The Effect of Sample Amount and Decoction Time on The Phytochemicals and Antioxidant Activities of Decoction Lemon Balm and Sage

Cennet Yaman

doi:10.18016/ksutarimdoga.vi.810689

EN TR

The Effect of Sample Amount and Decoction Time on The Phytochemicals and Antioxidant Activities of Decoction Lemon Balm and Sage

Abstract

In this study, decoctions (herbal tea) of sage (Salvia officinalis L.) and lemon balm (Melissa officinalis L), two of the important medicinal plants, were analysed for antioxidant activity, antioxidants, and minerals (K, Na, Mg, Ca, Fe, Ba, Ag and Ga) were determined in each sage and lemon balm dry leaves as well as decoctions by inductively coupled plasma-mass spectrometry (ICP-MS). The antioxidant activities of decoctions were evaluated by scavenging activities against 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and hydrogen peroxide (H2O2) radicals, and varied from 19.4 to 109.1 mg trolox equivalent (TE) cup-1 for DPPH and from 35.0 to 168.0 mg ascorbic acid equivalent (AAE) cup-1 for H2O2. The highest antioxidant activity and antioxidants such as total phenolic, flavonoid and flavanol contents were found in the decoction of lemon balm. The analysed minerals were most efficiently observed in sage decoctions. The study also showed that the best sample amount in terms of the minerals was 3 g for both species, but decoction time was statistically insignificant for sage and 10 min more effective for lemon balm. As a result, it was tried to determine the most appropriate sample amount and decoction time for the decoctions of both species according to the analysed parameters.

Keywords

Tıbbi Adaçayı ve Oğul Otu Dekoksiyonlarının Fitokimyasalları ve Antioksidan Aktiviteleri Üzerine Örnek Miktarı ve Dekoksiyon Süresinin Etkisi

Öz

Bu çalışmada, önemli tıbbi bitkilerden olan tıbbi adaçayı (Salvia officinalis L.) ve oğul otu (Melissa officinalis L.) dekoksiyonlarının antioksidan aktivitesi ve antioksidantları analiz edilmiştir. Ayrıca bu türlerin yaprak ve dekoksiyonlarındaki mineral içerikleri (K, Na, Mg, Ca, Fe, Ba, Ag and Ga) indüktif eşleşmiş plazma kütle spektrometresi (ICP-MS) tarafından tespit edilmiştir. Dekoksiyon örnekleri farklı örnek miktarı ve dekoksiyon süresi uygulayarak elde edilmiştir. Dekoksiyonların ntioksidan aktivitesi 1,1-difenil-2-pikril-hidrazil (DPPH) ve hidrojen peroksit (H2O2) radikalleri ile belirlenmiş, DPPH radikalli için 19.4 - 109.1 mg trolox eşdeğeri (TE) / fincan ve H2O2 radikali için 35.0 - 168.0 mg askorbik asit eşdeğeri (AAE) / fincan arasında değişmiştir. En yüksek antioksidan aktivite ve toplam fenolik, flavonoid ve flavanol gibi antioksidantlar oğul otu dekoksiyonlarından elde edilmiştir. Analiz edilen mineraller en verimli tıbbi adaçayı dekoksiyonlarında gözlenmiştir. Ayrıca mineraller açısından en iyi örnek miktarının her iki tür için 3 g olduğunu, ancak dekoksiyon süresinin tıbbi adaçayı için istatistiksel olarak önemsiz ve limon balsamı için 10 dk uygulamanın daha etkili olduğunu saptanmıştır. Sonuç olarak, analiz edilen parametrelere göre her iki türün dekoksiyonları için en uygun örnek miktarı ve kaynatma süresi belirlemeye çalışılmıştır

Anahtar Kelimeler

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Details

Primary Language

English

Subjects

Agricultural, Veterinary and Food Sciences

Journal Section

Research Article

Authors

Cennet Yaman ^*
0000-0002-2364-8171
Türkiye

Publication Date

August 31, 2021

Submission Date

October 15, 2020

Acceptance Date

December 3, 2020

Published in Issue

Year 2021 Volume: 24 Number: 4

DOI

https://doi.org/10.18016/ksutarimdoga.vi.810689

IZ

https://izlik.org/JA87SZ68SW

Cite

RIS / Bibtex

APA

Yaman, C. (2021). The Effect of Sample Amount and Decoction Time on The Phytochemicals and Antioxidant Activities of Decoction Lemon Balm and Sage. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 24(4), 725-732. https://doi.org/10.18016/ksutarimdoga.vi.810689

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