Year 2021, Volume 24 , Issue 4, Pages 689 - 700 2021-08-31

Protective Effects of Lyophilized Ethanolic Extract of Achillea arabica Kotschy. on the Islet β Cells of Streptozotocin-Induced Diabetic Rats
Achillea arabica Kotschy. Liyolifilize Etanolik Ekstresinin Streptozotosin ile Diyabet Oluşturulan Ratların Adacık  Hücreleri Üzerindeki Koruyucu Etkileri

Hanife HANALP [1] , Burak KAPTANER [2] , Abdulahad DOĞAN [3]


In this study, an evaluation of the protective effect of lyophilized ethanolic Achillea arabica Kotschy. extract was investigated on the islets of Langerhans in streptozotocin (STZ)-induced diabetic rats. The rats were divided randomly into 5 groups: Group I: control; Group II: rats treated with STZ (50 mg kg-1 body weight (bw)); Group III: rats treated with A. arabica (Aa) extract (400 mg kg-1 bw); Group IV: rats with DM treated with Aa extract; and Group V: rats with DM treated with glibenclamide (2 mg kg-1 bw) which is a standard drug used in the treatment of DM. STZ treatment caused histopathological changes in the islet and significantly lowered the islet diameter/area, -cell index values, and blood insulin and C-peptide levels. Administration of the extract of Aa improved the islet histology, diameter/area, and -cell index values, and caused significant increases in insulin and C-peptide levels similar to the rats treated with glibenclamide when compared to the diabetic rats. Our findings suggested that the Aa extract possessed protective potential on the pancreatic islets due to its repairing or regeneration impact on -cells.
Bu çalışmada, Achillea arabica Kotschy. liyofilize etanolik ekstresinin, streptozotosin (STZ) ile diyabet oluşturulan ratlarda Langerhans adacıkları üzerindeki koruyucu etkisi araştırıldı. Ratlar rastgele olarak 5 gruba ayrıldı; Grup I: kontrol; Grup II: STZ (50 mg kg-1 vücut ağırlığı (va)) uygulanan ratlar; Grup III: A. arabica (Aa) ekstresi (400 mg kg-1 va) ile muamele edilen ratlar; Grup IV: Aa ekstresi ile muamele edilen diyabetik ratlar ve Grup V: diyabet tedavisinde standart bir ilaç olarak kullanılan glibenklamid (2 mg kg-1 va) uygulanan diyabetik ratlar. STZ uygulamasının, adacıklarda histopatolojik değişimlere neden olduğu, adacık çapını / alanını, -hücre indeksini ve kan insulin ve C-peptid seviyelerini anlamlı olarak düşürdüğü belirlendi. Aa ekstre uygulamasının, glibenklamid ile tedavi edilen ratlara benzer olarak, adacık histolojisinde, çap / alanında, -hücre indeks değerlerinde, kan insulin ve C-peptid seviyelerinde, diyabetik ratlar ile kıyaslandığında, iyileşmeye neden olduğu gözlendi. Elde edilen bulgular Aa ekstresinin pankreas adacıklarında, -hücre onarımını ve yenilenmesini sağlayarak, koruyucu potansiyele sahip olduğunu göstermektedir.
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Primary Language en
Subjects Biology
Journal Section RESEARCH ARTICLE
Authors

Orcid: 0000-0001-5235-4167
Author: Hanife HANALP
Institution: YÜZÜNCÜ YIL ÜNİVERSİTESİ, FEN FAKÜLTESİ
Country: Turkey


Orcid: 0000-0003-2366-6756
Author: Burak KAPTANER (Primary Author)
Institution: VAN YÜZÜNCÜ YIL ÜNİVERSİTESİ
Country: Turkey


Orcid: 0000-0002-5438-8560
Author: Abdulahad DOĞAN
Institution: ECZACILIK FAKÜLTESİ
Country: Turkey


Supporting Institution The Scientific Research Projects Coordination Unit of Van Yuzuncu Yil University
Project Number TYL-2019-865 and FAP-2019-8503
Thanks This study was partially supported by the Scientific Research Projects Coordination Unit of Van Yuzuncu Yil University under project numbers TYL-2019-865 and FAP-2019-8503. Thanks are given to Dr. Süleyman Mesut Pınar for his helpful taxonomical identification of the plant material.
Dates

Application Date : October 15, 2020
Acceptance Date : December 3, 2020
Publication Date : August 31, 2021

APA Hanalp, H , Kaptaner, B , Doğan, A . (2021). Protective Effects of Lyophilized Ethanolic Extract of Achillea arabica Kotschy. on the Islet β Cells of Streptozotocin-Induced Diabetic Rats . Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 24 (4) , 689-700 . DOI: 10.18016/ksutarimdoga.vi.811253