapasitelerine göre bu izolatlar arasından BGL-22, BGL-26, BGL-27, BGL-37, BGL-38 ve BGL-39 olarak isimlendirilen altı bakteriyal izolat ileriki denemeler için seçilmiştir. Konvansiyonel ve moleküler tanılama sonuçları, bu bakterilerin Bacillus cinsine dahil olduğunu göstermiştir. Bu suşlar arasından Bacillus sp. BGL-37, amilaz (11.44 U mL-1), lipaz (1.12 U mL-1) ve proteaz (2.61 U mL-1) açısından en yüksek aktiviteyi göstermiştir. Enzimatik karakterizasyon çalışmaları, alkali proteazın 40ºC’ye kadar yaklaşık %90 aktivitesini koruduğunu, asit-kararlı amilaz ve alkali-kararlı lipazın ise 50ºC’ye kadar aktivitelerinde herhangi bir düşüş yaşanmadan aktivite gösterdiklerini ortaya koymuştur. Elde edilen bulgular, nispeten ılımlı koşullar altında çevresel olarak uyumlu süreçlerin gerçekleşmesini sağlayan bu enzimlerin, gıdaların işlenmesi ve deterjan endüstrisi gibi çeşitli alanlarda kullanılma potansiyeline sahip olduğunu göstermiştir.
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Primary Language en
Subjects Biology
Journal Section RESEARCH ARTICLE
Authors

Orcid: 0000-0002-4022-3845
Author: Ebru UYAR (Primary Author)
Institution: Faculty of Arts and Science, Department of Biology, Molecular Biology Section, Harran University
Country: Turkey


Orcid: 0000-0001-8697-0945
Author: Cengiz ÇORBACI
Institution: Faculty of Engineering, Department of Genetics and Bioengineering, Giresun University,
Country: Turkey


Supporting Institution the Scientific Research Unit of Harran University
Project Number Project No: HUBAK-12007
Thanks This study was supported by the Scientific Research Unit of Harran University (Project No: HUBAK-12007). We also thank MedSanTek for performing sequence analysis of PCR products.
Dates

Application Date : October 12, 2020
Acceptance Date : December 3, 2020
Publication Date : August 31, 2021

APA Uyar, E , Çorbacı, C . (2021). Partial Characterization of Hydrolytic Enzymes Produced by Bacillus Strains Isolated from Balıklıgöl, Turkey . Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 24 (4) , 707-714 . DOI: 10.18016/ksutarimdoga.vi.809131